• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1239-1248
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• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Journal of Pharmaceutical Investigation
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• v.27 no.4
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• pp.287-293
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• 1997

To make a stable o/w emulsion, the effects of egg lecithin as an emulsifier and polyvinylpyrrolidone (PVP) as an auxiliary emulsifier on the physical stability of emulsion were investigated. The oil-in-water emulsion system was manufactured by microfluidizer and evaluated the physical stability. Average particle size and size distribution of emulsion was measured by dynamic light scattering analyzer and interfacial tension was measured. From the interfacial tension tested, critical micelle concentration of the egg lecithin was 0.1 %w/v and optimal concentration for the preparation of emulsion was 1.0 %w/v. The mean particle size was about $0.2\;{\mu}m$ which was suitable for injections. The short-term accelerated stability studies were conducted by centrifugation, freeze-thaw method and shaking of the emulsion samples. The addition of PVP was caused the reduction in the particle size and improved the physical stability of emulsion. These results suggested that a mixed interfacial film comprising the egg lecithin and PVP was formed at the o/w interface and it was effective in preventing phase separation under thermic or mechanical stress. We used antineoplaston A10 (A10) as a model drug which is peptide and amino acid derivative having a action to the living organism against the development of neoplastic growth by a nonimmunological progress. It has a poor solubility in water and there may be a difficulty in formulation of A10. Emulsion formulation study about A10 was performed. Solubility of A10 in emulsion was about five times as high as that in water. From the results of solubility and partition coefficient, almost A10 molecules in o/w emulsion exist in the interface between oil and water.

• The Journal of Engineering Geology
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• v.25 no.2
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• pp.237-250
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• 2015

Underground structures such as a tunnel have been considered as safer than structures on the ground during earthquake. However, severe damages of underground structures occurred at subway tunnel during 1995 Kobe Earthquake and such damages are gradually increased. In this study, a dynamic behavior of a cut and cover tunnel surrounded by weathered soils is investigated using Mohr-Coulomb Model. Parametric study was carried out for boundary conditions, tensile strength, and earthquake magnitudes. The results of numerical analyses in terms of ground deformations and stresses acting on the lining were quite dependent on the side boundary condition (free or fix conditions) and tensile strength of surrounding soils. The ground was deformed upward at the end of earthquake when the side boundary condition was fixed, whereas residual deformations were not predicted when it was free. When the tensile strength of a soil was set to the same as its cohesion, residual deformation was less than 1cm, regardless of side boundary conditions or input accelerations. In addition to that, stress conditions at the maximum deformation and end of earthquake were within an allowable range and considered as safe. Proper boundary conditions and material properties such as tensile strength are quite important because they may significantly impact on the results of dynamic analyses.

• Journal of Internet Computing and Services
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• v.10 no.1
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• pp.145-158
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• 2009

No other change in Kora has offered gredter chalenges and opportunities than the emergence of the Internet and its related Information Technology. In the technology for accessing the ever growing wave of information. The quality of the silver generation is increasingly difficult to achieve because of the complexities of life in today's ICT(Information & Comunication Technology) dependent society, Life is more interesting when one knows what is going on, what opportunities exist, and when alternatives to current practices can be discovered and utilized with ease. The purpose of this study is to identify the determinants of attitude and planned behavior toward using internent for the quality of life. This study employ the intention to use internet, perceived ease of use, perceived usefuiness, self-efficacy, stress factor, generation gap, and income as major research variables, and collected 288 survey responses from senior citizen over 55. The results indicate that the perceived usefuiness and ease of use influence the intention to use internet, and that the self-efficacy, the ease of use and generation gap has some impact on the attitude, but the stree factor and the income level do not appear significant to the attitude. It was also found that the self-efficacy and the income level determine the level of ease of use. In addition, some useful suggestions concerning the continuing education program for silver generations are presented.

• Clinical and Experimental Pediatrics
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• v.52 no.9
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• pp.1005-1014
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• 2009

Purpose:Epilepsy of child may cause high level of psychosocial difficulties for parents including stigmatization and stress and therefore worsen their quality of life (QOL). The purpose of this study was to evaluate the mediating effect of perceived stigma against epilepsy on QOL among parents with epileptic child. Methods:Two hundred and sixty parents of epileptic child recruited from five separated university hospital child neurology clinics specializing in epilepsy completed a demographic questionnaire, a medical questionnaire, a perceived stigma questionnaire and the Ro's Quality of Life Inventory, a popular tool for evaluating QOL of adults in Korea which is composed of 6 domains and 47 questions. Data was analyzed with SPSS 14.0 program using frequency analysis and descriptive analysis and with AMOS 7.0 program using Structural Equation Model (SEM) analysis. Results:The level of parents' QOL was relatively fair. SEM analysis on the quality of life level of parents showed that all variables (especially having religion, the monthly income, employment state, age of child, the leisure time, the perceived stigma level against epilepsy, and seizure frequency) directly affect the quality of life level of parents and that disease-related variables also affect the quality of life level of parents indirectly through the mediating factor (the perceived stigma), where the indirect effect is large with the existence of combined disabilities. The total effect on the quality of life level of parents is large with the existence of combined disabilities, the perceived stigma level, the leisure time, having religion, and the monthly income. Conclusion:The results of this study indicated that many factors including parents' perceived stigma may affect the quality of life of parents with epileptic children directly, and that some disease-related factors may affect indirectly through a mediating factor, the perceived stigma. Multidisciplinary collaboration would be imperative for their welfare.

• Journal of Biomedical Engineering Research
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• v.38 no.1
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• pp.9-15
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• 2017

Recently, flexible cages have been introduced in an attempt to absorb and reduce the abnormal load transfer along the anterior parts of the spine. They are designed to be used with the pedicle screw systems to allow some mobility at the index level while containing ROM at the adjacent level. In this study, a finite element (FE) study was performed to assess biomechanical efficacies of the flexible cage when combined with pedicle screws with flexible rods. The post-operated models were constructed by modifying the L4-5 of a previously-validated 3-D FE model of the intact lumbar spine (L2-S1): (1) Type 1, flexible cage only; (2) Type 2, pedicle screws with flexible rods; (3) Type 3, interbody fusion cage plus pedicle screws with rigid rods; (4) Type 4, interbody fusion cage plus Type 2; (5) Type 5, Type 1 plus Type 2. Flexion/extension of 10 Nm with a compressive follower load of 400N was applied. As compared to the Type 3 (62~65%) and Type 4 (59~62%), Type 5 (53~55%) was able to limit the motion at the operated level effectively, despite moderate reduction at the adjacent level. It was also able to shift the load back to the anterior portions of the spine thus relieving excessively high posterior load transfer and to reduce stress on the endplate by absorbing the load with its flexible shape design features. The likelihood of component failure of flexble cage remained less than 30% regardless of loading conditions when combined with pedicle screws with flexible rods. Our study demonstrated that flexible cages when combined with posterior dynamic system may help reduce subsidence of cage and degeneration process at the adjacent levels while effectively providing stability at the operated level.

• Journal of Navigation and Port Research
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• v.30 no.6 s.112
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• pp.439-446
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• 2006

In ship structures many of the structural plates have cutouts, for example, at inner bottom structure, girder, upper deck hatch, floor and dia-frame etc. In the case where a plate has a cutout it experiences reduced buckling and ultimate strength and at the same time the in-plane stress under compressive load produced by hull girder bending will be redistributed In general, actual ship structure adopted reinforcement of stiffener around the cutout in order to preventing from buckling so it need to examine a buckling and ultimate strength behaviour considering a cutout because In many ship yards used class rule for calculating buckling strength but it is difficult to evaluate perforate stiffened plate with random size. In the present paper, we investigated several kinds of perforated stiffened model from actual ship and then was performed finite element series analysis varying the cutout ratio, web height, thickness and type of cross-section using commercial FEA program(ANSYS) under compressive load.

• Journal of Acupuncture Research
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• v.29 no.1
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• pp.89-101
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• 2012

Objectives : The purpose of this study was find out the therapeutic effects of its exclusive use on the rat with allergic rhinitis. Materials and Methods : Thirty Sprague-Dawley rats were divided into three group : normal group, control group and sample group. To induce the allergic rhinitis in control group and sample group, rats were sensitized intraperitoneally with 0.1% ovalumin solution 3 times at intervals of 1 week. Then intranasal sensitization was performed by diffusing 0.1% ovalumin solution 3 times at intervals of 2 days. After that time, rats in the sample group were administered by $Yonghyang$($LI_{20}$) subcutaneously to treat the inflammation. Results : 1. The anti-oxidant effects of $Hwangryunhaedok-tang$ extract was dose-dependantly increased. 2. The RAW 264.7 cells were treated with LPS for 1 hours prior to the addition of indicated concentrations ($0.4,-1.0mg/m{\ell}$) of HHT, and the cells were further incubated for 24 hours. The LPS-induced iNOS mRNA expression and NO production were dose-dependantly decreased in HHT treated RAW 264.7 cells. 3. The number of eosinophil in HP noticeably decreased than CON and this decrease had probability. The infiltration of eosinophil in HP noticeably decreased than CON. 4. The damaged mucosa as disruption of cilia in respiratory cell and vacant mucose secreting cell were increased CON, but HP same as normal configuration. Decrease of PAS positive cell were shown in CON, but goblet cell occupied with neutral mucous were shown in HP. Decrease of mucosal stress(HSP70). Decrease of perennial sign(PPAR-${\gamma}$). Decrease of icthing and sneezing intricate neurotransmitter-(substance P). 5. The anti-inflammation of HHT pharmacopuncture for AR caused mucosa comes to result as belows. Decrease of pre-inflammation cytokine(TNF-${\alpha}$). Decrease of transcription factor (NF-${\kappa}B$ p65). Decrease of transcription factor inhibitor(p-$I{\kappa}B$). Decrease of inflammation cytokine(iNOS). Conclusions : The results may suggest that administration treatment using $Hwangryunhaedok-tang$ pharmacopucnture decreases the inflammatory response on an animal model with allergic rhinitis.

• Geotechnical Engineering
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• v.14 no.6
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• pp.93-112
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• 1998

In the present study, 2-D consolidation theory of the dredged clay by means of the horizontal drain method is proposed. The horizontal drain method to install the drains such as plastic drain board within the dredged clay is a soil improvement method to accelerate the consolidation by expelling pore water in the vertical direction along the horizontal drains. Based on the finite strain consolidation theory by Gibson et al., the partial differential equation of 2-D consolidation due to the horizontal drain is derived. The consolidation due to the horizontal drain can be illustrated from combined self-weight consolidation effect and consolidation effect by horizontal drains. For the prediction of consolidation settlement and degree of consolidation numerical analysis is suggested on the basis of Dufort-Frankel finite differential algorithm. Also, the analytical procedures proposed in this study are verified by the model tests, and the predictions of the consolidation settlement and degree of consolidation are compared with the results obtained from the tests for the dredged clay gathering at Siwha site in Ansan, Korea. For the predictions, the relationship void ratio vs effective stress and the relationship permeability vs void ratio of the dredged clay are obtained from the odometer tests. Additionally, the parametric study for consolidation settlement by variations of design parameters related with horizontal drain method is carried out. Based on the results of the parametric study, design .charts for the preliminary design are also proposed.

• Journal of Families and Better Life
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• v.27 no.1
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• pp.73-88
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• 2009

The purpose of this study was to investigate the influence of the factors such as self-esteem, social supports, socio-economic status of parents, degree of disability in child, stress coping, religion, and sports.exercise on the qualities of lives in mothers with disabled children. Data were collect from 45 institutions(schools from kindergarten to senior-high, special education institutes, and social welfare institutions) by self-report questionnaire, and the subjects of the study were 386 mothers in Seoul whose children were attending the schools or institutions. Findings according to socio-demographic variables are as follows: 1. Mothers with disabled children in this study thought their quality of life as average degree. 2. The younger the mothers and children, the higher the quality of life was. 3. The higher the educational level and monthly income, the higher the quality of life was. 4. There was no statistically significant difference between the existence and nonexistence of spouse, and between the existence and nonexistence of religion as well. Findings analyzed by multiple regression are as follows: 1. Among 7 independent variables 'self-esteem'(${\beta}$=.49, p<.001), 'social supports'(${\beta}$=.15, p<.01), and 'socio-economic status'(${\beta}$=.11, p<.05) were found to be statistically significant in quality of life in mothers, while the rests were found not significant. 2. 'Self-esteem' was found to be the most influencing variable, meaning that the psychological factor such as self-esteem is more important than environmental factors in qualities of lives in mothers. 3. The total amount of explanation of the model was Adjusted R square=.301, so that it can be said about 30% can be explained among total variance of the qualities of lives in mothers with disabled children. Conclusions and recommendations based on the results above are: First, it is strongly recommended to conduct programs promoting self-esteem in mothers with disabled children, and to include 'self-esteem' hereafter in the studies related to qualities of lives in mothers with disabled children. Second, comprehensive and practical countermeasures should to be formulated to back up expenses for education and medical care, purchasing relative tools and equipments, plus helping to find jobs.