• Journal of Animal Environmental Science
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• v.11 no.1
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• pp.45-54
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• 2005

A field survey was conducted to determine the concentration of odor compounds from pig buildings and that were 20 meters within the boundary area. The odor compounds were measured from large, medium and small farms with enclosed and open housing systems and slurry and sawdust manure fermentation treatment methods. Among the odor compounds investigated, ammonia ($NH_3$) had the highest concentration at 0.9 ${\~}$ 21.0 ppm followed by Hydrogen Sulfide($H_2S$) with a wide variation concentration of 51.9 ${\~}$ 6,712.4 pub, Uethylmercaptan($CH_3SH$) with non-detectable (N. D.) ${\~}$ 12.9 ppb, Dimethylsulphide($(CH_3)_2S$), with N. D. ${\~}$ 5.2 ppb and Dimethyldisulphide($(CH_3)_2S_2$) with N. D. ${\~}$ 2.6 ppb. Considering the prevailing wind direction and air velocity ranging from 0.23 to 0.73 m/s within the boundary area, the odorous matters; $NH_3$, $H_2S$, $CH_3SH$, $(CH_3)_2S_2$ and $(CH_3)_2S$ were 0.2${\~}$4.5 ppm, 0.01 ${\~}$0.06 ppb, N. D. ${\~}$0.009ppb, N. D.${\~}$0.002ppb and N. D. for $(CH_3)_2S$ respectively. These findings suggested that the Odor compounds $(CH_3)_2S_2$ had the lower detection in the boundary area whilst $(CH_3)_2S$ had no detection level within a 20-meter distance only. However, with these results odor compounds from pig buildings has to be further investigated under more controlled environmental factors.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.4
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• pp.232-239
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• 2023

This study was to evaluate the values of Korean native sweet sorghum as a new feed crop for ruminants. Sweet sorghum was the Muan native species (Bioenergy Crop Research Center, National Institute of Crop Science), and cultivated from May to October 2021 at Sangji University (Wonju-si, Gangwon-do, Korea). There were a non-treated group (Con), a recommended amount treatment (RD) and a treatment with double the recommended amount (Double RD) by an oil cake fertilizer. Plant height was measured at weekly intervals for 12 weeks after planting sweet sorghum seedlings, and was a significant difference in the order of Double RD, followed by RD and Con in 7 weeks (p<0.05). Feed values and sugar contents were measured in 7, 9, and 11 weeks. Crude protein of Double RD was higher than that of the other treatments in 7 and 9 weeks (p<0.05). Crude fat was higher at Double RD than the other one in 9 weeks (p<0.05). ADF and NDF of Double RD were higher than the other one (p<0.05). When it was compared to corn and sudangrass hybrids grown on farms, Crude protein was lower in sweet sorghum than other crops (p<0.05), and crude fat was higher in sweet sorghum than corn (p<0.05). Crude fiber, ADF and NDF were higher in sweet sorghum compared to corn and sudangrass (p<0.05). The sugar contents of sweet sorghum were 4.07 ± 0.12~7.63 ± 0.21 brix, and showed higher than corn and sudangrass hybrid (p<0.05). The rumen in situ digestibility of sweet sorghum was 30.73~38.13% at the 9th and 11th weeks, and showed higher than that of corn and sudangrass hybrids (p<0.05). Therefore, it is considered that Korean native sweet sorghum has sufficient value as a new forage crop for ruminants, and good value as yield, nutrients and digestibility, when the grass height is 273.33~332.50 cm.

• Korean Journal of Veterinary Service
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• v.33 no.3
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• pp.275-285
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• 2010

This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of gal gun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1170-1176
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• 2015

Ginsenosides, the major active component of ginseng, are traditionally used to treat various diseases, including cancer, inflammation, and obesity. Among these, compound K (CK), an intestinal bacterial metabolite of the ginsenosides Rb₁, Rb₂, and Rc from Bacteroides JY-6, is reported to inhibit cancer cell growth by inducing cell-cycle arrest or cell death, including apoptosis and necrosis. However, the precise effect of CK on breast cancer cells remains unclear. MCF-7 cells were treated with CK ($0-70{\mu}M$) for 24 or 48 h. Cell proliferation and death were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Changes in downstream signaling molecules involved in cell death, including glycogen synthase kinase $3\beta$ ($GSK3\beta$), $GSK3\beta$, $\beta$-catenin, and cyclin D1, were analyzed by western blot assay. To block $GSK3\beta$ signaling, MCF-7 cells were pretreated with $GSK3\beta$ inhibitors 1 h prior to CK treatment. Cell death and the expression of $\beta$-catenin and cyclin D1 were then examined. CK dose- and time-dependently inhibited MCF-7 cell proliferation. Interestingly, CK induced programmed necrosis, but not apoptosis, via the $GSK3\beta$ signaling pathway in MCF-7 cells. CK inhibited $GSK3\beta$ phosphorylation, thereby suppressing the expression of $\beta$-catenin and cyclin D1. Our results suggest that CK induces programmed necrosis in MCF-7 breast cancer cells via the $GSK3\beta$ signaling pathway.

• Journal of Animal Environmental Science
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• v.9 no.2
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• pp.103-112
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• 2003

In this study, the distributions of internal climates such as air temperature, humidity, dust, ammonia gas, and air velocity were systematically measured at a mechanically ventilated broiler houses during summer season, with local weather data. The analysis was focused on the suitability, stability, and uniformity of internal climate, resulting in serious stress on chickens and decrease of productivity In the mechanically ventilated broiler house, the difference between measured and recommended air temperatures(suitability) was 10.4C in maximum during the summer time. The difference of air temperature in the house between day and night was $8.7^{\circ}C$ in maximum. And maximal hourly range of internal air temperature at 0.4m height from the floor was $3.7^{\circ}C$ suggesting it maintained thermal uniformity in the broiler house. The $NH_3$ and dust concentrations were pretty low because ventilation was fully performed. The air speed at chicken location was measured 2.2m/s and 1.7m/s, respectively without and with chicken existence.

• Korean Journal of Environmental Biology
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• v.40 no.1
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• pp.25-53
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• 2022

In this study, trends in research methods and topics of seabird and marine fish migration were examined. Based on the framework of existing animal migration studies, future research directions were proposed in relation to the migration of seabirds and fish. In terms of research methodology, with the development of science and technology, tracking techniques using radio telemetry, acoustic telemetry, RFID (radio-frequency identification), satellite tracking, and geolocators are widely used to study seabird and fish migration. Research is also conducted indirectly through a population survey and the analysis of substances in the body. Research contents are largely classified into extrinsic factors that affect migration(such as environmental variables and interspecific competition), intrinsic factors such as hormones, anthropogenic activities including fishery and offshore wind farm, and the effect of global climate change. In future studies, physiological factors that influence or cause migration and dispersal should be identified concerning intrinsic factors. For the analysis of migration ability, it is necessary to study effects of changes in the magnetic field on the migration ability of seabirds and fish, interspecific differences in spatiotemporal migration ability, and factors that influence the migration success rate. Regarding extrinsic factors, research studies on effects of anthropogenic disturbances such as fishery and offshore wind farm and global climate change on the migration and dispersal patterns of marine animals are needed. Finally, integrated studies on the migration of seabirds and fish directly or indirectly affecting each other in various ecological aspects are required.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.509-514
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• 2001

Effects of conjugated linoleic acid (CLA), known as an effective anticarcinogen in several animal models, on the tilapia were investigated. The CLA was made from safflower oil by alkaline isomerization method. Isomers in CLA such as cis-9, trans-11 and trans-10, cis-12 occupied over $80\%$, and other isomers was below $20\%$. In experiment, 250 fishes (average weight is 32 g) were divided into 15 fishes per five treatment and triplicate group for 8 weeks: control, $1\%$ CLA, $2.5\%$ CLA, $5.0\%$ CLA, and $10\%$ CLA diets. Daily growth rate and feed coefficiency were measured every week. The most effective diet for the growth rate and feed coefficiency of tilapia was $1.0\%$ CLA diet group. Every two weeks, sampled and determined the contents of CLA in the muscle and liver, After 8 weeks, $1.0\%$ and $10.0\%$ of CLA fed group accumulated the CLA as 41.3 and 180.9 mg/g of fat in their muscle respectively, Also, n-9 and n-3 fatty acid (FA) compositions were almost not changed in the muscle and liver. But n-6 fatty acid was changed according to the contents of fed CLA. The $1.0\%$ CLA fed group was shown the highest contents of n-6 FA and the $10.0\%$ CLA group was shown the lowest contents of n-6 FA.

• Korean Journal of Environment and Ecology
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• v.19 no.3
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• pp.279-286
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• 2005

This study was conducted to analyze the changing pattern of the bird community after the construction of artificial wetland at Sihwah lake from may 2000 to January 2002. Total seventy seven bird species were recorded at Sihwa artificial wetland area during the survey. Number of the bird species and individuals were increased in second year than first survey year. Especially shorebirds and raptors were more increased than other groups. Long-term and continuous monitoring of bird community would be needed to clarify the reasons of increasing pattern of bird species and individuals in artificial wetlands of Sihwa lake. Until now, this area has been considered as suitable habitat for dabbling ducks than shore birds and has simple habitat environment consisting of open water surface and reed beds. Therefore, we suggest the follows for creation of diverse habitat types: 1) seasonal water-level manipulation 2) management of diverse aquatic plants and 3) creation of diverse land cover; sandy fields, gravelly fields, grasslands, etc.

• Journal of Animal Environmental Science
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• v.12 no.3
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• pp.141-150
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• 2006

To optimise the efficient use of nutrients in pig slurry is to cultivate friendly environmental crops. This field survey is to investigate the actual conditions of pig slurry utilization for cultivation of crops in the agricultural farm, based on the survey for 407 selected farms in 9 provinces included 78 counties in Korea. The results obtained in this survey were summarized as follow ; The motive which came to use pig slurry in the agricultural farm were production of friendly environmental crops (29.7%), economy of chemical fertilizer (25.1%), spontaneously (19.2%), inducement of neighboring farmhouse (16.0%), increase of soil fertility (9.3%), and the others (0.7%), respectively. The proportions of pig slurry application land were 56.5% for.ice paddy, 22.6% for dry field, 13.3% for orchard, 4.4% for controlled agriculture and 3.2% for other, respectively. The number of times of pig slurry utilization per year were once (48.9%), twice (31.9%), thrice (14.0%), and the others (5.2%), respectively. The controversial points of pig slurry utilization were malodor (54.1%), insufficiency of spread equipment (22.1%), inconvenience (14.5%), over application (3.4%), over cost (2.9%), heavy metal (1.7%), sanitation (1.0%) and the other (0.2%), respectively. The results indicated that pig slurry could be used as fertilizer source of friendly environmental crops, but further studies are needed to determine the application method and value of the pig slurry for crop cultivation.

• The Korean Journal of Physiology and Pharmacology
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• v.28 no.4
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• pp.313-322
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• 2024

Mutations within the SCN5A gene, which encodes the α-subunit 5 (Na_V1.5) of the voltage-gated Na⁺ channel, have been linked to three distinct cardiac arrhythmia disorders: long QT syndrome type 3, Brugada syndrome (BrS), and cardiac conduction disorder. In this study, we have identified novel missense mutations (p.A385T/R504T) within SCN5A in a patient exhibiting overlap arrhythmia phenotypes. This study aims to elucidate the functional consequences of SCN5A mutants (p.A385T/R504T) to understand the clinical phenotypes. Whole-cell patch-clamp technique was used to analyze the Na_V1.5 current (I_Na) in HEK293 cells transfected with the wild-type and mutant SCN5A with or without SCN1B co-expression. The amplitude of I_Na was not altered in mutant SCN5A (p.A385T/R504T) alone. Furthermore, a rightward shift of the voltage-dependent inactivation and faster recovery from inactivation was observed, suggesting a gain-of-function state. Intriguingly, the co-expression of SCN1B with p.A385T/R504T revealed significant reduction of I_Na and slower recovery from inactivation, consistent with the loss-of-function in Na⁺ channels. The SCN1B dependent reduction of I_Na was also observed in a single mutation p.R504T, but p.A385T co-expressed with SCN1B showed no reduction. In contrast, the slower recovery from inactivation with SCN1B was observed in A385T while not in R504T. The expression of SCN1B is indispensable for the electrophysiological phenotype of BrS with the novel double mutations; p.A385T and p.R504T contributed to the slower recovery from inactivation and reduced current density of Na_V1.5, respectively.