Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Optimization of Ethanol Extraction Conditions for Functional Components from Lespedeza cuneata Using Response Surface Methodology

반응표면분석법을 이용한 야관문(Lespedeza cuneata) 기능성분의 에탄올 추출조건 최적화

  • Kim, Dae-Ik (Biohealth convergence Center, Daegu Technopark) ;
  • Hong, Joo-Heon (Department of Food Science and Technology, Catholic University of Daegu)
  • 김대익 ((재)대구테크노파크 바이오헬스융합센터) ;
  • 홍주헌 (대구가톨릭대학교 식품가공학전공)
  • Published : 2012.06.30
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Abstract

This study was conducted to monitor the quality characteristics of Lespedeza cuneata ethanolic extracts, by a response surface methodology. The independent variables were the extraction temperature ($35{\sim}95^{\circ}C}$), extraction time (2~10 hr), and ethanol concentration (10~90%). The coefficients of the determinations (R2) were 0.8562 (p<0.1), 0.9787 (p<0.01), and 0.8344 (p<0.1) in total polyphenol, total flavonoid, and electron donating ability, respectively. The electron donating ability and nitrite scavenging effect were improved with an increase of ethanol concentration, rather than the extraction temperature. ORAC (Oxygen Radical Absorbance Capacity) with extraction conditions was 1,636.47~2,696.99 ${\mu}moles$ TE/g, and was increased with the increase of extraction temperature and 40~60% of ethanol concentration. Estimated conditions for the maximized extraction including the yield, total polyphenol, electron donating ability, nitrite scavenging effect, and ORAC, were $60{\sim}68^{\circ}C$ in extraction temperature, 6~7 hr in extraction time, and 38~60% in ethanol concentration.

본 실험은 반응표면분석법을 이용하여 야관문 에탄올 추출물의 기능성분에 대한 추출조건을 최적화하였다. 추출조건에 따른 수율의 최대값은 17.6%이었고, 중심합성계획에 의한 야관문의 추출조건별 총 폴리페놀 및 총플라보노이드 함량에 대한 반응표면 회귀식의 $R^2$는 각각 0.8562와 0.9787로 10% 및 1%이내의 유의수준에서 유의성이 인정되었다. 야관문 에탄올 추출물의 폴리페놀 및 플라보노이드 함량 변화는 추출온도와 추출시간보다는 에탄올 농도에 크게 영향을 받고 있는 것으로 나타났다. 전자공여능에 대한 회귀식의 $R^2$는 0.8344로 10% 이내의 유의수준에서 유의성이 인정되었다. pH 조건에 따른 야관문의 아질산염 소거능에서 최대점을 보인 pH 3.0에서는 추출온도가 증가할수록 에탄올 농도 범위가 40~60% 범위에서 우수함을 확인하였다. ORAC은 1,636.47~2,696.99 ${\mu}moles$ TE/g의 범위였으며, 결과에 대한 반응표면 회귀식의 $R^2$는 0.8383로 10%이내의 유의수준에서 인정되었고 추출온도보다는 추출시간과 에탄올 농도에 영향을 받는 것으로 나타났다. 야관문의 추출조건을 최적화하기 위하여 추출물의 특성인 조건별 추출물의 수율, 총 폴리페놀함량, 전자공여능, 아질산염 소거능 및 ORAC에 대해 contour map을 superimposing하여 얻은 최적조건의 범위는 추출온도 $78{\sim}85^{\circ}C$, 추출시간 6~7시간, 에탄올 농도 38~60%이었다.

Keywords

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