• Journal of Life Science
• /
• v.21 no.9
• /
• pp.1329-1335
• /
• 2011

Korean native goats, which are characterized by black coat color, have existed on the Korean peninsula for a long time. Until now, there has been no comprehensive investigation concerning their genetic diversity, phylogenetic analysis or origin. In this study, we investigated the genetic diversity and verified phylogenetic status of the Korean native goat using the 453-bp fragment of the hypervariable fragment I (HVI) of mitochondrial DNA (mtDNA) D-loop region from 60 individuals among 5 populations. The Korean native goat showed less haplotype diversity when compared with goats from other countries. In addition, 6 haplotypes that had not been previously reported were verified in this study. In phylogenetic analyses with other country's goats, 10 haplotypes from Korean native goats were classified into mtDNA lineage A. Moreover, in a phylogenetic tree for goats which contained mtDNA lineage A, 8 of 10 haplotypes could be included in a subgroup with goats from Vietnam and an area of China. However, none of the remaining haplotypes belonged to a major group of Korean native goats and were located on different independent positions. These results suggest that almost Korean native goats aligned more closely to China and Vietnam breeds in mtDNA lineage A and there was no gene flow from other mtDNA lineages. Our results will contribute to conservation strategies and genetic breeding of Korean native goats.

• Parasites, Hosts and Diseases
• /
• v.53 no.6
• /
• pp.765-769
• /
• 2015

Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native goats on Jeju Island, Republic of Korea, and performed phylogenetic analysis based on the 16S rRNA gene sequences. Our results showed that Anaplasma infection was found mostly in adult female goats. The phylogenetic tree revealed that the 7 sequences identified in Korean native goats could belong to Anaplasma sp. and were distinct from A. marginale, A. centrale, and A. ovis. The results indicated that the sequences identified to belong to Anaplasma were closely related to sequences isolated from goats in China and were clustered within the same group. To our knowledge, this is the first study to detect Anaplasma sp. infection in Korean native goats.

• The Journal of Korean Society of Virology
• /
• v.27 no.2
• /
• pp.217-225
• /
• 1997

PCR amplication using the primers for gag, pol and env genes in BLV (bovine leukemia virus) proviral DNA and syncytium assay were carried out for the Korean native goats experimentally infected with bovine leukemia virus to investigate pathogenesis of BLV in the goats, and to establish a model animal for BLV infection. The oligonucleotide primers used in PCR revealed very high specificity. The minimal amount of FLK-BLV cellular chromosomal DNA to detect the integrated BLV proviral DNA was 10 ng. The peripheral blood lymphocytes from the goat infected with BLV were examined at regular intervals by PCR amplification and syncytium assay. Pol or gag genes were detected in none of three infected goats at the 1st week post-infection (p.i.). At the 4th week p.i., one of three goats showed the amplified gag gene. Thereafter detection rates for the genes were increased, indicating that the BLV proviral genes were integrated in all of the lymphocytes from three goats, at the 16th weeks p.i., when it was evident in syncytium assay that the lymphocytes from all of three goats were infested with infective BLV. Investigating the tissues from the necropsied goats at the 8th month p.i., the amplified BLV proviral genes and infective BLV were detected in all of the peripheral lymphocytes from three infected-goats. Among various tissues examined, the amplified BLV proviral genes were observed in spleen and superficial cervical, mandibular and retropharyngeal lymph nodes, and the infective BLV, in superficial cervical and mandibular lymph nodes. It was assumed that the Korean native goat was quite susceptible to BLV infection, indicating that the goat could be a good model animal for BLV.

• Korean Journal of Veterinary Research
• /
• v.45 no.4
• /
• pp.581-585
• /
• 2005

The purpose of this study is to establish the analgesic effects of electroacupuncture for Korean native goat. Electroacupuncture was applied to the 6 Korean native goats. In 3 of them, rumenotomy was performed, and in the other 3, laparotomy was done. The analgesic induction time was 15 to 30 minutes. The acupoints used were Tian-ping (Celestial Peace, GV-5), Bai-hui (Hundred Meetings, GV-20), left 13th thoracic nerve and left 3rd lumbar nerve. Electroacupuncture was performed in lateral recumbency. Needles were inserted 1-2 cm deep, and connected to the electroacupuncture apparatus. The electrical stimulation condition was 30 Hz and 2-6 volts. Initially, the voltage of analgesia mode was maximized in each channel. And, the output was slowly reduced to the critical point that goats could tolerate without obvious discomfort or pain. Surgical operation was done successfully under electroacupuncture analgesia in 6 Korean native goats. In addition, the changes of temperature, heart rate and respiratory rate were studied during acupuncture analgesia. For 3 months after surgery, no experimental animals showed clinical problem in 6 Korean native goats.

• Korean Journal of Veterinary Research
• /
• v.32 no.2
• /
• pp.267-273
• /
• 1992

The purpose of present study was to investigate the progesterone concentrations throughout gestation and peripartum period, and the return to the first estrus postpartum for improvement of reproductive efficiency in Korean native goats. The average length of gestation was 148 days(range : 144~154 days) and the average number of live births was 2 kids(range : 1~5 kids) in 12 Korean native goats. Progesterone concentrations were measured in blood samples taken from 12 goats every 5 days during gestation period. Plasma progesterone concentrations were 0.10 ng/ml at Day 0 of pregnancy and increased gradually until Day 20(6.58 ng/ml). Then they decreased slightly from Day 30 to 40(range : 4.32~4.82 ng/ml), increased again after Day 40 and remained thereafter until Day 140(range : 4.32~10.36 ng/ml). The progesterone levels declined sharply to basal levels at parturitum. Plasma progesterone concentrations during the pestpartum were 6.98 ng/ml at 10 days, 4.86 ng/ml at 6 days 3.18 ng/ml at 2 days before parturition, and 0.10 ng/ml at parturition, respectively. The basal levels were maintained until the first estrus postpartum. The mean intervals from parturition to the first estrus postpartum on the basis of progesterone determination and estrus detection were $100{\pm}64(mean{\pm}S.D.)$ days in 7 Korean native goats.

• Korean Journal of Veterinary Research
• /
• v.32 no.2
• /
• pp.259-265
• /
• 1992

A study was conducted to improve the reproductive performance of Korean native goats. The length of estrous cycle and plasma progesterone concentrations during each cycles were determined by both radioimmunoassay and estrus behaviours, and the results were used in the early pregnancy diagnosis. The estrous cycles were classified into the short(l8 days or shorter, average 16.7 days), normal(19 to 22 days, average 20.9 days) and long(23 days and longer, average 23.8 days)cycle. The average length of the 19 estrous cycles was 20.8 days. Plasma progesterone concentrations in 12 normal cycles were the lowest(0.10 ng/ml) at estrus, remained high from 6 to 16 days(range : 4.43~7.93 ng/ml) and drastically decreased thereafter to reach minimal concentrations at the next estrus. Plasma progesterone concentrations were measured for early pregnancy diagnosis at 0, 10 and 20 days after mating in the 12 Korean native goats. Plasma progesterone concentrations in the pregnant goats at 20 days after mating were significantly higher than in the non-pregnant goats(p<0.001). Of the 12 goats, 10 were confirmed pregnancy by both progesterone concentrations and kidding. The accuracy of the pregnancy diagnosis based on plasma progesterone concentrations was 100% for positive as well as for negative.

• Korean Journal of Veterinary Research
• /
• v.39 no.5
• /
• pp.908-920
• /
• 1999

We investigated the cytogenetic characteristics of Korean native goat(Capra hircus). Chromosome slides were prepared from peripheral blood cell cultures. GTG, GBG, RBG and CBG-banding techniques were employed on those slides. The high resolution karyotype of Korean native goat could be made with the incorporation of BrdU. Korean native goat has 60 chromosomes composed of 58 autosomes and XY or XX sex chromosomes. All of autosomes of Korean native goat were acrocentric chromosomes. X chromosome was submetacentric and Y chromosome was metacentric. The GTG, GBG and RBG-band patterns of Korean native goat were similar to those of other goats. CBG-band regions were distinct at the proximal portion of the long arms of all autosomes in Korean native goats. According to our investigation, there was no significant difference in chromosomal band patterns between Korean native goat and other goats. It might be necessary to use molecular genetic markers for clarifying the genetical characteristics of Korean native goat whose biological characteristics are not clearly defined.

• Korean Journal of Veterinary Research
• /
• v.32 no.2
• /
• pp.275-280
• /
• 1992

To investigate the age at puberty and the seasonal breeding in Korean native goats, progesterone concentrations were measured in blood. Blood samples were collected from 8 goats at 10 day intervals from 2 months of age until the first estrus after birth, and then every 5 days for a further estrous cycle and the seasonal breeding. The mean age and weight at puberty were $195{\pm}57$ days($mean{\pm}S.D.$, range : 107~260 days) and $11.1{\pm}0.9kg$(range : 9.8~12.0kg), respectively. The mean age at first pregnancy after birth was $241{\pm}109$ days(range : 107~273 days). The estrus was observed 47.6% from October to December, and was highest in fall(38.1%) and lowest in spring and summer(14.3%). However, the estrus was observed every season. About 67% of total conception occurred form October to January. The parturition occurred 41.7% in spring, 25.0% in summer and winter, and 8.3% in fall, respectively. These results suggest that Korean native goats do not have a breeding season, but the reproductive activity is influenced by the season.

• Korean Journal of Animal Reproduction
• /
• v.16 no.3
• /
• pp.231-237
• /
• 1992

The effects to the corticosteroid dexamethasone(DEX) and(or) estradiol-benzoate(E-B) treated to induction of parturition and birth weights of the young was conducted in 48 pregnant Korean native goats. The animals were divided into 4 goats per each treatment by the time(142, 145 and 148 day of pregnancy) and dosage(DEX 15, 20mg, DEX 8mg＋E-B 10mg and DEX 13mg＋E-B 7.5mg per goat). The results obtained were summarized as follows: 1 The time for induction of parturtion after DEX(15, 20mg) treatment on day 148 of pregnancy was significantly(P<0.01) shorter than 142 and 145 days. There was no significant difference each dosage of DEX on same day of pregnancy. 2. The addition of E-B to DEX treatment compared with DEX alone group at day 142 and 145 of pregnancy were significantly(P<0.01) shorter the time for induction of parturition. However, treatment with DEX along or with E-B on 148 day of pregnancy did not affect the time to induction(about 27 hrs) of parturition between each treatment. 3. The birth weight of kids after parturition was heaviest(P<0.01) on day 148 of pregnancy. However, development and vigor of kids were not significant different between DEX alone treatment and with E-B.

• Journal of the korean veterinary medical association
• /
• v.20 no.2
• /
• pp.97-102
• /
• 1984

It is difficult to find the reports on the prevalence of the internal parasitisms of the goat in Korea. In the present studies, the internal parasitisms of the Korean native goats in Chonnam area were examined mainly by fecal examinations. For the identif