• The Korean Journal of Ecology
• /
• v.19 no.5
• /
• pp.417-430
• /
• 1996

This study was conducted to investigate the developmental process of plant community during the secondary succession and the dynamics of soil properties in the burned and unburned areas of Mt. Ch’oly-san. Owing to the forest fire occurred on April, 1989, the red pine(Pinus densiflora) forest and its floor vegetation were burned down. The floristic composition of burned and unburned areas were composed of 53 and 49 species of vascular plants, respectively. The dominant species based on SDR4 of the burned sites were lespedeza cyrtobotrya (89.62), Miscanthus sinensis var. purpurascens (62.50), and Carex humilis (58.73), Quercus serrata (43.33). In contrast, Pinus densiflora (83.56), Lespedeza cyrtobotrya (55.57), Miscanthus sinensis var. purpurascens (51.88) and Carex humilis (50.41) were dominant in the unburned area. The biological spectra showed the $H-D_1-R_5-e$ type in both the burned and unburned areas. The indices of similarity ($CC_S$) between the two areas were 0.74. Degree of succession (DS) was 604 in the burned area and 802 in the unburned area. From these facts, it is assumed that the succession is rapidly progressing because of the recovery of vegetation. The species diversity ($\={H}$) and evenness index(C) in the burned and unburned areas were 0.15 and 0.18, respectively. Red pine tree did not resprout after scorch by the forest fire, but Lespedeza, Quercus, Rhododendron, Albizzia, and Zanthoxylum resprouted from the roots and trunks after the forest fire. It seems that these species are the fire-resistant species. Soil properties such as soil pH, content of organic matter, available phosphous, total nitrogen, tatal carbon, exchangeable potssium, sodium, calcium, and magnesium increased due to forest fire. These results suggest the intensity of forest fire in the study area was relatively weak. Monthly changes of soil properties were of little significance except for some cases.

• Applied Biological Chemistry
• /
• v.50 no.4
• /
• pp.344-347
• /
• 2007

The aerial parts of Lespedeza cuneata were collected, air-dried and extracted with 95% aqueous EtOH. Then it was successively partitioned with n-hexane, $CH_2Cl_2$, EtOAc and $H_2O$. Repeated Sephadex LH-20 column chromatography on the EtOAc- and $H_2O-soluble$ fractions gave four compounds. Their structures were elucidated as quercetin (1), kaempferol (2), desmodin (3) and homoadonivernith (4) on the basis of spectroscopic evidences such as $^{1}H-NMR$, $^{13}C-NMR$, 2D-NMR and MS spectrum. Desmodin (3) and homoadonivernith (4) have not been reported from this plant so far.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.1 no.1
• /
• pp.141-150
• /
• 1998

This study was conducted to find out the possibility of seeding of Zoysia japonica and woody plants for the revegetation of rock-exposed-cutslopes by Natural Topsoil Restoration Methods (NTRM). The 5cm thick layer of artificial soil was attached at rock-exposed cutslopes for the growth of Zoysia japonica and native woody plants by NTRM. The main results were summarized as follows. 1. Artificial soil attached at rock-exposed-slopes by NTRM was not eroded until recovered by Zoysia japonica and native woody plants. It shows extremely low soil erodibility. 2. One year later after seeding, woody plants show 100% ground coverage. But, in early stage, they grew more quickly at northwest slopes than at southeast slopes. 3. In seeding of Zoysia japonica, it grew more quickly at southeast slopes than at northwest slopes. Also, it grew better at lower part of the slopes than at middle part of the slopes. 4. After one year from seeding, the height of Lespedeza cyrtobotrya and Indigo/era pseudo-tinctoria were longer than any other plants. The next were Albizzia julibrissin, Lespedeza cuneata and wild flowers. The tree height of Rhus chinensis shows 28cm length, so it has enough possibility to be used for roadside revegetation works. 5. Lespedeza cyrtobotrya and Indigo/era pseudo-tinctoria show different importance values according to slope aspect. But, they show tendency to outweigh the other plants at all slope-aspects. 6. Most of the plants seeded by NTRM were germinated well and they covered rock-exposed-slopes so quickly and effectively. Thus it leads to conclusion that the revegetation methods used in this experiment was a very effective methods to recover rock-exposed-slopes by seeding of zoysia japonica and native woody plants.

• Food Science and Preservation
• /
• v.21 no.6
• /
• pp.844-850
• /
• 2014

This study was conducted to examine the physiological activities of Lespedeza cundata extracts. The extraction yield of 50% ethanol extract (17.60%) was higher than that of hot water extract (12.60%). The total phenolic and total flavonoid contents of the 50% ethanol extract were 242.26 mg/g and 160.73 mg/g, respectively. The DPPH radical scavenging activities of the hot water and 50% ethanol extracts were 92.07% and 96.38%, respectively. The superoxide radical scavenging activities of hot water and 50% ethanol extracts on $250{\sim}1,000{\mu}g/mL$ were 54.89~85.68% and 44.50~94.46%, respectively. The tyrosinase inhibition activity of the 50% ethanol extract at $1,000{\mu}g/mL$ (63.31%) was the highest. The nitrite scavenging activity of the 50% ethanol extract was higher than that of the hot water extract. The nitric oxide production of 50% ethanol extract ($7.15{\sim}20.61{\mu}M$) improved with an increase in the treatment concentration. The hot water and 50% ethanol extracts at $1,000{\mu}g/mL$ inhibited the proliferation of the cancer cell lines A549, HeLa, Hep3B, and Sarcoma180. There results suggest that the 50% ethanol Lespedeza cuneata extracts may be useful as a functional food material in the food industry.

• Korean journal of food and cookery science
• /
• v.28 no.3
• /
• pp.275-283
• /
• 2012

This study was conducted to monitor the quality characteristics of Lespedeza cuneata ethanolic extracts, by a response surface methodology. The independent variables were the extraction temperature ($35{\sim}95^{\circ}C}$), extraction time (2~10 hr), and ethanol concentration (10~90%). The coefficients of the determinations (R2) were 0.8562 (p<0.1), 0.9787 (p<0.01), and 0.8344 (p<0.1) in total polyphenol, total flavonoid, and electron donating ability, respectively. The electron donating ability and nitrite scavenging effect were improved with an increase of ethanol concentration, rather than the extraction temperature. ORAC (Oxygen Radical Absorbance Capacity) with extraction conditions was 1,636.47~2,696.99 ${\mu}moles$ TE/g, and was increased with the increase of extraction temperature and 40~60% of ethanol concentration. Estimated conditions for the maximized extraction including the yield, total polyphenol, electron donating ability, nitrite scavenging effect, and ORAC, were $60{\sim}68^{\circ}C$ in extraction temperature, 6~7 hr in extraction time, and 38~60% in ethanol concentration.

• Korean Journal of Environment and Ecology
• /
• v.19 no.2
• /
• pp.75-82
• /
• 2005

To investigate the vegetation structure of the region from Dongchanggyo to Deogjusa in Woraksan National Park, 23 plots$(400m^2)$ set up with random sampling method were surveyed. Three groups Quercus mongolica-pinus densiflora community, Pinus densiflora-Quercus molica community, Mixed Broad-leaved community were classified by cluster analysis. Quercus mongolica, pinus densiflora, Quercus serrata and Fraxinus rhynchophyllai were found as a major woody plant species in Woraksan National Park region. In this area, Quercus mongolica and Pinus densiflora were dominated partially. In the future, the importance percentage of Pinus densiflora might be decreased, but those of Quercus mongolica, Quercus serrata and Fraxinus rhynchophyllai might be increased. High positive correlations were proved between Acer pic tum and Corylus heterophylla, Betula schimidtii; Acer pseudosieboldianum, Cornus controversa ; Stephanandra incisa and Symplocos sawafutagi, Lespedeza maximowixzii ; Symplocos sawafutagi and Fraxinus rhynchophyllai, Lespedeza maximowixzii; Fraxinus rhynchophyllai and Acer pseudosieboldianum, Lespedeza maximowixzii; Quercus variabilis and Fraxinus sieboldiana; Corylus heterophylla, and Rhododendron schlippenbachii; Acer pseudo sieboldianum and Lespedeza maximowixzii, and relatively high negative corrlations was proved between Quercus serrata and Betula schimidtii; Quercus mongolica and Quercus serrata; Corylus heterophylla and Lindera erythrocarpa. Species diversity(H') of investigated groups were ranged from $1.2393\~1.3674$ and it was relatively high compared to those of the ridge area of other national parks.

• Korean Journal of Environment and Ecology
• /
• v.20 no.2
• /
• pp.114-121
• /
• 2006

To investigate the vegetation structure of mountain ridge from Miwangjae to Cheonwhangbong, 11 plots($2000m^2$) set up with random sampling method were surveyed. Three groups of Quercus mongolica community, Quercus mongolica-Styrax japonica community, Q. mongolica-Pinus densiflora community were classified by cluster analysis. Quercus mongolica was a major woody plant species in the ridge area from Miwangjae to Cheonwhangbong, and Styrax japonica and Pinus densiflora was partly occupied. High positive correlations was proved between Prunus sargentii and Euonymus alatus for. ciliato-dentatus; Quercus serrata and Euonymus alatus; Sorbus alnifolia and Euonymus alatus, Magnolia sieboldii; Acer pseudo-sieboldianum and Euonymus alatus for. ciliato-dentatus; Rhododendron schlippenbachii and Euonymus alatus for. ciliato-dentatandus; Euonymus alatus and Lindera obtusiloba, Castanea crenata, Lindera erythrocarpa; Lespedeza bicolor and Rhododendron schlippenbachii; Castanea crenata and Magnolia sieboldii; Magnolia sieboldii and Lindera erythrocarpa, and relatively high negative correlations was proved between Rhododendron schlippenbachii and Euonymus alatus; Lespedeza bicolor and Castanea crenata; Stephanandra incisa and Magnolia sieboldii; Rhododendron yedoense var. poukhanense and Lindera erythrocarpa; Euonymus alatus for. ciliato-dentatus and Magnolia sieboldii; Fraxinus sieboldiana and Sapium japonicum; Prunus sargentii and Lespedeza maximowiczii; Lespedeza maximowiczii and Lindera erythrocarpa. Species diversity(H') of investigated groups was ranged $2.3225{\sim}2.7721$, and it was relatively low value compared to that of mountain ridge area of other national parks.

• Natural Product Sciences
• /
• v.22 no.3
• /
• pp.175-179
• /
• 2016

The aim of this study was to evaluate the anti-diabetic effects of the water extract of Lespedeza cuneata (LCW) using rat insulinoma (RIN) m5F cells and streptozotocin (STZ)-induced diabetic rats. The effect of LCW on the protection of pancreatic beta cells was assessed using MTT assay, and nitric oxide production was assessed using Griess reagent. STZ-induced diabetic rats were treated with 100 and 400 mg/kg body weight of LCW for 5 weeks. In results, LCW significantly protected cytokine-induced toxicity and NO production, and increased insulin secretion in RINm5F cells. LCW significantly decreased serum blood glucose, thiobarbituric acid reactive substances (TBARS), blood urea nitrogen (BUN) and advanced glycation end products (AGEs) levels, and renal fibronectin expression in STZ-induced diabetic rats. Also, LCW effectively improved BW loss in STZ-induced diabetic rats. Thus, our results suggest that LCW has a beneficial effect on cytokine-induced pancreatic beta cell damage and biomarkers of diabetic complication in hyperglycemic rats.

• Biomolecules & Therapeutics
• /
• v.11 no.4
• /
• pp.245-248
• /
• 2003

The purpose of this study was to evaluate tyrosinase inhibitory activity of plant extracts, especially Korean indigenous plants, for the cosmetic use of skin whitening. When 50 plant extracts were tested, the methanol extracts of Agrimonia pilosa, Aster scaber; Dianthus sinensis, Fatsia japonica, Hemistepta lyrata, Lespedeza cuneata, Osmunda japonicum, Pyrvla japvnica, Rodgersia phodophylla and Veratrum grandiforum possessed the considerable tyrosinase inhibitory activity at 3-300 $\mu\textrm{g}$/mL. Especially, L. cuneata, aerial part of O. japonicum and V. gandiforum exhibited the strong inhibition (>50％ inhibition at 300 $\mu\textrm{g}$/mL). In particular, the methanol extract of V. grandiforum and its ethylacetate fraction showed potent inhibition ($IC_{50}$/=30 and 13$\mu\textrm{g}$/mL, respectively), while the reference compound, kojic acid, showed $IC_{50}$/ value of 26$\mu\textrm{g}$/mL. These plant extracts may be used as tyrosinase inhibitors in cosmetics.

• Korean Journal of Environment and Ecology
• /
• v.19 no.2
• /
• pp.106-111
• /
• 2005

To investigate the vegetation structure of trail edges in Woraksan National Park, thirty nine plots on the five trails were surveyed. Dominant species in the tree layer on trails were Quercus mongolica, Pinus densiflora and Q. variabilis, but in the shrub layer, Lespedeza maximowiczii was a dominant species on the all trails except the Dongchang section even though five trails have different situations and use of strength. The similarity index between five trails was $41.6\5%\~66.50\%$. The edge species among trails in Woraksan National Park have a similar pattern.