• 대한본초학회지
• /
• 제24권3호
• /
• pp.59-68
• /
• 2009

Objectives : Bupleuri Radix (Siho) is prescribed as the root of different Bupleurum species on the pharmarcopoeia in Korea and China. Moreover, other species and varieties of the genus Bupleurum have been also distributed on the herbal market as Bupleuri Radix. However, due to the morphological similarity and frequent occurrence of intermediate forms, the correct identification of this radix is very difficult. To develop a reliable method for correct identification and improving the quality standards of official Bupleuri Radix, we analyzed sequences of the ribosomal RNA gene and internal transcribed spacer (rDNA-ITS) region. Methods : PCR amplification of rDNA-ITS region was performed using ITS1 and ITS4 primer from 6 Bupleurum species and 1 variety, B. falcatum L. (Siho), an improved breed of B. falcatum L. (Samdo-Siho), B. chinense DC. (Buk-Siho), B. scorzonerifolium Willd. (Nam-Siho), B. longiadiatum Turcz. (Gae-Siho), B. euphorbiodes Nakai (Deungdae-Siho) and B. latissimum Nakai (Seom-Siho), and nucleotide sequence was determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW using entire rDNA-ITS sequence of three samples per species. Results : In comparative analysis of the rDNA-ITS sequences, we found specific nucleotides to distinguish Korean (B. falcatum L. and its variety) and Chinese official species (B. chinense DC. and B. scorzonerifolium Willd.) from others at positions 411 and 447, and positions 89, 101, 415 and 599, respectively. Futhermore, we also found nucleotide indels (insertion and/or deletion) and substitutions to identify each of different Bupleurum species, 2 positions for B. falcatum L. and its variety, 6 positions for B. chinense DC., 49 positions for B. scorzonerifolium Willd., 8 positions for B. euphorbioides Nakai, 7 positions for B. longiradiatum Nakai and 9 positions for B. latissimum Nakai. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origins of Bupleuri Radix. Moreover, we confirmed the phylogenetic relationship of B. latissimum Nakai, a Korean endemic speices, among Bupleurum species based on the rDNA-ITS sequence. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterant Bupleurum species.

• Natural Product Sciences
• /
• 제24권3호
• /
• pp.159-163
• /
• 2018

Two new polyketides, chinoketides A and B (1 - 2) with a known compound xylarphthalide A (3), were isolated from the solid medium of the endophytes from the leaves of the relic plant Distylium chinense with the "black-box" co-culture method, and the structures of two new compounds were elucidated by NMR, MS and CD spectra. And the absolute configurations of chinoketides A (1) and B (2) were determined as 2R,3R,8S and 5R,6S by calculating their ECD spectra to compare with the experimental CD spectra. Finally, the antimicrobial activities were evaluated to Erwinia carotovora sub sp. Carotovora (Jones) Bersey et al, and the results showed that compounds 1 - 3 displayed the antimicrobial activities with MIC value at 20.5, 30.4 and $10.2{\mu}g/mL$.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 춘계학술발표회
• /
• pp.94-94
• /
• 2018

The gold (LC-AuNPs) and silver (LC-AgNPs) nanoparticles were rapidly synthesized by fruit extract of Lycium chinense within 1.15 and 25 min respectively in an eco-friendly way. The synthesized nanoparticles confirmed by relevant surface plasmon resonance peaks for gold and silver nanoparticles at 536 and 480 nm, respectively. FE-TEM results revealed that LC-AuNPs were 20-50 nm and LC-AgNPs were 50-100 nm. The maximum distribution of gold, silver elements and the crystallographic nature of synthesized were confirmed using EDX, elemental mapping and XRD. LC-AgNPs showed inhibitory activity against pathogenic microorganisms such as E. coli and S. aureus, whereas LC-AuNPs did not show inhibitory activity. The LC-AgNps nanoparticles exhibited significant cytotoxicity to human breast cancer MCF7 cell line and less cytotoxicity to non-diseased RAW264.7 (murine macrophage) cells whereas LC-AuNps showed minimal toxicity to both cell lines. In-depth research on this rapid, facile and greenery nanoparticles may play a potential role in biomedical applications.

• Journal of Plant Biotechnology
• /
• 제29권1호
• /
• pp.15-18
• /
• 2002

구기자나무 (Lycium chinense Mill.)의 절편체 종류, 엽절편체의 착생 부위별 그리고 품종 간 캘러스 형성과 신초분화의 특징을 조사하였다. 기내식물체의 잎과 포장엽 절편체에서의 캘러스는 쉽게 유도되었으나, 기내엽 절편체에서 유도된 캘러스는 배양기간이 경과되면서 딱딱하게 변하였다. 잎의 착생 위치에 따른 캘러스 발생은 포장엽 및 기내엽에서 모두 중간이 양호하였고, 캘러스의 생체중은 기내엽보다는 포장엽 절편체가 더 좋았다. 신초 분화는 중간 부위의 기내엽과 상부위의 포장엽이 양호하였다. 품종간 신초 분화는 '진도재래', '청양', '영하구기자' 그리고 '청양재래' 순으로 양호하였다.

• 한국식품영양과학회지
• /
• 제43권1호
• /
• pp.24-29
• /
• 2014

본 연구는 구기자와 구기엽 추출물이 난소를 절제한 흰쥐의 체중 증가, 골밀도, 에스트로겐 수용체의 발현에 미치는 영향을 밝히고자 시행하였다. 실험군은 흰쥐 24마리를 가장수술군(Sham군), 난소를 절제한 대조군(OVX군), 난소절제 후 구기자 추출액을 투여한 군(LCF군), 난소절제 후 구기엽 추출액을 투여한 군(LCL군)으로 구분하였다. 8주 후 난소를 절제한 OVX군($330{\pm}5.39g$), LCF군($315{\pm}2.99g$) 및 LCL군($318{\pm}2.06g$)의 체중은 Sham군($281{\pm}1.71g$)에 비하여 증가하였다. 혈청 osteocalcin 활성은 OVX군($107{\pm}3.52ng/mL$)에 비하여 LCF군($444.6{\pm}26.9ng/mL$)과 LCL군($407{\pm}18.9ng/mL$)에서 증가하였다. Alkaline phosphatase의 활성은 OVX군($95{\pm}2.9U/L$)에 비하여 LCF군($108{\pm}2.7U/L$)과 LCL군($407{\pm}18.9ng/mL$)에서 증가하였다. 8주 후 넙다리뼈를 실체현미경으로 관찰한 결과 뼈 기질밀도는 난소절제군에서 감소하였으나, 구기자와 구기엽을 추출액을 투여한 군에서는 뚜렷이 회복되었다. 에스트로겐 수용체의 mRNA 발현은 OVX군에서는 거의 발현되지 않았으나, LCF군과 LCL군에서 뚜렷이 증가하였다. 이상의 결과로 보아 구기자와 구기엽 물추출물이 난소절제에 의하여 유발된 흰쥐의 뼈 기질 회복 또는 손실 지연에 효과가 있다고 할 수 있을 것이다.

• 한국자원식물학회지
• /
• 제1권1호
• /
• pp.42-47
• /
• 1988

생약식물로서 중약한 위치를 점하고 있는 구기자의 전지정도에 따른 품질 및 수량반응을 구명하기 위하여 1982 ~ '83년 2개년에 걸쳐 "진도재래"를 공시시험한 결과는 다음과 같다. 1. 구기자의 개화반응은 맹모 - 황엽 - 낙엽 - 개화의 일련의 단계를 거쳐 개화되었고, 2. 무전지구에 비해 전지구가 간장이 길고 그에 따른 절수도 많았으며 총 유효분지수가 많아 수량에 영향을 주었다. 3. 무전지구에서는 과정이 짧아 과실이 잘고 전지구는 과정이 길고 굵은 충실한 과실이 생산되어 상품가치가 높았다. 4. 10 a 당 수량은 무전지 방치구(76kg)에 비하여 지표면 전지구와 지표면 10 cm 전지구에서 38 ~ 39%의 증수효과가 있어 구기자는 모년 무전하여 재배하는 것이 바람직하다고 사료된다. 5. 구기자 수확회수별 수량은 2회 수확으로 생산량의 85%를 수확할 수 있어 수학노력 절감이 앞으로의 과제라 하겠다.

• Preventive Nutrition and Food Science
• /
• 제11권4호
• /
• pp.328-332
• /
• 2006

Baikseolgi was made with Kugija powder with the intention to take advantage of its functional properties. Appropriate amount of Kugija powder (0$\sim$8%) was mixed with rice flour, sugar, salt, and water and then, steamed for predetermined time and their physicochemical and sensory properties were measured. pH decreased significantly while titratable acidity increased significantly with the addition of Kugija (p<0.05). The addition of Kugija powder did not significantly affect the moisture content (p>0.05) although it appeared to decrease with the higher amount of Kugija. Lightness (L-value) decreased significantly with the addition of Kugija (p<0.05), indicating that the color of Baikseolgi became dark as also indicated by the visual observation. Redness (a-value) and yellowness (b-value), on the other hand, increased significantly as the amount of Kugija increased in the sample (p<0.05). Both hardness and firmness decreased significantly with the addition of Kugija (p<0.05). Eight percentage Kugija Baikseolgi was significantly stronger in Kugija flavor (7.9), chewiness (6.1), sweetness (5.0), and yellowness (8.2) attributes than other samples, whereas control was significantly higher in mouthfeel (6.0) and hardness (6.0) attributes than others (p<0.05). Finally, consumer test indicated that 4% Kugija sample received the highest score in appearance (8.13) and overall acceptability (8.07) attributes.

• 한국약용작물학회:학술대회논문집
• /
• 한국약용작물학회 2012년도 심포지엄 및 춘계학술발표회
• /
• pp.43-44
• /
• 2012

• 혜화의학회지
• /
• 제25권1호
• /
• pp.133-144
• /
• 2016

Objectives : This study was aimed to investigate the effect of ACLL extract (Artemisia capillaris Thunb, Curcuma longa L, Lycium chinense, Ligustrum lucidum Aiton complex extract) in a dyslipidemia animal model induced by a high-fat diet. Methods : ACLL extracts was administered at 200 mg/kg, and 400 mg/kg to Apoe mice, where arthritis was induced by high cholesterol diet. After mice were treated with GO for 4 weeks, we measured AST, ALT, BUN and lipid levels (total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride), atherogenic index(AI) and cardiac risk factor(CRF). Results : Serum AST, ALT, creatinine, BUN levels were not changed by ACLL extract do not show any toxic effects. Also, ACLL extract groups were found to have atherogenic Index and cardiac risk factor as well as lipid metabolism improvement (total cholesterol, LDL cholesterol and triglyceride decrease). Conclusions : We suggest that ACLL extract may have the control effects of Dyslipidemia by improving lipid metabolism.

• Mycobiology
• /
• 제39권3호
• /
• pp.164-169
• /
• 2011

Spent mushroom compost (SMC) of Pleurotus pulmonarius (an edible fungus) was used as soil conditioner for the improvement of growth of four common Nigerian vegetables (Abelmoschus esculentus, Lycopersicum esculentum, Capsicum annum and Capsicum chinense). The results of these investigations showed that the vegetables responded well to the SMC treatment. Each of them attained its best growth and gave the highest number of flowers and fruits when planted on 6 kg of depleted garden soil supplemented with 600 g of SMC. The control experiment that has the seedlings of the vegetables planted on 6 kg of depleted garden soil only, without the application of SMC, showed stunted and poor growth, with few or no flower and fruit production. A. esculentus was the best utilizer of iron utilizing 118.0 mg/kg in the SMC used. Similarly; this vegetable utilized 1.48 mg/kg of nitrogen in the SMC. The highest height in each vegetable was attained with 6 kg of depleted garden soil supplemented with 600 g of SMC. At 9 wk, A. esculentus has the mean height of 85.0 cm while these values significantly increased to 100.00 cm at 14 wk ($p{\leq}0.05$). At 9 wk, L. esculentum has the highest mean height of 65.00 cm which increased to 71.00 cm after 14 wk. It was also observed that A. esculentus has the highest mean number of fruits (9.00), followed in order by C. chinense (8.00) and L. esculentum (7.00) ($p{\leq}0.05$) while, C. annum produced the least mean number of fruits (5.00). No fruits production was seen in the control experiments. The results of these findings were discussed in relation to the usage of SMC as possible organic fertilizer for the improvement of growth of vegetables in Nigeria.