• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.177-183
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• 2002

The study was designed to investigate the aspects of the goblet cell of the oviduct in laying Korean ring-necked pheasants by histochemical methods (PAS, alcian blue-PAS, alcian blue pH 2.5, alcian blue pH 1.0, and aldehyde fuchsin-alcian blue stain). Twelve laying Korean ring-necked pheasants were used. The results were summarized as follows. The goblet cell of the oviduct of the Korean ring-necked pheasant contained sulfated acid mucosubstances and neutral mucosubstances in the infundibulum. The compounds of sulfated acid mucosubstances, nonsulfated acid mucosubstances, and neutral mucosubstances are present in goblet cells of the magnum, vagina and openings of the tubular gland of the uterus of the oviduct in the Korean ring-necked pheasant. The goblet cell of the isthmus and uterus of the oviduct of the Korean ring-necked pheasant contained neutral mucosubstances except openings of the tubular gland of the uterus. The histochemical characteristics of secretory granules of goblet cells in oviduct of the Korean ring-necked pheasant were not related to the position of eggs in oviduct.

• Korean Journal of Environmental Biology
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• v.26 no.2
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• pp.66-75
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• 2008

The level of genetic diversity and genetic relationships among Korean ring-necked pheasant (Phasianus colchicus karpowi) habitat and subspecies have been investigated based on Inter Simple Sequence Repeat (ISSR) markers. Wild and domesticated Korean ring-necked pheasant, hybrids between domesticated Korean ring-necked and foreign subspecies, and four foreign subspecies; Chinese ring-necked (P. c. torquatus), Melanistic mutant (P. c. mut. tenebrosus), XL White (P. c. mut) and Southern green (P. c. versicolor) were used for comparison. On the basis of the results of AMOV A, 94.08% of genetic diversity in Korean ring-necked was allocated among individuals within habitat differences. Estimate of $\Phi$st, which represents the degree of genetic differentiation among habitats was 5.9%. Based on the dendrogram reconstructed by UPGMA, Yangpyung habitat of the eight habitats turned out to be distinct from others habitat. Interestingly, domesticated Korean ring-necked and hybrid mixture showed closer genetic relationship with four foreign subspecies than Korean ring-necked. As a consequence of AMOVA, 96.63% of genetic diversity in four foreign subspecies was allocated among individuals within subspecies. Estimate of $\Phi$st representing the degree of genetic differentiation among subspecies was 3.4%, which was lower than that among habitats of Korean ring-necked. The lower level of genetic difference among four foreign subspecies showed that these subspecies were genetically closer even though they were morphologically classified into four different subspecies. When seven habitats of Korean ring-necked pheasant and four foreign subspecies were divided into Korean and Foreign Pheasant Groups, respectively, more than 17% of genetic diversity was allocated between groups (about 4% among habitats/subspecies within groups). This observation implied that Korean ring-necked pheasant is genetically quite different from four foreign subspecies. On the basis of cluster analysis, three foreign subspecies (Chinese ring-necked pheasant, Melanistic mutant pheasant, and XL White pheasant) formed a distinct group with domesticated Korean ring-necked pheasant and hybrid mixture at 98% confidence interval.

• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.299-308
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• 2002

The purpose of this morphometric study was to obtain detailed quantitative information on all cell types in the testis interstitium of Korean ring-necked pheasants combined with data on changes in the steroidogenic function of the testis during the breeding and nonbreeding seasons. Animals collected during the breeding season, testis weights, sperm production, serum testosterone levels, leuteinizing hormone-stimulated testosterone secretion, and the length of the seminiferous tubules were significantly (p < 0.05) increased as compared to the nonbreeding season. Seminiferous tubules occupied 93.25% of testis volume in the breeding season. Leydig cells constituted 0.82% of the testicular volume. The mean volume of an Leydig cell was $1039{\mu}m^3$, and each testis contained about 24.53 million Leydig cells. Testes of the pheasants during the nonbreeding season displayed a 98% reduction in testis volume that was associated with a decrease in the absolute volume of seminiferous tubules (98% reduction), tubular lumen(100%), interstitium(90%), blood vessels(84%), lymphatic spaces(97%), Leydig cells(79%), mesenchymal cells(51%), and myoid cells(61%). The number of Leydig cells, mesenchymal cells, myoid cells per testis in the breeding season was higher (p < 0.05) than in the nonbreeding season. Although the average volume of a Leydig cell was 74% lower in the nonbreeding season, the average volume of a myoid and mesenchymal cell remained unchanged. These results demonstrate that there are a striking differences in the testicular structure of the Korean ring-necked pheasant in the breeding and nonbreeding seasons. Every structural parameter of the Leydig cell was pasitively correlated with both serum and LH-stimulated secretion concentrations of testosterone. Correlation of changes in hormonal status with morphometric alterations of all Leydig cell suggests that the Korean-ring necked pheasant may be used as a model to study structure-function relations in the avian testis.

• Korean Journal of Poultry Science
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• v.23 no.3
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• pp.153-160
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• 1996

Though pheasants (Korean ring-necked pheasant) have been raised for several decades, their behavior and wild nature are far from domestication. The pheasant is a seasonal breeding species and lays a limited number of eggs in a breeding season. The growth rate and feed efficiency of pheasants are very low as compaired with those of chicken for meat purpose. In addition, the breeder's access to one's herd for care is not easy. From these reasons, pheasants seem to be unsuitable for meat production in a large flock at present. However, pheasant raising is expected to increase slowly in accordance with rising demand for special poultry meat. Therefore, it is necessary to improve techniques about raising, feeding and rnanagement, so that the consumer price of pheasant meat can be lowered down reasonably.

• Korean Journal of Poultry Science
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• v.24 no.3
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• pp.107-116
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• 1997

In order to achieve optimal reproductive performance, reliable morphological and physiological basic data on the reproductive organs are desirable. Adult male Korean ring-necked pheasant in inactive(mid of January) and active state (end of April) were used in this study. In addition, five active state pheasants were received a single dose of 60Co-ray 500 rads each to damage the testes. The objective of this study was to investigate the distribution pattern of protein gene product (PGP) 9.5 and ${\alpha}$-tubulin in the pheasant testes of the active, inactive and ${\gamma}$-ray irradiated active states. The results obtained were summarized as follows 1. The seminiferous tubules collected in inactive states( mid of Jan) showed narrow lumen, and the spermatogonia and the Sertoli cell were well preserved. The PGP 9.5 immunoreactivity of these tubules showed a positive reaction in paranucleus area of the spermatogonia, and a positive reaction in a small number of the Leydig cells in the interstitium of the seminiferous tubules. 2. The seminiferous tubules were dilated in active state(end of April) as compared with the inactive state. The PGP 9.5 reactivity in these tubules showed a positive reaction in many Leydig cells in the interstitium of the seminiferous tubules, and the testes of ${\gamma}$-ray irradiated group showed partially weak reaction in the interstitium of the seminiferous tubules. 3. The ${\alpha}$-tubulin reactivity in the seminiferous tubules of the inactive testes was strongly positive in the cytoplasmic process of the Sertoli cell from the basal stem region to the apical ex-tension. From the broad part of the stem region to the luminal space, the active testes showed a strong positive reaction. The ${\gamma}$-ray irradiated groups showed diminished reaction in the basal region.

• Korean Journal of Poultry Science
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• v.22 no.1
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• pp.7-13
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• 1995

To investigate the response of egg production to the artificial light to induce early egg laying. and the changes of egg weight and egg shape during the egg laying cycle, 30 Korean ring-necked pullets and their 1,284 eggs layed under artificial light control were used in this study. The first egg was ohserved at the age of 31 wk, after 4 wk of stimulating light(16 hour light: 8 hour dark). Egg production rate during seven 2-wk periods from the begining of the first egg was 43.7% and a clear peak egg production(61.7%) was shown at the 4th 2-wk period. The effects of egg production period and pullet on the egg weight, egg length, egg width and egg shape index(width/length) were significant(P<0.01). In the egg production cycle. egg weight, egg length and egg width increased steadly with time. but the value of egg shape index increased up to the 3rd 2-wk period and then decreased. Repeatabilities from intraclass correlation for the egg weight, egg length. egg width and egg shape index were 0.61. 0.53, 0.49 and 0.48, respectively.

• Journal of Embryo Transfer
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• v.29 no.4
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• pp.351-359
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• 2014

Phasianus colchicus is not only a beautiful bird but also a great value in science and under the threat of endanger. Hence, the aim of this study was to isolate the pheasant male germ cells (mGCs) and then induce them into elongated sperm-like cells in vitro. The mGCs were purified and enriched by a two-step plating method based on the different adherence velocities of mGCs and somatic cells. The percentage of the c-kit positive cells and c-kit negative cells examined by flow cytometry analysis (FCA) was 92.87% and 2.57%, respectively. Subsequently, the mGCs were induced for 48h in DMEM/F12 medium supplemented factors such as retinol acid, testosterone and bovine FSH, followed by 5 weeks in culture. We found that some elongated sperm-like cells appeared initially in vitro under inducement of stimulated factors. The elongated sperm-like cells showed in the expression of changed morphology and post-transcriptional marker such as spermatid associated (SPERT), spermatid perinuclear RNA binding protein (STRBP), round spermatid basic protein 1 (RSBN1) and SPER1L. Moreover, in DNA content identified assay, induced cells showed that the 1C DNA population markedly increased in differentiated group but it was not change in undifferentiated group. Successful in vitro differentiation of pheasant testicular germline cells into spermatids appears to offer extremely attractive potential for the conservation of endangered birds and treatment of male infertility.

• Korean Journal of Environment and Ecology
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• v.21 no.1
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• pp.30-37
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• 2007

Pellets and prey remains were analyzed to compare diets and foraging tactics of Eurasian eagle owls (Bubo bubo) in two different habitat types: forested areas and open fields. Overall 150 prey items of three taxa were identified from 66 pellets and 82 prey remains, and the birds were the most important prey in biomass (78.04%) and in frequency (56.67%). Eurasian eagle owls frequently used rats (Rattus spp.), ring-necked pheasants (Phasianus colchicus), and stripped field mice (Apodemus agrarius), but the ring-necked pheasant was most important in biomass in both habitat types. The owls generally foraged various prey in biomass but the mean mass of vertebrate prey used by the Eurasian eagle owls was 503.3g in central Korea. According to the comparison of diets in the two different habitat types, the owls used bigger and more diverse prey in forested areas than in open fields. In forested areas, the Eurasian eagle owls frequently foraged the pheasants and Mandarin ducks (Aix galericulata), but they preferred prey of particular sizes to prey of particular taxa. In open fields, however, the owls showed opportunistic foraging tactics by selecting many small mammals such as rodents or a few large birds.

• Journal of Korean Society of Forest Science
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• v.83 no.1
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• pp.6-11
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• 1994

Three different types of tissue taken from three game birds such as Ring-necked Pheasant, Rufous Turtle Dove, Mallard and a mammal, Korean Water Deer were analyzed for five heavy metals such as iron, zinc, copper, lead and cadmium. The main purpose of this study was to make clear the background levels of the metal concentrations in the tissues of these game species. The metal levels were generally low and could be considered as the background levels. But some markedly high levels of lead were recorded in the muscles of birds shot by the shotgun. The implications of these results were discussed with respect to the health of people.

• Korean Journal of Environment and Ecology
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• v.29 no.3
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• pp.344-352
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• 2015

This study aims to identify the difference in the starting time of the dawn chorus of different species of birds and related environmental conditions. For this study, the bird songs were recorded every 2 hours a day starting from 2 hours before sunrise for a whole spring season (from April to June, 2014) in Korea. The recorded sounds were analyzed to find the starting time of each species' song by sonogram pattern using a sound analyzing program (Adobe Audition CC). To analyze the relationship between the environmental conditions and the time of the birdsong of each species, we collected the data of environmental conditions: daily precipitation, Julian date, time of sunrise and moonrise, temperature data and twilight time from the Korea Meteorological Office. As a result of this study, it was found that there are several statistical correlation between the starting time of the birdsong and environmental conditions (every environmental condition except daily precipitation and time of moonrise). Also, the difference of the starting time among each species were identified. The species were divided into 3 groups (early, mid and late) by their wake-up time. Ring-necked pheasant and yellow-throated bunting were categorized as 'Early' group and all woodpeckers were categorized as'Late' group. This study is significant as it first presented a classification of wild birds in Korea by the starting time of their dawn chorus. However, further studies are required to identify why each species to do their dawn singing at different times.