• Title/Summary/Keyword: Koji

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The Changes in Organic Acids and Fatty Acids in Kochujang Prepared with Different Mashing Methods (담금방법을 달리한 고추장의 유기산 및 지방산의 변화)

  • Chun, Myung-Sook;Lee, Taik-Soo;Noh, Bong-Soo
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.25-29
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    • 1995
  • Organic acids and fatty acids of four different Kochujang prepared with different mashing methods were determined by HPLC and GC. Citrate, malate, lactate, succinate, formate, oxalate and acetate were identified. Citrate was found as a major organic acid ($450{\sim}565$ mg%) followed in decreasing order by lactate and malate. Total organic acid content of Meju Kochujang was the highest at the initial fermentation time, while that of koji Kochujang was higher than any other one after 90 days of fermentation. There was a little change of total acid among the treatments. Oleic acid was a major fatty acid ($68.59{\sim}75.38%$) during the fermentation of Kochujang. There was no significant change of fatty acids during the fermentation and among the treatments.

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Continuous rapid Production of Soy Sauce by Coimmobilized Mixed Culture system of Zygosaccharomyces rouxii and Candida versatilis using Air Bubble Column Reactor (Zygosaccharomyces rouxii와 Candida versatilis의 동시 고정화에 의한 Air Bubble Column Reactor에서 간장의 연속적 속성 생산)

  • 류병호
    • KSBB Journal
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    • v.18 no.6
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    • pp.455-460
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    • 2003
  • This study was designed to find out the rapid fermentation of soy sauce from koji hydrolyzates using air bubble column reactor packed with coimmobilized mixed culture system. Continuous ripid production was performed by coimmobilized Z. rouxiii BH-90 and C. versatilis BH-91. Coimmobilized cells of Zygosaccharomyces rouxii BH-90 and Candida versatilis BH-91 mixture cells in the column reactor produced 2.8% ethyl alcohol and 18mg/L 4-ethylguaiacol over 96 hours under the optimal conditions. Coimmobilized cells produced 2.30∼2.4% ethyl alcohol during 30 days, and decreased gradually from 40 days to 70 days. Also coimmobilized cells produced 4-ethylguaiacol at the constant rate of 16∼18mg/L and decreased gradually after 40 days. Final product of soy sauce contained 2.4% ethyl alcohol and 18mg/L 4-ethylguaiacol. However, amino acid compositions of soy sauce were consisted of predominantly glutamic acid, leucin, arginine, aspartic acid, Iysine and valine, which were more than 50% of total amino acid.

Histological Changes of Doenjang during the Fermentation with Different Strains (균주를 달리한 된장의 발효기간에 따른 대두의 조직학적 변화에 관한 연구)

  • Park, Jung-Suk
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.477-481
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    • 1992
  • As a series of fundamental research projects to produce doenjang (Korean fermented soy paste) of better quality, two kinds of doenjang were manufactured from a traditional meju (Korean soy bean koji) and the mixed with Aspergillus oryzae and Bacillus natto, and histological changes in the cell structures of soy bean of the two were reported doenjang samples were observed and compared during the entire period of fermentation processes. Cell walls of the soy bean were ruptured by pressure and heat during the pressure cooking process and some of them were observed to have the ghost-like shapes. Remarkable differences in the plasmolysis of the cytoplasms were observed between the seed coat and the inner part of soy bean. Small vacuoles resulting from the fusion of the glycoprotein globules by protease and from the hydrolysis of the starch granules by amylase were also observed. Penetration of microorganisms was transferred from the seed coat to the inside of soy bean as the fermentation proceeded. Slimy substances were observed on the seed coat and the parenchyma cells of soy bean fermented with the mixed with Aspergillus oryzae and Bacillus natto. Cell walls of soy bean became difficult to stain and they showed unusual, polygonal shapes as the fermentation proceeded. Samples fermented with the mixed with Aspergillus oryzae and Bacillus natto showed more remarkable tendencies than traditional meju.

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Microstructure and Mechanical Properties of Hardmaterials

  • Hayashi, Koji
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 1994.04c
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    • pp.6-6
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    • 1994
  • Har dmaterials such as cemented carbides with or without coated layer, cermets, ceramics and diamond or c-BN high pressure sintered compact are used for cutting tools, wear -resistant parts, rock drilling bits and/or high pressure vessels. These hardmaterials contain not only hard phase, but also second consituent as the element for forming ductile phase and/or sintering aid, and the mechanical properties of each material depend on (1) the amount of the second constituent as well as (2) the grain size of the hard phase. The hardness of each material mainly depends on these two factors. The fracture strength, however, largely depends on other microstructur a1 factors as well as the above two factors. For all hardmaterials, the fracture strength is consider ably affected by (3) the size of microstructur a1 defect which acts as the fracture source. In cemented carbides, the following factors which are generated mainly due to the addition of the second constituent are also important; (4) the variation of the carbon content in the normal phase region free from V-phase and graphite phase, (5) the precipitation of $Co_3$ during heating at about $800^{\circ}C$,(6) the domain size of binder phase, and (7) the formation of ${\beta}$-free layer or Co-rich layer near the surface of sintered compacts. For cemented carbides coated with thin hard substance, the important factors are as follows; (8) the kind of coated substance, (9) the formation of ${\eta}$-phase layer at the interface between coated layer and substrate, (10) the type of residual stress (tension or compression) in the coated layer which depends on the kind of coating method (CVD or PVD), and (11) the properties of the substrate, and (12) the combination, coherency and periodicity of multi-layers. In the lecture, the details of these factors and their effect on the strength will be explained.

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A Polarimetric Study of Long-Period Comet C/2013 US10 (Catalina) and Estimation of Its Gas Contamination in Optical and Near-Infrared Wavelengths

  • Kwon, Yuna Grace;Ishiguro, Masateru;Kuroda, Daisuke;Hanayama, Hidekazu;Kawabata, Koji S.;Akitaya, Hiroshi;Itoh, Ryosuke;Nakaoka, Tatsuya
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.48.1-48.1
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    • 2016
  • Polarimetric study of light scattering from cometary dust particles can provide us opportunity to decipher their characteristics, such as sizes, structures, compositions of dust grains, etc. Herein, we present the results of our polarimetric study of long-period comet, C/2013 US10 (Catalina), in optical and near-infrared wavelengths which appeared at large phase angle (52.7 degrees) around the mid-December, 2015. We performed polarimetric and spectroscopic observations with HONIR, attached to the 1.5-m telescope at Higashi-Hiroshima Observatory, on UT 2015 December 17-18 and also obtained optical imaging data sets by the Ishigakijima Astronomical Observatory (IAO) and Okayama Astrophysical Observatory (OAO) taken between 2014-2015. By measuring the intensities of gas emission lines with respect to dust continuum and considering transmittance of each filter, we estimated that the percentages of gas contamination are approximately 10 percents in $R_C$-band and 3 percents in $I_C$-band. With these results, we derive the degree of linear polarization scattered solely from dust components in the coma. At this presentation, we will compare the phase-angle dependence of the degree of linear polarization with those of previous archive data in a wide coverage of wavelengths from $R_C$-band to $K_S$-band. Finally, we are supposed to discuss the spatial variations in polarization within the coma.

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The Quality of Yakju be brewed from many kind of Nuruk (누룩에 따른 약주의 품질 평가)

  • 이미경;이성우;배상면
    • Journal of the East Asian Society of Dietary Life
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    • v.1 no.1
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    • pp.99-111
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    • 1991
  • In each nuruk using today, effect of pH on glucoamylase activity and viable cell count of yeast and bacteria was measured. Common components during fermentation, alcohol, acetaldehyde and acetone, amino acid composition, and total sugars and mineral content were determined in yakju(korean wine) brewed from different ingredients and by different methods. Results are summarized as follows ; 1. The lower the pH, the lower the glucoamylase activity in JK, BK, JK-S BK-S and JN. But the higher the glucoamylase activity ratio in Koji and KN. 2. Yeast and bacteria cell count could not determined in nuruk inoculated of seed. In JK, BK and JN, yeast cell count was 50${\times}$104∼80${\times}$104, bacteria cell count was 5${\times}$106∼24${\times}$106. 3. In yakju during fermentation, pH was higher in RU, total acidity content was higher in ST-N, ST-K, RU and ST-RUPO and alcohol content was lower in RUPO and ST-RUPO. 4. Ethanol and acetaldehyde content were highest in dukyunju. Trace amount of acetone was determined only in ST-K, RUPO and ST-RUPO . n-Propyl alcohol content was higher in ST-K, ST-RUPO and ST-N, iso-butyl alcohol content was higher in L-RUPO, Dukyunju and Songyupju and iso-amyl alcohol content was higher in Songyupju, RU, L-RUPO and Dukyunju. 5. In amino acids composition of each yakju, Pro, Ala and Val content was higher than other amino acids. Total amino acids content was the highest in Dukyunju and second highest in ST-N, NH3 was higher in ST-N, Dukyunju, RUPO than other samples. 6. Total sugars content was the highest in ST-N and second highest in RU. 7. P, K and Mg content were higher in Dukyunju and ST-N than in other samples. In Dukyunju, Ca and P ratio was 0.075 because of low Ca content and high P content.

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Degradation of Raw Starch Granules by α-Amylase Purified from Culture of Aspergillus awamori KT-11

  • Matsubara, Takayoshi;Ammar, Youssef Ben;Anindyawati, Trisanti;Yamamoto, Satoru;Ito, Kazuo;Iizuka, Masaru;Minamiura, Noshi
    • BMB Reports
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    • v.37 no.4
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    • pp.422-428
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    • 2004
  • Raw-starch-digesting $\alpha$-amylase (Amyl III) was purified to an electrophoretically pure state from the extract of a koji culture of Aspergillus awamori KT-11 using wheat bran in the medium. The purified Amyl III digested not only soluble starch but also raw corn starch. The major products from the raw starch using Amyl III were maltotriose and maltose, although a small amount of glucose was produced. Amyl III acted on all raw starch granules that it has been tested on. However, it was considered that the action mode of the Amyl III on starch granules was different from that of glucoamylase judging from the observation of granules under a scanning electron microscope before and after enzyme reaction, and also from the reaction products. Glucoamylase (GA I) was also isolated and it was purified to an electrophoretically pure state from the extract. It was found that the electron micrographic features of the granules after treatment with the enzymes were quite different. A synergistic effect of Amyl III and GA I was observed for the digestion of raw starch granules.

Fabrication Methods of Porous Ceramics and Their Applications in Advanced Engineering - Large Flat Precision Plate for Flat Display Industries

  • Matsumaru, Koji;Ishizaki, Kozo
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.3.1-3.1
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    • 2009
  • Normal sintering process of producing porous ceramics is not to sinter perfectly, i.e., stop sintering in middle-process. Our porous ceramic materials are a product of complete sintering. For example if one want to make a porous carborundum, raw carborundum powder is sintered at either lower temperatures than normal sintering temperature or shorter sintering periods than normal sintering time to obtain incompletely sintered materials, i.e., porous carborundum. This implies normally sintered porous ceramic materials can mot be used in high vacuum conditions due to dust coming out from uncompleted sintering. We could produce completely sintered porous ceramic materials. For example, we can produce porous carborundum material by using carborundum particles bonded by glassy material. The properties of this material are similar to carborundum. We could make quasi-zero thermal expansion porous material by using carborundum and particles of negative thermal expansion materials bonded by the glassy material. We apply to sinter them also by microwave to sinter quickly. We also use HIP process to introduce closed pores. We could sinter them in large size to produce $2.5m{\times}2.5m$ ceramic plate to use as a precision plate for flat display industries. This flat ceramic plate is the world largest artificial ceramic plate. Precision plates are basic importance to any advanced electronic industries. The produced precision plate has lower density, lower thermal expansivity, higher or similar damping properties added extra properties such as vacuum vise, air sliding capacity. These plates are highly recommended to use in flat display industries. We could produce also cylindrical porous ceramics materials, which can applied to precision roller for polymer film precision motion for also electronic industries.

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Studies on the Formation of Organic Acid and Saccharifing Amylase in Koji Culture of Aspergillus usamii shirousamii $U_2$ -Part II. Effect of antimicrobial agents- (Aspergillus usamii shirousamii $U_2$균(菌)의 국식배양(麴式培養)에 의(依)한 유기산(有機酸) 및 당화효소(糖化酵素) 생성(生成)에 관(關)한 연구(硏究) -제2보 항균제(抗菌劑)의 영향에 대(對)하여-)

  • Youn, Bok-Hyun;Lee, Suk-Kun;Youn, Han-Kyo
    • Korean Journal of Food Science and Technology
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    • v.7 no.4
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    • pp.238-242
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    • 1975
  • The effects of various antimicrobial agents on the production of organic acid and saccharifying amylase by Aspergillus usamii shirousamii $U_2$ were studied and the results are as follows. 1) The production of organic acid and saccharifying amylase was stimulated by using optimum amounts of nitrofurazone and $AF_2$ among several antimicrobial agents. 2) Wheat flour medium containing 50% volumes of 10 ppm $AF_2$ solution gave the maximum yield of organic acid. 3) Wheat bran medium containing 100% volumes of $100{\sim}500ppm$ nitrofurazone solution gave the maximum yield of saccharifying amylase.

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Application of DV-X$\alpha$ Method to ${\gamma}$-2CaO.SiO$_2$

  • Yamaguchi, Norio;Fujimori, Hirotaka;Ioku, Koji;Goto, Seishi;Nakayasu, Tetsuo
    • The Korean Journal of Ceramics
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    • v.6 no.4
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    • pp.339-342
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    • 2000
  • In the present study, we attempted to apply DV-X$\alpha$ method to expressing the reactivity of materials. The expression of reactivity was discussed by comparison between ${\gamma}$-C$_2$G having hydraulic activity and ${\gamma}$-C$_2$S not having hydraulic activity at normal conditions. It was found that the model cluster used for calculation can finely reproduce the bulk and surface states using with and without point charge, respectively. The hydration state was also represented by placing OH ̄ on the surface of the cluster. It was calculated that the bond strength of the first layer (as surface) was bigger than that of inner layers (as bulk) for ${\gamma}$-C$_2$S while that of the first layer for ${\gamma}$-C$_2$G was smaller than that of inner layers. Subsequently a model in which OH ̄ is coordinated on Ca at the surface was also calculated. The bond strength with OH ̄ was stronger than that without OH ̄, while for ${\gamma}$-C$_2$G the bond strength with OH ̄ was weaker than that without OH ̄. From these results, it is concluded that the hydraulic activity depends on whether the bond strength for hydrated state becomes weaker than that unhydrated state or not.

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