• Title/Summary/Keyword: Korean cucumber-hot pepper

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Detection of Virus in Fruit and Seed of Vegetables Using RT-PCR (RT-PCR에 의한 과채류 열매 및 종자의 바이러스 검정)

  • 최장경;김혜자;윤주연;박선정;김두욱;이상용
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.630-635
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    • 1998
  • Tobacco mosaic tobamovirus (TMV), cucumber mosaic cucumovirus (CMV), cucumber green mottle mosaic tobamovirus (CGMMV) and zucchini yellow mosaic potyvirus (ZYMV) from individual fruits and seeds of hot pepper and cucumber were detected by the reverse transcription-polymerase chain reaction (RT-PCR). The dilution end-points for RT-PCR in curde sap from TMV. and CMV - infected hot pepper leaves and CMV - and CGMMV-infected cucumber leaves were 10-5. However, the amount of PCR product obtained from preparation of ZYMV-infected cucumber leaf was 10-fold lower than those of CMV or CGMMV-infected cucumber leaves. In hot pepper, both TMV and CMV were detected in all parts of the fruit wall tissue, but the yields of PCR products in the fruit stalk and its surrounding tissues were higher than those of the end parts of the fruit. On the other hand, in cucumber fruit infected with CMV, CGMMV or ZYMV, the fruit wall tissue and seed located in both stalk and end parts showed higher yields of PCR products than those of intermediate parts. Of five viruses that were analysed, only TMV in hot pepper seed, and CGMMV and CMV in cucumber seed were detected in testa parts.

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Changes in the Components and Acceptability of Cucumber-Hot Pepper Pickles during Storage (오이고추 피클의 저장기간 중의 성분 및 기호도의 변화)

  • Jeong, Ji-Eun;Shin, Ji-Eun;Hwang, Kyung-Jun;Lee, Jae-Wook;Kim, Sun-Il
    • Korean journal of food and cookery science
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    • v.25 no.3
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    • pp.345-349
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    • 2009
  • The principle objective of this study was to determine the approximate composition of cucumber-hot pepper pickles under 3 conducted. In the stored cucumber-pepper samples, the approximate composition was characterized by a relative increase in protein content as compared to commercial cucumber-pepper samples, although other compositional components, such as ash and lipid contents, remained unchanged. Increases in storage time resulted in a reduction in the strength and hardness of the samples. Cucumber-pepper pickles stored for 15 days at a temperature of -4$^{\circ}$C evidenced the best overall acceptability in the sensory test.

Effects of Deep Sea Water Treatment on the Inhibition of Over-Growth of Plug Seedlings (해양심층수 처리가 육묘 시 도장억제에 미치는 영향)

  • Kang Won-He;Hong Sung-Yu
    • Korean Journal of Organic Agriculture
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    • v.14 no.1
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    • pp.85-95
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    • 2006
  • The investigation of the effect of the deep sea water in different concentration (0%, 10%, 20%, 30%, 40%) on the inhibition of growth, fresh weight, dry weight of the tomato, hot pepper, cucumber plug seedlings were conducted. As the concentration of the deep sea water was higher, the greater inhibition was noticed. The height were decreased at the rate of 8%, 25%, 32% in tomato, 9%, 26%, 27% in hot pepper, and 21%, 50%, 58% in cucumber plug seedlings as compare to local check. The fresh weights were decreased highly, as the concentration of the deep sea water increased. Tomato seedlings showed the decrease rate of 12%, 23%, 33% at above the ground part of plants, and 2%, 30%, 52% at under the ground parts. Likewise, Hot pepper seedlings showed the decrease rate of 1.1%, 5.7%, 15.4% at above the ground part of plants, and 22.1%, 25%, 47.1%, respectively at under the ground parts. The fresh weight of cucumber was decreased at the rate of 8.1%, 36%, 51% in the above the ground, and 6.2%, 11%, 65% at the under the ground parts. In tomato, hot pepper, and cucumber seedlings, the dry weight was decreased as the concentration of deep sea water was increased. Thirty percent of deep sea water treatment reduced the dry weight of the seedlings as half of the control seedlings. One of the important factors of the seedling quality is compactness. Compactness was highest at 20% deep sea water in all the three crop seedlings.

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Plant Growth Monitoring Using Thermography -Analysis of nutrient stress- (열영상을 이용한 작물 생장 감시 -영양분 스트레스 분석-)

  • 류관희;김기영;채희연
    • Journal of Biosystems Engineering
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    • v.25 no.4
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    • pp.293-300
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    • 2000
  • Automated greenhouse production system often require crop growth monitoring involving accurate quantification of plant physiological properties. Conventional methods are usually burdensome, inaccurate, and harmful to crops. A thermal image analysis system can accomplish rapid and accurate measurements of physiological-property changes of stressed crops. In this research a thermal imaging system was used to measure the leaf-temperature changes of several crops according to nutrient stresses. Thermal images were obtained from lettuce, cucumber, and pepper plants. Plants were placed in growth chamber to provide relatively constant growth environment. Results showed that there were significant differences in the temperature of stressed plants and non-stressed plants. In a case of the both N deficiency and excess, the leaf temperatures of cucumber were $2^{\circ}C$ lower than controlled temperature. The leaf temperature of cucumber was $2^{\circ}C$ lower than controlled temperature only when it was under N excess stress. For the potassium deficiency or excess stress, the leaf temperaures of cucumber and hot pepper were $2^{\circ}C$ lower than controls, respectively. The phosphorous deficiency stress dropped the leaf temperatures of cucumber and hot pepper $2^{\circ}C$ and $1.5^{\circ}C$ below than controls. However, the leaf temperature of lettuce did not change. It was possible to detect the changes in leaf temperature by infrared thermography when subjected to nutrition stress. Since the changes in leaf temperatures were different each other for plants and kinds of stresses, however, it is necessary to add a nutrient measurement system to a plant-growth monitoring system using thermography.

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Effect on the Inhibition of Over-growth of Plug Seedling by Triazole-type Growth Regulator Treatment (Triazole계 생장조절제 처리가 공정육묘의 도장억제에 미치는 영향)

  • Zhang, Cheng-Hao;Chun, Ik-Jo;Park, Yong-Chul;Kim, Il-Seop
    • Journal of Bio-Environment Control
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    • v.12 no.3
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    • pp.139-146
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    • 2003
  • This study was conducted to investigate the effects on the over-growth inhibition of fruit vegetables (tomato, cucumber, and hot pepper) seedling by the treatment of triazole-typechemicals and to select the most effective chemicals for the over-growth inhibition, and to investigate suitabla concentration treatment and times of the newly selected chemical. The triazole-type chemicals inhibited stem height more, the more the concentration is increased in any crops. The diniconazole showed the highest dwarfing ration, and the tebuconazole and hexaconazole are also good for dwarfing ratio. The diniconazole decreased the plant height of cucumber, tomato, and hot pepper as the concentration is increased. In cucumber, thc dwarfing ratio was 30% in the 5 mg${\cdot}L^{-1}$ treatment, and was 60% in thc l00 mg${\cdot}L^{-1}$ treatment. In tomato and hot pepper, the dwarfing ratio was 30% in the 25 mg${\cdot}L^{-1}$ treatment in any times. In diniconazole treatment, the cucumber and tomato should have 5 mg${\cdot}L^{-1}$ at the lst growth stage and the hot pepper should have 5 mg${\cdot}L^{-1}$ at the 2nd growth stage to obtain good seedlings.

Developing descriptive analysis protocol for gochujang: establishing optimal palate cleanser (고추장 묘사분석을 위한 프로토콜 개발: 입가심물질 중심으로)

  • Lee, Eun-Hye;Chung, Seo-Jin;Yu, Seon-Mi;Han, Kui-Jeong
    • Korean journal of food and cookery science
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    • v.29 no.5
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    • pp.489-500
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    • 2013
  • This study was conducted to establish an effective palate cleanser when conducting descriptive analysis for gochujang products. In addition, descriptive analysis procedure for gochujang products was optimized. A generic descriptive analysis was performed on 4 types of gochujang samples varying in hot and spicy levels. The sensory attributes developed were 9 odors, 13 flavors, 4 texture and mouth feel attributes, and 4 appearance attributes. In order to select an effective palate cleanser for gochujang, 5 types of cleansers were tested (water, water+bread, water+cucumber, water+milk, water+cracker). Correct answering rate, significant numbers of product effect on sensory attributes, and the mean hot and spicy intensity values were considered to select the optimal palate cleanser. Results showed that as the hot & spicy level increased, red pepper odor and flavor significantly increased whereas umami taste intensity decreased. When comparing the efficiencies of various palate cleanser, the correct answer rates were the highest when warm water was used with either cucumber or cracker. Additionally, the attribute intensities were better differentiated between gochujang samples when cracker, white wheat bread, or cucumber were used. Overall, warm water with cucumber or cracker were shown to be the most effective cleansers.

Physicochemical and Sensory Characteristics of Cucumber Salad Using Spicy Hot Flavor Graded Gochujang (매운맛 등급화 고추장으로 제조한 오이생채의 이화학적 관능적 특성)

  • Lee, In-Seon
    • Korean journal of food and cookery science
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    • v.31 no.3
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    • pp.255-263
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    • 2015
  • This study investigated the spicy hot flavor related quality characteristics of cucumber salad prepared using spicy hot flavor graded gochujang that had been stored for 60 and 120 days. The results showed that the pH of both the 60-day and 120-day fermented gochujang was significantly higher in the cap-200 sample (p<0.001). In contrast to the results on pH, the acidity of both the 60-day and 120-day fermented gochujang was found significantly lower in the cap-200 sample (p<0.001). Measurements of salinity were found to be significantly lower in the sample with higher capsaicin content than in the sample with lower capsaicin content. The sugar content was found to be generally higher in the sample with the 120-day fermented gochujang than that with the 60-day fermented gochujang. Lightness (L) was higher in the sample with a lower capsaicin content than in the sample with a higher capsaicin content. The redness (a) was found to be significantly higher in the cap-200 sample than in other samples (p<0.001). The values of the L, a, and b tended to be lower in the sample with the 60-day fermented gochujang than in the sample with the 120-day fermented gochujang. The hardness of the cucumber salad was found to be lowest in the 120-day fermented sample with the cap-200 gochujang, which had the highest capsaicin content (p<0.05). The results of the sensory evaluation of the cucumber salads showed that the spiciness was stronger with the higher capsaicin content (p<0.001), and approximately one-level higher than the spiciness rating of hot pepper. The results of a key ingredient analysis showed that the sample with high capsaicin content was classified as having a relatively hot spicy aroma and flavor, and strong stinging pain, and the sample with the low capsaicin content was classified as having a salty aroma and a dark appearance.

Evaluation of Hot Water Treatment for Disinfection of Vegetable Seeds for Organic Farming (채소 종자별 온탕침지 종자소독 효과검정)

  • Lee, Ji-Hyun;Shen, Shun-Shan;Park, Yong-Ju;Ryu, Kyung-Yul;Jee, Hyeong-Jin
    • Research in Plant Disease
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    • v.13 no.3
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    • pp.157-163
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    • 2007
  • Hot water treatment that is the most appropriate seed disinfection method for organic vegetable farming was evaluated in this study. Among the leafy vegetable seeds lettuce that was the most sensitive to hot water was suitable to treat at $45^{\circ}C$ for 25 min, while Chinese cabbage and radish seeds were optimally treated at $50^{\circ}C$ for 25 min. The treatments resulted in similar or higher seed germination rate than non-treated seeds and promoted plant growth. In addition, fungi such as Alternaria, Aspergillus, Penicillium, or Mucor grown on the seeds were suppressed over 90% and the bacterial growth on lettuce seeds reduced 98.5% by the treatment. Among the fruit vegetable seeds pumpkin that was vulnerable to hot water was suitable to treat at $50^{\circ}C$ for 15 min, while cucumber and hot pepper seeds revealed optimum treatment at $50^{\circ}C$ for 25 min as chinese cabbage and radish. The treatment also showed similar or higher seed germination rate and growth than non-treated seeds. Furthermore, fungi such as Rhizopus, Aspergillus, Penicillium or Mucor grown on the seeds reduced from 72.0% to 95.4%. The bacterial growth on cucumber and red pepper seeds was suppressed from 65.5% to 86.0% by the treatment. Results indicated that the hot water treatment is practical for disinfection of organic vegetable seeds and the optimum temperature and soaking time varied among the seeds.

Analysis of Thermotolerance in Hot Pepper Using the Antiserum Against Carrot HSP17

  • Hwang, Eun-Young;Hwang, Cheol-Ho;Yoo, Il-Woong
    • Journal of Plant Biotechnology
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    • v.3 no.1
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    • pp.7-12
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    • 2001
  • An antiserum against the carrot HSP17 (17 KDa heat shock protein) was raised using the HSP17 purified after being expressed in a recombinant E.coli in order to develop an assay system for thermotolerance in crops. The DCHsp17.7 including the coding sequence corresponding to a carrot HSP17 protein was recombined within pET-32(b) vector and achieved a maximum expression in 4 hours after an induction in E.coli. The purified DCHsp17.7 was used as an antigen to generate the corresponding antibody. The polyclonal antiserum was confirmed for it's specificity only to the low molecular weight (1mw) HSP. Besides, the possibilities to use the antiserum to interact with 1mwHSPs from other plants such as rice, cucumber, tomato, and hot pepper were examined to be plausible. To reveal any specific correlation between the amounts of 1mwHSP expressed upon HS conditions and an acquisition of thermotolerance two different approaches have been applied. first, it has been shown that only the pre-HS conditions inducing the synthesis of HSP17 allowed for the seedlings to achieve an thermotolerance and to survive the following lethal condition. Second, a western analysis using 15 different collected lines of hot peppers was performed to distinguish each other in terms of the amount of 1mwHSP. The results indicated that all 14 hot pepper lines were able to synthesize HSPs in response to an exposure to HS conditions and the amounts of the proteins synthesized at different HS temperatures were variable among the lines. There are several different patterns of 1mwHSP synthesized as a function of temperature increase observed and their correlation to physiological aspects of thermotolerance remains to be analyzed.

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An Investigation of the Hazards Associated with Cucumber and Hot Pepper Cultivation Areas to Establish a Good Agricultural Practices (GAP) Model (오이와 고추생산 환경에서의 GAP 모델 개발을 위한 위해요소 조사)

  • Shim, Won-Bo;Lee, Chae-Won;Jeong, Myeong-Jin;Kim, Jeong-Sook;Ryu, Jae-Gee;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.46 no.1
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    • pp.108-114
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    • 2014
  • To analyze the hazards associated with cucumber and hot pepper cultivation areas, a total of 72 samples were obtained and tested to detect the presence of biological (sanitary indicative, pathogenic bacteria and fungi) and chemical hazards (heavy metals and pesticide residues). The levels of sanitary indicative bacteria (aerobic plate counts and coliforms) and fungi were ND-7.2 and ND-4.8 log CFU/(g, mL, hand, or $100cm^2$) in cucumber cultivation areas, and ND-6.8 and 0.4-5.3 log CFU/(g, mL, hand, or $100cm^2$) in hot pepper cultivation areas. More specifically, the soil of hot pepper cultivation areas was contaminated with coliforms at a maximum level of 5.6 log CFU/g. Staphylococcus aureus was detected only in glove samples at a level of 1.4 log CFU/$100cm^2$ and Bacillus cereus was detected in the majority of samples at a level of ND-4.8 log CFU/(g, mL, hand, or $100cm^2$). Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp. were not detected. Heavy metal (Zn, Cu, Ni, Pb, and Hg) chemical hazards were detected at levels lower than the regulation limit. Residual insecticides were not detected in cucumbers; however, hexaconazole was detected at a level of 0.016 mg/kg (maximum residue limit: 0.3 mg/kg) in hot peppers.