• Journal of Microbiology and Biotechnology
• /
• 제25권1호
• /
• pp.127-136
• /
• 2015

Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.

• Parasites, Hosts and Diseases
• /
• 제41권2호
• /
• pp.129-133
• /
• 2003

A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-senstive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult CDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the Cskir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.

• 한국의류산업학회지
• /
• 제10권6호
• /
• pp.857-863
• /
• 2008

The purpose of this study is to analyze the types of Hibiscus patterns expressed on Aloha shirts. This study analyzed real data collected from man's aloha shirts displayed at the International Market Place in Waikiki of Hawaii during January 21-27, 2007. 35 pieces of Aloha shirts with Hibiscus Patterns were was analyzed. The total of each analysis means all number of patterns sampled or appeared to analyze the types of patterns. The motif of Hibiscus was sampled a total of 42 units for some shirts having several motifs. Its motif was divided into two groups, 20 units(47.62%) of natural type and 22 units(52.38%) of stylized type, according to modeling. Two types were much the same percentage. Hibiscus patterns were classified into three groups, isolated, band and arabesque type, by formation type. The isolated type was appeared most frequently. The layout of Hibiscus pattern was classified into three groups, engineered print, all over print and border print. All over print was appeared most frequently. The data of this study will be provided as a resource to promote using and developing of the pattern of Korean rose of Sharon.

• Molecules and Cells
• /
• 제19권3호
• /
• pp.334-341
• /
• 2005

Plants are sessile and rely on a wide variety of growth hormones to adjust growth and development in response to internal and external stimuli. We have identified a gene, designated NAN, encoding a basic helix-loop-helix (bHLH) transcription factor that regulates cell elongation and seed germination in plants. NAN has an HLH motif in its C-terminal region but does not have any other discernible homologies to bHLH proteins. A bipartite nuclear localization signal is located close to the HLH motif. An Arabidopsis mutant, nan-1D, in which NAN is activated by the insertion of the 35S enhancer, exhibits growth retardation with short hypocotyls and curled leaves. It is also characterized by reduced seed germination and apical hook formation, symptomatic of GA deficiency or disrupted GA signaling. The phenotypic effects of nan-1D were increased by treatment with paclobutrazol (PAC), an inhibitor of gibberellic acid (GA) biosynthesis. NAN is constitutively expressed throughout the life cycle. Our observations indicate that NAN has a housekeeping role in plant growth and development, particularly in seed germination and cell elongation, and that it may modulate GA signaling.

• BMB Reports
• /
• 제41권8호
• /
• pp.587-592
• /
• 2008

Cotesia vestalis is an endoparasitoid of Plutella xylostella larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we characterize the gene, CvBV3307, and its products. CvBV3307 is located on segment S33 of the CvBV genome, is 517 bp, and encodes a putative protein of 122 amino acids, including a serine-rich region. The expression pattern of CvBV3307 in parasitized larvae and the subcellular localization of CvBV3307 only in granulocytes indicated that it might be involved in early protection of parasitoid eggs from host cellular encapsulation and in manipulating the hormone titer and developmental rhythm of host larvae. Western blot analysis showed that the size of the immunoreactive protein (about 55 kDa) in parasitized hosts at 48 hours post parasitization (h p.p.) is much larger than the predicted molecular weight of 13.6 kDa, which suggests that CvBV3307 undergoes extensive post-translational modification in hosts.

• Journal of Oral Medicine and Pain
• /
• 제26권2호
• /
• pp.95-105
• /
• 2001

제 4형 콜라젠을 분해하는 MMP-9은 조직 재생에 중요한 역할을 하기 때문에 주목받아 왔는데, 이전의 문헌들에서 PMA에 의해 이 유전자 발현이 강하게 상승발현된다고 알려져 있다. 비록 MMP-9 발현을 조절하는 PMA의 기전이 잘 밝혀지지는 않았지만, 다른 유전자발현에서의 이 phorbol ester의 효과는 c-raf-1-ERK 신호전달통로의 활성에 관해 연구되어 오고 있다. 하지만 이번 연구에서 저자는 MMP-9 발현에서 PMA의 상승효과에는 대개는 스트레스성 자극과 관련된 JNK1 의존성 신호전달과정이 또한 필요하다는 다른 가능성을 조사하였다. 그 결과 JNK 억제재중 하나인 SP 600125가 UM-SCC-1세포주에서 PMA에 의해 유도된 MMP-9 상승발현을 용량의존적으로 억제하는 것으로 나타났고 72시간동안 전처치를 한 경우에 그의 억제효과가 최대이었다. phorbol ester로 처리한 세포에 GAL4 luciferase reporter와 vector를 주입해서 조사한 결과 PMA가 c-Jun transacting 활성을 상승시켰다. 그뿐 아니라 PMA에 의한 MMP-9 촉진자 활성에는 AP-1 motif가 필요하며 이 motif에 c-Jun이 결합하는 것을 EMSA를 통해 확인하였다. 결론적으로 UM-SCC-1 세포주에서 PMA에 의한 MMP9의 증가된 발현은 이미 밝혀진 ERK 경로뿐 아니라 JNK 경로를 통한 결과임이 밝혀져 이 경로를 차단하는 방법이 또하나의 암치료 방향을 제시해주고 있다.

• 가정과삶의질연구
• /
• 제20권6호
• /
• pp.129-140
• /
• 2002

The purpose of this study was to collect baseline data for women＇s needs for social education, in order to eventually contribute to improving the quality of women's social education. In the needs survey, information on the motive to participate in social education programs, obstacles to participation, and program preferences was collected. The data were then analyzed by women＇s socio-demographic characteristics. Survey participants were married women between the ages of twenty to fifty, who were sampled from Seoul, six metropolitan areas, and nine provinces. For the final analysis, 1,026 survey forms were used. The motive for participating in women＇s social education programs was examined by each category. Overall, the participants showed the highest level in educational achievement motif. The motivations for lifestyle change, self-realization, and social accomplishment were also high and at a similar level. As for obstacles to participation, social obstacle received the highest rate, followed by family obstacle and personal reasons. As for the type of social education programs, home management programs were the most preferred, followed by psychological education, family education, leisure activity programs, physical education, and social education programs.

• Journal of Plant Biotechnology
• /
• 제49권1호
• /
• pp.15-29
• /
• 2022

Cyclophilins (CYPs) are highly conserved ubiquitous proteins belong to the peptidyl prolyl cis/trans isomerase (PPIase) superfamily. These proteins are present in a wide range of organisms; they contain a highly conserved peptidyl-prolyl cis/trans isomerase domain. A comprehensive database survey identified a total of 35 genes localized in all cellular compartments of Solanum lycopersicum L., but largely in the cytosol. Sequence alignment and conserved motif analyses of the SlCYP proteins revealed a highly conserved CLD motif. Evolutionary analysis predicted the clustering of a large number of gene pairs with high sequence similarity. Expression analysis using the RNA-Seq data showed that the majority of the SlCYP genes were highly expressed in mature leaves and blooming flowers, compared with their expression in other organs. This study provides a basis for the functional characterization of individual CYP genes in the future to elucidate their role(s) in protein refolding and long-distance signaling in tomatoes and in plant biology, in general.

• Molecules and Cells
• /
• 제45권5호
• /
• pp.343-352
• /
• 2022

The advent of the assay for transposase-accessible chromatin using sequencing (ATAC-seq) has shown great potential as a leading method for analyzing the genome-wide profiling of chromatin accessibility. A comprehensive reference to the ATAC-seq dataset for disease progression is important for understanding the regulatory specificity caused by genetic or epigenetic changes. In this study, we present a genome-wide chromatin accessibility profile of 44 liver samples spanning the full histological spectrum of nonalcoholic fatty liver disease (NAFLD). We analyzed the ATAC-seq signal enrichment, fragment size distribution, and correlation coefficients according to the histological severity of NAFLD (healthy control vs steatosis vs fibrotic nonalcoholic steatohepatitis), demonstrating the high quality of the dataset. Consequently, 112,303 merged regions (genomic regions containing one or multiple overlapping peak regions) were identified. Additionally, we found differentially accessible regions (DARs) and performed transcription factor binding motif enrichment analysis and de novo motif analysis to determine new biomarker candidates. These data revealed the gene-regulatory interactions and noncoding factors that can affect NAFLD progression. In summary, our study provides a valuable resource for the human epigenome by applying an advanced approach to facilitate diagnosis and treatment by understanding the non-coding genome of NAFLD.

• 패션비즈니스
• /
• 제28권3호
• /
• pp.48-68
• /
• 2024

In recent years, with the international spread of traditional Chinese culture, traditional auspicious patterns have been used idely in modern textile design. In particular, a bat pattern has the same pronunciation as 'fu' in Chinese. It symbolizes good luck and longevity. It has auspicious meanings such as happiness, prosperity, wealth, longevity, and good luck. This study aimed to explore how cultural elements could be utilized in modern textile design by incorporating the traditional Chinese bat pattern into scarf design. Through literature research and case analysis, we aimed to understand the historical background and design characteristics of the bat pattern and develop it into a textile design suitable for modern scarf design to promote modern reinterpretation of traditional culture and cultural integration. From a design perspective, the shape of the bat pattern was analyzed to derive five main shapes and characteristics (symmetry, verticality, repetition, totality, and relativity). Based on this, 10 patterns were created by combining them with popular styles of modern scarf patterns. In addition, five product designs were completed considering 23/24 Pantone trend colors, 24spring/summer fashion trend colors, and silk scarf pattern characteristics.