• Molecules and Cells
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• v.39 no.11
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• pp.814-820
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• 2016

FtsZ, a tubulin homologue, is an essential protein of the Z-ring assembly in bacterial cell division. It consists of two domains, the N-terminal and C-terminal core domains, and has a conserved C-terminal tail region. Lateral interactions between FtsZ protofilaments and several Z-ring associated proteins (Zaps) are necessary for modulating Z-ring formation. ZapD, one of the positive regulators of Z-ring assembly, directly binds to the C-terminal tail of FtsZ and promotes stable Z-ring formation during cytokinesis. To gain structural and functional insights into how ZapD interacts with the C-terminal tail of FtsZ, we solved two crystal structures of ZapD proteins from Salmonella typhimurium (StZapD) and Escherichia coli (EcZapD) at a 2.6 and $3.1{\AA}$ resolution, respectively. Several conserved residues are clustered on the concave sides of the StZapD and EcZapD dimers, the suggested FtsZ binding site. Modeled structures of EcZapD-EcFtsZ and subsequent binding studies using bio-layer interferometry also identified the EcFtsZ binding site on EcZapD. The structural insights and the results of bio-layer interferometry assays suggest that the two FtsZ binding sites of ZapD dimer might be responsible for the binding of ZapD dimer to two protofilaments to hold them together.

• Journal of Plant Biotechnology
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• v.36 no.3
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• pp.268-274
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• 2009

Porphyromonas gingivalis, the gram-negative anaerobic oral bacterium, initiates periodontal disease by binding to saliva-coated oral surface. The cholera toxin B subunit (CTB) genetically linked to FimA1 (1-200 aa) or FimA2 (201-337 aa) of the P. gingivalis fimbrial antigen were introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation method. The integration of CTB-FimA1 or CTB-FimA2 fusion genes were confirmed in the chromosome of transformed leaves by genomic DNA PCR amplification method. Synthesis and assembly of the CTB-FimA fusion proteins into oligomeric structures with pentamer size was detected in transformed tuber extracts by immunoblot analysis. The binding activities of CTB-FimA fusion proteins to intestinal epithelial cell membrane receptors were confirmed by GM1-ganglioside enzyme-linked immunosorbent assay (GM1-ELISA). The ELISA showed that the expression levels of the CTB-FimA1 or CTB-FimA2 fusion proteins were 0.0019, 0.002% of the total soluble protein in transgenic tuber tissues, respectively The synthesis of CTB-FimA monomers and their assembly into biologically active oligomers in transformed potato tuber tissues demonstrates the feasibility of using edible plants for the production of enterocyte targeted fimbrial antigens that could elicit mucosal immune responses.

• Journal of Sensor Science and Technology
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• v.30 no.4
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• pp.229-235
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• 2021

This study presents a simple approach for the assembly of a free-standing conductive electronic nanofilm of single-walled carbon nanotubes (SWNTs) suitable for enzymatic electrochemical biosensors. A large-scale SWNT electronic film was successfully produced by the dialysis of p-Terphenyl-4,4''-dithiol (TPDT)-treated SWNTs. Furthermore, Horseradish peroxidase (HRP) was immobilized on the TPDT-SWNT electronic film, and the enzymatic detection of hydrogen peroxide (H₂O₂) was demonstrated without mediators. The detection of H₂O₂ in the negative potential range (-0.4 V vs. Ag/AgCl) was achieved by direct electron transfer of heme-based enzymes that were immobilized on the TPDT-SWNT electronic film. The SWNT-based biosensor exhibited a wide detection range of H₂O₂ from 10 µM to 10 mM. The HRP-doped SWNT electronic film achieved a high sensitivity of 342 ㎛A/mM·cm² and excellent selectivity against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, and acetaminophen.

• Korean Journal of Construction Engineering and Management
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• v.19 no.1
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• pp.43-53
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• 2018

Modular construction can improve construction quality and accuracy through manufacturing process, and it allow massive production and cost savings by repeatedly producing the same unit. In particular, it is possible to reduce the time because the on-site work and the manufacturing process can be carried out at simultaneously. However, according to the modular construction project survey report, there is no significant difference regarding the average construction period between modular construction and conventional construction. This is due to schedule delay problems that occur during construction phase. Therefore, it is necessary to select alternatives to prevent schedule delay during on-site construction progressing. Especially, in case of large-scale modular construction project, on-site module assembly and manufacturing process are performed concurrently. Hence, identification of alternatives should be done at the co-occurrence by taking both manufacturing and on-site work process in to account. In this research, the management factors of large-scale modular construction project were identified through the IDEF0 modeling, and the quantity management model for manufacturing and assembly is developed. This will reduce the schedule delay problem that occurs in the progression on-site work of a large scale modular construction.

• The Korean Journal of Archival Studies
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• no.44
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• pp.5-49
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• 2015

Although a study on documentation strategy has been steadily releasing recently in the archival study field, it is rare that the cases or studies applying the institutional functional analysis methodology that Helen Samuels had suggested. The institutional functional analysis is a methodology to provide a comprehensive understanding of the record by defining the essential features of the institution at a macro level. The documentation area derived from the institutional functional analysis functions as a priori framework for an archivist to select and keep the records of the institution. In other words, by analyzing the function of the institution through the institutional functional analysis methodology, it makes possible to identify the functions not covered under the current records retention schedule at a macro level ensuring accountability of the organization's business activities. The documentation project of the United States is a prime example to comply with this background. In this study, by analyzing the case of the United States Congress, it is proposed the derived implications for applying the institutional functional analysis to the National Assembly of the Republic of Korea, where is the similar national institution to the U.S. Congress. For this purpose, firstly, the theoretical debate on the institutional functional analysis as the previous studies. Secondly, the documentation project of the United States Congress has been case analyzed by each function according to the institutional functional analysis. Thirdly, on the basis of the case study results, the implications are derived and it is suggested that the documentation areas applicable to the National Assembly of the Republic of Korea.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.22 no.4
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• pp.735-739
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• 2013

The error motion of a machine tool spindle directly affects the surface errors of machined parts. Spindle motion errors such as three translational motions and two rotational motions are undesirable. These are usually due to the imperfectness of bearings, stiffness of spindle, assembly errors, and external force or unbalance of rotors. The error motions of the spindle need to be reduced for achieving the desired performance. Therefore, the level of error motion needs to be estimated during the design and assembly process of the spindle. In this study, an estimation method for five degree-of-freedom (5 DOF) error motions for a spindle with an angular contact ball bearing is suggested. To estimate the error motions of the spindle, the waviness of the inner-race of bearings and an external force model were used as input data. The estimation model considers the geometric relationship and force equilibrium of the five DOFs. To calculate the error motions of the spindle, not only the imperfections of the shaft and bearings but also driving elements such as belt pulley and direct driving motor systems are considered.

• Journal of the Korean housing association
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• v.25 no.2
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• pp.11-18
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• 2014

This study looked at designing, building and operating temporary camp, the first structures that South Korea built in the Antarctica. While there may be differences in accordance with the topography, ground surfaces in the Antarctica are covered broken stones, glaciers and snow. Hence, such topographical characteristics should be taken into account when conducting any construction work. To ensure successful assembly construction in the Antarctica using modules, prior trial assembly work should be done in Korea to identify any possible trouble in the actual construction process. Assuming that the workers will have to spend at least one winter in the temporary camp, the work will be more severely affected by adverse weather conditions and snow drift, resulting in the need to clear snow. This can be by designing roofs with curved surfaces. Also, quantitative effects will need to be verified through simulation and actual measurement. It will also be necessary to assess the camp's thermal environment and examine its air-conditioning methods. To identify the temporary camp's thermal system, the temperatures and humidities were measured, and the heating system was designed not to offer automatic control or desired value selection functions.

• Proceedings of the Korean Geotechical Society Conference
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• 2007.09a
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• pp.611-622
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• 2007

In the design and construction of the coastal/offshore structures, it is very important to evaluate the geotechnical characteristics of marine soils, which support the structures. Although the offshore site investigation is much more difficult than onshore, safe and precise jobs have not been accomplished in Korea because of the insufficiency of the test equipments especially for the site with deep water depth. The main objective of this study is to develop a new type of marine cone penetration testing(CPT) system, which can be utilized to even deep sea and high depth of soil layer. The system is one of seabed types and employs the conventional cone, which shows more reliable results than miniature cone. The most important parts of the marine CPT including continuous rod system, cone penetration system with wheel drive, automatic cone rod assembly/dissembly system etc., were designed and manufactured. Some tests to verify the developed marine CPT system were performed at both onshore and offshore sites as well as mechanical test in laboratory. The test results show the consistent and promising performance of the new equipment, and thereafter the system would be applicable to various sites with practical/economical advantages.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.66.3-66.3
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• 2017

Korea Astronomy and Space Science Institute (KASI) is developing a coronagraph in collaboration with National Aeronautics and Space Administration (NASA) which will be installed on the International Space Station (ISS). The coronagraph can measure speed and temperature by using four filters approximately 400 nm and polarization filter in three different angles, differently with older coronagraphs. For the successful mission, it has development and experiment progress in three phases; total solar eclipse experiment in 2017, balloon experiment in 2019, and the ISS installation in 2021. As a first experiment, we developed a coronagraph without an occulter named with Diagnostic Coronagraph Experiment (DICE) for experiment for filter system and imaging sensor. We designed optics with a field of view from 2.5 to 15 solar radii. It has four filters approximately 400 nm and polarizer to measure speed and velocity of the solar corona. For the selection of filter or polarization angle, it has two mechanism parts; filter wheel assembly and a polarizer wheel assembly. Especially we used Core Flight System (CFS) platform which was developed by NASA, when we develop the coronagraph operation software. It provides us stability, reusability, and compatibility.

• Journal of the Korean Society of Propulsion Engineers
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• v.20 no.6
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• pp.91-100
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• 2016

Efficiency characteristics of centrifugal turbopumps for a liquid rocket engine were investigated. Predicting the performance of pumps for a turbopump assembly test, the variation on pump efficiency by working fluids was analyzed from pump component tests. Water and liquid nitrogen (LN2) were used for the component test, kerosene (Jet A-1) and liquid oxygen (LOX) were adapted for the turbopump assembly (TPU) test as working fluids. Overall performance of the pumps was investigated covering design/off-design operating points and the pump efficiency on the environment of real media (LOX/kerosene) could be modified from the pump component tests.