• Plant Biotechnology Reports
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• 제2권2호
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• pp.133-136
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• 2008

Cell cultures of Commiphora wightii (Arnott.) Bhandari were grown in shake flasks and a bioreactor and an increase in guggulsterone accumulation up to $18{\mu}g\;l^{-1}$ was recorded in cells grown in the production medium containing a combination of sucrose:glucose (4% total), precursors (phenylalanine, pyruvic acid, xylose, and sodium acetate), morphactin, and 2iP. A yield of $10g\;l^{-1}$ biomass and ${\sim}200{\mu}g\;l^{-1}$ guggulsterone was recorded in a 3-l flask and in a 2-l stirred tank bioreactor compared with 6.6 g biomass and $67{\mu}g\;l^{-1}$ guggulsterone in 250-ml flasks. Increased vessel size was correlated with increased biomass and guggulsterone accumulation. 2iP alone was not effective for biomass and guggulsterone accumulation in cell cultures of C. wightii.

• 한국조리학회지
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• 제23권4호
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• pp.74-80
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• 2017

This study was to investigate the physiological activity of cookies with Sanguisorba officinalis L. powder. Our results showed that the pH of the cookies ranged from 6.34 to 6.29, and the density ranged from 4.12 to 4.40. Increasing the amount of Sanguisorba officinalis L. powder in the cookies decreased the spread factor of the cookies and tended to decrease lightness (L) in the Hunter color value with yellowness (b) and redness (a) increased. For the textural characteristics, the addition of Sanguisorba officinalis L. powder increased hardness. The sensory evaluation showed that the cookies with Sanguisorba officinalis L. powder were preferred more than those without in color, odor, texture, taste and overall preference. Based on the results above, the cookies that contained 3% of Sanguisorba officinalis L. powder presented the best quality.

• Bulletin of the Korean Chemical Society
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• 제5권6호
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• pp.215-218
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• 1984

A series of S-(2,2-diethoxycarbonyl-1-phenylethyl)-L-cysteine derivatives (10a-e) were synthesized from the reaction of $\beta$$\beta$-diethoxycarbonylstyrene with L-cysteine in 1:1 aqueous methanol. Thus, S-(2,2-diethoxycarbonyl-1-phenylethyl)-L-cysteine( 10a), S-[2,2-diethoxycarbonyl-1-(3',4'-methylendioxy)ph enylethyl]-L-cysteine (10b), S-[2,2-diethoxycarbonyl-1-(3',4',5'-trimethoxy)phe nylethyl]-L-cyseine (10c), S-[2,2-diethoxycarbonyl-1-(p-hydroxy)phenylethyl] -L-cysteine (10d), S-[2,2-diethoxycarbonyl-1-(p-methoxy)phenylethyl] -L-cysteine (10e) were obtained in moderate to excellent yields. The structure of the adducts was characterized by analytical and spectral data. The effects of pH upon the product yields were also briefly examined.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.984-988
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• 2003

The actinomycete strain KH-6l4 possessed strong antifungal activity, especially antagonistic to the rice blast fungus, Pyricularia oryzae. Diaminopimelic acid (DAP) type and morphological and physiological characteristics, examined by scanning electron microscopy (SEM), indicated that KH-614 belonged to the genus Streptomyces. Antifungal agent produced by this strain was found to be most active, when the strain was cultured in the presence of glucose, polypeptone, and yeast extract (PY) medium for 6 days at $27^{\circ}C$. Based on the spectral report data, MS and NMR, the antifungal agent was identified as cyclo(L-leucyl-L-prolyl). According to the antimicrobial activity test measured by minimal inhibitory concentration (MIC), the cyclo(1eu-pro) exhibited the activity against Candida albicans IAM 4905, Mucor ramannianus IAM6218, Rhizoctonia solani IFO 6218, Aspergilus fumigatus ATCC 42202, Glomerella cingulata IFO 9767, Trichophton mentagrophytes ATCC 18749, and Trichophyton rubrum ATCC 44766, the order of MIC values were 50, 12.5, 5, 50, 25, 5, $5\;\mu\textrm{g}/ml$, respectively. Specifically, cyclo(1eu-pro) was one of the most effective elements against Pyricularia oryzae IFO 5994 with the MIC value of $2.5\;\mu\textrm{g}/ml$, thus indicating that cyclo(leu-pro) is a potential antifungal agent.

• Environmental Engineering Research
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• 제23권2호
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• pp.216-222
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• 2018

The growth characteristics of Spirulina platensis were investigated using four photo-bioreactors with $CO_2$-mixed air flows. Each reactor was operated under a specific condition: 3% $CO_2$ at 50 mL/min, 3% $CO_2$ at 150 mL/min, 6% $CO_2$ at 50 mL/min, and 6% CO2 at 150 mL/min. The 3% $CO_2$ at 150 mL/min condition produced the highest algal growth rate, while the 6% $CO_2$ at 150 mL/min conditioned produced the lowest. The algal growth performance was suitably assessed by the linear growth curve rather than the exponential growth. The medium pH decreased from 9.5 to 8.7-8.8 (3% $CO_2$) and 8.4-8.5 (6% $CO_2$), of which trends were predicted only by the pH-carbonate equilibrium and the reaction kinetics between dissolved $CO_2$ and $HCO_3{^-}$. Based on the stoichiometry between the nutrient amounts and cell elements, it was predicted that depleted nitrogen (N) at the early stage of the cultivation would reduce the algal growth rates due to nutrient starvation. In this study, use of the photobioreactors capable of good light energy distribution, proper ranges of $CO_2$ in bubbles and medium pH facilitated production of high amounts of algal biomass despite N limitation.

• Applied Biological Chemistry
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• 제33권1호
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• pp.87-92
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• 1990

Ergosterol을 생산하기 위하여 Saccharomyces sake KBA No. 6가 사용되었으며 ergosterol축적에 관여하는 각종 요인들이 검토되었다. 가장 효율적인 무기 질소원은 ammonium chloride이었으며, C/N 비율이 200/l이었을 경우 3.5%의 ergosterol이 세포 내에 축적되었다. Tween 80 0.2 %와 potassium nitrite 0.1 %를 동시에 사용하였을 경우, ergosterol 함량(%) 과 총 ergosterol량(mg/L)은 각각 56 %와 45% 증가하였다. 최적조건에서 ergosterol 함량은 1.73%에서 5.3%로 증가하였으며, 총 ergosterol량은 65.2 mg/L에서 135.15 mg/L로 증가하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.380-383
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• 2000

불완전 곰팡이의 일종인 Aureobasidium pullulans ATCC 42023 균주를 이용한 pullulan의 최적조건 및 pH 조절에 따른 pullulan의 생성 그리고 균체형태와 고분자 pullulan 생산에 미치는 영향을 조사 하였다. 삼각플라스크 내에서 최대의 pullulan 생산은 초기 pH 6.5일때 11.98 g/l의 pullulan을 생산하였고, 회분식 발효에서는 산소 공급속도 0.5 vvm일때 15.16 g/l의 최대의 pullulan생산량을 나타내었다. pH조절에 따른 실험에서는 pH 4.5일때 13.31 g/l의 pullulan 생산량을 보였고, 이때 균체의 형태는 효모형과 균사형태의 성장을 하였다. 그러나 분자량 생성면에서는 pH 6.5일때 100만 이상의 고분자가 가장 많이 생산되었다.

• KSBB Journal
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• 제28권6호
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• pp.366-371
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• 2013

Ethanol productions were performed by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes using seaweed, Enteromorpha intestinalis (sea lettuce). Pretreatment conditions were optimized by the performing thermal acid hydrolysis and enzymatic hydrolysis for the increase of ethanol yield. The pretreatment by thermal acid hydrolysis was carried out with different sulfuric acid concentrations in the range of 25 mM to 75 mM $H_2SO_4$, pretreatment time from 30 to 90 minutes and solid contents of seaweed powder in the range of 10~16% (w/v). Optimal pretreatment conditions were determined as 75 mM $H_2SO_4$ and 13% (w/v) slurry at $121^{\circ}C$ for 60 min. For the further saccharification, enzymatic hydrolysis was performed by the addition of commercial enzymes, Celluclast 1.5 L and Viscozyme L, after the neutralization. A maximum reducing sugar concentration of 40.4 g/L was obtained with 73% of theoretical yield from total carbohydrate. The ethanol concentration of 8.6 g/L of SHF process and 7.6 g/L of SSF process were obtained by the yeast, Saccharomyces cerevisiae KCTC 1126, with the inoculation cell density of 0.2 g dcw/L.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.321-324
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• 2002

본 연구에서는 hGM-CSF를 생산해 배지 내로 분바하도록 유전자 조작된 Nicotiana tabacum 세포를 수성이상계 내에서 배양하여 in situ recovery를 시도하였다. 특히 식물세포 자체의 생상에 큰 영향을 주지 않으면서도 일반 배지에서 회수한 단백질에 비해 많은 양을 생산할 수 있는 수성이상계 시스템을 선정하였다. 6% (w/w) PEG 20,000과 10% (w/w) dextran 2.000,000 을 이용하는 경우 최대 세포 농도가 18.6 g/L 로, 일반배지를 사용하는 경우 (15.7 g/L)에 비해 저해가 없음을 확인하였다. 또한 목적 단백질인 hGM-CSF의 생산에 있어서도 위의 시스템의 경우 dextran-rich phase인 아랫 상으로 분배됨으로 인해 회수가 쉬울 것으로 확인되었으며 그 생산량 또한 일반배지에서의 생산량 (1.5 ng/mL)에 비해 크게 다르지 않음을 확인할 수 있었다. 따라서 hGM-CSF의 회수를 위한 in situ recovery 시스템에 있어서 수성이상계의 이용 가능성을 확인하였다.

• 공업화학
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• 제19권4호
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• pp.366-369
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• 2008

입자상 알루미늄 충전복극조의 성능을 파악할 목적으로 실험은 회분식과 연속식으로 진행하였다. 입자상 알루미늄 충전복극조를 이용하여 T-P 농도 10 mg/L인 인산염 함유 용액을 6 V에서 3 h 회분식으로 전해처리 한 결과 T-P의 제거율은 88% 이었다. 입자상 알루미늄 충전복극조를 이용하여 T-P 농도 10 mg/L인 인산염 함유 용액을 6 V에서 HRT를 3 h으로 하여 연속식으로 전해처리 한 결과 T-P의 농도는 2 mg/L이었다. HRT 3 h으로 고정하여 전해처리 할 경우 120 h 처리 후 파과점을 관찰할 수 있었다.