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Bacterial Node Soft Rot of Pepper (Capsicum annuum L.) Caused by Erwinia carotovora subsp. carotovora (Erwinia carotovora subsp. carotovora에 의한 고추 마디 무름병)

  • 정기채;임진우;김승한;임양숙;김종완
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.741-743
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    • 1998
  • A bacterial disease of pepper (Capsicum annuum L.) that rooted the stem nodes to black was found in pepper plants which cultivated in plastic house at Chungdo, Kyungpook, Korea in March, 1998. Bacterial isolates derived from the diseased peppers were pathogenic to potato, eggplant and Chinese cabbage but, was not pathogenic to chrysanthemum by artificial inoculation. On the basis of bacteriological characteristics and pathogenicity test on host plants, the causal organism of the node soft rot of pepper is identified as Erwinia carotovora subsp. carotovora and the name of disease is proposed as bacterial node soft rot of pepper.

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Data Transfer Efficiency Analysis Considering Mobility in WiBro System (WiBro 시스템에서 이동성을 고려한 데이터 전송효율 분석)

  • Lim Seog-Ku
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.6 no.5
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    • pp.446-452
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    • 2005
  • As the name means, the WiBro is mobile service so that radio internet connection may be possible with high transmission speed using various terminal. Because WiBro is choosing suitable All-lP network architecture in internet service offer, structure of network is simple and the various supplementary service offer is available. This paper presented mobility model of terminal and proposed efficient way to improve existent way that transfer efficiency drops by data padding and analyze the performance by simulation.

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Taxonomic notes on the genus Alsidium C. Agardh, including the merging of Bryothamnion Kützing (Rhodomelaceae)

  • Garcia-Soto, Gabriela;Lopez-Bautista, Juan
    • ALGAE
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    • v.33 no.3
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    • pp.215-229
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    • 2018
  • In this study, the phylogenetic relationships among the genera Alsidium C. Agardh and Bryothamnion $K{\ddot{u}}tzing$ were investigated. Phylogenetic analyses using the plastid-encoded markers rbcL, psbA, and the mitochondrial barcode region (COI-5P) resolved a well-supported clade that included the species Alsidium corallinum, Bryothamnion seaforthii, and B. triquetrum. Our results indicated that taxonomic recognition of the genus Bryothamnion is not supported and two species of Bryothamnion are reallocated to Alsidium. A reexamination of the morphological definition of Alsidium is provided with an updated diagnosis of the genus and a morphology-based comparison of species that are currently circumscribed under this generic name. Furthermore, we reviewed morphological differences and similarities between Alsidium and the genus Digenea, both belonging to the tribe Alsidieae, discussing the most relevant morphological characters.

A re-examination of Gobiid Fish, Luciogobius guttatus, with First Record of L. martellii in Korea (한국산 망둑어과 (Gobiidae) 미끈망둑 (Luciogobius guttatus)의 재검토 및 첫기록종, L. martellii)

  • Min-Soo Kim;Seong-Ho Choi;Jae-Goo Kim;Youn Choi
    • Korean Journal of Ichthyology
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    • v.35 no.4
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    • pp.378-383
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    • 2023
  • A re-examination of Luciogobius guttatus was conducted using 84 samples, which lives on all coastal waters of Korea. As a result, 41 specimens (31.3~54.4 mm SL) collected from the west and south coasts were classified as L. martellii. The morphological features of L. martellii as follows: number of abdominal vertebrae 16; total number of vertebrae 35~37; dorsal fin base length longer than upper caudal peduncle length or equal; one free ray on its upper pectoral fin; and edge of tail fin white. Among these, the most prominent morphological difference in L. guttatus is comparing the dorsal fin base length and upper caudal peduncle length, which is also well observed in syntypes of L. martellii. We proposed a new Korean name, "Geom-jeong-mi-kken-mang-duk" referring to their color.

The Relaxant Effect of Propofol on Isolated Rat Intrapulmonary Arteries

  • Zhang, Guangyan;Cui, Jianxiu;Chen, Yijing;Ma, Jue
    • The Korean Journal of Physiology and Pharmacology
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    • v.18 no.5
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    • pp.377-381
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    • 2014
  • Propofol is a widely used anesthetic. Many studies have shown that propofol has direct effects on blood vessels, but the precise mechanism is not fully understood. Secondary intrapulmonary artery rings from male rats were prepared and mounted in a Multi Myograph System. The following constrictors were used to induce contractions in isolated artery rings: high $K^+$ solution (60 mmol/L); U46619 solution (100 nmol/L); 5-hydroxytryptamine (5-HT; $3{\mu}mol/L$); or phenylephrine (Phe; $1{\mu}mol/L$). The relaxation effects of propofol were tested on high $K^+$ or U46619 precontracted rings. Propofol also was added to induce relaxation of rings preconstricted by U46619 after pretreatment with the nitric oxide synthase inhibitor $N^G$-nitro-L-arginine methyl ester (L-NAME). The effects of propofol on $Ca^{2+}$ influx via the L-type $Ca^{2+}$ channels were evaluated by examining contraction-dependent responses to $CaCl_2$ in the absence or presence of propofol (10 to $300{\mu}mol/L$). High $K^+$ solution and U46619 induced remarkable contractions of the rings, whereas contractions induced by 5-HT and Phe were weak. Propofol induced dose-dependent relaxation of artery rings precontracted by the high $K^+$ solution. Propofol also induced relaxation of rings precontracted by U46619 in an endothelium-independent way. Propofol at different concentrations significantly inhibited the $Ca^{2+}$-induced contractions of pulmonary rings exposed to high $K^+$-containing and $Ca^{2+}$-free solution in a dose-dependent manner. Propofol relaxed vessels precontracted by the high $K^+$ solution and U46619 in an endothelium-independent way. The mechanism for this effect may involve inhibition of calcium influx through voltage-operated calcium channels (VOCCs) and receptor-operated calcium channels (ROCCs).

Palynotaxonomic Study on the Korean Pyrolaceae (한국산 노루발과(Pyrolaceae)의 화분분류학적 연구)

  • Chung, Gyu-Young;Nam, Gi-Hum;Park, Myung-Soon
    • Korean Journal of Plant Resources
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    • v.20 no.2
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    • pp.193-200
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    • 2007
  • Pollen grains of seven taxa on the family Pyrolaceae, known to be distributed in Korea (Pyrola dahurica(Andreses) Kom., P. incarnata (DC.) Fisch. ex Kom., P. japonica Klenze ex Alefeld, P. minor L., P. renifolia Max., Orthilia secunda (L.) House, Chimaphila japonica Miq.), were examined by the light and the scanning electron microscope in order to evaluate their taxonomic significances. The pollen units of genus Pyrola, Orthilia, Chimaphila were tetrad, monad, polyad respectively. This characteristics was good character for delimiting the genus, and Orthilia secunda (L.) House was more proper scientific name than Pyrola secunda L. based upon this character. Pollen size, tricolporate length, surface sculpturing pattern were different slightly among the treated taxa, and surface sculpturing patterns of Pyrola dahurica, P. incarnate, P. renifolia, Chimaphiza japonica were different from previous reports. Evolutionary trends of pollen grains in Pyrolaceae were inferred from the pollen units and aperture shapes.

Identification of Marker Nucleotides for the Molecular Authentication of Araliae Continentalis Radix Based on the Analysis of Universal DNA Barcode, matK and rbcL, Sequences (범용성 DNA 바코드(matK, rbcL) 분석을 통한 독활(獨活) 유전자 감별용 Marker Nucleotide 발굴)

  • Kim, Wook Jin;Yang, Sungyu;Choi, Goya;Moon, Byeong Cheol
    • The Korea Journal of Herbology
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    • v.31 no.5
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    • pp.15-23
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    • 2016
  • Objectives : Araliae Continentalis Radix and Angelicae Pubescentis Radix have been used as the same medicinal name Korean and Chinese traditional medicines, respectively. The authentic Araliae Continentalis Radix is described only the root of Aralia continentalis in the Korean Pharmarcopoeia. However, the dried root of Angelica biserrata, Levisticum officinale, or Heracleum moellendorffii also has been distributed adulterants of Araliae Continentalis Radix. To develop a reliable method for identifying Araliae Continentalis Radix from adulterants, we carried out the analyses of universal DNA barcode sequences.Methods : Four plants species were collected from different habitate and nucleotide sequences of matK and rbcL were analyzed. The species-specific sequences and phylogenetic relationship were estimated using entire sequences of two DNA barcodes, respectively.Results : In comparative analysis of matK sequences, we were identified 104 positions of marker nucleotide for Ar. continentalis, 3 for An. biserrata, 4 for L. officinale and 8 for H. moellendorffii enough to distinguish individual species, respectively. Furthermore, we obtained marker nucleotides in rbcL at 42 positions for Ar. continentalis, 5 for An. biserrata and 2 for H. moellendorffii, but not for L. officinale. The phylogenetic tree of matK and rbcL were showed that all samples were clustered into four groups constituting homogeneous clades within the species.Conclusions : We confirmed that species-specific marker nucleotides of matK sequence provides distinct genetic information enough to identify four species. Therefore, we suggest that matK gene is useful DNA barcode for discriminating authentic Araliae Continentalis Radix from inauthentic adulterants.

Magnesium-induced Relaxation in Rat Aorta (Magnesium에 의한 흰쥐 대동맥 이완)

  • Oh, Sung-suck;Lee, Sang-woo;Kang, Hyung-sub;Kim, Jin-shang
    • Korean Journal of Veterinary Research
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    • v.43 no.3
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    • pp.373-382
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    • 2003
  • Magnesium ion ($Mg^{2+}$) is a vasodilator, but little is known about its mechanism of action on vascular system. In vitro, extracellular magnesium sulfate ($MgSO_4$) produced relaxation in phenylephrine (PE) or high KCl-precontracted isolated rat thorocic aorta with (+E) or without (-E) endothelium in a concentration-dependent manner. The $MgSO_4$-induced relaxations were not affected by removal of the endothelium. Pretreatment of +E or -E aortic rings with nitric oxide synthase (NOS) inhibitors ($20{\mu}M$ L-NNA, $100{\mu}M$ L-NAME, $1{\mu}M$ dexamethasone and $400{\mu}M$ aminoguanidine), cyclooxygenase inhibitor ($10{\mu}M$ indomethacin), guanylate cyclase inhibitors ($10{\mu}M$ ODQ and $30{\mu}M$ methylene blue) and $Ca^{2+}$ transport blocker ($10{\mu}M$ ryanodine) did not affect the relaxant effects of $MgSO_4$. $Ca^{2+}$ channel blockers ($0.3{\mu}M$ nifedipine and $0.5{\mu}M$ veropamil) completely decreased the relaxant effects of $MgSO_4$ in +E and -E aortic rings. However, in $Ca^{2+}$-free medium, $MgSO_4$-induced vasorelaxation was potentiated and this response was inhibited by nifedipine. Protein kinase C (PKC) inhibitors ($1.0{\mu}M$ staurosporine, $0.5{\mu}M$ tamoxifen and $0.1{\mu}M$ H7) or PLC inhibitor ($100{\mu}M$ NCDC) markedly decreased the relaxant effects of $MgSO_4$ in +E and -E aortic rings. In vivo, infusion of $MgSO_4$ elicited significant decreases in arterial blood pressure. After intravenous injection of nifedipine ($150{\mu}g/kg$) and NCDC (3 mg/kg), infusion of $MgSO_4$ inhibited the $MgSO_4$-lowered blood pressure markedly. However, after introvenous injection of saponin (15 mg/kg), L-NNA (3 mg/kg), L-NAME (5 mg/kg), indomethacin (2 mg/kg), methylene blue (15 mg/kg) and aminoguanidine (10 mg/kg) failed to inhibit it. These results suggest that endothelial NQ-cGMP or prostaglandin pathway is not involved in vasorelaxant or hypotensive action of $Mg^{2+}$ and that these effects are due to the inhibitory action of $Mg^{2+}$ on the $Ca^{2+}$ channel or PLC-PKC pathway, and are due to the competitive influx of $Mg^{2+}$ and $Ca^{2+}$ through the $Ca^{2+}$ channel.

A Study on the Pre-printed Clause of the Bill of Lading (선하증권 인쇄약관에 관한 연구)

  • Park, Sae-Woon
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.49
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    • pp.359-378
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    • 2011
  • UCP600 Article 20 (a) (v) states that contents of terms and conditions of carriage will not be examined and Article 34 states that a bank assumes no liability or responsibility for the general or particular conditions stipulated in a document. From this perspective, banks may seem to have no obligation to examine the pre-printed clause of B/L. However, ICC decided that no opinion could be given in relation to the issues surrounding B/Ls that contain delivery clauses. Accordingly, it is agreed by previous cases and some scholars that banks may refuse the B/Ls that contain delivery clauses which are not present in other B/Ls of the same goods and transport routes. Also, ICC published ICC Decision in July 2010 regarding on board notation. In this decision, if a B/L indicates a place of receipt that is different from the port of loading and there is an indication of a means of pre-carriage, then a dated on board notation will be required indicating the name of the vessel and the port of loading. Therefore, banks may,, in some cases, need to scrutinize the pre-printed clauses especially appearing in the front page of B/Ls.

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Enhancement of Ethanol Production via Hyper Thermal Acid Hydrolysis and Co-Fermentation Using Waste Seaweed from Gwangalli Beach, Busan, Korea

  • Sunwoo, In Yung;Nguyen, Trung Hau;Sukwong, Pailin;Jeong, Gwi-Teak;Kim, Sung-Koo
    • Journal of Microbiology and Biotechnology
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    • v.28 no.3
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    • pp.401-408
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    • 2018
  • The waste seaweed from Gwangalli beach, Busan, Korea was utilized as biomass for ethanol production. Sagassum fulvellum (brown seaweed, Mojaban in Korean name) comprised 72% of the biomass. The optimal hyper thermal acid hydrolysis conditions were obtained as 8% slurry contents, 138 mM sulfuric acid, and $160^{\circ}C$ of treatment temperature for 10 min with a low content of inhibitory compounds. To obtain more monosaccharides, enzymatic saccharification was carried out with Viscozyme L for 48 h. After pretreatment, 34 g/l of monosaccharides were obtained. Pichia stipitis and Pichia angophorae were selected as optimal co-fermentation yeasts to convert all of the monosaccharides in the hydrolysate to ethanol. Co-fermentation was carried out with various inoculum ratios of P. stipitis and P. angophorae. The maximum ethanol concentration of 16.0 g/l was produced using P. stipitis and P. angophorae in a 3:1 inoculum ratio, with an ethanol yield of 0.47 in 72 h. Ethanol fermentation using yeast co-culture may offer an efficient disposal method for waste seaweed while enhancing the utilization of monosaccharides and production of ethanol.