• The Plant Pathology Journal
• /
• 제25권3호
• /
• pp.241-246
• /
• 2009

Korean pine (Pinus koraiensis Sieb. et Zucc.), a five-needle pine, has recently been suffering pine wilt disease caused by non-native pinewood nematode, Bursaphelenchus xylophilus. Three-year-old Korean pine seedlings were inoculated with 10,000 pathogenic nematodes in a greenhouse to investigate disease development, water content and the density of nematodes in stems. Needle dehydration, xylem drying and pith browning started 20 days after inoculation (DAI). There were significant differences between seedlings inoculated with nematodes and control seedlings in the relative water content of stems and leaves at 20 and 30 DAI. At 60 DAI, all remaining seedlings inoculated with nematodes had died, but control seedlings all remained alive. The average number of nematodes recovered from stems of Korean pine dramatically increased from 10 to 20 DAI, and then decreased at the end of the experiment at 60 days. This study suggests that the relative water content of stems and leaves in current-year branches could be used as a useful physiological indicator for early diagnosis of pine wilt disease.

• Mycobiology
• /
• 제34권4호
• /
• pp.191-195
• /
• 2006

This study was conducted to investigate the different effects of ectomycorrhizal fungal (ECMF) species on the growth of seedlings of Pinus densiflora, and the effects of ECMF diversity on plant productivity. A total of five species of ECMF were isolated from root tips of pine seedlings collected from Mt. Songni and used as inocula. Pots containing pine seedlings were inoculated with either a single ECMF species or a mixture of five ECMF species. All of the seedlings formed ECM on their roots except for the control plants. The pine seedlings' growth responses varied by the different ECMF species. Also, pine seedlings inoculated with a mixture of five ECMF species showed the highest growth response. The results of the study suggest that the colonization of diverse species of ECMF will increase plant productivity, and the selection of suitable ECMF species could be an important factor for plant growth.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.89.1-89
• /
• 2003

Disease suppression by ectomycorrhizal(ECM) fungi has been demonstrated on red pine seedlings. Culturing of pathogenic fungi on petri plates containing culture filtrates of ECM fungi showed that culture filtrates of the ECM fungus Hebeloma cylindrosporum may inhibit the mycelial growth of all tested soil-borne plant pathogenic(SBPP) fungi upto 60％, In order to examine the effects of ECM fungi on SBPP fungi and on red pine seedlings, both symbiotic and pathogenic fungi were inoculated into the soil with red pine seedlings by three inoculation methods; pre-inoculation of SBPP fungi 10 days before inoculation of ECM fungi, simultaneous inoculation of both fungi, post-inoculation of SBPP fungi 60 days after inoculation of ECM fungi. Seedling mortality, seedling growth, and ectomycorrhizal formation by the combined treatments were examined and compared. Pine seedlings were dead by the pre-inoculation of pathogenic fungi, except Rhizina undulate which required 9-12 days, within 6 days after inoculation. Among pathogenic fungi tested, Fusarium oxysporum was the most pathogenic with the mortality of 44％. However, no dead seedlings were shown by simultaneous inoculation of both fungi or pre-inoculation of ECM fungi. In addition, pine seedlings treated by simultaneous or post-inoculation of SBPP fungi were relatively higher than those treated by pre-inoculation in diameter at root crown and the number of ectomycorrhizal roots. There were no significant differences among inoculation methods in root length and dry weight of treated seedlings. It means that ECM fungi somehow play a role in protecting primary roots of red pine seedlings against invasion by the SBPP fungi.

• 한국산림과학회지
• /
• 제98권5호
• /
• pp.558-562
• /
• 2009

The growth, carbon and nitrogen status of container grown black pine (Pinus thunbergii) seedlings were examined at various levels of foliar fertilization (control, 0.1%, 0.2%, 0.3%). Root collar diameter, height and dry weight of black pine seedlings increased significantly with increasing levels of foliar fertilization (P<0.05). Carbon concentration in needle of black pine seedlings was significantly higher in the foliar fertilization than in the control treatments (P<0.05), while other seedling components such as stem and roots were not significantly different (P>0.05) between the foliar fertilization and the control treatments. Nitrogen concentration and content were significantly greater in the foliar fertilization than in the control treatments (P<0.05). Shoot/root ratio of black pine seedlings (needle+stem dry weight/root dry weight) was greater in the foliar fertilization (2.40-2.89) than in the control treatments (1.87). However, nitrogen use efficiency was significantly lower (P<0.05) in the foliar fertilization (28-46) than in the control (111) treatments. The results indicate that morphological characteristics and nutritional status on container grown black pine seedlings were enhanced by various levels of foliar fertilization.

• The Plant Pathology Journal
• /
• 제29권1호
• /
• pp.116-122
• /
• 2013

To evaluate the mechanisms of pathogenicity and nonpathogenicity of Bursaphelenchus xylophilus and B. mucronatus isolated in South Korea, we used 4-year-old P. thunbergii seedlings and 20-cm long one-year-old stem cuttings of 5-year-old Pinus thunbergii, and studied distributions and multiplications of pine wood nematodes after inoculation. The distributions of B. xylophilus in the 20-cm pine stem cuttings were not significantly different from that of B. mucronatus. Conversely, the proliferation rate of B. xylophilus on mycelial mats of Botrytis cinerea was significantly different from that of B. mucronatus. The study using 4-year-old P. thunbergii seedlings also showed that B. mucronatus can migrate to distal portions of the pine seedlings the same as B. xylophilus, but the populations of B. xylophilus remaining in the pine seedlings were relatively larger than those of B. mucronatus. Therefore, we concluded that the pathogenicity of B. xylophilus could be strongly influenced by its ability to multiply.

• The Korean Journal of Ecology
• /
• 제22권2호
• /
• pp.69-77
• /
• 1999

Secondary vegetation. the holistically integrated system of nature and human being, is the complicated ecosystem that is composed of natural and man-created factors. Understanding the ecological function of secondary vegetation supplies us many important informations for sustainable landscape management and ecological restoration planning. In this research, we tried to examine the shape effect of vegetation patch on early structure of populations of pine and oaks. Moreover. we also tried to clarify the ecological functions of patch edge by exploring the patch effect on germination using patch index. In addition, we present the landscape structure of man -made vegetation of our study area, and setting experimental design of research. Vegetation landscape of study area is typical human disturbed landscape mainly composed of disturbance patches. Vegetation types of graveyard and managed pine forest were controlled by periodically repeated management. However, current seedlings of pine occurred well at both vegetation types. Presence of both saplings were more controlled in managed pine forest (PDM) and graveyard (G) than those of undergrowth (PD) and forest edge (FE) with canopy trees. The number of pine seedlings increased with patch size and patch perimeter. That of oak seedlings was, however, not significantly different. Larger graveyards provided higher light availability for germination of pine seedlings. We think, however, most seedlings of both species in the large sized graveyards without shade will die more easily than that of small sized ones before rainy summer. Relationships between patch shape and germination of two woody species cannot be exactly explained enough yet in these results. More informations on spatial interaction of the total species with differently sized patches are necessary to solve the concept of patch effect on species colonization.

• Mycobiology
• /
• 제46권4호
• /
• pp.388-395
• /
• 2018

Radionuclides were deposited at forest areas in eastern parts of Japan following the Fukushima Daiichi Nuclear Power Plant incident in March 2011. Ectomycorrhizal (EM) fungi have important effects on radiocaesium dynamics in forest ecosystems. We examined the effect of colonization by the EM fungus Astraeus hygrometricus on the uptake of cesium (Cs) and potassium (K) by Pinus densiflora seedlings. Pine seedlings exhibited enhanced growth after the EM formation due to the colonization by A. hygrometricus. Additionally, the shoot Cs concentration increased after the EM formation when Cs was not added to the medium. This suggests that A. hygrometricus might be able to solubilize Cs fixed to soil particles. Moreover, the shoot K concentration increased significantly after the EM formation when Cs was added. However, there were no significant differences in the root K concentration between EM and non-EM seedlings. These results suggest that different mechanisms control the transfer of Cs and K from the root to the shoot of pine seedlings.

• 한국균학회지
• /
• 제45권3호
• /
• pp.188-195
• /
• 2017

송이는 세계적으로 상용 가치가 높은 버섯이나, 아직까지 자연 채취에 의존하고 있다. 현재까지 송이 감염묘를 통해 송이를 한 개씩 발생시키는 것까지는 한국과 일본에서 성공하였다. 본 연구에서는 송이 감염묘법에서 송이균이 생존할 수 있는 소나무의 최소 크기를 결정하고자 하였다. 송이 감염묘에서 송이균이 생존한 것은 이식 2년째에 14본(50%)과 이식 1년째에 10본(71%)이었다. 이식 후 송이균이 생존한 송이 감염묘는 이식 당시 평균 수고가 25 cm (최소 12 cm ~ 최대 40 cm) 였다. 결론적으로 송이 감염묘의 수고가 최소 12 cm 이상이 되면, 야외 조건에서 송이균이 생존할 수 있을 것으로 판단되었다. 이 결과는 기존의 송이 감염묘에서 사용하는 기주식물의 크기를 획기적으로 줄여 송이 감염묘 방법을 개선하였고, 송이 인공재배를 위해 어느 정도 크기의 소나무를 활용하는 것이 좋을 지의 기준을 제시하였다.

• Journal of Plant Biology
• /
• 제37권3호
• /
• pp.301-308
• /
• 1994

The individual and combined effects of acidic rain, Mg deficiency (-Mg) and Al surplus (＋Al) on the growth of shoots and roots of pitch pine seedlings and the effect of humic substances (Lit) on Al toxicity were investigated. The growth of height and dry matter were not significantly less for pitch pine seedlings sprayed with simulated acid rain (SAR) of pH 3.5 than for those sprayed with SAR of pH 5.6. But treatments of Al and ＋Al-Mg in soil solution reduced the growth of seedlings in terms of height of shoots, and dry matter of shoots or roots. Effect of Mg deficiency on the growth of seedlings was apparent only when Al was treated simutaneously. The growth of seedlings, regardless of rain pH, decreased in the following order: control=-Mg>Lit＋Al>＋Al>＋Al-Mg. Treatments of Al and ＋Al-Mg in soil solution reduced the total length of secondary and teritary roots of seedlings regardless of rain pH, and decreased in the following order: the primary root

• The Plant Pathology Journal
• /
• 제23권2호
• /
• pp.70-75
• /
• 2007

We conducted an inoculation test using nine open-pollinated families of pine trees to evaluate their susceptibility and mortality in different densities of pine wood nematode. Three-year-old nine open-pollinated pine families were inoculated with Bursaphelenchus xylophilus at levels of 3,000, 5,000, and 7,000 nematodes/seedling in greenhouse. There were no distinct patterns in latent period among three densities of B. xylophilus in all families. Most families showed the first disease symptoms of needle discoloration within 12-15 days after inoculation. However, open-pollinated progenies of Pinus densiflora showed the longest latent period because none of one-year-old needles were wilted until 14 days after inoculation with 5,000 and 7,000 nematodes. One-year-old needles were wilted earlier than current needles in all tested families with all densities of B. xylophilus. Current needles were not wilted until 14 days after inoculation in all seedlings. The mortality of all seedlings rapidly increased from 35 days to 49 days after inoculation, and all died within 80 days except two seedlings. A 3,000 nematodes/100 ${\mu}L$ with sterilized distilled water are enough to screen 3-year-old pine seedlings for resistance to B. xylophilus.