• Mycobiology
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• v.38 no.2
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• pp.108-112
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• 2010

To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1+ null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, $\beta$-glucosidase (Psu1), cell surface protein, glucan 1,3-$\beta$-glucosidase (Bgl2), and exo-1,3 $\beta$-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.

• 한국IT서비스학회:학술대회논문집
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• 2008.05a
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• pp.73-78
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• 2008

본 연구는 국내 이동통신기업의 글로벌 비즈니스 전략에 대한 사례연구이다. 국내 이동 통신시장의 포화에 따른 신수요 창출의 필요성과 아시아신흥시장의 급부상, 기술발전의 가속화에 따른 막대한 기술개발비용증대, 정보통신시장의 통상환경변화(FTA)에 따른 개방화와 글로벌화로 더 이상 국내 경쟁이 아닌 국경과 업계의 벽이 사라진 글로벌 경쟁이 심화되고 있다. 이러한 상황에서 국내 이동통신 업계를 대표하는 SK텔레콤의 경우 다각화된 글로벌 비즈니스 전략을 중심으로 해외시장을 지속적으로 모색하고 있다. 따라서 본 논문에서는 국내 이동통신 기업의 글로벌 비즈니스 전략을 SK텔레콤의 사례를 통해서 살펴보기로 한다.

• Analytical Science and Technology
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• v.18 no.6
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• pp.460-468
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• 2005

The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. $60{\AA}$ thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and $7.5{\times}10^{-12}mol/cm^2$, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1856-1857
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• 2007

본 논문에서는 증강현실을 이용한 조립교육 트레이닝 방법을 제안하였다. 제안된 조립교육 방법이 기존의 문자나 그림을 이용한 조립교육 방법과 비교하여 사용자 (교육자)에게 얼마나 효과적인지를 실제 조립교육 실험을 통하여 검토되었다. 일반 PC 조립교육에 대한 비교 결과, 문자/이미지에 기초한 2D 형식의 기존 방법 보다 제안된 본 연구 방법이 조립시간을 단축시킴으로써, 결과적으로 조립교육 효과에 긍정적임을 확인할 수 있었다.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.323-333
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• 2015

As sessile organisms, plants are exposed to persistently changing stresses and have to be able to interpret and respond to them. The stresses, drought, salinity, chemicals, cold and hot temperatures, and various pathogen attacks have interconnected effects on plants, resulting in the disruption of protein homeostasis. Maintenance of proteins in their functional native conformations and preventing aggregation of non-native proteins are important for cell survival under stress. Heat shock proteins (HSPs) functioning as molecular chaperones are the key components responsible for protein folding, assembly, translocation, and degradation under stress conditions and in many normal cellular processes. Plants respond to pathogen invasion using two different innate immune responses mediated by pattern recognition receptors (PRRs) or resistance (R) proteins. HSPs play an indispensable role as molecular chaperones in the quality control of plasma membrane-resident PRRs and intracellular R proteins against potential invaders. Here, we specifically discuss the functional involvement of cytosolic and endoplasmic reticulum (ER) HSPs/chaperones in plant immunity to obtain an integrated understanding of the immune responses in plant cells.

• Korean Journal of Materials Research
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• v.9 no.6
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• pp.595-598
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• 1999

Ultra-thin films of amphiphilic Squarylium dye were prepared on the hydrophilic glass substrate by Langumuir-Blodgett(LB) technique. From the measurement of the surface pressure-area($\pi$-A) isotherm at air-water interface, it was found that amphiphilic Squarylium dye can form the stable monolayers. Using the LB technique, the Z-type monolayer assembly can be obtained. The amphiphilic Squarylium dye LB films exhibit λ\ulcorner at 684nm. The absorption is significantly red-shifted from solution of amphiphilic Squarylium dye(637nm in chloroform), suggesting that the Squarylim chromophores form J-aggregate in the LB film.

• Mycobiology
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• v.46 no.4
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• pp.349-360
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• 2018

Whole-genome sequencing of Flammulina ononidis, a wood-rotting basidiomycete, was performed to identify genes associated with carbohydrate-active enzymes (CAZymes). A total of 12,586 gene structures with an average length of 2009 bp were predicted by the AUGUSTUS tool from a total 35,524,258 bp length of de novo genome assembly (49.76% GC). Orthologous analysis with other fungal species revealed that 7051 groups contained at least one F. ononidis gene. In addition, 11,252 (89.5%) of 12,586 genes for F. ononidis proteins had orthologs among the Dikarya, and F. ononidis contained 8 species-specific genes, of which 5 genes were paralogous. CAZyme prediction revealed 524 CAZyme genes, including 228 for glycoside hydrolases, 21 for polysaccharide lyases, 87 for glycosyltransferases, 61 for carbohydrate esterases, 87 with auxiliary activities, and 40 for carbohydrate-binding modules in the F. ononidis genome. This genome information including CAZyme repertoire will be useful to understand lignocellulolytic machinery of this white rot fungus F. ononidis.

• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.191-193
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• 2021

Lactococcus lactis is a fermentative lactic acid bacterium that is used extensively in food fermentations. The L. lactis strain K_LL005 was isolated from the grasshopper (Oxya chinensis sinuosa) gut in Korea. In this study, we reported the complete genome sequence of Lactococcus lactis K_LL005. The final complete genome assembly consist of one circular chromosome (2,375,093 bp) with an overall guanine + cytosine (G + C) content of 35.0%. Annotation results revealed 2,281 protein-coding sequences (CDSs), 19 rRNAs, and 68 tRNA genes. Lactococcus lactis K_LL005 has a gene encoding xylose metabolism such as xylR, xylA, and xylB (xylRAB).

• Molecules and Cells
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• v.45 no.1
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• pp.6-15
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• 2022

Phase separation is a thermodynamic process leading to the formation of compositionally distinct phases. For the past few years, numerous works have shown that biomolecular phase separation serves as biogenesis mechanisms of diverse intracellular condensates, and aberrant phase transitions are associated with disease states such as neurodegenerative diseases and cancers. Condensates exhibit rich phase behaviors including multiphase internal structuring, noise buffering, and compositional tunability. Recent studies have begun to uncover how a network of intermolecular interactions can give rise to various biophysical features of condensates. Here, we review phase behaviors of biomolecules, particularly with regard to regular solution models of binary and ternary mixtures. We discuss how these theoretical frameworks explain many aspects of the assembly, composition, and miscibility of diverse biomolecular phases, and highlight how a model-based approach can help elucidate the detailed thermodynamic principle for multicomponent intracellular phase separation.

• Nuclear Engineering and Technology
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• v.54 no.9
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• pp.3260-3271
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• 2022

Experience shows that the solution to waste management in any national programme is lengthy and burdened with uncertainties. There are several uncertainties that contribute to the costs associated with spent fuel management. In this work, we have analysed the impact of the current nuclear data on the isotopic composition of the spent fuel and consequently their influence on the main spent fuel observables such as decay heat, activity, neutron multiplication factor, and neutron and photon source terms. Nuclear libraries based on the most general nuclear data ENDF/B-VII.0, ENDF/B-VII.1, ENDF/B-VIII.0 and JEFF-3.3 are considered. A typical NPP Krško fuel assembly is analysed using the Monte Carlo code Serpent 2. The analysis considers burnup of up to 60 GWd/tU and cooling times of up to 100 years. The comparison of results showed significant differences, which should be taken into account when selecting the library and evaluating the uncertainty in determining the characteristics of the spent fuel.