• 한국미생물·생명공학회지
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• 제18권3호
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• pp.251-259
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• 1990

생전분 분해력이 강력한 glucoamylase를 생산하는 균주로서 Asp.usamii IAM 2185를 선정하였다. 밀기울배지에서의 효소생산의 최적 initial pH는 6.0-8.0, 최적 배양온도는 25-$30^{\circ}C$, 최적 배양시간은 72시간이고, 밀기울배지에 ammonuim nitrate와 albumin의 첨가는 효소의 생산을 약간 증가시켰다. 황산암모늄분획, CM-cellulose와 DEAE-cellulose column chromatography에 의하여 효소를 정제하였고, 정제효소의 specific activity는 34.3U/mg.protein, 수율은 10.3 이었다.

• 한국염색가공학회지
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• 제16권3호
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• pp.22-30
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• 2004

The polyester fabrics were treated with the chitosan with various solubility in optimized treatment condition. The treatment method was discussed to be a high functional finishing for the polyester fabric to obtain the high moisture absorption and anti-microorganism property by evaluating the effect of the chitosan purification method on the yield and anti-microorganism property of the chitosan. On the other hand, soluble polyurethane was added to the chitosan treatment solution and/or plasma pretreatment was done. The addition of soluble polyurethane give a high add-on ratio as well as a linen like effect of treated polyester fabric. The results were as follows: 1. In the treatment of polyester fabric by the chitosan solution, a soluble PU resin and low temperature plasma treatment were done to obtain high binding force between the fabrics and the chitosan. The add-on rate and the moisture absorption ratio of the fabrics treated with the chitosan-PU after treated with the plasma slightly increased more than those of the fabrics treated with the chitosan only. 2. Anti-static property of the fabrics treated with the chitosan decreased rapidly with increasing of the chitosan concentration. The washing fastness of the fabrics treated with the chitosan-PU after treated with the plasma was better than those of the fabrics treated with chitosan only. The wrinkle resistance of the treated fabrics decreased constantly with the concentration of the chitosan. The bending rigidity of the treated fabrics increased greatly. On the treatment of polyester fabric under optimum condition, the microorganism reduction rate kept above 90％ after 10times launderings. 3. As the polyester fabrics which has flat yam was used as a weft yams were treated with the chitosan-PU as give a functional finishing effects such as durability, moisture absorption, anti-static and anti- microorganism property. Treated polyester fabric showed a good functional finishing effect and a linen like property.

• 대한약침학회지
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• 제21권3호
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• pp.203-206
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• 2018

Objectives: There is an increasing number of complex diseases that are progressively more difficult to be controlled using the conventional "single compound, single target" approach as demonstrated in our current modern drug development. TCM might be the new cornerstone of treatment alternative when the current treatment option is no longer as effective or that we have exhausted it as an option. Orthogonal stimulus-response compatibility group study is one of the most frequently employed formulas to produce optimal herbal combination for treatment of multi-syndromic diseases. This approach could solve the relatively low efficacy single drug therapy usage and chronic adverse effects caused by long terms administration of drugs that has been reported in the field of pharmacology and medicine Methods: The present review was based on the Science Direct database search for those related to the TCM and the development of antihypertensive TCM herbal combination using orthogonal stimulus-response compatibility group studies approach. Results: Recent studies have demonstrated that the orthogonal stimulus-response compatibility group study approach was most frequently used to formulate TCM herbal combination based on the TCM principles upon the selection of herbs, and the resulting formulated TCM formula exhibited desired outcomes in treating one of global concerned complex multi-syndromic diseases, the hypertension. These promising therapeutic effects were claimed to have been attributed by the holistic signaling mechanism pathways employed by the crude combination of herbs. Conclusion: The present review could serve as a guide and prove the feasibility of TCM principles to be used for future pharmacological drug research development.

• International Journal of Oral Biology
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• 제45권1호
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• pp.25-31
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• 2020

Enamel knot (EK)-a signaling center-refers to a transient morphological structure comprising epithelial tissue. EK is believed to regulate tooth development in early organogenesis without its own cellular alterations, including proliferation and differentiation. EKs show a very simple but conserved structure and share functions with teeth of recently evolved vertebrates, suggesting conserved signaling in certain organs, such as functional teeth, through the course of evolution. In this study, we examined the expression patterns of key EK-specific genes including Dusp26, Fat4, Meis2, Sln, and Zpld1 during mice embryogenesis. Expression patterns of these genes may reveal putative differentiation mechanisms underlying tooth morphogenesis.

• 한국조리학회지
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• 제17권1호
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• pp.238-247
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• 2011

본 실험은 streptozotocin(STZ)으로 당뇨병이 유도된 쥐의 혈장 요소질소량, 지질수준 및 간 지질수준, 간 효소 활성에 천연 기능성 소재 화합물이 미치는 영향을 평가하는 것이 목적이었다. 총 콜레스테롤은 기능성 소재를 보충 급여한 실험군에서 유의적으로 낮은 수치(70.69 mg/dL)를 보였으며, 이로 인해 총 콜레스테롤에 대한 HDL 콜레스테롤의 비율이 증가(42.6에서 51.5%로) 하였다. 그러나, SOD, CAT, GSH-Px, GSH 및 LPO 활성에는 변화가 없었으며, 이는 기능성 소재의 보충 급여가 당뇨 쥐의 산화적 스트레스에는 큰 영향을 주지 않음을 의미한다. 보충 급여가 당뇨 쥐의 AST 수치 감소시켰으며, 이는 천연 기능성 소재 화합물 섭취가 간 기능 손상을 입은 STZ 유발 당뇨 쥐에 회복작용을 함을 알 수 있었다.

• 한국항해항만학회지
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• 제27권4호
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• pp.485-489
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• 2003

우리나라 서남권에 해당하는 서해안고속도로가 통과하는 전라북도 고창지역은 점토분이 많아 내포돼 있는 황토가 많다. 황토는 수박이나 고구마 같은 작물의 재배에는 양질의 토사이지만, 도로를 구축하기 위한 노상재료로 사용하는데는 원하는 강도값을 얻기가 힘들 토사였다. 이 연구 대상구간의 지역은 절토부의 노상재료가 원하는 재료의 품질기준을 충족시키지 못하였다. 노상층의 재료 특성을 보면, ＃200번체 통과량이 25～82％정도로 지하수위가 거의 노상면과 일치하여 자연함수비 과다로 시공시 노상토의 지지력 확보가 불가능한 것으로 판단되었다. 본 연구는 이와 같이 지지력이 부족한 현장에 적절한 절토부 노상지지력의 확보방안을 수립하여 원하는 공정을 추진함으로서 보다 안전하고 튼튼한 고속도로를 건설하는데 있었다.

• 한국재료학회지
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• 제21권11호
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• pp.604-610
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• 2011

The effect of $BaF_2$ flux in $Y_3Al_5O_{12}:Ce^{3+}$(YAG:Ce) formation was investigated. Phase transformation of $Y_3Al_5O_{12}$(YAG) was characterized by using XRD, SEM, and TEM-EDS, and it was revealed that the sequential formation of the $Y_4Al_2O_9$(YAM), $YAlO_3$(YAP) and $Y_3Al_5O_{12}$(YAG) in the temperature range of 1000-1500$^{\circ}C$. Single phase of YAG was revealed from 1300$^{\circ}C$. In order to find out the effect of $BaF_2$ flux, three modeling experiments between starting materials (1.5$Al_2O_3$-2.5$Y_2O_3$, $Y_2O_3$-$BaF_2$, and $Al_2O_3$-$BaF_2$) were done. These modeling experiments showed that the nucleation process occurs via the dissolution-precipitation mechanism, whereas the grain growth process is controlled via the liquid-phase diffusion route. YAG:Ce phosphor particles prepared using a proposed technique exhibit a spherical shape, high crystallinity, and an emission intensity. According to the experimental results conducted in this investigation, 5% of $BaF_2$ was the best concentration for physical, chemical and optical properties of $Y_3Al_5O_{12}:Ce^{3+}$(YAG:Ce) that is approximately 10-15% greater than that of commercial phosphor powder.

• International Journal of Oral Biology
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• 제45권4호
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• pp.190-196
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• 2020

Several factors, including genetic and environmental insults, impede protein folding and secretion in the endoplasmic reticulum (ER). Accumulation of unfolded or mis-folded protein in the ER manifests as ER stress. To cope with this morbid condition of the ER, recent data has suggested that the intracellular event of an unfolded protein response plays a critical role in managing the secretory load and maintaining proteostasis in the ER. Tauroursodeoxycholic acid (TUDCA) is a chemical chaperone and hydrophilic bile acid that is known to inhibit apoptosis by attenuating ER stress. Numerous studies have revealed that TUDCA affects hepatic diseases, obesity, and inflammatory illnesses. Recently, molecular regulation of ER stress in tooth development, especially during the secretory stage, has been studied. Therefore, in this study, we examined the developmental role of ER stress regulation in tooth morphogenesis using in vitro organ cultivation methods with a chemical chaperone treatment, TUDCA. Altered cellular events including proliferation, apoptosis, and dentinogenesis were examined using immunostaining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. In addition, altered localization patterns of the formation of hard tissue matrices related to molecules, including amelogenin and nestin, were examined to assess their morphological changes. Based on our findings, modulating the role of the chemical chaperone TUDCA in tooth morphogenesis, especially through the modulation of cellular proliferation and apoptosis, could be applied as a supporting data for tooth regeneration for future studies.

• 한국미생물·생명공학회지
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• 제10권2호
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• pp.123-132
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• 1982

Bacillus circulans F-2의 정제$\alpha$-amylase(F-2A)의 효소적특성과 각종 생전분의 분해율을 검토하고 다음과 같은 결과를 얻었다. 1. 분자양은 93,000, 등전점은 pH5.0부근, 최적pH는 6.0~6.5, 안정 pH범위는 5.5~12.0, 최적온도는 6$0^{\circ}C$, 4$0^{\circ}C$ 이하에서 안정하였다. 2. Mn$^{＋＋}$, Co$^{＋＋}$ 은 부활제로써 작용하였으나 Ag$^{＋}$, Hg$^{＋＋}$, Cu$^{＋＋}$은 저해제로써 작용하였다. 3, 본 효소의 활성에 SH기는 필수적이 아닌 것으로 생각된다. 4. Michaelis constant (Km)는 1.704 mg/$m\ell$ 이고 활성화에너지는 25~4$0^{\circ}C$ 에서 12.297 kcal/mole, 40~6$0^{\circ}C$ 에서 7.831kca1/mo1e이었다. 5. Soluble starch, Amylose, Amylopectin으로부터 생성되는 초기분해산물은 G$_{6}$이며 분해가 진행됨에 따라 G$_4$와 G$_2$가 생성되었다. 6. 각종 Oligosaccharide로부터 생성되는 분해산물은 다음과 같다. G$_4$$\longrightarrow$ G$_2$＋ G$_2$,G$_3$ ＋G$_1$,G$_{5}$$\longrightarrow$ G$_4$＋G$_1$,G$_{6}$$\longrightarrow$G$_4$＋ G$_2$,G$_{7}$ G$_4$,G$_{8}$$\longrightarrow$ G$_4$＋G$_4$, 7. 감자 생전분, Sago 생전분, Yam 생전분에 대하여 본효소는 Porcine pancreatic amylase와 Streptococcus bovis amylase보다 월등하게 높은 분해율을 나타내었다.

• 한국미생물·생명공학회지
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• 제38권3호
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• pp.283-288
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• 2010

본 연구에서는 최근 국내재배가 시작한 D. alata L. 경북 6호의 성분 및 색차를 D. batatas 경북 1호와 비교 분석하였으며, 메탄올 추출물 및 이의 분획물의 항산화 및 항균력을 비교 평가하였다. 먼저 경북 6호의 경우, 수분함량은 $78.02{\pm}0.16%$로 경북1호의 $82.6{\pm}0.07%$로 다소 낮았으며, 조지질 함량은 1.7배 높았다. 반면 조단백 및 조섬유 함량은 경북 1호가 경북6호보다 1.6배정도 높았다. 회분 함량은 유사하였다. 절단면의 색차는 경북 6호의 명도가 60.7, 황색도가 11.01로 경북1호의 명도 65.33, 황색도가 4.85보다 약간 어두운 황색을 띄었다. 총 polyphenol함량은 경북 6호가 평균 89.45 mg/g으로 경북 1호의 43.91 mg/g의 2배에 해당하였으며, 특히 ethylacetate 분획은 $144.1{\pm}3.20\;mg/g$의 매우 높은 함량을 나타내었다. 총 flavonoid 및 총당 함량은 비슷하였다. DPPH radical 소거능 평가 결과, 경북 6호는 ethylacetate 분획 > methanol 추출물 > butanol 분획 > hexane 분획 > 물 분획 순으로 우수하였으며, 가장 우수한 소거능을 나타낸 ethylacetate 분획의 $IC_{50}$는 $78.32\;{\mu}g/mL$이었다. 환원성 평가 결과, 물분획 > methanol 추출물 > ethylacetate 분획 > butanol 분획 > hexane 분획 순으로 나타나 수용성 물질이 강력한 환원성을 나타내었다. 한편 경북 6호의 n-hexane 및 ethylacetate 분획에서 B. subtilis, L.monocytogenes, S. epidermidis, S. aureus, P. vulgaris, 및 S. typhimurium에 대해 우수한 항균력을 나타내었다. 본 연구 결과는 국내 재배된 D. alata L.의 기초자료로 이용될 것이다.