• Title/Summary/Keyword: L-Feed

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Effects of lactic acid bacteria inoculation in pre-harvesting period on fermentation and feed quality properties of alfalfa silage

  • Ertekin, Ibrahim;Kizilsimsek, Mustafa
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.2
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    • pp.245-253
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    • 2020
  • Objective: To develop the fermentation quality and chemical composition of alfalfa (Medicago sativa Lam.) silage, plants were inoculated with different lactic acid bacteria (LAB) strains at field 24 hours before harvest. Methods: The treatment groups were as follow: silage without additive as a control and inoculated with each strains of Lactobacillus brevis (LS-55-2-2), Leuconostoc citerum (L. citerum; L-70-6-1), Lactobacillus bifermentans (L. bifermentans; LS-65-2-1), Lactobacillus plantarum (L. plantarum; LS-3-3) and L. plantarum (LS-72-2). All the silages were stored at 25℃. Parameters such as pH, microorganism and volatile fatty acid contents, crude protein, neutral detergent fiber, acid detergent fiber, net gas, metabolizable energy, organic matter digestibility, dry matter intake and relative feed value were measured to determine fermentation quality, chemical compositions and relative feed value of alfalfa silages. Results: Significant differences were found among the control and treated groups in terms of pH and microorganism contents at all opening times and crude protein, net gas, metabolizable energy and organic matter digestibility of final silage. The pH values ranged from 4.70 to 5.52 for all treatments and control silage had the highest value of overall treatments at T75d silages. Volatile fatty acid of silages was not influenced significantly by inoculations. However, lactic acid content of L. bifermentans (LS-65-2-1) was higher than the other treatments. The highest metabolizable energy and organic matter digestibility were recorded from L. citerum (L-70-6-1) inoculation. In addition, no significant differences were found among treatments in terms of neutral detergent fiber, acid detergent fiber, dry matter intake and relative feed value. Conclusion: Among the treated LAB isolates, L. bifermentans came into prominence especially in terms of organic acid composition and quality characters of silages.

Performance Study of Membrane Capacitive Deionization Process Applied by Perfluoropolymer and Aminated Poly(ether imide) Ion Exchange Membranes (불소화고분자와 아민화된 폴리이서이미드 이온교환막을 적용한 축전식 탈염공정의 성능 연구)

  • Kim, Ji Seon;Jeong, Joo Hwan;Rhim, Ji Won
    • Membrane Journal
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    • v.25 no.1
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    • pp.60-66
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    • 2015
  • To investigate the performance of the composite carbon electrodes which the ion exchange polymers were directly casted onto porous carbon electrode surfaces, the adsorption/desorption experiments were carried out by varying the feed concentration, feed flow rate, and desorption voltages for the NaCl solution. When the feed concentration was 100 mg/L, the increase of the adsorption time led to the increase of the salt removal due to the increase of the residence time inside the cell while the increase of the feed flow rate from 15 mL/min to 23 mL/min gave the decrease of the salt removal efficiency, 12% because of the short residence time. When the feed concentration was 200 mg/L, the salt removal was shown 10~15% low because of the incomplete desorption within the desorption intervals.

Metamorphosis and survival rate of mud crab (Scylla olivacea) larvae fed with enriched live feed with Moringa oleifera leaves

  • Zainal Usman;Muhammad Yusri Karim;Zainuddin Zainuddin;Syafiuddin Syafiuddin;Khairun Nisaa
    • Fisheries and Aquatic Sciences
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    • v.27 no.7
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    • pp.456-467
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    • 2024
  • The objective of this research was to evaluate live-feed effect enriched with Moringa oleifera Leaves upon the rate of metamorphosis and survival from zoea to megalopa of Scylla olivacea larva. The study utilized a live feed consisting of Rotifer (Branchionus plicatilis) and Artemia (Artemia salina) that were supplemented with moringa leaf powder. The enrichment process involved soaking the feed in solutions containing 0, 50, 100, and 150 mg/L of the powder The levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were quantified following the enrichment of the live feed. A further phase involved conducting a feeding study using mud crab larvae. This experiment was conducted with three replications. Feeding mud crab larvae using rotifers starts from stage 1 of zoea to megalopa with a frequency of 2 times a day at a 30 ind/mL density, and using artemia nauplius was from the zoea 2 to megalopa with a frequency of feeding two times a day at a 5 ind/mL density. The results showed that moringa leaf powder could improve EPA and DHA more significant in Rotifer than in Artemia. Combined rotifer and Artemia nauplii enrichment with 100 mg/L of moringa leaf powder positively impacted the proximate mud crab. This treatment also implies that the metamorphosis of mud crab from zoea 1 stage to megalopa become faster than other treatment. The development from zoea 1 to megalopa stage takes around 16 days. The treatment that yielded the highest survival rate (SR) was feeding mud crab larvae with enhanced live feed containing 100 mg/L of moringa leaf powder. This study demonstrated that the incorporation of moringa leaf powder into live feed can enhance the physical condition of mud crab larvae. Furthermore, it suggests that this enrichment can accelerate the progression of developmental stages and enhance the SR of mud crab larvae when reared under controlled settings.

목초액의 수준별 급여 및 생균제와 혼합 급여가 육계의 생산성 및 혈액의 성상에 미치는 영향

  • 이흥룡;김상호;신원집;류경선
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2001.11a
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    • pp.61-63
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    • 2001
  • Two experiments were conducted to investigate the feeding influence of wood vinegar and mixed with probiotics on performance and physiological related of broiler chicks for five weeks. Diets contained 21.5%, 3,100kcal/kg and 19.0% CP, 3,100kcal/kg ME for starting and finishing period, respectively. In Expt 1, Four levels of wood vinegar(0, 0.05, 0.10, 0.20%) were fed into two hundred forty chicks with four replicates. In Expt 2, 0, 0.1% wood vinegar(W), 0, 0.1% lactobacillus casei(L) and bacillus subtilis (B) were applied to 2${\times}$2${\times}$2 facotrial design for eight treatments with four replicates. Weight gain, feed intake, feed cholesterol were detected at five weeks old in each experiment. In Expt 1, Weight gain of chicks fed 0.1% wood vinegar(WV) was increased upto 1645.8g, but was not significantly different. However, chicks fed 0.2% WV showed the highest gain and siginificantly different compared to that of control (P<0.05) for finishing period. Feed intake also showed similar tendency with weight gain. There were no statistical difference in feed conversion. Abdominal fat seemed to be decreased in WV supplemental groups, whereas ND antibody titer tended to increase in those treatments. In Expt 2, 0, 0.1% WV, lactobacillus, bacillus were combined with each other(W$\sub$0/L$\sub$0/B$\sub$0/, W$\sub$0/L$\sub$1/B$\sub$0/, W$\sub$0/L$\sub$0/B$\sub$1/, W$\sub$0/L$\sub$1/B$\sub$1/, W$\sub$1/L$\sub$0/B$\sub$0/, W$\sub$1/L$\sub$1/B$\sub$0/, W$\sub$1/L$\sub$1/ B$\sub$0/, W$\sub$1/L$\sub$0/B$\sub$1/, W$\sub$1/L$\sub$1/B$\sub$1). WV supplemental groups significantly improved feed conversion of all treatments(P<0.05). There were no interaction in weight gain and feed intake. Total cholesterol was signficantly decreased in WV plus lactobacillus and ladtobacillus plus bacillus treatments. Liver to body ratio(%) of lactobacillus plus WV significantly decreased compared to that of other treatments. The results of these experiments showed that wood vinegar tended to improve the performance of broiler chicks, but was not effect in its combination with lactobacillus or bacillus treatments.

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Effects of Supplemental Lactobacillus spp. on Performance and Cecum Microflora in Broiler (육계 생산성 및 맹장내 미생물에 대한 유산균의 첨가 효과)

  • 김상호;박수영;유동조;나재천;최철환;박용윤;이상진;류경선
    • Korean Journal of Poultry Science
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    • v.27 no.1
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    • pp.37-41
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    • 2000
  • This study was carried out to examine the effects of Lactobacillus spp. on performance and cecal microflora in broiler chicks. Eight diets consisting of basal diet(C), C+Lactobacillus crispatus BC7 (T2), C+Lactobacillus reuteri BC9 (T3), C+L.reuteri BC5+L.crispatus BC9 (T4), C+L. reuteri BC5 (T1)+L.reuteri BC9 (T5), C+L.crispatus BC7+L.reuteri BC9 (T6) and C+L.reuteri BC5+L.crispatus BC7+L.reuteri BC9 (T7), were fed to Ross male broiler chicks for 5 weeks. The level of supplemented Lactobacillus spp. was 107 cfu/g diet. Body weight and feed intake were measured every week, and cecal microfla was counted at 1 and 5 weeks for Lactobacillus and yeast. Body weight increased signigicantly in supplemental Lactobacillus treatments from 2 weeks of age (p<0.05). Chicks in T2, T5 and T5 of treatments were heavier than those of other treatments at 5 weeks of age(p<0.05). Viability was not different significantly. Feed intake and feed conversion also were not different, although feed conversion improved slightly in supplemental Lactobacillus treatments. Lactobacillus spp. of cecal content was increased in supplemental Lactobacillus treatments at 1 week, but no significance was found. The number of yeast in cecum was not different from that of supplemental Lactobacillus. In conclusion, Lactobacillus, L.crispatus BC7 and L.reuteri BC9 from broiler cecum could contribute to the increase in body weight with supplemented mono-or mixing of di-lactobacilli. The results indicate that Lactobacillus feeding can benefit to intestinal lactobacillus at early growing broiler.

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Effect of L- or DL-methionine Supplementation on Nitrogen Retention, Serum Amino Acid Concentrations and Blood Metabolites Profile in Starter Pigs

  • Tian, Q.Y.;Zeng, Z.K.;Zhang, Y.X.;Long, S.F.;Piao, X.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.5
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    • pp.689-694
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    • 2016
  • The objective of the current study was to evaluate the effect of supplementation of either L-methionine (L-Met) or DL-methionine (DL-Met) to diets of starter pigs on nitrogen (N) balance, metabolism, and serum amino acid profile. Eighteen crossbred ($Duroc{\times}Landrace{\times}Yorkshire$) barrows weighing $15.45{\pm}0.88kg$ were randomly allotted to 1 of 3 diets with 6 pigs per treatment. The diets included a basal diet (Met-deficient diet) containing 0.24% standardized ileal digestibility Met with all other essential nutrients meeting the pig's requirements. The other two diets were produced by supplementing the basal diet with 0.12% DL-Met or L-Met. The experiment lasted for 18 days, consisting of a 13-day adaptation period to the diets followed by a 5-day experimental period. Pigs were fed ad libitum and free access to water throughout the experiment. Results showed that the supplementation of either L-Met or DL-Met improved N retention, and serum methionine concentration, and decreased N excretion compared with basal diet (p<0.01). The N retention of pigs fed diets supplemented with the same inclusion levels of DL-Met or L-Met were not different (p>0.05). In conclusion, on equimolar basis DL-Met and L-Met are equally bioavailable as Met sources for starter pigs.

Performance Evaluation of a Closed-Loop Pressure Retarded Membrane Distillation for Brackish Water Desalination and Power Generation (기수담수화와 전력 생산을 위한 폐루프형 압력 지연식 막 증류 공정의 성능 평가)

  • Cho, Gyu Sang;Lee, Jun-Seo;Park, Kiho
    • Korean Chemical Engineering Research
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    • v.60 no.4
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    • pp.525-534
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    • 2022
  • In this study, we investigated the applicability and optimal operating strategy of a closed-loop pressure retarded membrane distillation (PRMD) for brackish water desalination. For effective operation with net power generation, high temperature of heat source over 90 ℃ and feed flow rate at 0.6 kg/s are recommended. At 3 g/L of feed concentration, the average permeate flux and net energy density showed 8.04 kg/m2/hr and 2.56 W/m2, respectively. The average permeate flux and net energy density were almost constant in the range of feed concentration from 1 to 3 g/L. Compared to the case with seawater feed, the PRMD with brackish water feed showed higher average permeate flux and net energy density. Thus, PRMD application using brackish water feed can be more effective than that using seawater feed in terms of power generation.

GPS/GLONASS Microstrip Active Antenna Apply to Curve Surface (곡면에 부착 가능한 GPS/GLONASS 마이크로스트립 능동 안테나)

  • Kim, Mi-Suk;Son, Seok-Bo;Joo, Hahn-Kie;Bae, Joon-Sung;Kim, Joon-O
    • Journal of the Korea Institute of Military Science and Technology
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    • v.14 no.1
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    • pp.1-8
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    • 2011
  • In this paper, dual-feed circularly polarized microstrip patch antenna for GPS L1, GPS L2, GLONASS L1 signal was fabricated by using stacked patch. It was fed by dual coaxial probe on the patch at 50ohm impedance, and was simulated to resonate at GPS L1, GPS L2, GLONASS L1. To realize characteristics of right hand circular polarization using dual-feed stacked patch antenna and hybrid coupler for $90^{\circ}$ phase difference. Output of hybrid coupler was contacted input of Low Noise Amplifier(LNA). The LNA using dual band pass filter was designed and fabricated. The measured results of the implemented antenna is VSWR < 1.5 : 1 and the gain of 32dB(Zenith) over at GPS L1, L2, GLONASS L1.

Fed-Batch Sorbose Fermentation Using Pulse and Multiple Feeding Strategies for Productivity Improvement

  • Giridhar, R.;Srivastava, A.K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.5
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    • pp.340-344
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    • 2000
  • Microbial oxidation of D-sorbitol to L-sorbose by Acetobacter suboxydans is of commercial importance since it is the only biochemical process in vitamin C synthesis. The main bottleneck in the batch oxidation of sorbitol to sorbose is that the process is severely inhibited by sorbitol. Suitable fed-batch fermentation designs can eliminate the inherent substrate inhibition and improve sorbose productivity. Fed-batch sorbose fermentations were conducted by using two nutrient feeding strategies. For fed-batch fermentation with pulse feeding, highly concentrated sorbitor (600g/L) along with other nutrients were fed intermittently in four pulses of 0.5 liter in response to the increased DO signal. The fed-batch fermentation was over in 24h with a sorbose productivity of 13.40g/L/h and a final sorbose concentration of 320.48g/L. On the other hand, in fed-batch fermentation with multiple feeds, two pulse feeds of 0.5 liter nutrient medium containing 600g/L sorbitol was followed by the addition of 1.5 liter nutrient medium containing 600g/L sorbitol at a constant feed rate of 0.36L/h till the full working capacity of the reactor. The fermentation was completed in 24h with an enhanced sorbose productivity of 15.09g/L/h and a sorbose concentration of 332.60g/L. The sorbose concentration and productivity obtained by multiple feeding of nutrients was found to be higher than that obtained by pulse feeding and was therefore a better strategy for fed-batch sorbose fermentation.

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Feed Restriction and Compensatory Growth in Guzerá Females

  • Neto, S. Gonzaga;Bezerra, L.R.;Medeiros, A.N.;Ferreira, M.A.;Filho, E.C. Pimenta;Candido, E.P.;Oliveira, R.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.6
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    • pp.791-799
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    • 2011
  • This study examined the effect of restricting feed intake and the subsequent compensatory growth in Guzera females. Eighteen animals with an initial age of 21 months and a mean weight of 268.17 kg were placed in three groups according to the alimentary regime: feed ad libitum; feed restricted to 20% dry matter; and feed restricted to 40% dry matter. In the restricted feed phase, the dry mater intake decreased as the restriction levels increased, influencing the reduction in intake of other nutrients. In the realimentation phase, the 40% restricted feed group ingested more dry matter (% BW) and crude protein ($weight^{0.75}$) than the group fed ad libitum (p<0.001). The serum nutrient concentrations were inversely proportional (p<0.001) to the restriction level, and there was no difference (p>0.001) in the realimentation phase. In the restricted feed phase, the final live weight decreased (p<0.05) as the restriction level increased. For the daily mean weight gain in the control group, there was no difference (p>0.05) compared to the animals with 20% feed restriction, but this was higher than in the group with 40% feed restriction. In the re-alimentation phase, the group with 40% feed restriction achieved higher weight gain rates, which was different from the control and 20% restriction groups. In both phases, the animals in the group with 40% feed restriction presented better feed conversion which was different (p<0.05) from the control group. In the feed restriction phase, it was observed that the intake of N, nitrogen excreted in feces and urine, nitrogen balance and nitrogen retention decreased (p<0.05) with the restriction level. None of the variables were influenced in the re-alimentation phase. These results show that feed restriction by 40% can be adopted as a nutritional management practice.