• 한국임상약학회지
• /
• 제2권1호
• /
• pp.11-22
• /
• 1992

Thymic extract showed antitumor effect to sarcoma mice with higher dose$(200{\mu}g/mouse/day$ i.p., 4weeks) but not with low dose$(5{\mu}g/mouse/day$ i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. $\%$ absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and $G_2/M$ phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to $L_2T_4$ were increased after administration of thymic extract$(2-200{\mu}g/mouse/day)$. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.

• 한국작물학회지
• /
• 제28권3호
• /
• pp.351-357
• /
• 1983

예상요인이 대두에 미치는 영향을 알기 위하여 1976년부터 1981년까지의 강우량, 강수일수, 온도등의 기상요인과 동 기간에 재배한 봉의 품종의 수량을 비롯한 중요 농경형질간의 관계를 조사하여 얻어진 경과를 요약하면 다음과 같다. 1. 중요형질의 년차에 따른 변이계수는 등숙율이 37.9%, 수량 및 주당협수는 30%, 100립중은 5.5%였다. 2. 대두 재배기간 동안의 강우량, 강수일수, 온도 등을 월별로 보면 7, 8월의 기상요인이 생육에 크게 영향을 미치며 그 중 온도조건이 가장 문제가 되었다. 3. 7월의 최고, 최저 및 평균온도와 수량 및 립중, 8월의 최고온도와 수량 및 립중, 8월의 최고, 최저 및 평균온도와 등숙율과는 부의 상관이 있었다. 4. 예상요인과 수량과의 관계를 회귀직선식으로 나타내면 7월의 평균온도와 수량은 y=-42.46X ＋1200.86, 8월의 최고온도와 수량은 y=-37.95X ＋1210.42였다. 5. 중부지방의 맥전작재배지역에서는 개화기에 해당하는 7월 21일부터 7월 31일까지의 고온이 특히 대두의 수량감소를 가져온다.

• Applied Biological Chemistry
• /
• 제38권4호
• /
• pp.364-369
• /
• 1995

목단피의 mouse 복수암에 대한 항암성을 다른 생약제의 항암성과 비교하여 연구하였다. 천연생약제(목단피, 주목, 울금, 인경쓱, 여정실, 맥문동) methanol 추출물을 hexane, chloroform ($CHCl_3$), ethylacetate (EtOAc), butanol (BuOH)로 fractionation하여 mouse leukemia L1210 cell과 Sarcoma 180 (S-180) cell에 강한 독성을 나타낸 fraction에 대해 mouse 복수암 억제실험을 실시하였다. 복수형 종양세포 S-180을 ICR mouse (male, $6{\sim}7$주령, $23g{\pm}3g$, 처리당 7마리)의 복부에 주사 ($1{\times}10^{6}$ cells/0.1 ml PBS)한 1일 후 부터 10% DMSO에 용해한 시료 ($30{\mu}g/g$ body weight)를 매일 0.1ml씩 10일간 주사하고 수명연장 효과와 몸무게의 변화를 조사하였다. 처리 fraction 중에서 목단피의 EtOAc fraction이 가장 강한 항암성을 보였는데, 수명연장에서는 대조구의 17.2일 (100%)에 비하여 28.7일로서 167%로 연장되었으며, 체중 증가율도 대조구보다 낮았다 (p<0.05). 목단피의 농도별 (5, 10, 30, $60{\mu}g/g$ body weight) 항암효과는 $30{\mu}g$에서 가장 높았고, $60{\mu}g$처리구 에서는 독성이 나타났다. 목단피의 EtOAc fraction으로부터 GC-MS에 의해 잠정적으로 동정된 2-methoxyphenol, 1-(4-hydroxy-3- methoxyphenyl)-ethanone, 8-methyl-2,4(1H,3H)-pteridinedione, 2,5-furandicarboxylic dimethyl ester 가 mouse 복수암 억제에 관련이 있는 주요 화합물로 추정된다.

• Archives of Pharmacal Research
• /
• 제15권3호
• /
• pp.256-262
• /
• 1992

For the evaluation of the role of substituents of ar-turmerone for its anticancer activity, ar-turmerone (1a) and its analogs like 2-methyl-6-(4'-methyphenyl)-2-octen-4-one (1b), 2-methyl-6-phenyl-2-hepten-4-one (1c), 2-methyl-6-phenyl-2-octen-4-one (1d) and 2 methyl-6-(trans-4'-methylcyclohexyl)-2-hepten-4-one (1e) were preparedd and their cytotoxic activities against $L_{1210}$ cell were determined. Omission of methyl group at para-position dose not variate the cytotoxicity of ar-turmerone. Elongation of alkyl group at 6-position decreases $ED_{50}$ value. Saturation of aromatic ring of ar-turmerone markedly decreases the cytotoxicity. Therefore the smaller size of alkyl group at 6-position and aromatic ring of ar-turmerone should be essential for exhibiting its anticancer activity.

• Archives of Pharmacal Research
• /
• 제21권6호
• /
• pp.738-743
• /
• 1998

Thirty six 5,8-dimethoxy-1,4-naphthoquinone derivatives, which bear unsaturated alkyl side chain with ester bond, were synthesized and tested cytotoxic activity on L1210 cells a nd antitumor activity against ICR mice bearing S-180 cells. It could be recognized that the cytotoxicities of naphthoquinones with R1, being methyl and propyl (IV1~24) were not enhanced by replacing the alkanoyls with alkenoyls. In contrast, the introduction of the alkenoyl moieties on the compounds with $R_1$=hexyl (IV25~36) resulted in the enhancement of their cytotoxicities. Replacement of alkanoyl group with an alkenoyl group generally increased the T/C value of the mice bearing S-180 cells.

• 대한핵의학회:학술대회논문집
• /
• 대한핵의학회 1997년도 제36차 춘계학술대회 초록집
• /
• pp.172-172
• /
• 1997

• 생약학회지
• /
• 제31권1호
• /
• pp.16-22
• /
• 2000

These studies were designed to determine the potential cytotoxic activity of MeOH extracts of 65 crude drugs against leukemia L1210 and $P388D_1$, cell line in vitro, of which 25 samples were selected. The n-BuOH extracts of 25 samples were measured using the same method and nine were selected. These samples were measured for the potential antitumor activity against $P388D_1$, for life span in vivo, and against sarcoma 180 for tumor weight. On the basis of results, Notoginseng Radix, Anemarrhenae Rhizoma, Albizziae Cortex, Portulacae Herba, Bupleuri Radix were evaluated the effective plant on the antitumor activity. In addition, the mixture of Notoginseng Radix and Albizziae Cortex was evaluated to enhance the antitumor activity in vivo.

• 약학회지
• /
• 제38권6호
• /
• pp.627-636
• /
• 1994

Several Pt(II) and Pt(IV) complexes of N,N'-bis(2-hydroxyethyl)ethylenediamine(2-HEen) and N,N'-bis(2-chloroethyl) ethylenediamine(2-CEen) as carrier ligand were prepared. Water soluble Pt complexes were also synthesized by modification of leaving groups. The cytotoxicity of these compounds against leukemia L1210 and P388 cell in vitro were examined. The Pt complexes containing 2-CEen showed more effective cytotoxicity than those containing 2-HEen. Through the nephrotoxicity tests on the primary cultured proximal tubular cells of rabbit kidney and human kidney cells in vitro, Pt complexes with 2-CEen showed higher than those with 2-HEen which were consistent with cytotoxicity but showed very low nephrotoxicity compared with cisplatin. Also the values of BUN and creatinine in serum of Pt complexes were reduced remarkably compared with cisplatin, therefore it can be concluded that new Pt complexes seems to have much lower nephrotoxicity than cisplatin.

• 약학회지
• /
• 제35권4호
• /
• pp.283-287
• /
• 1991

Seven derivatives of 2,3-alkyl substituted N-cyanoaziridine were stereoselectively prepared from alkyl substituted alkenes and cyanamide. NMR spectral property and conformation of N-cyanoaziridine were analysed. Protons(3.34 ppm) at 2,3 position of N-cyanoaziridines are significantly deshielded in case of cyclopentyl fused N-cyanoaziridine. These protons are getting more anisotropically shielded with increasing the fused carbocycle size of N-cyanoaziridine. Therefore chemical shift for these protons are upfield shifted to 2.80 ppm in case of cyclooctyl fused N-cyanoaziridine. Their ED$_{50}$ values aganist L$_{1210}$, cell in vitro were evaluated as 0.5~8.0 $\mu$g/ml. Conformation of carbocycle of fused N-cyanoaziridine is more important to their cytotoxicity than the increment of the strain energy of 3-membered ring.

• 생약학회지
• /
• 제25권4호통권99호
• /
• pp.311-318
• /
• 1994

From the root of Angelica decursiva-albiflora Yook, which has been used as a folk medicine for a sedative, analgesic and expectorant, four free coumarins, e.g., decursidin(I), decursin(II), umbelliferone(III) and nodakenetin(IV), and two coumarin glycosides, e.g., nodakenin(V) and decuroside I(VI) were isolate. The cytotoxicity of nodakenin(V) against L-1210 leukemia cells was less effective than cisplatin, but in the nephrotoxicity against rabbit kidney proximal tubular cell nodakenin(V) showed remarkably less nephrotoxicant than cisplatin.