• Journal of Plant Biotechnology
• /
• 제38권2호
• /
• pp.151-161
• /
• 2011

Plant proteomics is the large-scale studies of proteins, particularly on their structures and functions, governed by the physiological metabolism of plant cells. With the development of techniques and strategies in proteomics, proteomics approach is moving forward in systems biology handling sophisticated components of major signaling and biochemical pathways in plants responding to their environment. In Korea, pioneers in plant proteomics are trying to catch up with global trends in plant proteomics; these researchers are not only improving existing techniques in protein extraction but also developing new techniques in proteomics context. In the hot field of abiotic and biotic stress proteomics, 29 and 9 out of 74 papers have been published during the review period from 2005 to 2010, respectively. This present review article provides an overview on the output of Korean plant proteomers while paying special attention to both abiotic and biotic stress proteomics.

• 한국환경복원기술학회지
• /
• 제23권2호
• /
• pp.69-89
• /
• 2020

Scientific trust and quantification of traditional species investigation and results that have been used in ecology for decades has always been a problem and concern for ecologists. Global ecologists have proposed DNA-based species investigation studies to find answers to problems. In this study, we reviewed the global trend of research on environmental DNA(eDNA), which is a method for monitoring species by detecting DNA of organisms naturally mixed in environmental samples such as water, soil, and feces. The first eDNA research confirmed the possibility of species investigation at the molecular level, and commercialization of NGS(Next Generation Sequencing) and DNA metabarcoding elicits efficient and quantitative species investigation results, and eDNA research is increasing in the filed of ecology. In this study, mammals and birds were detected using MiMammal universal primers from 23 samples(3 natural reserves; 20 water bowls) out of 4 patches to verify eDNA for urban ecosystems in Suwon, and eDNA was verified by performing camera trapping and field survey. Most terrestrial species were detected through eDNA, and particularly, mice(Mus musculus), and Vinous-throated Parrotbill (Sinosuthora webbiana) were identified only with eDNA, It has been confirmed to be highly effective by investigating techniques for small and internal species. However, due to the lack of resolution of the primer, weasels(Mustela sibirica) and squirrels(Melanochromis auratus) were not detected, and it was confirmed that the traditional investigation method was effective only for a few species, such as Mogera robusta(Mogera robusta). Therefore, it is judged that the effects of species investigation can be maximized only when eDNA is combined with traditional field survey and Camera trapping to complement each other.

• 한국환경과학회지
• /
• 제26권3호
• /
• pp.381-392
• /
• 2017

The present study aimed to investigate the effects of low temperature on the growth, yield, quality, and biologically active compounds of strawberry and obtain basic information for developing a technology for stable growth of strawberry in greenhouses. Growth of strawberry, including leaf number, area, and length, plant height, and dry weight was better at the optimum growth temperature of $20^{\circ}C$ than at a lower temperature of $15^{\circ}C$. At the low temperature of $15^{\circ}C$, the cultivar 'Maehyang' was more tolerant and displayed better growth rate than 'Seolhyang'. At $15^{\circ}C$, the fruit production per week and fruit weight was lower than that at $20^{\circ}C$. In contrast, fruit length and diameter were not significantly different between the two growth temperatures. Growth temperature also did not affect the fruit color index, Hunter L, a, b value, or fruit firmness. However, the sugar content of strawberries grown at $15^{\circ}C$ was higher by 0.8 and 1.5 Brix for 'Seolhyang' and 'Maehyang', respectively, than of those grown at $20^{\circ}C$. There was no difference in the content of fisetin, a biologically active compound, for 'Seolhyang' at both growth temperatures, however, the fisetin content of 'Maehyang' was higher at $20^{\circ}C$ than at $15^{\circ}C$. Cinchonine and ellagic acid content of 'Seolhyang' was higher at $20^{\circ}C$ than at $15^{\circ}C$, whereas that of 'Maehyang' was higher at $15^{\circ}C$ than at $20^{\circ}C$. Quercetin content showed no significant differences with respect to growth temperature, however, it tended to increase at $20^{\circ}C$. The cinnamic acid content of 'Seolhyang' was higher at $15^{\circ}C$ than at $20^{\circ}C$, whereas that of 'Maehyang' increased at $20^{\circ}C$. Collectively, the biologically active compounds of strawberry were affected by growth temperature. Moreover, the content of these compounds tended to increase at $20^{\circ}C$, the optimum growth temperature, rather than at the sub-optimal growth temperature of $15^{\circ}C$.

• 생물환경조절학회지
• /
• 제26권4호
• /
• pp.310-316
• /
• 2017

방울토마토의 수경재배 중 붕소+칼슘+규소 및 칼슘+규소의 복합 엽면시비가 수확 후 품질과 MAP 저장 중 저장성에 미치는 영향을 알아보고자 본 연구를 실시하였다. 엽면시비한 방울 토마토('Unicorn')는 반숙과 상태에서 수확하여 산소투과성 필름으로 포장한 $5^{\circ}C$, $11^{\circ}C$, 그리고 $24^{\circ}C$에서 25일, 15일, 10일간 저장하였다. 붕소+칼슘+규소 복합처리한 방울토마토가 3가지 저장온도 모두에서 호흡과 에틸렌 발생이 억제되어 MAP 저장중 가장 낮은 생체중 감소와 가장 높은 외관상 품질을 보였다. 수확 후 조사한 방울토마토의 경도, 산도, 비타민 C 함량은 붕소+칼슘+규소 복합처리에서 가장 높았으며, 3가지 온도 모두에서 MAP 저장 후에도 모두 높게 유지되었다. 그러나 과피색, 라이코펜 함량과 당도는 수확 후에는 엽면시비 처리로 차이가 없었으나, 3가지 온도 모두 붕소+칼슘+규소 복합처리에서 가장 낮은 수치를 보였다. 이상의 결과로 볼 때 붕소+칼슘+규소 복합처리는 방울토마토의 수확후 생리 작용을 억제하고 경도, 산도, 비타민 C 함량을 높여 저장성을 향상시키는 것으로 판단되었다.

• 한국조경학회:학술대회논문집
• /
• 한국조경학회 1999년도 정기대회 및 학술논문발표회
• /
• pp.76-77
• /
• 1999

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2019년도 추계학술대회
• /
• pp.164-164
• /
• 2019

• 한국환경과학회지
• /
• 제27권8호
• /
• pp.631-639
• /
• 2018

A Cultivation method to minimize the damage caused by high temperatures was studied by investigating the effects of groundwater cooling treatment on the growth, yield, and quality of strawberries. In the groundwater cooling treatment, the daily average temperature of the rhizosphere was reduced from $26.9^{\circ}C$ to $24.9^{\circ}C$. The root length increased by 0.3-9.2 cm, depending on the cultivar and growth period. The leaf number, leaf area, leaf length, leaf diameter, and plant height also increased, especially in the cultivars 'Seolhyang' and 'Maehyang', resulting in higher fresh and dry weights. The number of fruit per plant increased from 7.7 to 12.5 in 'Seolhyang', and the fruit weight increased by 0.3 g in 'Seolhyang' and 1.3 g in 'Maehyang'. The fruit hardness increased, but no significant difference in fruit coloration was observed. The sugar content of the fruit was improved by $0.2-0.3^{\circ}Brix$. Therefore, groundwater cooling of the rhizosphere was effective in improving the growth and productivity of strawberries under abnormally high temperature conditions and can be considered a cost-efficient cooling system.

• The Plant Pathology Journal
• /
• 제36권5호
• /
• pp.418-427
• /
• 2020

Xanthomonas campestris pv. campestris (Xcc), the pathogen of black rot which is the most destructive disease of Brassica vegetables throughout the world. Here, we reported two novel sequence-characterized amplified region (SCAR) markers (i.e., XccR6-60 and XccR6-67) for the detection of Xcc race 6 via re-alignment of the complete genome sequences of Xcc races/strains/pathovars. The specificity of SCAR primer sets was verified by mean of PCR amplification using the genomic DNA template of Xcc races/strains/pathovars and two other plant infecting bacterial strains. The PCR result revealed that the XccR6-60 and XccR6-67 primer sets amplified 692-bp and 917-bp DNA fragments, respectively, specifically from race 6, while no visible amplification was detected in other samples. In addition, the SCAR primers were highly sensitive and can detect from a very low concentration of genomic DNA of Xcc race 6. However, the complete genome sequence of Xcc race 6 is not yet publicly available. Therefore, the cloning and sequencing of XccR6-60 and XccR6-67 fragments from race 6 provide more evidence of the specificity of these markers. These results indicated that the newly developed SCAR markers can successfully, effectively and rapidly detect Xcc race 6 from other Xcc races/strains/pathovars as well as other plant pathogenic bacteria. This is the first report for race-specific molecular markers for Xcc race 6.

• BMB Reports
• /
• 제40권3호
• /
• pp.419-425
• /
• 2007

In vitro directed evolution through DNA shuffling is a powerful molecular tool for creation of new biological phenotypes. E. coli $\beta$-galactosidase and $\beta$-glucuronidase are widely used, and their biological function, catalytic mechanism, and molecular structures are well characterized. We applied an in vitro directed evolution strategy through DNA shuffling and obtained five mutants named YG6764, YG6768, YG6769, YG6770 and YG6771 after two rounds of DNA shuffling and screening, which exhibited more $\beta$-glucuronidase activity than wild-type $\beta$-galactosidase. These variants had mutations at fourteen nucleic acid sites, resulting in changes in ten amino acids: S193N, T266A, Q267R, V411A, D448G, G466A, L527I, M543I, Q626R and Q951R. We expressed and purified those mutant proteins. Compared to the wild-type protein, five mutant proteins exhibited high $\beta$-glucuronidase activity. The comparison of molecular models of the mutated and wildtype enzymes revealed the relationship between protein function and structural modification.

• Journal of Plant Biotechnology
• /
• 제30권4호
• /
• pp.335-339
• /
• 2003

pSK109를 포함하고 있는 Agrobacterium LBA4004을 통하여 국화 'Shuho-no-chikara'에 LEAFY 유전자을 도입하였다. 엽절편체의 생존율은 kanamycin 10mg/L가 첨가된 배지에서 생존율 약 22%, 절편체의 재분화율이 10%정도였다. 그러나 kanamycin 20mg/L가 첨가된 배지에서는 엽절편은 약 5%정도 생존하였으나 전혀 재분화가 나타나지 않았다. 따라서 kanamycin 10mg/L를 1차 선발배지로, 20mg/L를 2차 선발배지로 선발하였다. Agrobacterium LBA4404와 국화 엽절편체를 3일간 공동배양 하는 것이 형질전환에 효과적이었다. LEAFY유전자가 삽입된 pSK109 vector를 포함한 Agrobacterium LBA4404의 형질전환 효율은 2차 선발까지 약 2.8% 였으며, Southern blot한 결과 0.13%만이 형질 전환체로 확인되었다. 형질 전환체 한계통은 포장에서 약 1주일 정도 조기개화하였고 화형은 정상이었다.