• Title/Summary/Keyword: L/C

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Competitive Growth and Attachment of Listeria monocytogenes and Lactococcus lactis ssp. lactis ATCC 11454

  • Lee, Shin-Ho;Frank, Joseph-F.
    • Journal of Microbiology and Biotechnology
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    • v.2 no.2
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    • pp.73-77
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    • 1992
  • The effect of a nisin-producing Lactococcus lactis spp. lactis (L. lactis) on the growth and attachment of Listeria monocytogenes Scott A and Brie 1 on stainless steel and their growth in Brain Heart Infusion broth was determined. Viable cells of Listeria decreased rapidly after 9~12 hr of incubation at $21^{\circ}C$ and after 6~9 hr of incubation at $32^{\circ}C$ in the presence of L. lactis. The number of L. monocytogenes Scott A attached to stainless steel in pure culture was $2.5{\times}10^3/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}2.3{\times}10^3/\textrm{cm}^2{\;}at{\;}32^{\circ}C$ after 48 hr of incubation, but was only $10/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}1.1{\times}10/\textrm{cm}^2{\;}at{\;}32^{\circ}C$ in the presence of L. lactis. Results from L. monocytogenes strain Brie 1 were similar to those from strain Scott A. The population of L. monocytogenes Scott A which attached to stainless steel with previously adherent L. lactis was $1.8{\times}10^2/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}8.2{\times}10^2/\textrm{cm}^2{\;}at{\;}32^{\circ}C$, whereas the population attached to sterile stainless steel was $1.2{\times}10^3/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}2.1{\times}10^2/\textrm{cm}^2{\;}at{\;}32^{\circ}C$. For L. monocytogenes Brie 1, the attached population of the control was $1.6{\times}10^4/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}3.2{\times}10^2/\textrm{cm}^2{\;}at{\;}32^{\circ}C$, and on stainless steel with adherent L. lactis, it was $1.1{\times}10/\textrm{cm}^2{\;}at{\;}21^{\circ}C{\;}and{\;}6.9{\times}10/\textrm{cm}^2{\;}at{\;}32^{\circ}C$. Surface adherent L. lactis was less inhibitory to attachment of L. monocytogenes on stainless steel than a liquid culture inoculum. Listeria attached to stainless steel survived dry storage for 20 days both in the presence and absence of adherent lactococci.

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Using Tabu Search for L(2,1)-coloring Problem of Graphs with Diameter 2 (Tabu Search를 이용한 지름이 2인 그래프에 대한 L(2,1)-coloring 문제 해결)

  • Kim, SoJeong;Kim, ChanSoo;Han, KeunHee
    • Journal of Digital Convergence
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    • v.20 no.2
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    • pp.345-351
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    • 2022
  • For simple undirected graph G=(V,E), L(2,1)-coloring of G is a nonnegative real-valued function f : V → [0,1,…,k] such that whenever vertices x and y are adjacent in G then |f(x)-f(y)|≥ 2 and whenever the distance between x and y is 2, |f(x)-f(y)|≥ 1. For a given L(2,1)-coloring c of graph G, the c-span is λ(c) = max{|c(v)-c(v)||u,v∈V}. L(2,1)-coloring number λ(G) = min{λ(c)} where the minimum is taken over all L(2,1)-coloring c of graph G. In this paper, based on Harary's Theorem, we use Tabu Search to figure out the existence of Hamiltonian Path in a complementary graph and confirmed that if λ(G) is equal to n(=|V|).

Nitric Oxide/cGMP-Independent Vasorelaxation Enhanced by L-Arginine (L-Arginine의 산화질소생성과 무관한 혈관이완효과)

  • 문승호;이종은;유광재;오봉석;이동준
    • Journal of Chest Surgery
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    • v.31 no.2
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    • pp.102-107
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    • 1998
  • It has not been clear whether L-arginine plays solely a role contributing to vascular nitric oxide (NO) synthesis. To investigate the mechanisms by which L-arginine induces vasorelaxation, effects of L-arginine on the isometric tension, and tissue NOx and cyclic guanosine monophosphate(cGMP) contents were examined in the isolated rat thoracic aorta. L-Arginine induced a dose-dependent relaxation of aortic rings only with intact endothelium only. The vasorelaxation response to low concentrations of L-arginine was abolished by the pretreatment with NG-nitro-L-arginine methyl ester(L-NAME, 10-4 mol/L), whereas the relaxation caused by higher concentrations L-arginine(10-5-10-3 mol/L) was maintained and even more pronounced in the presence of L-NAME. L-Arginine did not affect the vascular tension precontracted with KCl. The vascular tissue contents of NOx/cGMP were not significantly affected by L-arginine, while they were decreased by L-NAME. L-Arginine could not completely recover the NOx/cGMP decreased by L-NAME. Methylene blue only partially antagonized the relaxation response to L-arginine. Indomethacin did not affect the L-arginine-induced vasorelaxation, whereas ouabain markedly attenuated the relaxation. It is suggested that L-arginine induces vasorelaxation not only through its contribution to NO synthesis, but also through enhancing another endothelium-dependent mechanism which is NO/cGMP-independent and cyclooxygenase- independent.

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The RMS Current Stress Reduction Technique in Link Capacitor of Two-stage AC/DC Converter (2단 AC/DC 컨버터의 링크 캐패시터 전류 스트레스 저감 기법)

  • Jang, Doo-Hee;Jung, Young-Jin;Roh, Chung-Wook;Hong, Sung-Soo;Han, Sang-Kyoo
    • The Transactions of the Korean Institute of Power Electronics
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    • v.14 no.6
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    • pp.449-456
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    • 2009
  • The RMS(Root Mean Square) current stress reduction technique for the PFC link capacitor is proposed. Although the various parameter is exist for optimizing the link capacitor, the RMS current stress is the most weighty practical parameter. The proposed C-L filter can reduce effectively the RMS current stress by filtering the output current. And with the C-L-L-C filter proposed in this paper, the more RMS current stress can be reduce because it filters not only the output current, like C-L filter, but also the input current of DC/DC stage. The proposed filter is simple to design and have no effect on the control part of the PFC because of the very low crossover frequency. To confirm the validity of proposed filter, theoretical analysis, the design guide, verification of experimental results are presented.

A Method of Interoperating Heterogeneous Simulation Middleware for L-V-C Combined Environment (L-V-C 통합 환경 실현을 위한 이기종 시뮬레이션 미들웨어 연동 방안)

  • Cho, Kunryun;No, Giseop;Jung, Sihyun;Keerativoranan, Nopphon;Kim, Chongkwon
    • Journal of KIISE
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    • v.42 no.2
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    • pp.213-219
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    • 2015
  • Simulation is used these days to verify the hypothesis or the new technology. In particular, National Defense Modeling & Simulation (M&S) is used to predict wartime situation and conduct the military training. National Defense M&S can be divided into three parts, live simulation, virtual simulation, and constructive simulation. Live simulation is based on the real environment, which allows more realistic sumulation; however, it has decreased budget efficiency, but reduced depictions of reality. In contrast, virtual and constructive simulations which are based on the virtual environment, have increased budget efficiency, but reduced depictions of reality. Thus, if the three parts of the M&S are combined to make the L-V-C combined environment, the disadvantages of each simulation can be complemented to increases the quality of the simulation. In this paper, a method of interworking heterogeneous simulation middeware for L-V-C combined environment is proposed, and the test results of interworking between Data Distribution Service (DDS) and High Level Architecture (HLA) are shown.

Synthesis and Solution Chemistry of Metal Hydrides from Cationic Rhodium(I) Catalyst Precursors [(L-L)Rh(NBD)]$ClO_4 (L-L=Fe({\eta}^5-C_5H_4PBu^t\;_2)_2,\;Fe{\eta}^5-C_5H_4PPhBu^t)_2)$

  • Tae-Jeong Kim
    • Bulletin of the Korean Chemical Society
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    • v.11 no.2
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    • pp.134-139
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    • 1990
  • The hydrogenation catalyst precursors $[(L-L)Rh(NBD)]ClO_4\;(L-L=Fe({\eta}^5-C_5H_4PBu^t\;_2)_2,\;Fe({\eta}^5-C_5H_4PPhBu^t)_2$; NBD = norbornadiene) react with $H_2(1\;atm,\;30^{\circ}C$, MeOH) to yield $[(L-L)HRh({\mu}-H)_3RhH(L-L)]ClO_4$. These hydrido species are fluxional, and variable temperature NMR studies show the existence of a number of equilibria involving both fluxional and non-fluxional species. The synthesis, solution structures, and fluxional behaviors of these hydrides are described.

A Study on the develpoment of new recipes of 5-Korean Wild Vegetables (한국산 야생식용식물의 조리과학적 연구)

  • Lim, Sook-Ja;Park, North-Jung
    • Korean journal of food and cookery science
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    • v.10 no.4
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    • pp.412-419
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    • 1994
  • The Contents of proximate compositions, vitamin C and minerals(Ca, P, Fe and Zn) of five Korean wild vegetables were analyzed. Twenty four cooking recipes with three of the vegetables were developed and their taste characteristics were evaluated by 12-trained panels. The five Korean wild vegetables are Cassia tora(C.t.), Lycium chinese Mill(L.c.), Trichosanthes quadricirra MIQ(T.q.), Polygonatum japonicum MORR et DECAIS(P.j.) and Articum lappa L.(A.l.). They have been known as the efficacious plants agaginst diabetes mellitus. In our previous work, their hypoglycemic effects have been studied through streptozotocin-induced diabetic rats and fed on the wild vegetables. Contents of moisture were 14~86% in the samples and L.c. showed relatively higher content of crude protein(16.4%). High level of crude fat was seen in C.t.(9.4%). Contents of vitamin C were also relatively high in the wild vegetables(14.2~42.0 mg%). Analysis of minerals revealed the high level of Ca in L.c. leaves, P in A.l. and Fe in C.t. and L.c. leaves. All of the samples contained Zn in 5.0~14.6 mg%. Twelvetrained taste panels indicated that the 24-preparations of L.c. leaves, fruites and P.j. were well accepted in taste, color and odor. Although L.c. fruit gruel, L.c. cream soup and P.j. saute were evalulated as less acceptable, their sensory scores were in 3.6~5.4 which were not undesirable. The newly developed cooking recipes of L.c. fruits and leaves showed better scores in color and texture, P.j. in texture and odor.

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The Patterns of Vowel Insertion in Korean Speakers' Production of English C+/l/ and C+/r/ Clusters

  • Kang, Seo-Yoon
    • Phonetics and Speech Sciences
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    • v.4 no.4
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    • pp.3-17
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    • 2012
  • This study examines Korean speakers' production of English consonant clusters, focusing on vowel insertion. An acoustic analysis along with a statistical test was carried out to see what factors are involved in this production. The following factors were considered in the present study: phonetic properties, L1 transfer, and cluster types. Specifically, liquid types were considered to see if they cause any difference depending on C+/l/ or C+/r/ clusters in the onset in terms of vowel insertion patterns. That is, it was examined which Korean speakers produce better, C+/l/ or C+/r/ clusters. Interestingly, the result of the present experiment shows that the correct answer percent was higher in the C+/r/ onset clusters than C+/l/ onset clusters unlike Eckman's (1977) Marked Differential Hypothesis. In other words, the occurrence of the vowel insertion in C+/l/ clusters is higher than C+/r/ onset clusters. This may be attributed to L1 transfer. Furthermore, in the present study, three patterns of vowel insertion in the C+/l/ clusters were identified by implementing an acoustic analysis based on vowel duration and formant: a) vowel insertion with gemination, b) phonological epenthesis, and c) phonetic intrusion. However, phonetic intrusion mainly occurred in the C+/r/ clusters. Data were collected from 54 Korean speakers to see what factors are involved in vowel insertion patterns in the production of English consonant clusters. This study provides evidence for L1 transfer, the duration effect of /l/ in a different context, and three kinds of vowel insertion patterns in conjunction with gestural coordination by age groups.

The low temperature plasma nitrocarburizng of AISI304L and AISI316L stainless steel (AISI304L과 AISI316L강의 저온프라즈마 질탄화 처리 )

  • Jeong, Gwang-Ho;Lee, In-Seop
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2007.11a
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    • pp.153-154
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    • 2007
  • AISI316L강은 AISI304L강과 기본 조성은 같지만 316L강이 약 2.5%의 Mo가 첨가되어 있다. 저온 플라즈마 질탄화 시 모재에 첨가된 Mo의 영향을 조사하기 위하여 처리온도를 변화시켜 실험하였다. 같은 처리온도의 경우 경화층의 두께는 316L강이 비교적 두껍게 형성되었다. 316L강의 경우 $450^{\circ}C$이하에서 약 25 ${\mu}m$까지 형성되었고, 306L강의 경우 $400^{\circ}C$에서 약 10 ${\mu}m$까지 형성되었다. $400^{\circ}C$ 이하에서 경화층은 두 가지시편 모두 확장된 오스테나이트 (${\gamma}_N,\;{\gamma}_c$)로 이루어져 있으나, 304L의 경우 $430^{\circ}C$부터 석출물(CrN)이 형성되기 시작하였다. 316L의 경우 $450^{\circ}C$까지 석출물이 형성되지 않았고, $480^{\circ}C$에서 석출물 (CrN)이 관찰되었다. 석출물이 형성된 시편을 제외한 모든 시편의 내식성은 모제보다 증가 하였다.

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Expression of Canavalia Iineata Leghemoglobin cDNA in Transgenic Nicotiana tabacum (형질전환된 담배에서 해녀콩 Leghemoglobin cDNA의 발현)

  • 이선영
    • Journal of Plant Biology
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    • v.38 no.2
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    • pp.203-209
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    • 1995
  • Tobacco (Nicotiana tahacum L. cv. Wisconsin 38) leaf discs were cocultivated with Agrohacterium carrying a leghemoglobin (Lb) cDNA from Canavalia lineata. Seven plants were regenerated from the transformed leaf discs on MS media supplemented with 0.5 mg/L BAP, 0.1 mg/L ${\alpha}-NAA$, 200 mg/L kanamycin and 500 mg/L carbenicillin. Southern hybridization and PCR of genomic DNA from transgenic plants showed that the Lb cDNA was stably integrated into the genome of the tobacco. Total RNA from the transgenic tobacco showed northern hybridization signal at 1,000 nt and PCR of the first strand cDNA synthesized from the total RNA amplified 0.5 kb Lb cDNA. Furthermore, western hybridization using a polyclonal antibody against soybean Lb showed a 15.8 kD LB-like band on SDS-PAGE of proteins from the transformed tobacco. These results demonstrated that the Lb cDNA of C. lineata was not only incorporated into the genome of tobacco, but also transcribed into mRNA and translated into Lb protein in the transformed tabacco.

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