• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.6
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• pp.41-61
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• 2019

The purpose of this study was carried out to present the basic data for constructing a ecological stream, Shindangcheon stream located in Gyeongju-si, Gyeongsangbuk-do, South Korea. From August 2017 to June 2019, a total of 7 surveys were conducted. The numbers of vascular plants were summarized as 388 taxa including 75 families, 246 genera, 360 species, 4 subspecies, 19 varieties, 2 forms and 3 hybrids. The most families of taxa were Poaceae (59 taxa), Asteraceae (47 taxa), Fabaceae (27 taxa), Rosaceae and Cyperaceae (20 taxa, respectively), and Polygonaceae and Brassicaceae (15 taxa, respectively). The rare plant was Aristolochia contorta Bunge, and the Korean endemic plants were 4 taxa, such as Populus × tomentiglandulosa T.B.Lee, Salix koriyanagi Kimura ex Goerz, Lespedeza maritima Nakai, and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region found in this site were 13 taxa comprising 8 taxa of grade I, 4 taxa of grade III, and 1 taxon of grade IV. The naturalized plants were 73 taxa, such as Fallopia dumetorum (L.) Holub, Persicaria orientalis (L.) Spach., Potentilla supina L. var. ternata Pterm., and so forth. The invasive alien plants were 6 taxa, such as Rumex acetosella L., Sicyos angulatus L., Solanum carolinense L., Ambrosia artemisiifolia L., Lactuca serriola L., and Symphyotrichum pilosum (Willd.) G.L.Nesom..

• The Korean Journal of Food And Nutrition
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• v.30 no.6
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• pp.1292-1298
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• 2017

The purpose of this study was to evaluate the antioxidant and anti-adipogenic activities of Ligularia stenocephala (L. stenocephala) extract. The contents of the total polyphenol of the extract was 55.950 mg GAE/g residue. Antioxidant activities of L. stenocephala were evaluated by free radical scavenging ability and a reducing power test. 2,2'azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ${\alpha}$-${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) free radical scavenging activities of the extract were approximately 90% and 70%, respectively. Reducing power of the extract was 258.833 mg TE/g residue. The anti-adipogenic activity of L. stenocephala extract was examined in 3T3-L1 cells. During adipocyte differentiation, the 3T3-L1 cells were treated both with and without the extract. L. stenocephala extract suppressed the lipid accumulation in a concentration-dependent manner in the 3T3-L1 cells. The L. stenocephala extract inhibited the expression of peroxisome proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$) and adipocyte protein 2 (aP2) proteins, compared with control adipocytes. These results indicate that L. stenocephala could be regarded as a potential source natural antioxidant and an anti-obesity agent.

• Journal of Industrial Technology
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• v.27 no.B
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• pp.83-89
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• 2007

We studied runoff characteristics of combined sewer overflows in a city while it was raining. The event mean concentration (EMC) of biochemical oxygen demand (BOD), chemical oxygen demand ($COD_{Cr}$), suspended solids (SS), total nitrogen (TN), and total phosphorus (TP) in one of the combined sewer sites in Chuncheon was 63.5-211.6 mg/L, 114.9-523.8 mg/L, 70.3-436.4 mg/L, 6.4-33.0 mg/L, and 1.09-6.81 mg/L, respectively. In another combined sewer, the EMC of BOD, COD, SS, TN, and TP was 42.1-131.4 mg/L, 107.7-256.5 mg/L, 33.7-221.1 mg/L, 7.9-26.4 mg/L, and 1.16-3.91 mg/L, respectively. The ratio of the cumulative pollutant mass and the cumulative discharged volume determined using all parameters (BOD, $COD_{Cr}$, SS, TN, and TP) was over 1.0, which shows the first flush effect. Relationships between flow and loadings of BOD, $COD_{Cr}$, SS, TN, and TP were 0.90, 0.89, 0.88, 0.89, 0.92, respectively. Although the size of two areas was almost same, pollutant concentration and loading were different because of the amount of rainfall, rainfall intensity and basin area.

• Food Science and Preservation
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• v.20 no.1
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• pp.111-120
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• 2013

Sprassis crispa was cultivated using soybean curd whey, and its antimicrobial activities were examined against those of eight microorganisms that were foodborne pathogens or food-poisoning bacteria. The culture broth of soybean curd whey was superior in mycelium content (17.76 g/L) to that of the defined culture broth, and the ${\beta}$-glucan content was about 10.64 percent (w/w). The antimicrobial activities of the culture broth were confirmed against those of B. cereus, St. aureus, L. monocytogenes and S. typhimurium using the paper disk method. The antimicrobial activity was also maintained after the heat treatment and alcalase treatment. The filtrate with less than 3 kDa M.W. also showed the antimicrobial activity against four strains: B. cereus, St. aureus, L. monocytogenes and S. typhimurium. The minimum inhibitory concentration (MIC) was about 1.26 mg/mL in the B. cereus and 12.6 mg/mL in the St. aureus and L. monocytogenes. The S. typhimurium showed a MIC of 62.8 mg/mL. Thus, the culture of Sparassis crispa using soybean curd whey provides a thermally stable antimicrobial agent that can be used as a natural preservative in the biofood industry.

• Environmental Engineering Research
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• v.11 no.2
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• pp.77-83
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• 2006

The hot spring water of the north Jeonla province such as Wanggung, Jookrim, Seokjung, and Hwasim, has fluoride concentration of 3.9 mg/L, 12.7 mg/L, 1.9 mg/L, and 6.3 mg/L, respectively. These figures fairly exceed the Korean and WHO standard for potable water, which is 1.5 mg/L. Therefore, in this study, research on elimination of fluoride in a hot spring water of Jookrim region, which has the highest level of fluoride concentration level in the north Jeonla province, was carried out. In analysis of Jookrim hot spring water according to the water quality standard for potable water, pH was very high at 9.25 and the concentration of fluoride was 10 times higher than the standard at 18.2 mg/L. Other measurements were within the standard or not detected. After injecting 10g of activated carbon for elimination of fluoride, the fluoride concentration was measured at 13.5 mg/L, and when 70mL or more of alum 10 g/L solution was injected, the concentration was measured at 2.8 mg/L, and injecting 3g of lime was measured at 9 mg/L. Alum showed the best elimination performance among all individual injections. Injection of 25 mL of activated carbon and 100 mL of alum solution together reduced the fluoride concentration down to 1.3 mg/L, which is under the potable standard. Injection of lime 1g and 75 mL of alum 10 g/L solution together reduced fluoride concentration to 4.1 mg/L. From the modifying HRT, by using ion exchange resin column, the pH was stabilized when HRT was Imin and showed range of $6.7{\sim}7.8$. The fluoride concentration reduced gradually as the HRT increased, and satisfied the potable standard when HRT passed 6 min, and after 30 min HRT, the concentration of fluoride was 0.05 mg/L: almost eliminated.

• Journal of Korean Neurosurgical Society
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• v.44 no.5
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• pp.327-333
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• 2008

Objective: The purpose of this study is to verify the usefulness of the rabbit model for disc degeneration study. Materials: The L1-L2, L2-L3, L3-L4. or L4-L5 lumbar intervertebral disc (IVD) of 9 mature male New Zealand White rabbits were injured by inserting a 16-gauge needle to a depth of 5 mm in the left anterolateral annulus fibrosus while leaving L5-L6 IVD uninjured. Three other rabbits also received intradiscal injections of rabbit disc cells transfected with adenovirus and bone morphogenetic protein-2 (ad-BMP-2) at L4-L5 in addition to injury by 16-gauge needle at the L1-L2 level. Using digitized radiographs, measurements of IVD height were made and analyzed by using the disc height index (DHI). Magnetic resonance imaging (MRI) scans of the injured discs, injected discs, and uninjured L5-L6 discs were performed at 15 weeks post surgery and compared with preoperative MRI scans. Results: All twelve rabbits showed consistent results of disc degeneration within 15 weeks following annular puncture. DHIs of injured discs were significantly lower than that of the uninjured L5-L6 discs (p<0.05). The mean value of disc degeneration grade of injured discs was significantly higher than that of uninjured discs (p<0.05). The injection of disc cell transfected with ad-BMP-2 did not induce disc regeneration at 15 weeks after injection. Conclusion: This study showed that the injured disc had a significant change in DHI on simple lateral radiograph and disc degeneration grade on MRI scans within 15 weeks in all rabbits. Rabbit annular puncture model can be useful as a disc degeneration model in vivo.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.2
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• pp.93-102
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• 1970

1. On the basis of the samples collected on the eastern coast of Koje-Do from May to September, 1969, studies have been made on the growth and the relationships between the carapace length and the body length, and between the carapace length and the body weight of Penaeus japonicus Bate. 2. The mean carapace length of P. japonicus was 51mm in May, 57mm in June, 47mm in July and 50mm in September respectively. 3. As a result of the present studies two populations of P. japonicus exist in waters around Koje-Do, namely the spring and fall spawning populations. 4. The relationship between the carapace length ($\iota$) and the body length(L) and between the carapace length and the body weight (W) are indicated by the following equations: May $$L=2.6544{\iota}+3.1258$$ $$W=1.892{\iota}^{1.9844}$$ June $$L=2.8659{\iota}+2.1796$$ $$W=1.082{\iota}^{2.4323}$$ July $$L=2.5840{\iota}+3.3090$$ $$W=1.290{\iota}^{2.3094}$$ September $$L=2.4234{\iota}+4.5775$$ $$W=1.599{\iota}^{2.1857}$$ 5. With regard to the relationships between the carapace length and the body length and between the carapace length and the body weight there is no significant difference between the populations spawning in June and September. 6. The relationships between the carapace length ($\iota$) and the body length (L) and between the carapace length and the body weight (W) for the samples cultured at three different localities are indicated by the following equations: Koje-do $$L=3.7738{\iota}+0.0805\;(r=0.934)$$ $$W=0.4690{\iota}^{3.0713}$$ Oma-do $$L=2.993{\iota}+1.6455\;(r=0.990)$$ $$W=0.6328{\iota}^{2.6579}$$ Kumdang-do $$L=3.2749{\iota}+0.9055\;(r=0.983)$$ $$W=0.5768{\iota}^{2.8076}$$ 7. During the larval stages the relationship between the body length (L) and the rearing day (D) is indicated by the following equations: Zoeal stages (1-3) L=0.1279D+0.2686 (r=0.979) Mysis (1) - Post larva (6) L=0.1697D+0.5634 (r=0.994) Post-larvs (7) - Post larvs (21) L=0.1344D+1.9501 (r=0.978)

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.4
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• pp.465-469
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• 1993

This study was designed to investigate fatty acid composition of salt-fermented seafoods in Chonnam area. The seven samples were analyzed for fatty acid composition. The contents of lipid was highest in salt-fer-mented anchovies and followed by salt-fermented small mullets, salt-fermented oysters, salt-fermented sea-arrows and salt-fermented small shrimps. The ratio of fatty acid composition depended on the samples but the major fatty acids were $C_{l6:0}$, $C_{20:5}$, $C_{l6:1}$, $C_{22:6}$, and $C_{l8:1}$ among all samples. The composition of n-3 polyunsaturated fatty acids was highest in salt-fermented sea-arrows (39.11%) and followed by salt-fermented small shrimps, salt-fermented oysters, salt-fermented anchovies and salt-fermented small mullets. In n-3 polyunsaturated fatty acids, $C_{20:5}$ was relatively abundant in salt-fermented oysters (17.71%) and salt-fermented sea-arrows (16.38%), and $C_{22:6}$ in salt-fermented sea-arrows (22.22%) and salt-fermented small shrimps (15.13~21.50%). However, $C_{22:6}$ was very little in salt-fermented small mullets.l mullets.

• Applied Biological Chemistry
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• v.39 no.6
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• pp.437-442
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• 1996

Two strains of Lactobacillus(L.) casfi and one strain of L. Pentosus, which were isolated from pickles, were used to investigate in studing their characteristics of ${\beta}-galactosidase$. The preferable carbon sources and pH of the MRS media for enzyme production from L. casei No.10 was found to be 1.0% lactose and pH 7.5, from L. Pentosus No.63 was 1.0% galactose and pH 7.5, and from L. casei No.36 was 1.0% lactose and pH 6.5, respectively. The maximum enzyme production from each strain was found after 48 hours culture at $30^{\circ}C$ in a medium with preferable carbon source. The optimum reaction temperature with substrate for ${\beta}-galactosidase$ activity was found at $60^{\circ}C$ for all three strains . The stability of enzyme from L. casei No.36 was found to be at $45^{\circ}C$, from L. Pentosus No.63 was found at $55^{\circ}C$. This stability from L. casei No.36 was found at $40^{\circ}C$, but it was reduced to 60% at $55^{\circ}C$. These stabilities of enzymes remained about 90% at $40^{\circ}C$ for all three strains. The optimal pH for enzyme activities was found to be pH 6.5 for all three strains. Enzyme activity remained over 90% for L. casei No.10 at $pH\;5.0{\sim}6.0$, for L. casei No.36 at $pH\;5.0{\sim}8.0$, and for L. pentosus No.63 at $pH\;6.0{\sim}7.0$.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.12
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• pp.2149-2161
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• 2000

Nonliving Absidia coerulea and Thraustochitrium sp. were used as biosorbents to remove lead and cobalt that are one of representative pollutant in wastewater and radioactive liquid waste. The optimum pH range for maximum lead and cobalt removal was increased 6.5~11.4 and 8.6~12.0 for Absidia coerulea and 4.2~10.5 and 8.9~11.6 for Thraustochitrium sp. to compared to biosorbent-free control, pH of 8.4~11.2 and 10.5~11.5, respectively. With 1 g biosorbent/L at initial solution pH 5.0. Absidia coerulea and Thraustochitrium sp. took up lead from aqueous solutions to the extent of 104 and 125 mg/g biomass, respectively, whereas Absidia coerulea and Thraustochitrium sp. at initial pH 6.0 took up only 2 and 20 mg/g biomass of cobalt, respectively. For initial 500 mg Pb/L at initial pH 5.0. optimum amount of biosorbent for maximum lead uptake was 0.2 g/L for Absidia coerulea and Thraustochitrium sp., whereas optimum 3.0 g biosorbent/L was needed for initial 200 mg Co/L at initial pH 6.0. Absidia coerulea and Thraustochitrium sp. had higher adsorption capacity for lead than that of cobalt.