• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.109-113
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• 2003

Cryopreservation of embryogenic callus derived from apical meristem culture was attempted by slow prefreezing method (two-step method) with various cryoprotectants in sweetpotato cv. 'Yulmi' Precultured embryogenic calli on medium containing 10 mg/L ABA prior to slow prefreezing in liquid nitrogen indicated higher survival rate than 1.0 mg/L ABA preteatment. The cryoprotectant comprising 1.28 M DMSO in 0.4 M sucrose solution gave the best survival (over 46%) of sweetpotato cells exposed to liquid nitrogen as determined by TTC reduction and FDA staining method. Cryopreserved calli cultured on MS medium with 1.0 mg/L 2,4-D were grown for 4 weeks in the dark and induced embryos after another 4 weeks. They were subcultured on MS medium supplemented with 0.1 mg/L 2,4-D＋0.1 mg/L kinetin for 2 weeks and regenerated into normal plantlets in MS basal medium.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.101-103
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• 2016

Recently, the target object can be represented as sparse coefficient vector in visual tracking. Due to this reason, exploitation of the compressibility in the transform domain by using L1 minimization is needed. Further, L1 minimization is proposed to handle the occlusion problem in visual tracking, since tracking failures mostly are caused by occlusion. Furthermore, there is a weighted parameter in L1 minimization that influences the result of this minimization. In this paper, this parameter is analyzed for occlusion problem in visual tracking. Several coefficients that derived from median value of the target object, mean value of the arget object, the standard deviation of the target object are, 0, 0.1, and 0.01 are used as weighted parameter of L1 minimization. Based on the experimental results, the value which is equal to 0.1 is suggested as weighted parameter of L1 minimization, due to achieved the best result of success rate and precision performance parameter. Both of these performance parameters are based on one pass evaluation (OPE).

• Journal of Korean Society of Forest Science
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• v.78 no.4
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• pp.401-411
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• 1989

The embryos of Pinus taeda, P. rigida, and P. taeda ${\times}$ rigida were cultured for adventitious shoot regeneration in vitro. Culture media were modified from Gresshoff and Doy (MGD), Murashige and Skoog (MMS), Lloyd and McCown (MLM), and Schenk and Hildebrandt (MSH). NAA was added to initiation media at a concentration of 0.1 or 0.01 mg/l. BAP was used at the concentrations of 0.1. 0.5, 1, 2, or 5mg/l. Each explant was induced for 3-4 weeks on solid medium. All explants were cultured up to 16 weeks. Illumination was about $1506{\pm}540lux$ at the level of the tissues in the growth room with a temperature of $25{\pm}2^{\circ}C$. A 16-hour photoperiod per 24 hours was used. Half-strength medium was used for all the subcultures. For shoot production by loblolly pine, MMS, MLM, or MSH is preferred with 5 mg/l BAP with either 0.1 or 0.01 mg/l NAA. For shoot production by pitch pine, MMS, MLM, or MSH is recommended with 2 or 5 mg/l BAP with 0.1 mg/l NAA. For shoot production by the hybrid pine, MMS or MLM is more effective with 1, 2 or 5 mg/l BAP with 0.1 mg/l NAA. There were no differences recognized among the species tried in the patterns of bud formation and shoot development. Different composition of media, in major and minor salts or possibly in vitamins, should be tested for the two developmental stages of adventitious shoots ; the induction of shoot buds and the elongation of them into shoots.

• The Journal of Korean Medicine
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• v.26 no.2 s.62
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• pp.241-251
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• 2005

Objectives: This experimental study was designed to investig:ue the effects of Oryungsan-gagampang on leptin and leptin receptor levels and differentiation of 3T3-L1 adipocyte. Methods: After 3T3-L1 adipocytes were incubated with various concentrations of Oryungsan-gagampang and Reductil(r) for 7 days, leptin and leptin receptor levels in 3T3-Ll adipocytes were measured by ELISA. To elucidate the mechanism of inhibitory effects of Oryungsan-gagampang on obesity, the 3T3-L1 adipocytes after oil red 0 staining were taken by digital photo system. Results: 1. Oryungsan-gagampang $1,000{\mu}g/ml$ significantly increased leptin levels in 3T3-L1 adipocytes in comparison with the control group (p<0.05), and Oryungsan-gagampang 0.1 10, $1,000{\mu}g/ml$ significantly increased leptin receptor levels in 3T3-L1 adipocytes in comparison with the control group (p<0.05). 2. Oryungsan-gagampang inhibited of differentiation of 3T3-L1 adipocytes. Conclusions: Oryungsan-gagampang showed significant effects on inhibiting differentiation of 3T3-Ll adipocytes, and increasing leptin levels and leptin receptor levels in 3T3-L1 adipocytes. Therefore, Oryungsan-gagampang could be used to treat obesity, but further studies are required.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.38-42
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• 2008

This study was conducted to investigate distribution of selenium in Gangwon highland Province and to confirm the effect of sodium selenate added nutrient solution on the selenium content of some western vegetables; beet, broccoli, cabbage lettuce, celery, and parsley. The contents of selenium were distributed from 0.024 to $0.038\;mg{\cdot}kg^{-1}$ in soil of Gangwon highland province. As the selenium content in soil increased, the selenium content in broccoli was increased in highland of Gangwon province. The selenium content of broccoli showed $10.5{\sim}17.9\;mg{\cdot}kg^{-1}DW$ 1311owe4 by celery ($6.02\;mg{\cdot}kg^{-1}DW$). Beet, cabbage lettuce, and parsley contained just about $1\;mg{\cdot}kg^{-1}DW$ in highland of Gangwon province. Some western vegetables grown by fertigation supplied $2\;mg{\cdot}L^{-1}$ sodium selenate were shown better growth and higher selenium content than those of $0\;mg{\cdot}L^{-1}$ sodium selenate supplied treatment(control). The selenium contents of western vegetables supplied $2\;mg{\cdot}L^{-1}$ sodium selenate in fertigation culture for 60 days were $76.2\;mg{\cdot}L^{-1}$ in broccoli, $69.1\;mg{\cdot}L^{-1}$ in parsley, $63.2\;mg{\cdot}L^{-1}$ in celery, $54.2\;mg{\cdot}L^{-1}$ in beet, $8.3\;mg{\cdot}L^{-1}$ in cabbage lettuce. Those selenium content of broccoli, celery, and parsley treated $2\;mg{\cdot}L^{-1}$ sodium selenate were followed the order 4.2 times, 10.5 times and 62.5 times higher in compared to control.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.23-28
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• 2009

This study was conducted to examine the effect of plant growth regulators on the formation of adventitious bud callus(ABC) and plant regeneration in shoot tip culture of Zantedeschia spp. 'Florex Gold'. Treatment of $0.1mgL^{-1}\;N$-phenyl-N'-1,2,3-thiadiazol-5-ylurea(thidiazuron, TDZ) was more promotive for formation of ABC in shoot tip culture than 6-benzylaminopurine(BA) treatment, and short shoots were developed. Comparing to treatment of BA, mixing treatment of BA and 1-naphthaleneacetic acid(NAA) inhibited the formation of ABC and multiple shoot. The proliferation of ABC derived from sections(0.3 cm) of ABC produced by shoot tip culture in medium supplemented with $0.1mgL^{-1}\;TDZ$ was more effective in medium with $0.1mgL^{-1}\;TDZ$ or $2.0mgL^{-1}\;BA$ than the other treatments. The shoot regeneration and growth from sections of ABC was more promotive in treatment of $0.001mgL^{-1}\;TDZ$. Also, the root growth from sections of ABC was better in medium with $0.001mgL^{-1}\;TDZ$ or $0.2mgL^{-1}\;BA$. Consequently, in vitro mass production of Zantedeschia spp. 'Florex Gold' can be obtained via indirect organogenesis through plant regeneration and proliferation of ABC which was derived from shoot tip culture at $0.1mgL^{-1}\;TDZ$.

• Environmental Analysis Health and Toxicology
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• v.24 no.3
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• pp.193-202
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• 2009

In this study, we analyzed the blood cadmium concentration in general population of Korea, and followed the analyses the relations with epidemiologic factors, life-style and body iron store. The geometric mean concentration of blood cadmium was 1.20 ${\mu}g/L$ in study subject, the level of blood cadmium was higher in female (1.27 ${\mu}g/L$) than in male (1.11 ${\mu}g/L$). The mean concentration of blood cadmium was the highest in coastal area (1.80 ${\mu}g/L$) and followed in urban (1.01 ${\mu}g/L$) and rural area (0.90 ${\mu}g/L$). The cadmium concentration in whole blood was increased with age-dependent pattern, such as 0.64 ${\mu}g/L$ in the below 30 years, 0.95 ${\mu}g/L$ in the 30~44 years, 1.28 ${\mu}g/L$ in the 45~59 years and 1.31 ${\mu}g/L$ in the over 60 years. The level of blood cadmium was higher in smokers than in non-smokers, but was not significant by alcoholic habit. The blood cadmium level was different from occupations, which was the highest in the fishers as 1.87 ${\mu}g/L$. The inversed relation was observed between blood cadmium and serum Fe, while was correlated positively with the ferritin level. In summary, the environmental exposure to cadmium in Korean is not so much compared to other countries, and the blood cadmium is influenced by genetic factors (age and sex), life-styles (dietary habit, occupation and smoking habit) and nutrition such as serum iron.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2005.04a
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• pp.131-148
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• 2005

This study is peformed to investigate the effect of water extract from Vaccinium uliginosum L., on melanin production in B 16 melanoma cells, procollagen production and matrix metalloproteinase-1(MMP-1) inhibition in human fibroblast cells. One hundred grams of the Vaccinium uliginosum L. was extracted with 2000 mL of water($90^{\circ}C$, 16h, 2times). The water extracts were lyophilized and stored at $4^{\circ}C$ until used. Dry weight yields of extracts of Vaccinium uliginosum L. were $3\%$(w/w). Extracts from Vaccinium uliginosum L. showed scavenger activities on DPPH radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide and singlet oxygen radical. And these substances inhibited release of cyiokines from human keratinocyte after UV B exposure. Therefore we confirmed that extracts from Vaccinium uliginosum L. had antioxidative effect. These substances inhibited purified tyrosinase activity and melanogenesis in B 16 melanoma cells treated/untreated IL-$1{\alpha}$. Moreover this extract stimulated procollagen production and inhibited MMP-1 production in human fibroblast cells treated/untreated IL-$1{\beta}$. Therefore we confirmed that extracts from Vaccinium uliginosum L. had whitening effect. And these substances decreased degree of wrinkle in hairless mouse skin that induced by UV B irradiation. Therefore we confirmed that extracts from Vaccinium uliginosum L. had anti-wrinkle effect. From the above results, it is possible that Vaccinium uliginosum L. may be developed to be an anti-melanogenesis agent and anti-wrinkle agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1257-1261
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• 2004

Antioxidant activities of traditional liquors produced in Chungcheong-do were studied. The contents of organic acids, carbohydrate, and protein of the traditional liquors were 0.30~0.95%, 1.4~11.1% and 1.5~37.3 mg%, respectively. Especially, the contents of phenolic compounds of L-3 was the highest (205.8 $\mu\textrm{g}$/mL). L-5, L-4 and L-3 showed higher antioxidant activities than the others on hemoglobin-induced linoleic acid system. L-5 also showed high reducing power and scavenging effect on DPPH radical. Scavenging effects on hydroxyl radicals in L-6, L-5 and L-4 showed higher activities (42~53%) than those of the others. The highest scavenging effect on superoxide anion radical was observed in L-5 ($IC_{50}$/: 1.1 $\mu\textrm{g}$).

• Journal of Veterinary Clinics
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• v.14 no.1
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• pp.1-5
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• 1997

This experiment was performed to investigate the leptospiral antibody in dairy cattle with serological test in three areas of Kangwondo from February to June, 1995. Twelve different living antigen's (S icterohemorrhagiae, Lpomona, L hardjo, L australis, L canicola, L autumnalis, L grippotyphosa, L tarassovi, L pyroenes, L bataviae, L hebdomadis and L ballum) of Leptospira interrogans were used for the serological test in dairy cattle. The blood samples of 130 cattle were examinde by microscopic agglutination test. Among the serum samples of the dairy cattle, 5 heads of the dairy cattle (3.8%) were positive. Among the positive samples of 5 heads, 3 heads of dairy cattle (2.3%) showed the antibody of L canicola, 2 heads of dairy cattle (1.5%) showed the antibody of L icterohaemorrhagiae. Positive rate by age was 0% ($\leq $2year), 4.6% (3~4year) and 6.6% ($\geq $5year).