KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Continuous rapid Production of Soy Sauce by Coimmobilized Mixed Culture system of Zygosaccharomyces rouxii and Candida versatilis using Air Bubble Column Reactor

Zygosaccharomyces rouxii와 Candida versatilis의 동시 고정화에 의한 Air Bubble Column Reactor에서 간장의 연속적 속성 생산

  • 류병호 (경성대학교 식품공학과)
  • Published : 2003.12.01
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Abstract

This study was designed to find out the rapid fermentation of soy sauce from koji hydrolyzates using air bubble column reactor packed with coimmobilized mixed culture system. Continuous ripid production was performed by coimmobilized Z. rouxiii BH-90 and C. versatilis BH-91. Coimmobilized cells of Zygosaccharomyces rouxii BH-90 and Candida versatilis BH-91 mixture cells in the column reactor produced 2.8％ ethyl alcohol and 18mg/L 4-ethylguaiacol over 96 hours under the optimal conditions. Coimmobilized cells produced 2.30∼2.4% ethyl alcohol during 30 days, and decreased gradually from 40 days to 70 days. Also coimmobilized cells produced 4-ethylguaiacol at the constant rate of 16∼18mg/L and decreased gradually after 40 days. Final product of soy sauce contained 2.4% ethyl alcohol and 18mg/L 4-ethylguaiacol. However, amino acid compositions of soy sauce were consisted of predominantly glutamic acid, leucin, arginine, aspartic acid, Iysine and valine, which were more than 50% of total amino acid.

간장의 속성 발효를 위한 방법으로 간장 발효에 간여하는 Z. rouxii BH-90과 C. versatilis BH-91 혼합 균체를 동시 고정화하여 이를 air bubble column reactor에 충진하여 국 고온 분해액을 주입하면서 연속적으로 발효를 시도하였다. 균체의 고정화 담체로는 실리카겔： 3% 알긴산소다 (1:1의 비율) 혼합액을 사용하여 Z. rouxii BH-90과 C. versatilis BH-91을 동시 고정화하여 air bubble column reactor에 충진하여 발효시킨 결과 발효 96시간 경과시 ethyl alcohol이 2.4% 생성되었으며 4-ethylguaiacol은 18mg/L가 생성되었다. Air bubble column reactor에서 Z. rouxii BH-90과 C. versatilis BH-91 혼합균체의 동시 고정화 beads의 연속적 속성 발효시 시작일로부터 30일까지는 ethyl alcohol이 2.5∼2.8%로 거의 일정하게 생성되었고, 40∼70일에는 약간 감소하였다. 그리고 동시 고정화 균체의 발효에서는 40일까지는 4-ethylguaiacol의 함량이 16∼18mg/L로 거의 일정하게 생성되었으나 발효 45일 이후 70일까지는 약간 감소하였다. 발효종료 후의 제품을 분석한 결과 동시 고정화 균체의 발효액에서는 2.4%의 ethyl alcohol이 생성되었고, 18mg/L의 4-ethylguaiacol이 생산되었다. 유리 아미노산의 함량은 glutamic acid, leucine, arginine, aspartic acid, Iysin 및 valine이 전체 아미노산의 50% 이상을 차지하고 있었다.

Keywords

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