• Clinical and Experimental Reproductive Medicine
• /
• 제51권2호
• /
• pp.135-141
• /
• 2024

Objective: Recently, oral antioxidants in combined forms have been used to treat men with idiopathic infertility. This study aimed to evaluate the effects of treatment with vitamin C, vitamin E, selenium, zinc, arginine, L-carnitine, and coenzyme Q10 on sperm quality parameters, DNA integrity, reproductive hormones, and pregnancy rates in men with infertility and idiopathic oligoasthenoteratozoospermia (OAT). Methods: A prospective study was conducted on 420 men with infertility and idiopathic OAT who took an oral supplement of antioxidant SP-Power tablets twice daily for 6 months. Semen quality, reproductive hormones, and the DNA fragmentation index (DFI) were evaluated at baseline and at 3 and 6 months after supplementation, using the World Health Organization 2021 guidelines. Results: No significant difference was observed in volume or the percentage of typical morphology during treatment. A significant improvement in sperm concentration was observed after supplementation (8.67±1.41, 12.17±1.91, and 19.01±0.86 at baseline, 3, and 6 months respectively, p<0.01). The total motility, progressive motility, and total motile sperm count also increased significantly (p<0.01), whereas the DFI decreased after 6 months. There was an increase in normal FSH levels and testosterone levels after 6 months of supplementation of antioxidant SP-Power but these differences were not statistically significant (p=not significant and p=0.06, respectively). Conclusion: Supplementation with SP-Power tablets improved sperm quality parameters, sperm DFI, some reproductive hormones, and pregnancy rates in men with infertility and idiopathic OAT, which could be attributed to the supplement's synergistic antioxidant action. Further studies are needed to determine the effects of supplementation on oxidative stress markers.

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권4호
• /
• pp.319-325
• /
• 2015

Among solute carrier proteins, the organic anion transporters (OATs) play an important role for the elimination or reabsorption of endogenous and exogenous negatively charged anionic compounds. Among OATs, SLC22A9 (hOAT7) transports estrone sulfate with high affinity. The net decrease of estrogen, especially in post-menopausal women induces rapid bone loss. The present study was performed to search the SNP within exon regions of SLC22A9 in Korean females with osteoporosis. Fifty healthy controls and 50 osteoporosis patients were screened for the genetic polymorphism in the coding region of SLC22A9 using GC-clamped PCR and denaturing gradient gel electrophoresis (DGGE). Six SNPs were found on the SLC22A9 gene from Korean women with/without osteoporosis. The SNPs were located as follows: two SNPs in the osteoporosis group (A645G and T1277C), three SNPs in the control group (G1449T, C1467T and C1487T) and one SNP in both the osteoporosis and control groups (G767A). The G767A, T1277C and C1487T SNPs result in an amino acid substitution, from synonymous vs nonsynonymous substitution arginine to glutamine (R256Q), phenylalanine to serine (F426S) and proline to leucine (P496L), respectively. The Km values and Vmax of the wild type, R256Q, P496L and F426S were 8.84, 8.87, 9.83 and $12.74{\mu}M$, and 1.97, 1.96, 2.06 and 1.55 pmol/oocyte/h, respectively. The present study demonstrates that the SLC22A9 variant F426S is causing inter-individual variation that is leading to the differences in transport of the steroid sulfate conjugate (estrone sulfate) and, therefore this could be used as a marker for certain disease including osteoporosis.

• 한국초지조사료학회지
• /
• 제15권1호
• /
• pp.73-79
• /
• 1995

A simple conservation technique baled silage making of selected froage materials was discussed in Suwon and in Muan county during 1991 - 1992. Eleven species of forage crops and pasture grasses(maize. sorghum, pearl millet, barnyardgrass, rye, barley, spring oat, Italian ryegrass, orchardgrass, alfalfa and grass-legume pasture mixtures) were harvested at different growth stage from young plant to maturity and baled in a self constructed square baling chamber, and wrapped in a 0.05mm thick polyethylene plastic film. Each bales measured by 90cm long, 60cm wide and 50cm height and weighted between 15~20kg in dry matter basis. physio-molphologcal characteristics of the plants, leaf weight ratio(LWR), leaf area ratio(LAR), stalk ratio (SR), stalk hardness(SH) and other growth parameters, were analysed and were used as a parameter to evaluate the suitability of materials for baling. Italian ryegrass including orchardgrass, alfalfa and pasture mixtures produced high quality baled silage. Silage quality point(F1ieg-point) of Italian ryegrass was improved from 63 point in crushed custom silage to 75 piont in baled silage. Meterial of grass-legume pasture mixtures showed 55 point in crushed silage and 67 point in baled silage. Fodder rye, barley, spring oat and barnyardgrass were also evaluated as a good materials for baled silage making. On the other hand, meize including sorghum and peral millet were evaluated as a not suitable materals for baling due to its high value of SR and SH. Quality of maize was excellent with 88 point in clushed silage and medium with 47 point in baled silage making.

• 한국토양비료학회지
• /
• 제47권6호
• /
• pp.553-557
• /
• 2014

The steady increase in livestock industry has greatly required the stable production of food and forage crops. As an alternative, rice-forage cropping system has been attempted in several southern areas. The present study was performed to understand whether an application of cattle-manure compost affects soil chemical properties and crop productivity in rice-forage cropping system, rice ${\rightarrow}$ summer oat ${\rightarrow}$ rye, in Jangheong county, south Jeolla province from 2013 to 2014. Treatments was composed of control (no compost), CM1 (compost application before rice transplanting), and CM2 (two-times compost application, before rice transplanting and after rice harvest), and inorganic fertilizers (N, P, and K) were equally dressed in all plots. Yields of rice were not significantly different between treatments, however, oat production was 1.25-fold higher in CM1 and CM2. Nutrient uptake amounts of rye were higher in CM2 than CM1 and control. Total nitrogen in soil was maintained stable level during crop cultivation. And soil organic matter contents in all treatments were increased by crop residue. Available P_2O_5$ and exchangeable K were increased by cattle manure application. Therefore, it suggested that the amount of nutrient by forage crop residue should be considered in rice-forage multiple cultivation.

• 미생물학회지
• /
• 제47권3호
• /
• pp.225-230
• /
• 2011

유일 탄소원으로 palm kernel meal (PKM)과 밀기울을 함유한 최소배지에서 농후배양하여 작물 재배 토양으로부터 xylan과 locust bean gum (LBG)에 대한 분해활성이 있는 균을 분리하였다. 분리균 YB-1106의 16S rRNA 유전자 염기서열을 조사한 결과 Microbacterium arabinogalactanolyticum와 98% 유사도를 보였다. 분리균의 xylanase는 밀기울, oat spelt xylan, 미강 및 xylose와 같은 부가탄소원에 의해 생산성이 증가된 반면에 mannanase는 LBG와 PKM에 의해 생산성이 증가되었다. 특히 Xylanase는 밀기울 2%를 첨가한 배지, mannanase는 1% LBG를 첨가한 배지에서 각각 생산성이 가장 높았으며 모두 정지기에서 생산이 되었다. 분리균의 배양 상등액은 $50^{\circ}C$와 pH 6.0에서 mannanase의 최대활성을 보였으며, $50^{\circ}C$와 pH 6.5에서 xylanase의 최적반응 활성을 나타냈다. Mannanase에 의해 분해된 LBG와 xylanase에 의해 분해된 xylan으로부터 각각 분해산물로 올리고당이 관찰되었다.

• 한국균학회지
• /
• 18권2호
• /
• pp.96-101
• /
• 1990

양송이 주요병해인 마이코곤병(M. perniciosa)의 생물적방제를 위한 항균성 미생물을 선발하기 위하여 실시한 시험 결과를 요약하면 다음과 같다. 1. 미생물의 분리는 Starch casein, Soybean meal, Oat meal 배지에서 균집락 형성과 균집락의 특성이 다른 배지 보다 빠르며, 다양한 미생물의 분리가 용이하였다. 2. 실내시험에서 사상균 91, 세균 342, 방사선균 556균주의 미생물을 분리 선발한 후 Oat meal 배지에서 대치배양하여 항균력이 높은 세균 12, 방사선균 71균주를 선발하였다. 3.실내에서 항균력이 있는 균주에 대해 실시한 1차 포장시험에서 5개 균주가 수량 및 항균력이 우수하였으며, 확인시험에서는 AJ-117, AJ-136, AK-139의3균주가 뚜렷한 항균력이 있었다.

• 한국미생물·생명공학회지
• /
• 제30권3호
• /
• pp.247-252
• /
• 2002

전통 발효식품인 장류로부터 분리된 균으로 manna의 분해능이 우수한 Bacillus sp. WL-3는 형태적 특성, 생화학적 성질과 165 rRNA의 염기서열 등이 Bacillus subtilis와 유사성이 높은 것으로 확인되었다. 분리균이 생산하는 mannanase는 $55^{\circ}C$와 pH 6.0에서 최대활성을 보였으며 중성 pH에서 활성도가 높았다. 배지내에 $\alpha$-cellulose, avicel, oat spelt xylan, guar gum 및 locust bean gum(LBG)과 같은 고분자성 탄수화물을 첨가할 경우 mannanase생산성이 증가되었으며, 특히 LBC 0.5%함유한 LB배지에서 9시간 배양하였을 때 배양상등액내의 효소 활성이 65.5 U/ml로 최대 효소 생산성을 나타내 LBC 첨가하지 않은 배지에서 보다 효소 생산성이 131배 이상이 증가하였다. 균이 성장하는 동안 생성된 LBG가수분해 산물이 mannanas리 생합성을 유도하는 것으로 판단된다. 활성 염색을 통하여 Bacillus sp. WL-3의 mannanase를 분석한 결과 배양상등액에는 분자량이 약 38.0 kDa으로 추정되는 한 종류의 mannanase만이 존재하였다

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.139-139
• /
• 2017

The complete chloroplast genome sequence of Avena sterilis L., a dominant wild oat species in the family Poaceae, is first reported in this study. The complete cp genome sequence of A. sterilis is 135,887 bp in length with 38.5% overall GC content and exhibits a typical quadripartite structure comprising one pair of inverted repeats (21, 603 bp) separated by a small single-copy region (12,575 bp) and a large single-copy region (80,106). The A. sterilis cp genome encodes 111 unique genes, 76 of which are protein-coding genes, 4 rRNA genes, 30 tRNA genes and 18 duplicated genes in the inverted repeat region. Nine genes contain one or two introns. Pair-wise alignments of cp genome were performed for genome-wide comparison. This newly determined cp genome sequence of A. sterilis will provide valuable information for the future breeding programs of valuable cereal crops in the family Poaceae.

• 한국잡초학회지
• /
• 제18권3호
• /
• pp.214-224
• /
• 1998

본 연구는 고소득작물인 인삼의 생산비 절감을 위한 새로운 재배법을 확립하기 위한 방안으로 인삼포의 잡초 발생을 억제시키는 allelopathic식물을 탐색, 선발하여 이용방안을 강구하기 위한 일련의 시험을 수행한 결과는 다음과 같다. 1. 볏짚, 귀리짚, 호밀짚 및 밀짚의 추출액을 인삼밭에 발생하는 주요 잡초인 털비름, 명아주, 조개풀, 바랭이, 피, 강아지풀 종자에 처리하여 발아율을 조사한 결과, 공시한 5종의 식물 영양체 추출액 모두 1% 농도 처리에서 발아 억제 효과가 크게 나타났으며, 특히 귀리짚 추출액은 공시된 모든 잡초종들에 대해 발아 억제 효과가 가장 컸다. 2. 볏짚, 호밀짚 및 밀짚의 추출액을 인삼 포장에 직접 처리한 결과, 3종의 식물 추출액 처리 모두가 무처리구인 대조구에 비해서 잡초생육이 억제되었으며, 공시한 3종의 식물 추출액 처리구에서 인삼의 근장, 근직경, 생체중, 건물중이 대조구에 비하여 좋은 것으로 나타났다.

• 한국농공학회논문집
• /
• 제53권2호
• /
• pp.61-65
• /
• 2011

Biomass is considered to be a major potential fuel and renewable resource for the future. In fact, there is high potential to produce the large amount of energy from biomass around the world. In this study, to obtain basic data for practical application of agricultural and forest by-products as fuel of heating system in agriculture, agricultural and forest biomass resources were surveyed, the pelletizer with capacity of $50\;kg{\cdot}h^{-1}$ was designed and manufactured and pellets were made by the pelletizer. High heating value, ash content, etc. of pellets made of agricultural and forest by-products were estimated. Straw of rice was the largest agricultural biomass in 2009 and the total amount of rice straw converted into energy of $299{\times}10^3$ TOE. And in 2009, amount of forest by-product converted into energy of $9,579{\times}10^3$ TOE. High heating values of pellets made of stem and seed of rape, stem of oat, rice straw and rice husk were 16,034, 16,026, 16,089, 15,650, $15,044\;kJ{\cdot}kg^{-1}$ respectively. High heating values of pellets made of agricultural by-products were average 83.6% compared to that of wood pellet. Average bulk density of pellets made of stem and seed of rape, stem of oat, rice straw and rice husk was $1,400\;kg{\cdot}m^{-3}$ ($1.4\;g{\cdot}cm^{-3}$). Ash contents of the pellets were 6.6, 7, 6.2, 5.5, 33% respectively. Rice husk pellet produced the largest ash content compared to other kinds of pellets.