• Bioinformatics and Biosystems
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• v.1 no.1
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• pp.61-66
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• 2006

We propose a new fragment assembly program MLP (mate-based layout with PHRAP). MLP consists of PHRAP, repeat masking, and a new layout algorithm that uses the mate pair information. Our experimental results show that by using MLP instead of PHRAP, we can significantly reduce the difference between the assembled sequence and the original genome sequence.

• Molecules and Cells
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• v.37 no.2
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• pp.126-132
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• 2014

As a tumor suppressor homologue during mitosis, Chk2 is involved in replication checkpoints, DNA repair, and cell cycle arrest, although its functions during mouse oocyte meiosis and early embryo development remain uncertain. We investigated the functions of Chk2 during mouse oocyte maturation and early embryo development. Chk2 exhibited a dynamic localization pattern; Chk2 expression was restricted to germinal vesicles at the germinal vesicle (GV) stage, was associated with centromeres at pro-metaphase I (Pro-MI), and localized to spindle poles at metaphase I (MI). Disrupting Chk2 activity resulted in cell cycle progression defects. First, inhibitor-treated oocytes were arrested at the GV stage and failed to undergo germinal vesicle breakdown (GVBD); this could be rescued after Chk2 inhibition release. Second, Chk2 inhibition after oocyte GVBD caused MI arrest. Third, the first cleavage of early embryo development was disrupted by Chk2 inhibition. Additionally, in inhibitor-treated oocytes, checkpoint protein Bub3 expression was consistently localized at centromeres at the MI stage, which indicated that the spindle assembly checkpoint (SAC) was activated. Moreover, disrupting Chk2 activity in oocytes caused severe chromosome misalignments and spindle disruption. In inhibitor-treated oocytes, centrosome protein ${\gamma}$-tubulin and Polo-like kinase 1 (Plk1) were dissociated from spindle poles. These results indicated that Chk2 regulated cell cycle progression and spindle assembly during mouse oocyte maturation and early embryo development.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.20 no.4
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• pp.168-172
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• 2010

Superhydrophilic thin film consisted of positively charged poly (allylamine hydrochloride) (PAH) and negatively charged poly (acrylic acid) (PAA) was fabricated by a layer-by-layer (LBL) self-assembly. Glutaraldehyde (GA) was used in order to increase an adhesion hardness by amine-aldehyde reaction. The surface morphology, thickness, transmittance, water contact angle and adhesion hardness of PAH/PAA thin film with or without GA were measured. The adhesion hardness of PAH/PAA thin film with GA deposition increased over 2 times although the film thickness of PAH/GA/PAA decreased than that of PAH/PAA thin film. The increase of adhesion hardness by amine-aldehyde reaction between PAH and GA was measured by fourier transform infrared (FT-IR) spectroscopy. Fabricated PAH/PAA and PAH/GA/PAA thin films showed water contact angel under $5^{\circ}$ and high transmittance over 91.3% at 550 nm.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.05a
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• pp.269-273
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• 2010

7.7 DDoS incident due to recent events and the emerging importance of privacy and Privacy laws are being discussed in the National Assembly. In this study, Legislative Assembly Secretariat support organization focused on using the system, such as the Internet network and the administrative, technical and physical security elements on the status of confidentiality, availability, integrity and security criteria to identify and follow and We are analyzing. In addition, the Internet, including network and use the system primarily for use, Legislative support agency, The National Assembly Secretariat staff awareness about information security and privacy on the survey for compliance with codes of conduct and We are analyzing. Through this analysis of legislative support agencies' security status, and social responsibility as an institution will wish to encourage the role.

• Journal of Applied Reliability
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• v.14 no.1
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• pp.45-51
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• 2014

Thermal shock tests at two stress levels were performed to see the life (cycles) of LPF ASSY (low pass filter assembly) at normal stress level. In this case Coffin-Manson relationship is generally used to describe the relationship between the temperature difference and the life, together with the Weibull distribution describing the life at each stress level. So for given data Coffin-Manson is fitted to predict the life at normal stress level. However, different types of models are appropriate for this type of test. Hence, a more appropriate model such as General log-linear model which can also incorporate the duration at the highest and lowest temperatures and acceleration time will be introduced.

• Journal of the Korea Society of Computer and Information
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• v.21 no.2
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• pp.97-103
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• 2016

This paper deals with the cycle time minimization problem that determines the productivity in printed circuit board (PCB) with n components using the m placement machines. This is known as production cycle time determination problem (PCTDP). The polynomial time algorithm to be obtain the optimal solution has been unknown yet, therefore this hard problem classified by NP-complete. This paper gets the initial assignment result with the machine has minimum unit placement time per each component firstly. Then, the balancing process with reallocation from overhead machine to underhead machine. Finally, we perform the swap optimization and get the optimal solution of cycle time $T^*$ within O(mn) computational complexity. For experimental data, the proposed algorithm can be obtain the same result as integer programming+branch-and-bound (IP+B&B) and B&B.

• Journal of the Semiconductor & Display Technology
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• v.16 no.3
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• pp.59-64
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• 2017

As the demand on displays increases, new thin-film transistors such as metal oxide transistor are continuously being invented. When designing a circuit consisting of such new transistors, a new transistor model based on proper charge transport mechanisms is needed for each of them. In this paper, a modeling framework which enables to choose charge transport mechanisms that are limited to certain operation regions and assemble them into a transistor model instead of making an integrated transistor model dedicated to each transistor. The framework consists of a graphic user interface to choose charge transport models and a current calculation part, which is also implemented in AIM-SPICE for circuit simulation.

• Applied Microscopy
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• v.52
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• pp.11.1-11.9
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• 2022

Zoonotic poxvirus infections pose significant threat to human health as we have witnessed recent spread of monkeypox. Therefore, insights into molecular mechanism behind poxvirus replication cycle are needed for the development of efficient antiviral strategies. Virion assembly is one of the key steps that determine the fate of replicating poxviruses. However, in-depth understanding of poxvirus assembly is challenging due to the complex nature of multi-step morphogenesis and heterogeneous virion structures. Despite these challenges, decades of research have revealed virion morphologies at various maturation stages, critical protein components and interactions with host cell compartments. Transmission electron microscopy has been employed as an indispensable tool for the examination of virion morphology, and more recently for the structure determination of protein complexes. In this review, we describe some of the major findings in poxvirus morphogenesis and the contributions of continuously advancing electron microscopy techniques.

• Publications of The Korean Astronomical Society
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• v.30 no.1
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• pp.1-9
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• 2015

The National Foundation Day of Korea (개천절, 開天節) is currently celebrated on October 3 in Gregorian calendar. We review the history of dating the National Foundation Day of Korea and make a suggestion that it be celebrated on October 3 in the lunar calendar. We present numerous historical records on heaven-worship rites supporting the date October 3 in the lunar calendar. It is pointed out that October 3 in the solar calendar has been adopted in 1949 by the National Assembly with the thought that the lunar calendar is inferior and behind the times. The thought originates from misunderstanding on the value of the lunar calendar and from the ignorance of importance of history and tradition. Since there are now many national holidays that follow the lunar calendar, the logic of the National Assembly in 1949 also makes no sense. We emphasize that the lunar calendar should be followed for the National Foundation Day of Korea for its historical and symbolic characteristics restoration. We also investigate the year of the foundation of the first country of Korea, Dangun Joseon. It is found that even though the majority of the literature before late 15th century recorded the beginning year of Dangun Joseon dynasty to be equal to that of Liao Dynasty (堯), it was accidentally changed to the 25th year of Liao Dynasty in 1484 through a misinterpretation of the previous records. We claim that the beginning year of Dangun Joseon should be set to that of Liao Dynasty as recorded in the original literature in the earlier days. According to the two main opinions accepted by Korea, the beginning year of Liao Dynasty was 2357 B.C. or 2333 B.C., which correspond to the year of Gap-Jin (the 41st year of the sexagenary cycle) or Mu-Jin (the 4th year of the sexagenary cycle), respectively.

• Korean Chemical Engineering Research
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• v.50 no.5
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• pp.778-782
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• 2012

Until a recent day, degradation of PEMFC MEA (membrane and electrode assembly) has been studied, separated with membrane degradation and electrode degradation, respectively. But membrane and electrode were degraded coincidentally at real PEMFC operation condition. Therefore in this work, AST (Accelerated Stress Test) of MEA degradation was done at the condition that membrane and electrode were degraded simultaneously. There was interaction between membrane degradation and electrode degradation. Membrane degradation reduced the decrease range of catalyst active area by electrode degradation. Electrode degradation reduces increase range of the hydrogen crossover current and FER (Fluoride Emission Rate) by membrane degradation.