• Title/Summary/Keyword: L.D.V

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Direct Fermentation of D-Xylose to Ethanol by Candida sp. BT001

  • LEE, SANG-HYEOB;WON-GI BANG
    • Journal of Microbiology and Biotechnology
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    • v.4 no.1
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    • pp.56-62
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    • 1994
  • A yeast strain, BT001, which can directly ferment D-xylose to ethanol was isolated from forest soils, and then identified as Candida sp. Cultural conditions for the optimum ethanol production, along with the effects of aeration on cell growth and ethanol production were investigated. Aeration stimulated the cell growth and the volumetric rate of ethanol production, but decreased the ethanol yield. Optimum temperature and initial pH for the ethanol production were $33{\circ}^C$ and 6.0, respectively. In a shake flask culture, this strain produced 52.3 g ethanol per liter from 12%(w/v) D-xylose after incubation for 96 hours. Ethanol yield was 0.436 g per g D-xylose consumed. This corresponds to 85.8% of theoretical yield. Also, this yeast strain produced ethanol from D-galactose, D-glucose and D-mannose, but not from L-arabinose and L-rhamnose. Among these sugars, D-glucose was the fastest in being converted to ethanol sugars.

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Flavonoids from Salix hallaisanensis Leaves (떡버들 잎의 플라보노이드)

  • Oh, Mi-Hyun;Ham, In-Hye;Chung, Sung-Hee;Whang, Wan-Kyun
    • Korean Journal of Pharmacognosy
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    • v.36 no.4 s.143
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    • pp.282-290
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    • 2005
  • The MeOH extract of the the leaves of Salix hallaisanensis (Salicaceae) was partitioned successively with $CHCl_3$, 20% MeOH, 40% MeOH and 60% MeOH solution. From the fractions obtained, 9 compounds were isolated, $diosmetin-7-O-{\beta}-d-glucoside$ (I), $diosmetin-7-O-{\beta}-D-glucosyl-(1{\rightarrow)6)-{\beta}-d-glucoside$ (II), $diosmetin-7-O-{\beta}-d-xylosyl-(1{\rightarrow}6)-{\beta}-D-glucoside$ (III), $quercetin-3-O-{\beta}-d-galactoside$ (hyperoside) (IV), $quercetin-3-O-{\alpha}-l-rhamnosyl-(1{\rightarrow}6)-{\beta}-D-glucoside(rutin)$ (V), luteolin (VI), $luteolin-7-O-{\beta}-d-glucoside$ (VII), $kaempferol-3-O-{\alpha}-l-rhamnosyl-(1{\rightarrow}6)-{\beta}-D-glucoside$ (VIII), and (+)-catechin (IX).

Electrical Transport Properties of La2/3TiO2.84 Ceramic (La2/3TiO2.84 세라믹스의 전기전도특성)

  • Jung, Woo-Hwan
    • Journal of the Korean Ceramic Society
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    • v.41 no.11
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    • pp.858-863
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    • 2004
  • The thermoelectric power, dc conductivity and magnetic properties of the cubic L $a_{2}$ 3/Ti $O_{2.84}$ were investigated. The thermoelectric power was negative below 350 K. The measured thermoelectric power of L $a_{2}$ 3/Ti $O_{2.84}$ increased linearly with temperature, in agreement with model proposed by Emin and Wood, and was represented by A+BT. Temperature dependence indicates that the charge carrier in this material is a small polaron. L $a_{2}$ 3/Ti $O_{2.84}$ exhibited a cross over from variable range hopping to small polaron hopping conduction at a characteristic temperature well below room temperature. The low temperature do conduction mechanism in L $a_{2}$ 3/Ti $O_{2.84}$ was analyzed using Mott's approach. Mott parameter analysis gave values for the density of state at Fermi level [N( $E_{F}$)] = 3.18${\times}$10$^{20}$ c $m^{-3}$ e $V^{-1}$ . The disorder energy ( $W_{d}$) was found to be 0.93 eV, However, it was noted that the value of the disorder energy was much higher than the high temperature activation energy. The exist linear relation between log($\sigma$T)와 1/T in the range of 200 to 300 K, the activation energy for small polaron hopping was 0.15 eV.

Degradation Characteristics by Hot Carrier Injection of nchannel MOSFET with Gate- $n^{-}$S/D Overlapped Structure (게이트와 $n^{-}$소스/드레인 중첩구조를 갖는 n 채널 MOSFET의 핫캐리어 주입에의한 소화특성)

  • 이대우;이우일
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.30A no.2
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    • pp.36-45
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    • 1993
  • The n-channel MOSFETs with gate-$n^{-}$S/D overlapped structure have been fabricated by ITLDD(inverse-T gate lightly doped drain) technology. The gate length(L$_{mask}$) was 0.8$\mu$m. The degradation effects of hot carriers injected into the gate oxide were analyzed in terms of threshold voltage, transconductance and drain current variations. The degradation dependences on the gate voltage and drain voltage were characterized. The devices with higher n-concentration showed higher resistivity against the hot carrier injection. As the results of investigating the lifetime of the device, the lifetime showed longer than 10 years at V$_{d}$ = 5V for the overlapped devices with the implantation of an phosphorus dose of 5$\times$10$^{13}$ cm$^{-2}$ and an energy of 80 keV in the n$^{-}$resion.

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Efficient Isolation of Dihydrophaseic acid 3'-O-β-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography

  • Rho, Taewoong;Yoon, Kee Dong
    • Natural Product Sciences
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    • v.24 no.4
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    • pp.288-292
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    • 2018
  • High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.

INTRACEREBROVENTRICULARLY ADMINISTERED PHENYLALANINE AND TYROSINE: EFFECTS ON FEEDING BEHAVIOUR AND NOREPINEPHRINE CONCENTRATIONS OF SPECIFIC BRAIN SITES IN THE CHICKEN

  • Choi, Y.-H.;Furuse, M.;Okumura, J.;Shimoyama, Y.;Sugahara, K.;Denbow, D.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.3
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    • pp.255-259
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    • 1996
  • A study was carried out to investigate the action of central L-pheylalanine (Phe) and L-tyrosine (Tyr) on food intake of the chicken. In the first trial, Phe ($200{\mu}g/10{\mu}l$) or saline was acutely administered into the right lateral ventricle (i.c.v.) of chickens (5 birds per each group). Birds (4 birds per each group) were administered with the i.c.v. Tyr ($200{\mu}g/10{\mu}l$) or saline in the second trial. The brains of the birds were removed for catecholamine assy 30 min postadministration. Catecholamine concentrations were measured at specific sites of the brain (LH: lateral hypothalamus, PVN: paraventricular nucleus, and VMH: ventromedial hypothalamus). No significant effect of amino acids on the concentration of norepinephrine of brain sites investigated was detected. Food intake and rectal body temperature were also monitored for 6 h after central administrations of Phe, Tyr or saline (5 birds per each group). Both Phe and Tyr, up to $1mg/10{\mu}l$, failed to modulate food intake or rectal body temperature.

Phase Cooperation Between Mo-V-O and Metal Oxide in Selective Oxidation of Acrolein (아크롤레인 선택 산화반응에서 Mo-V-O와 금속산화물의 상간협동)

  • Park, D.W.;Na, S.E.;Kim, K.H.;Lee, W.H.;Chung, J.S.
    • Applied Chemistry for Engineering
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    • v.5 no.2
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    • pp.327-336
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    • 1994
  • The synergistic effects in mechanical mixture catalysts of Mo-V-O and metal oxide were investigated for the selective oxidation of acrolein. The metal oxides used are $SnO_2$, ${\alpha}-Sb_2O_4$, $WO_3$, ${\alpha}-Al_2O_3$, CuO, $MnO_2$, $Cu_2O$, MgO, CoO, and ZnO. Mechanical mixtures of Mo-V-O plus $SnO_2$ or ${\alpha}-Sb_2O_4$ had resulted in higher conversion of acrolein and higher yield of acrylic acid than Mo-V-O. The origin of the synergy is attributed to the cooperation of Mo-V-O and $SnO_2$ or ${\alpha}-Sb_2O_4$, in which $SnO_2$ or ${\alpha}-Sb_2O_4$ forms dissociated oxygens at their oxygen vacancies and transports them to Mo-V-O. $Cu_2O$, MgO, CuO, and $MnO_2$, increased conversion of acrolein but decreased yield of acrylic acid. CoO and ZnO inhibited the catalytic performance of Mo-V-O. The different role of these metal oxides is explained in terms of their oxidation-reduction properties.

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Pharmacokinetics of a New PEG-hemoglobin SBI for Single Intravenous Administration to Rats (흰쥐에 있어서 PEG-헤모글로빈 SBI의 단회정맥투여 약물동태)

  • 신호철;심희옥;유승룡;김종춘;양승돈;정문구
    • YAKHAK HOEJI
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    • v.44 no.3
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    • pp.232-236
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    • 2000
  • An intravenous pharmacokinetics for a new red cell substitute, PEG-hemoglobin SB1, was studied in SD rats. Total-hemoglobin and its metabolite methemoglobin in the plasma were determined using a spectrophotometer. The limit of quantitation was 0.01g/dL and the C.V for interday assay reproducibility was less than 6%. Upon intravenous administration of anticipated clinical dose, 10 ml(0.7 gHb)/kg, plasma concentration curve of total-hemoglobin was well described by one-compartment model. The $t_{\frac{1}{2}},{\;}CL_{t}$, Vd and $AUC^{0-48hr}$ were $8.23{\pm}0.96{\;}hr,{\;}0.06{\;}{\pm}{\;}0.01 {\;}dL/hr/kg,{\;}0.66{\pm}0.05{\;}dL/lg{\;}and{\;}13.6{\;}{\pm}{\;}1.01g{\cdot}hr/dL$, respectively, in male rats(n=5, $mean{\;}{\pm}{\;}SD$). Those parameters in female rats were $9.21{\;}\pm{\;}2.31{\;}hr,{\;}0.06{\;}{\pm}{\;}0.01{\;}dL/hr/kg,{\;}0.79{\pm}{\;}0.08{\;}dL/kg{\;}and{\;}13.0{\;}{\pm}{\;}2.36{\;}g{\cdot}hr/dL$, respectively. Similar kinetic profiles between males and females were also obtained from other parameters. Small amount of methemoglobin, an oxidative metabolite of SB1, was detected in the plasma of both sexes, where the $AUC^{0-48{\;}hr,m}$ and $t_{{\frac{1}{2}},m}$ were approximately $1.5{\;}g{\cdot}hr/dL$ and 20 hr, respectively. The present work provides a critical kinetic data for the effective clinical applications of PEG-hemoglobin SB1.

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Local Convective Mass Transfer and Flow Structure Around a Circular Cylinder with Annular Fins (환상핀이 부착된 원봉 주위의 3차원 박리 유동구조 및 물질전달 특성 해석)

  • 박태선;성형진
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.15 no.6
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    • pp.2132-2146
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    • 1991
  • Extensive experiments were carried out to investigate the mass transfer and flow structures around a circular cylinder with annular fins in crossflow. The naphthalene sublimation method was employed to measure the circumferential and longitudinal variations of mass transfer from the circular cylinder between annular fins and H is the height of the fin from the cylinder surface. A remarkable enhancement of mass transfer due to the horseshoe vortices was observed near the corner junction between the annular fin and circular cylinder. The present results indicate that the local circumferential Sherwood number shows the higher values on the front stagnation point. The maximum augmentation of mass transfer rate at the center of cylinder is found near L/H-0.15 due to the separation bubble along the annular fins. The secondary flows, which are the corner vortices V2 and V3 near the end wall of the annular fin, are fairly predicted from the distributions of local Sherwood number in the spanwise direction. The average Sherwood number of overall surface at L/H=0.15 is larger 2.0 times than that of without annualr fins. The correlations of total average mass transfer rate with L/H and Re$_{L}$ can also be obtained.d.