Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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An Immunohistochemical Study of Pheasant Testis in Active, Inactive and Damaged States

번식기, 비번식기 및 손상상태에 따른 한국꿩 고환의 면역조기화학적 연구

  • ;
  • Karl-heinz Wrobel (Instityte of Anatomh, Iniversity of Regensburg, Regensburg, Federal Republic of Germany.) ;
  • ;
  • ;
  • 양홍현 (전북대학교 수의과대학 해부학교실) ;
  • ;
  • 이영훈 (전북대학교 수의과대학 해부학교실) ;
  • 백영기 (전북대학교 수의과대학 해부학교실) ;
  • 김인식 (전북대학교 수의과대학 해부학교실)
  • Published : 1997.09.01
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Abstract

In order to achieve optimal reproductive performance, reliable morphological and physiological basic data on the reproductive organs are desirable. Adult male Korean ring-necked pheasant in inactive(mid of January) and active state (end of April) were used in this study. In addition, five active state pheasants were received a single dose of 60Co-ray 500 rads each to damage the testes. The objective of this study was to investigate the distribution pattern of protein gene product (PGP) 9.5 and ${\alpha}$-tubulin in the pheasant testes of the active, inactive and ${\gamma}$-ray irradiated active states. The results obtained were summarized as follows 1. The seminiferous tubules collected in inactive states( mid of Jan) showed narrow lumen, and the spermatogonia and the Sertoli cell were well preserved. The PGP 9.5 immunoreactivity of these tubules showed a positive reaction in paranucleus area of the spermatogonia, and a positive reaction in a small number of the Leydig cells in the interstitium of the seminiferous tubules. 2. The seminiferous tubules were dilated in active state(end of April) as compared with the inactive state. The PGP 9.5 reactivity in these tubules showed a positive reaction in many Leydig cells in the interstitium of the seminiferous tubules, and the testes of ${\gamma}$-ray irradiated group showed partially weak reaction in the interstitium of the seminiferous tubules. 3. The ${\alpha}$-tubulin reactivity in the seminiferous tubules of the inactive testes was strongly positive in the cytoplasmic process of the Sertoli cell from the basal stem region to the apical ex-tension. From the broad part of the stem region to the luminal space, the active testes showed a strong positive reaction. The ${\gamma}$-ray irradiated groups showed diminished reaction in the basal region.

Keywords

References

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