• 식물분류학회지
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• 제50권1호
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• pp.1-7
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• 2020

Cynanchum L. in the subfamily Asclepiadoideae (Apocynaceae) includes four recognized species on the Korean peninsula, two of which are native to South Korea. However, the species ranges in South Korea are poorly defined. During a field survey, we discovered C. thesioides, previously unrecorded in South Korea, in Gimpo-si, Gyeonggi-do, and found an additional population of C. chinense, for which only one population has been reported in South Korea. The two taxa are considered rare species with extremely restricted distributions in South Korea, especially C. thesioides. We provide fundamental information, including descriptions, images of the habitats and morphological characters, and a taxonomic key for identification and assessments of the conservation status of Cynanchum species in Korea.

• 식물조직배양학회지
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• 제28권6호
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• pp.329-333
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• 2001

배지, 절편체의 종류 그리고 생장조절제가 약용작물인 구기자나무 (Lycium chinese Mill. 'Cheongyang')의 캘러스 유도 및 신초 분화조건에 미치는 영향을 조사하였다. 배지 종류별 기관분화는 신초 분화율, 신초 발생수 및 생체중이 MS, B5, WPM 배지 순으로 양호하였다. 구기자나무의 캘러스 유도는 절간 절편체보다 잎 절편체가 좋았고, 캘러스는 0.5mg/L NAA와 0.2 mg/L BA가 첨가된 MS배지에서 효율적으로 유도되었다. 신초 분화에 관여하는 cytokinin으로는 TDZ보다는 BA가 좋았으며 BA 단일처리보다는 NAA와의 혼용처리에서 더 양호하였다. 신초분화는 0.01 mg/L NAA와 0.2 mg/L BA가 첨가된 MS 배지에서 가장 좋았다

• 한방안이비인후피부과학회지
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• 제15권1호
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• pp.1-30
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• 2002

This experimental study was done to research effects of a number of extract on the anti-allergic effect. The results were obtained as follows: 1. In effect of herb-extract on compound $\frac{48}{80}$-mediated histamine release from Evans blue skin assay, Isatis Japonica Miquel, Dictamnus dasycarpus Turcz, Spirodela polyrhiza, Cimicifuga heracleifolia, Bupleurum chinense, Magnolia liliflora, Forsythia koreana, Aster tataricus L., Xanthium strumarium L.(MtOH), Trichosanthes kirilowii, Phellodendron amurense Rupr, Schizonepeta tenuifolia Var, Betula platyphylla show considerable visible anti-allergic effect. In the result of quantification of histamine induced compound $\frac{48}{80}$, Spirodela polyrhiza, Isatis Japonica Miquel, Trichosanthes kirilowii, Bupleurum chinense, Forsythia koreana inhibit histamine release effectively. 2. In effect of Herb-Extract on compound $\frac{48}{80}$-mediated histamine release from RPMC, Spirodcla polyrhiza, Cimicifuga heracleifolia inhibit histamine release effectively. 3. In effect of Herb-Extract on anti-DNP IgE-mediated histamine release from Evansblue skin assay. Spirodela polyrhiza, Cimicifuga heracleifolia(0.1mg/ml). Forsythia koreana, Aster tataricus L., Xanthium strumarium L.(0.1mg/ml), Trichosanthes kirilowii(0.1mg/ml) show considerable visible anti-allergic effect. In the result of quantification of histamine induced anti-DNP IgE, Spiradela polyrhiza, Isatis Japonica Miquel, Trichosanthes kirilowii, Bupleurum chinense, Forsythia koreana inhibit histamine release effectively 4. In the result of genetic manifestative inhibition about the human mast cell-1(HMC-1), Cimicifuga heracleifolia has considerable effect in IL-4 in IL-5, and Tussilage farfara L. has in IL-4. According to the above results, it is suggested that several Herb-Extract have anti-allergic effect.

• 생약학회지
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• 제51권2호
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• pp.93-99
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• 2020

This study was performed to find anti-inflammatory or antitumor compounds from the polar fraction obtained from the extract of Clinopodium chinense var. shibetchense (H. Lev) Koidz (Labiatae). Chromatography of the BuOH fraction yielded two flavonoid glycosides (compounds 1 and 2) and two saponins (compounds 3 and 4). On the basis of spectroscopic data, compounds 1 and 2 were identified to be ponciretin 7-O-α-L-rhamnopyranosyl-(1→6)-α-D-glucopyranoside (neoponcirin) and naringenin 7-O-α-L-rhamnopyranosyl-(1→6)-α-D-glucopyranoside (isonaringin). Compounds 3 and 4 were identified to be 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranosyl}-saikogenin F (buddlejasaponin IV) and 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranosyl}-21β-hydroxysaikogenin F (clinoposaponin XV). In addition, ursolic acid (5) was isolated and identified from the CHCl₃ fraction. Inducible nitric oxide synthase (iNOS) assay and sulforhodamine B (SRB) assay were performed to lead a potential anti-inflammatory or anti-tumor compounds from C. chinense var. shibetchense. Of the four compounds (1 - 4), compound 3 considerably inhibited cancer cell growth and NO production (IC₅₀s, 5.59 μM in iNOS assay and 6.62 - 14.88 μM in SRB assay).

• 한국작물학회지
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• 제43권3호
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• pp.189-193
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• 1998

Volatile components were extracted from leaves of two Boxthorn (Lycium chinense M.) cultivars by using simultaneous distillation and extraction, analyzed by gas chromatography-mass spectrometry. Seventy components were identified : 13 acids, 15 alcohols, 18 hydrocarbons, 13 carbonyls, three esters, three ionones, and five others. The principal volatile components (and their peak area percentage) were n-pentanol (11.2～30.2%), phytol (14.5～28.3%), hexadecanoic acid (13.5～17.1%) 2,3-dihydrobenzofuran (1.5～4.2%), benzyl alcohol (1.9-4.8%), phenylacetaldehyde (1.8～3.2%), and octadecadienoic acid (1.7～10.7%). Fresh leaves showed much higher peak area than that of dried leaf in n-pentanol, n-hexanol, cis-2-penten-l-ol, cis-3-hexen-l-ol, benzyl alcohol, and $\beta$-phenylethyl alcohol, while dried leaves showed much higher content than that of fresh leaves in 9-hydroxytheaspran A, octadecanoic acid and octadecadienic acid.

• 식물조직배양학회지
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• 제22권6호
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• pp.329-334
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• 1995

효과적인 형질전환 시스템을 이용하여 왜화유전자인 rolC 유전자를 구기자나무로의 형질전환 시스템을 확립하였다. 침으로 자극된 엽절편을 2.0 mg/L zeatin이 함유된 3/2 MS배지에 배양하였을 때 엽표면으로부터 줄기재분화가 되었다. 그러나 여러 농도의 kanamycin sulfate와 2.0 mg/L Aeatin이 함유된 배지에 엽절편을 배양하였을 때는 kanamycin sulfate의 농도가 증가할수록 줄기 유발수가 감소하는 것을 볼 수 있었으며, 적정선발농도는 10 mg/L이었다. 엽절편은 공동배양 시간에 따라서 생존율과 줄기재분화에 매우 큰 영향을 미쳤다. 엽절편의 생존율은 dipping할 경우가 가장 좋았으며, 배양시간이 길수록 엽절편의 백색화가 관찰되었고, 생존율이 급격히 감소하는 것을 볼 수 있었다. 줄기재분확에 가장 적합한 공동배양 시간은 24시간으로 나타났다. 공시균주와 24시간 동안 공동배양한 엽절편을 10mg/L의 kanamycin sulfate와 2.0 mg/L zeatin이 함유된 줄기 유도배지에 배양한 결과, 105개의 엽절편 중 80개의 엽절편이 생존하였으며, 그 중에서 15개의 재분화된 줄기를 얻었다. 재분화된 줄기들은 형질전환여부를 판명하기 위해 1차적으로 10 mg/L kanamycin sulfate가 함유된 배지에 옮겨 4 주간 배양한 결과, 항생제에 대해서 저항성을 가진 5개의 식물체를 선발할 수 있었다. 2차적으로 rolC유전자와 NPTII 유전자 도입의 유무를 검증하기 위하여 Southern 분석을 행한 결과, 구기자의 형질전환 식물체에서 rolC 유전자 probe의 coding sequence와 동일한 것으로 생각되는 1kb위치와 NPTII probe의 coding sequence와 동일한 것으로 생각되는 2.6kb 위치에서 각각의 밴드를 확인할 수 있었다.

• 농업과학연구
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• 제41권4호
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• pp.341-350
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• 2014

The occurrence of insect pests of Lycium chinense was monitored at 4 organic farms that was cultivated with environment-friendly management at Cheongyang, Chungnam from May, 2012 to September, 2014. Total 4 orders and 18 species occurred in the L. chinense. Among them, 4 major pests and Lepidopteran and Hemipteran insect pests were investigated the occurrence pattern. During the budding, the population density of Myzus persicae was very high in late-May and early-June, and the injury by Lema decempunctata was increased from mid-June. The injuries by Henosepilachna vigintioctomaculata and Hedma lycia sp. were continued throughout the growing season. From the fruit held in late-July, hemipteran insects including Plautia stali and Halyomorpba balys were sucking the fruit, and their injury to L. chinense were gradually increased. In the case of Eriophys macrodonis densities to increase gradually from early July. The population density of E. macrodonis in 2014 was rapidly increased more than 5 times compared with 2012 and 2013. It was supposed that the initial control of mites or the removing of over-wintering density in the past year might be neglected. However, there is a need to more review the factors for the mass occurs.

• 농업과학연구
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• 제41권4호
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• pp.361-367
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• 2014

Host plants of the Henosepilachna vigintioctomaculata are known as Solanaceae plants such as potato, tomato, eggplant and Lycium chinense. Henosepilachna vigintioctomaculata (Motschulsky) cause continuous damage from early June to mid-August in leaves of L. chinense under environmental-friendly management in Cheongyang, Chungnam. The developmental period of egg and larva of H. vigintioctomaculata were investigated. H. vigintioctomaculata were reared on L. chinense at several temperatures, for example, 15, 20, 25 and $30^{\circ}C$ in the laboratory. In addition, the control effect of 5 eco-friendly agricultural materials were examined. As results, hatching rate is the highest to 91% at $25^{\circ}C$. Egg periods were 10.8, 7.7, 5.1 and 3.7 days at each tested temperature, respectively. And the total developmental periods from egg to adult were 42.7, 26.3, 18.4 and 19.4 days at each tested temperature, respectively. Otherwise, their eclosion rates from pupa to adult were showed 58%, 77%, 85%, 65% at each tested temperature, respectively. As a result of the control effect, both adults and larvae of H. vigintioctomaculata were shown a high mortality to mixture with extract of Sophora flavescens, Chenopodium ambrosioides and Melia azedarach. Additional, larvae is shown high mortality to extract of Azadirachta indica and Cymbopogon nardus. Thus H. vigintioctomaculata might be controled by using plant extract of eco-friendly agricultural materials in due season.

• Journal of Crop Science and Biotechnology
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• 제10권4호
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• pp.277-280
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• 2007

Pepper fruit anthracnose, caused by Colletotrichum acutatum, results in serious yield loss and affects crop quality in many Asian countries, making it a disease of economic consequence. A source resistant to C. acutatum was identified by the AVRDC within the line Capsicum chinense Jacq. PBC932. The resistant breeding line C. annuum AR is the $BC_3F_6$ generation derived from C. chinense Jacq. PBC932. The inheritance of resistance to C. acutatum was analyzed in segregating populations derived from the two crosses HN 11$\times$AR and Daepoong-cho$\times$AR. Detached mature green fruits were inoculated using microinjection method. The disease response was evaluated as the disease incidence at 7 DAI. The segregation ratios of resistance and susceptibility to C. acutatum in the $F_2$ and $BC_R$ populations derived from the two crosses fit significantly to a 1:3 Mendelian model. This indicates that the resistance of AR to C. acutatum is controlled by a single recessive gene.

• 한국자원식물학회지
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• 제20권3호
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• pp.251-254
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• 2007

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.