• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.191-191
• /
• 2022

Heterostyly continues to fascinate evolutionary biologists interested in heredity, evolution, breeding, and adaptive function. Polymorphism demonstrates how simply inherited developmental changes in the location of plant sexual associations can have important consequences for population pollination and mating biology. In contrast to homozygous self incompatibility, only a small number of mating phenotypes can be maintained in the population because insect pollinators have limitations in achieving multiple segregation sites for pollen deposition. Field studies of pollen tube growth have shown that reciprocal style-stamen polymorphisms function to increase the capacity of insect-mediated cross-pollination. The genetic pattern of style morphs is well established in various taxa, but despite recent advances, the identity, number, and structure of the genes controlling the heteromorphic syndrome have been poorly elucidated. The phenomenon of heterostyly in buckwheat has been controlled by gene complex concentrate to S-locus. Homomorphic autogamous buckwheat strains were established by the interspecific hybridization. Backcrossing of this line to the common buckwheat (pin) and selecting homostylar progenies made it possible to introduce the self-compatible gene into common buckwheat. In the result, we obtained the BC₉F₂ generation, and defined the strong linkage between flower type and self-incompatibility by microscopic observation of pollen tube growth. This finding suggests that self-incompatibility character is not controlled by one gene. Moreover, we defined the strong linkage between flower type and self-incompatibility. It strongly supports the S supergene theory. Therefore, we have plan to elucidate the heterostyly self-incompatibility by using molecular genetics, proteome analysis and apply to exploitation of buckwheat improvement. In near future, the expression of heterozygous syndromes in genus Fagopyrum with single isolated heterozygous species may provide clues to early stages of polymorphic assembly and shed light on evolutionary models of heterozygous strains.

• Progress in Superconductivity and Cryogenics
• /
• v.22 no.3
• /
• pp.1-6
• /
• 2020

This paper presents the analysis results on the electrical output performance characteristics of a 746 W high temperature superconducting generator (HTSG). The HTS field winding is charged by non-contact excitation method, i.e., contactless superconducting field exciter (CSFE) which is originated by rotary flux pump based on permanent magnet. In this paper, the preliminary current charging test was carried out using a 70 A CSFE to evaluate the performance of field exciter and analyze its non-contact excitation characteristics for the full-scale HTS field winding of the 746 W HTSG. First, the various contactless current-charging tests were conducted using assembly with HTS field winding and CSFE. Then, in order to estimate the output power performance characteristics of the 746 W HTSG, finite element analysis was conducted based on field excitation information which is experimentally measured under various operating conditions. Finally, the electrical output characteristics in no-load and load models were simulated by two-dimensional transient solver in ANSYS electromagnetics 19.0 release.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.9 no.4
• /
• pp.285-291
• /
• 2004

A simulated moving bed (SMB) chromatography system is a powerful tool for preparative scale separation, which can be applied to the separation of chiral compound. We have de-signed our own lab-scale SMB chromatography using 5 HPLC pumps, 6 stainless steel columns and 4 multi-position valves, to separate a racemic mixture of ketoprofen in to its enantiomers. Our design has the characteristics of the low cost for assembly for the SMB chromatography and easy repair of the unit, which differs from the designs suggested by other investigators. It is possible for the flow path through each column to be independently changed by computer control, using 4 multi-position rotary valves and 5 HPLC solvent delivery pumps. In order to prove the operability of our SMB system, attempts were made to separate the (S)-ketoprofen enantiomer from a ketoprofen racemic mixture. The operating parameters of the SMB chromatography were calculated for ketoprofen separation from a batch chromatography experiment as well as by the triangle theory. With a feed concentration of 1 mg/mL, (S)-ketoprofen was obtained with a purity of 96% under the calculated operating conditions.

• BMB Reports
• /
• v.33 no.2
• /
• pp.138-146
• /
• 2000

In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditis elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The insitu RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C-terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.

• Biomedical Science Letters
• /
• v.22 no.4
• /
• pp.115-126
• /
• 2016

Audiometric calibration is a prerequisite for securing the reliability of audiometric test results by checking the internal consistency of the relevant instrument. The purpose of this review is to help instrument operators understand the calibration procedure of aural acoustic immittance instrument which is frequently used for objective assessment. By referring to the latest international standards and the national standards relevant to the aural acoustic immittance instrument, the following parameters will be reviewed: 1) introduction of performance characteristics, 2) detailed procedure of the calibration method. According to the newest international and national standards [IEC 60645-5 (2004), ANSI S3.39-1987 (R2012)], the aural acoustic immittance instrument basically includes six components: 1) calibration cavity, 2) acoustic immittance analysis system, 3) probe assembly/unit and signal, 4) pneumatic air-pressure pump system, 5) acoustic reflex activator system and 6) tympanogram and acoustic reflex plotting system, each of these components should meet set standards. The result of behavioral hearing tests is influenced by various complex factors including the examinee's cooperation, background noise of the examination room, measurement method, skill level of the audiologist and calibration status, but the objective hearing tests is more influenced by the calibration status of the instrument than any other factors. The audiologist should take full responsibility for the reliability of the hearing test result, so he/she should carry out the calibration check and adjustments of aural acoustic immittance instrument periodically and maintain the instrument continuously by referring to the newest standards and the manufacturer's instruction manual.

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.857_858
• /
• 2009

On account of small size and light weight, a high-speed machine is regarded as a key technology for many future applications of drive systems. In high-speed applications, permanent magnet (PM) synchronous motors have a number of merits such as high efficiency and high power density. Accordingly, they are suitable for driving the air-blower of a fuel cell electric vehicle (FCEV) where space and energy savings are critical. Particularly, a surface-mounted PM motor of them is mainly used as a high-speed machine. However, the motor has a fatal flaw owing to a retaining can to maintain the mechanical integrity of a rotor assembly. The can results in the increase of magnetic air-gap length in the surface-mounted PM motor. Thus, in this paper, an interior PM motor is designed in order to drive the air-blower of FCEV instead of the surface-mounted PM motor, and the experimental results of two models are compared to verify the capability of the interior PM motor for a high-speed machine.

• The Plant Pathology Journal
• /
• v.22 no.4
• /
• pp.386-390
• /
• 2006

Extensive research of the Potato virus X(PVX) has been performed in in vitro transcription system using the bacteriophage T7 promoter. We constructed an efficient T-DNA based binary vector, pSNU1, and modified vectors carrying PVX replicons. The suitability of the construct to transiently express PVX RNA using Agrobacterium tumefaciens was tested by analysis of infectivity in plants. The expressed PVX RNA was infectous and systemically spread in three plant species including Nicotiana benthamiana, N. tabacum cv. Xanthi-nc, and Capsicum annuum cv. Chilsungcho. The PVX full length construct, pSPVXp31, was caused severe mosaic symptoms on N. benthamiana, severe necrotic lesions on C. annuum while milder symptoms and delayed mosaic symptoms were appeared on the systemic leaves on N. tabaccum. RT-PCR analysis confirmed the presence of PVX RNAs on both inoculated and systemic leaves in all three plant species tested. Our results indicated that PVX replicons were efficiently expressed PVX RNA in at least three tested species. Further investigation win be needed to elucidate the mechanism of PVX replication, translation, movement and assembly/disassembly processes.

• IE interfaces
• /
• v.16 no.2
• /
• pp.211-221
• /
• 2003

This paper introduces a simulation study regarding the design for the installation of Color Rescheduling Storage (CRS) in an automobile factory. In the painting shop the colors of vehicles are changed frequently according to the assembly schedule. When the color of a vehicle is changed from one to another, the cleaning process of painting-gun is necessary and it generates costs. Therefore many of the automobile manufacturers equip the CRS in front of the Top Coat Booth of the painting shop. The major objective of CRS is to reduce the change over cost in the painting process by grouping vehicles having same color. In this paper the configuration of CRS and the input/output algorithms are explained. The suggested system is verified using simulation models and experiments are conducted. Finally the best alternative is suggested by sensitivity analysis and evaluation of investment feasibility.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.9
• /
• pp.1208-1213
• /
• 2012

Microcystis aeruginosa is a cyanobacterium that can form harmful algal blooms (HABs) producing toxic secondary metabolites. We provide here draft genome information of four strains of this freshwater cyanobacterium that was obtained by the Next Generation Sequencing approach to provide a better understanding of molecular mechanisms at the physiological and ecological levels. After gene assembly, genes of each strain were identified and annotated, and a genome database and G-browser of M. aeruginosa were subsequently constructed. Such genome information resources will enable us to obtain useful information for molecular ecological studies with a better understanding of modulating mechanisms of environmental factors associated with blooming.

• The Plant Pathology Journal
• /
• v.33 no.3
• /
• pp.213-228
• /
• 2017

Plasmodesmata (PDs) are specialized intercellular channels that facilitate the exchange of various molecules, including sugars, ribonucleoprotein complexes, transcription factors, and mRNA. Their diameters, estimated to be 2.5 nm in the neck region, are too small to transfer viruses or viral genomes. Tobacco mosaic virus and Potexviruses are the most extensively studied viruses. In viruses, the movement protein (MP) is responsible for the PD gating that allows the intercellular movement of viral genomes. Various host factors interact with MP to regulate complicated mechanisms related to PD gating. Virus replication and assembly occur in viral replication complex (VRC) with membrane association, especially in the endoplasmic reticulum. VRC have a highly organized structure and are highly regulated by interactions among the various host factors, proteins encoded by the viral genome, and the viral genome. Virus trafficking requires host machineries, such as the cytoskeleton and the secretory systems. MP facilitates the virus replication and movement process. Despite the current level of understanding of virus movement, there are still many unknown and complex interactions between virus replication and virus movement. While numerous studies have been conducted to understand plant viruses with regards to cell-to-cell movement and replication, there are still many knowledge gaps. To study these interactions, adequate research tools must be used such as molecular, and biochemical techniques. Without such tools, virologists will not be able to gain an accurate or detailed understanding of the virus infection process.