• 원예과학기술지
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• 제35권1호
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• pp.88-97
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• 2017

This study explored the physicochemical and nutritional changes associated with storage duration of fresh tomatoes. Fruits of the 'TY Megaton' and 'Yureka' tomato cultivars were harvested at the pink stage and stored at $12^{\circ}C$ for 20 days. During storage, firmness, weight loss, skin color (Hunter L, a, b, a / b values), soluble solids content (SSC), titratable acidity (TA), pH, antioxidant contents (lycopene, ascorbic acid, and total phenolics) and antioxidant activity were evaluated. Firmness was above the minimum marketable limit and fresh weight loss was below maximum acceptable weight loss after 3 weeks of storage, and no deleterious effect on antioxidant contents or activities were observed. Significant differences in SSC, TA, and pH were seen between varieties, but not between fruits stored for different durations. In both varieties, Hunter a values increased more than five-fold after 8 days of storage; this correlated with a more than four-fold accumulation of lycopene after two weeks of storage. The antioxidant activity of tomatoes was highest at the beginning of the storage period, likely because of the effective DPPH - reducing power of ascorbic acid and total phenolics. Antioxidant activity increased after 12 days of storage because of increasing lycopene content. Hence, this study indicates that pink - stage tomatoes may be stored at $12^{\circ}C$ for up to 3 weeks without affecting marketability or nutritional value.

• Journal of Plant Biology
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• 제23권2호
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• pp.37-44
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• 1980

Kidneybean(Phaseolus vulgaris L.) and buckwheat(Fagopyrum esculentum M nch) seedlings grown with Hoagland solution to a height of 7 to 10 cm in the earthen pot containing sand were used for experimental plants. One group of the experimental plants was irrigated with Hoagland solution composed of various Pb-concentratons of 0 to 1000ppm containing 30ppm, and the other group was irrigated with Hoagland solution composed of various P-concentrations of 0 to 1,000ppm containing 30ppm Pb for a month during June and July in 1979. By Pb-surplus over 100ppm, the margins of buckwheat were curled down and turned into yellow-brown chlorosis, but the leaves of kidneybean were become dark-green at an early stage, and then developed chlorosis, finally shedded earlier than the control. Pb-toxicity was similar to the symptoms of P-deficiency. The ratio, 3/1 of chlorophyll a/b at an early stage, was unaffected by Pb-surplus or P-deficiency, but at the later stage it was altered. ATP content with Pb-surplus of 100ppm was decreased by 69% in kidneybean leaves and by 38% in buckwheat leaves, and it with P-deficiency was decreased by 75% in kidneybean leaves and by 43% in buckwheat leaves. In the assay of ATP content of the leaves at intervals of three hours for one day of July in the year, the rhythms of ATP level were unaffected by light or temperature although the amplitude of the level was modified. The rhythms of Pb-surplus and P-deficiency plants were observed at the lower range of ATP content than that of control.

• Journal of Plant Biology
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• 제22권1_2호
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• pp.15-20
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• 1979

The present study was carried out to investigate the effects of cadmium(Cd), mercury(Hg) and lead(Pb) on the respiration of the germinating seeds. Two kinds of seeds namely, mungbean (Paseolus radiatus L.) and buckwheat (Fagopyrum esculentum M ench) were used for the plant materials. The concentrations of Cd, Hg, and Pb applied for the treatment were 0.1, 1, 10, 100 and 1,000ppm. The germination test was carried out in the dark at 27$^{\circ}C$. Regardless of the kinds of heavy metals, the respiration of the germinating seeds was greatly increased for 24hrs absorbing the heavy metals, and subsequently the respiration was increased average 0.69mg/g.hr.CO2 for mungbean and 0.7mg/g.hr.CO2 for buckwheat. In the lower concentrations of cadmium and lead than 10ppm the germinating rates, seedling concentraitons. The germinating rates, the seedling growth and the respiration were not increased in ay concentrations of mercury, but were decreased with increase of the mercury concenration. The inhibition of seeds respiration by mercury was 3 times higher than by cadmium, and 5 times higher than that by lead. The toxicity of three heavy metals on the respiration was showed from the highest to the lowest in the order of Hg, Cd, and Pb.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.103-103
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• 2003

This study was carried out to investigate of the biologically active components in germinating Mung bean(Phaseolus radiata L.) and Buckwheat (Fagopyrum esculentum Moench) seeds. During the initial germination, germination ratio of 24 hours pre-soaking Mung bean and Buckwheat seeds were higher about 2∼3% than that of non-soaking. This experiment also was peformed to observe cytotoxic effect of the germinating seeds(germination length : 2, 5, 10mm) extracts against cancer cell lines including human lung carcinoma(Calu-6), human breast adenocarcinoma(MCT-7), human great intestine carcinoma(Caco-2) and human leukemia carcinoma(AML-2/WT). The growth of the cancer cells in medium containing Mung bean and Buckwheat extracts were significantly inhibited degree in proportion to the length of germination seeds, Especially, the results show that a significant shrinkage of Calu-6 cells was observed when the cells were exposed into extract of 10mm germination seeds in germinating Mung bean and Buckwheat seeds.

• 원예과학기술지
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• 제19권3호
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• pp.325-328
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• 2001

종자(種子) 파종에 의한 토마토 실생묘(實生苗)를 대체하고 반드시 제거(除去)해야하는 측지(側枝)를 이용한 삽목묘(揷木苗)를 다량생산(多量生産)하여 종자수입을 대체하고 공정육묘의 가능성을 검토하기 위하여 본(本) 시험(試驗)을 수행(遂行)하였다. 처리는 관행 삽목상과 배지용량 23mL인 트레이 128구부터 300mL 용량의 직경 8cm 비닐폿트를 이용하여 삽목하였다. 그리고 배지의 조성은 훈탄단용과 훈탄과 펄라이트, 훈탄과 피트모스, 펄라이트와 피트모스를 혼용하여 이용하였다. 토마토 삽목묘(揷木苗)의 생육량(生育量)은 배지용량(培地容量)이 120mL 이상으로 증가되어도 크게 영향을 받지 않았다. 그러나 배지 용량이 감소되어 플러그 수가 증가되면 묘(苗)의 생육은 저하되었으나 23mL 용량의 128구 플러그 트레이에서도 정상적인 묘(苗)가 생산되었다. 배지용량이 감소되면 지상부보다 지하부중이 크게 감소되었다. 토마토 측지삽목묘(側枝揷木苗)의 생육은 방울토마토 뻬뻬가 완숙토마토 모모타로 품종보다 양호하고 T/R율이 높았으며, 발근율(發根率)도 뻬뻬 품종은 100%였으나 모모타로는 90% 수준(水準)이었다. 배지용량과 배지종류에 관계없이 삽목후(揷木後) 15-20일경에 정식 가능한 묘가 생산되었다. 삽목 육묘용(育苗用) 배지(培地)의 조성이 발근(發根)과 근발달(根發達)에 미치는 영향은 처리간(處理間)에 유의차(有意差)가 인정되지 않았다.

• Applied Biological Chemistry
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• 제46권2호
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• pp.140-143
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• 2003

본 연구는 지금까지 제시된 여러 가지 간이 식물영양 진단 방법들 중 test strip과 chlorophyll meter의 이용가능성을 검토하고 실제 이용 방안을 모색하고자 수행되었다. 본 실험에서는 양액의 N, P, K수준을 달리하여 토마토를 재배하면서 생육시기별로 specific color difference sensor value (SCDSV), 엽병 즙액 중 $NO_3,\;PO_4,\;K$의 함량을 측정하였다. 실험 결과 양액중 N, P, K의 농도 변화에 따른 엽병 즙액 중 $NO_3,\;PO_4,\;K$ 함량 변화가 엽 중 total-N, E, K함량의 변화보다 더 민감하였다. Test strip을 이용하여 측정한 토마토 엽병 즙액 중 $NO_3,\;PO_4,\;K$ 함량은 엽 내 total-N, E, K의 농도와 고도의 일차상관관계를 나타내었다. 지금까지 여러 연구를 통해 확립된 토마토의 엽 중 total-N, E, K의 적정 함량을 희귀식에 대입하여 구한 즙액의 적정 $NO_3,\;PO_4,\;K$의 함량은 각각 3.4-5.9, 0.3-0.5, 3.6-6.5 g/l였다.. Chlorophyll meter를 이용하여 측정한 SCDSV는 엽 중 total-N와 높은 상관관계를 나타내었으며 기존에 연구된 토마토의 엽 중 total-N 함량을 희귀식에 대입하여 계산한 결과 적정 SCDSV는 36.0-40.0이었다. 본 연구 결과 test strip과 chlorophyll meter는 토마토의 신속한 영양 진단에 이용할 수 있을 것으로 판단된다.

• 식물조직배양학회지
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• 제26권1호
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• pp.41-47
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• 1999

토마토의 genomic DNA library로부터 분리한 tPALl, tPAL4유전자의 염기서열을 분석하여 tPAL5 유전자와 비교 분석한 결과는 다음과 같다. tPAL5 유전자는 722개의 아미노산과 710 bp의 intron을 가지고 있으나 tPALl은 intron을 가지고 있지 않으며 또한 tPAL5 유전자와 비교하여 249개의 짧은 polypeptide를 가지고 있었다. tPAL4유전자인 경우 357개의 아미노산과 305bp의 intron을 가지고 있었다. tPAL 효소간의 아미노산 homology는 tPAL1유전자와 tPAL4 유전자간은 87.2%, tPALl과 tPAL5는 85.3%, tPAL4 와 tPAL5 는 91.4%의 homology를 보였다. 또한, tPALl, tPAL4 유전자는 정상적인 polypeptide를 가지는 tPAL5유전자와 비교하여 비정상적인 stop codon을 가진 짧은 polypeptide로 구성되어 있었다. 다양한 식물 종으로부터 분리된 PAL유전자의 염기서열을 비교한 결과 토마토 (Lycopersicon esculentum), 감자 (Solanum tuberosum), 고구마 (Ipomoea batatas)간의 유연관계과 높았으며, parsley (Petroselinum crispum), bean (Phaseolus vulgaris), pea (Pisum sativum), alfalfa (Medicago sativa) 등이 각각 서로간에 유연관계가 높았다. 또한, 토마토에서 분리한 family내에서 tPAL4와 tPAL5 유전자는 homology가 매우 높았고 (93.0%), tPAL1와 tPAL4유전자 사이는 다소 낮았으며 (84.4%), 특히 tPAL4는 감자의 PAL 유전자와 매우 높았다 (90.6%).

• 생물환경조절학회지
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• 제17권4호
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• pp.283-287
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• 2008

본 연구는 저광 조사 저온 저장 중 광합성유효방사(PAR)내의 각 파장이 토마토 묘의 형태와 엽색 변화에 미치는 영향을 조사하기 위하여 실시되었다. $10{\pm}0.5^{\circ}C$의 온도와 피크 파장이 각각 405, 450, 505, 545, 600, 645, 680, 700nm의 발광다이오드로부터 조사된 $3{\mu}mol\;m^{-2}s^{-1}$의 광합성유효광량 자속밀도 조건에서 토마토 접목묘를 28일간 저장하였다. 405, 450, 505nm의 파장에서 저장된 묘의 잎은 다른 파장에서 저장된 잎에 비해 직립하는 경향을 보였으며, 특히 450과 505nm 파장에서 저장된 묘의 경우 엽축 생장이 억제되면서 콤팩트한 형태를 보였다. 파장의 변화에 따른 엽색의 규칙적인 변화는 저장 전후를 비교하여 관찰되지 않았으나, 405와 700nm 파장에서 저장된 묘는 엷은 녹색을 나타내었다. 저광 조사 저온 저장 중, $450{\sim}545nm$ 영역의 광조사는 토마토 묘의 직립을 유도하여 외관상 건강한 묘로 인정 받는데 기여할 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1622-1628
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• 2006

Bacteria from the Methylobacterium genus, called pink-pigmented facultative methylotrophic bacteria (PPFMs), are common inhabitants of plants, potentially dominating the phyllosphere population, and are also encountered in the rhizosphere, seeds, and other parts of plants, being versatile in nature. The consistent success of the Methylobacterium plant association relies on methylotrophy, the ability to utilize the one-carbon compound methanol emitted by plants. However, the efficiency of Methylobacterium in plant growth promotion could be better exploited and thus has attracted increasing interest in recent years. Accordingly, the present study investigated the inoculation effects of Methylobacterium sp. strains CBMB20 and CBMB 110 on seed imbibition to tomato and red pepper on the growth and accumulation of phytohormone levels under gnotobiotic conditions. Seeds treated with the Methylobacterium strains showed a significant increase in root length when compared with either the uninoculated control or Methylobacterium extorquens $miaA^-$ knockout mutanttreated seeds. Extracts of the plant samples were used for indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), and dihydrozeatin riboside (DHZR) assays by immunoanalysis. The treatment with Methylobacterium sp. CBMB20 or CBMB 110 produced significant increases in the accumulation of IAA and the cytokinins t-ZR and DHZR in the red pepper extracts, whereas no IAA was detected in the tomato extracts, although the cytokinin concentrations were significantly increased. Therefore, this study proved that the versatility of Methylobacterium as a plant-growth promoting bacteria could be better exploited.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2004년도 총회및 심포지움
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• pp.67-72
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• 2004

Quality of fresh-cut tomato slices was compared during cold storage under various modified atmosphere packaging conditions. Chilling injury of slices in containers sealed with Film A was higher than with Film B; these films had oxygen transmission rates of 87.4 and 60.0 ml $h^{-1}\;m^{-2}\;atm^{-1}$ at $5^{\circ}C\;and\;99\%$ RH, respectively. While slices in containers with an initial atmospheric composition of air, $4\%\;CO_2+1\;or\;20\%\;O_2,\;8\%\;CO_2+1\;or\;20\%\;O_2,\;or\;12\%\;CO_2+20\%\;O_2$ showed fungal growth, slices in containers with $12\%\;CO_2+1\%\;O_2$ did not. Low ethylene in containers enhanced chilling injury. Modified atmosphere packaging provided good quality tomato slices with a shelf-life of 2 weeks or more at $5^{\circ}C$. Experiments were conducted to compare changes in quality of slices of red tomato (Lycopersicon esculentum Mill. 'Sunbeam') fruit from plants grown using black polyethylene or hairy vetch mulches under various foliar disease management systems including: no fungicide applications (NF), a disease forecasting model (Tom-Cast), and weekly fungicide applications (WF), during storage at $5^{\circ}C$ under a modified atmosphere. Slices were analyzed for firmness, soluble solids content (SSC), titratable acidity (TA), pH, electrolyte leakage, fungi, yeasts, and chilling injury. With both NF and Tom-Cast fungicide treatments, slices from tomato fruit grown with hairy vetch (Vicia villosa Roth) mulch were firmer than those from tomato fruit grown with black polyethylene mulch after 12 days storage. Ethylene production of slices from fruit grown using hairy vetch mulch under Tom-Cast was about 1.5- and 5-fold higher than that of slices from WF and NF fungicide treatments after 12 days, respectively. The percentage of water-soaked areas (chilling injury) for slices from tomato fruit grown using black polyethylene mulch under NF was over 7-fold that of slices from tomato fruit grown using hairy vetch under Tom-Cast. When stored at $20^{\circ}C$, slices from light-red tomato fruit grown with black polyethylene or hairy vetch mulches both showed a rapid increase in electrolyte leakage beginning 6 hours after slicing. However, slices from tomato fruit grown using the hairy vetch mulch tended to have lower electrolyte leakage than those grown with black polyethylene mulch. These results suggest that tomato fruit from plants grown using hairy vetch mulch may be more suitable for fresh-cut slices than those grown using black polyethylene mulch. Also, use of the disease forecasting model Tom-Cast, which can result in lower fungicide application than is currently used commercially, resulted in high quality fruit for fresh-cut processing. Experiments were conducted to determine if ethylene influences chilling injury, as measured by percentage of slices exhibiting water-soaked areas in fresh-cut tomato slices of 'Mountain Pride' and 'Sunbeam' tomato (Lycopersicon esculentum Mill.). Ethylene concentration in containers without ventilation significantly increased during storage at $5^{\circ}C$, whereas little or no accumulation of ethylene occurred in containers with one or six perforations. Chilling injury was greatest for slices in containers with six perforations, compared to slices in containers with one perforation, and was over 13-fold greater than that of slices in control containers with no perforations. An experiment was also performed to investigate the effectiveness of including an ethylene absorbent pad in containers on subsequent ethylene accumulation and chilling injury. While ethylene in the no-pad controls increased continually during storage of both 'Mountain Pride' and 'Sunbeam' tomatoes at $5^{\circ}C$ under modified atmosphere conditions, no increase in accumulation of ethylene was observed in containers containing ethylene absorbent pads throughout storage. The ethylene absorbent pad treatment resulted in a significantly higher percentage of chilling injury compared with the no-pad control. In studies aimed at inhibiting ethylene production using AVG during storage of slices, the concentration of ethylene in control containers (no AVG) remained at elevated levels throughout storage, compared to containers with slices treated with AVG. Chilling injury in slices treated with AVG was 5-fold greater than that of controls. Further, we tested the effect of ethylene pretreatment of slices on subsequent slice shelf-life and quality. In slices treated with ethylene (0, 0.1, 1, or $10\;{mu}L\;L^{-1}$) immediately after slicing, ethylene production in non-treated controls was greater than that of all other ethylene pre-treatments. However, pretreatment of slices 3 days after slicing resulted in a different pattern of ethylene production during storage. Ihe rate of ethylene production by slices treated with 1 L $L^{-1}$ ethylene 3 days after slicing was greater during storage than any of the other ethylene treatments. With slices pre-treated with ethylene, both immediately and 3 days after slicing, the rate of ethylene production tended to show an negative correlation with chilling injury. Chemical name used: 1-aminoethoxyvinylglycine (AVG).