Bovine whey protein expression patterns of colostrum are much different from that of milk. Moreover, bovine colostrum is an important source of protective, nutritional and developmental factors for the newborn. However, to our knowledge, no research has been performed to date using a comparative proteomic method on the changes in the bovine whey proteome during the transition from colostrum to milk. This study therefore separated whey protein of days 1, 3, 7 and 21 after calving using two dimension electrophoresis. Differentially expressed proteins at different collection times were identified using high-performance liquid chromatography in tandem with mass spectrometry (LC/MS) and validated by enzyme-linked immunosorbent assay (ELISA) in order to understand the developmental changes in the bovine whey proteome during the transition from colostrum to milk. The expression patterns of whey protein of days 1 and 3 post-partum were similar except that immunoglobulin G was down-regulated on day 3, and four proteins were found to be down-regulated on days 7 and 21 compared with day 1 after delivering, including immunoglobulin G, immunoglobulin M, albumin, and lactotransferrin, which are involved in immunity and molecule transport. The results of this study confirm the comparative proteomic method has the advantage over other methods such as ELISA and immunoassays in that it can simultaneously detect more differentially expressed proteins. In addition, the difference in composition of milk indicates a need for adjustment of the colostrum feeding regimen to ensure a protective immunological status for newborn calves.
Vijayakumar, Mayakrishnan;Park, Ji Hoo;Ki, Kwang Seok;Lim, Dong Hyun;Kim, Sang Bum;Park, Seong Min;Jeong, Ha Yeon;Park, Beom Young;Kim, Tae Il
Asian-Australasian Journal of Animal Sciences
/
v.30
no.8
/
pp.1093-1098
/
2017
Objective: The aim of the current study was to describe the relationship between milk yield and lactation number, stage, length and milking frequency in Korean Holstein dairy cows using an automatic milking system (AMS). Methods: The original data set consisted of observations from April to October 2016 of 780 Holstein cows, with a total of 10,751 milkings. Each time a cow was milked by an AMS during the 24 h, the AMS management system recorded identification numbers of the AMS unit, the cow being milking, date and time of the milking, and milk yield (kg) as measured by the milk meters installed on each AMS unit, date and time of the lactation, lactation stage, milking frequency (NoM). Lactation stage is defined as the number of days milking per cows per lactation. Milk yield was calculated per udder quarter in the AMS and was added to 1 record per cow and trait for each milking. Milking frequency was measured the number of milkings per cow per 24 hour. Results: From the study results, a significant relationship was found between the milk yield and lactation number (p<0.001), with the maximum milk yield occurring in the third lactation cows. We recorded the highest milk yield, in a greater lactation length period of early stage (55 to 90 days) at a $4{\times}$ milking frequency/d, and the lowest milk yield was observed in the later stage (>201 days) of cows. Also, milking frequency had a significant influence on milk yield (p<0.001) in Korean Holstein cows using AMS. Conclusion: Detailed knowledge of these factors such as lactation number, stage, length, and milking frequency associated with increasing milk yield using AMS will help guide future recommendations to producers for maximizing milk yield in Korean Dairy industries.
Geun-woo, Park;Mohammad, Ataallahi;Seon Yong, Ham;Se Jong, Oh;Ki-Youn, Kim;Kyu-Hyun, Park
Journal of Animal Science and Technology
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v.64
no.4
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pp.770-781
/
2022
Meteorological disasters caused by climate change like heat, cold waves, and unusually long rainy seasons affect the milk productivity of cows. Studies have been conducted on how milk productivity and milk compositions change due to heat stress (HS). However, the estimation of losses in milk production due to HS and hereby environmental impacts of greenhouse gas (GHG) emissions are yet to be evaluated in Korean dairy farms. Dairy milk production and milk compositions data from March to October 2018, provided by the Korea Dairy Committee (KDC), were used to compare regional milk production with the temperature-humidity index (THI). Raw data for the daily temperature and relative humidity in 2018 were obtained from the Korea Meteorological Administration (KMA). This data was used to calculate the THI and the difference between the maximum and minimum temperature changing rate, as the average daily temperature range, to show the extent to which the temperature gap can affect milk productivity. The amount of milk was calculated based on the price of 926 won/kg from KDC. The results showed that the average milk production rate was the highest within the THI range 60-73 in three regions in May: Chulwon (northern region), Hwasung (central region), and Gunwi (southern region). The average milk production decreased by 4.96 ± 1.48% in northern region, 7.12 ± 2.36% in central region, and 7.94 ± 2.57% in southern region from June to August, which had a THI range of 73 or more, when compared to May. Based on the results, the level of THI should be maintained like May. If so, the farmers can earn a profit of 9,128,730 won/farm in northern region, 9,967,880 won/farm in central region, and 12,245,300 won/farm in southern region. Additionally, the average number of cows raised can be reduced by 2.41 ± 0.35 heads/farm, thereby reducing GHG emissions by 29.61 ± 4.36 kg CO2eq/day on average. Overall, the conclusion suggests that maintaining environmental conditions in the summer that are similar to those in May is necessary. This knowledge can be used for basic research to persuade farmers to change farm facilities to increase the economic benefits and improve animal welfare.
Objectives : The purpose of this study was to investigate the knowledge of methods to deal with dental trauma in some middle school students. Methods : Among 375 middle school students, a total of 340 filled out the self-administered questionnaire (response rate of 90.7%). The data were analyzed statistically using chi-square analysis. Results : 32.9% of respondents received emergency care education of dental trauma. Knowledge by general information about dental trauma was not so different from the other questions. Tooth storage medium comprised 25.0% and 48.5% in milk and saline, respectively. Conclusions : In order to get the knowledge about emergency treatment of dental trauma in middle school students, it is necessary to develop the professional education program for middle school students.
For the purpose of investigating the weaning practice for infants, this study of 251 infants aged from 3 months to 2 years old was conducted in Daejeon City during the the period of August 9 through 23, 1978. The results obtained were as follows: About 60% of the infants were breast-fed, 27.6%, mixed-fed, and 12.0%, bottled-fed. The reason for bottle-feeding was either the lack of breast-milk secretion or ill-health of mothers. There was no significant difference in the feeding methods according to a level of mother's education. Sixty precent of the infants began to be weaned 6. months. The most of the infants were completely weaned within 18 months. The knowledge on weaning was mainly obtained from the publications, such as newspapers, journals for women, child care booklets, etc. The difficulties in the weaning practice were the shortage of knowledge on weaning and the unacceptability by the infants. Liquid or semi-liquid foods were firstly given to the more than 60% of the infants as introducing weaning foods, while 23.3% of the babies were given solid foods. Interestingly, the earlier the birth order was, the more babies were given liquid or semi-liquid foods, The motives for weaning was either the boby's demand for other foods than milk(41.3%) or the lack of breast-milk secretion (31.3%). In the selection of supplementary foods, the nutritional value was given the highest consideration) (54.9%). many mothers (64.2%) prepared supplementary foods for infants at home, while 23.1% used commercial body foods and 12.7% gave infants adult's meals as supplementary foods. The major materials for home-made supplementary foods were fruits and vegetables (41.5%) and cereals (37.5%). The commercial supplementary foods (mainly powdered from) were not used much (never used; 53.6%) and even in the babies, who had consumed them once, nearly half of the babies (42.9) did not eat well. Accordingly, the many mothers (68.8%) welcomed the development of the domestic supplementary foods in good quality and one-third (31.2%) responded that they would consume them regardless of the price.
We survey recognition of nutrition labeling on the processed foods for the college students with distinction of sex and their major. The frequency of purchase that processed food is over the 3-5 times in a week. The man students preferred to have Ramyon, milk and milk products and the woman students' ingested snacks, soft drinks and sugar snacks. For another, the food major students like to have juice, soft drink, milk and milk products. Then again, the non-food major students ingested Ramyon, snacks and sugar snacks. The woman students and food major students show higher recognition of nutrition labeling and confirmation of it. The man student replied reason why to confirm nutrition labeling is to keep their health. But the woman students show interest to confirm nutrition content. The food major students confirm the nutrition labeling to determine the nutrition labels. The non-food major students did not confirm the nutrition labeling because they think it is an involved style. For knowledge of nutrition contents, the woman student and the student majoring food are well informed. But, all of the student show poor knowledge for staple foods, nutrient function and vitamin. As the research results, we suggest that the educated the student nutrition knowledge for nutrition labeling on the products. It helps to improve their dietary life and eating habits. And they can avoid buying of the processed foods by habit without confirmation of the nutrition.
Many dairy producers periodically receive information about their bulk tank milk with reference to bulk tank somatic cell counts, standard plate counts, and preliminary incubation counts. This information, when collected over a period of time, in combination with bulk tank mastitis culture reports can become a significant knowledge base. Several guidelines have been proposed to interpret farm bulk tank milk bacterial counts. However many of the suggested interpretive criteria lack validation, and provide little insight to the interrelationship between different groups of bacteria found in bulk tank milk. Also the linguistic terms describing bulk tank milk quality or herd management status are rather vague or fuzzy such as excellent, good or unsatisfactory. The objective of this paper was to develop a set of fuzzy descriptors to evaluate bulk tank milk quality and herd's milking practice based on bulk tank milk microbiology test results. Thus, fuzzy logic based reasoning methodologies were developed based on fuzzy inference engine. Input parameters were bulk tank somatic cell counts, standard plate counts, preliminary incubation counts, laboratory pasteurization counts, non agalactiae-Streptococci and Streptococci like organisms, and Staphylococcus aureus. Based on the input data, bulk tank milk quality was classified as excellent, good, milk cooling problem, cleaning problem, environmental mastitis, or mixed with mastitis and cleaning problems. The results from fuzzy reasoning would provide a reference regarding a good management practice for milk producers, dairy health consultants, and veterinarians.
This study was designed to observe the nutritional knowledge, food behavior, nutritional attitudes and food preference of elderly living in home. Two hundred and thirty elderly were examined on questionnaire. The results of this study are summarized as follows. 1. The average score of their nutritional knowledge was 6.08 (the highest mark was 10.00). The nutrition knowledge score of the elderly live with spouse was higher than those live alone and goes up according to the education degree of elderly and pocket money. The correct answer ratio about a question of geriatric diseases was somewhat higher than another question. 2. The average score of their food behavior was 21.90(the highest mark was 33.00) and the score of the female was higher than that of the male and the college-educated elderly have the highest score. Generally their food behavior was improved by the increasing intake of animal protein and milk. 3. The average score of their nutritional attitude was 11.25 (the highest mark was 15.00) and those of 70~74 age old have the lowest score. And they answered that they are willing to change their food behavior for their health. In this study we expect that the authorities would operate the nutritional education for the prevention of degenerative diseases and the development of the food behavior at a public health center or another groups(no-in-jung) as a part of service for the welfare of elderly. 4. They prefered vegetable foods to animal foods and baking, broiling, and raw foods to the fried foods, and they liked the sweet foods, the fruits, milk and lactic acid beverage as snack or dessert.
Journal of Korean Home Economics Education Association
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v.19
no.4
/
pp.17-36
/
2007
The purpose of this study was to develop health belief model based various milk education program as print education media and apply to elementary, middle, and high schools. The subjects were 432 students(124 elementary, 122 middle, 186 high school students). We designed one group pretest-posttest study model. The data were obtained from pre and post-study with self-administered questionnaires. Before applying this education program, we evaluated the degree of awareness on milk. Their awareness on milk was very low, 35.5% lower elementary, 32.7% higher elementary, 52.5% middle, and 54.3% high school students were answered they don't know the milk well. After they had implemented milk education program, their recognition on milk had changed that milk is nutritious as supplementary food. And their reasons for drinking milk were also changed that 'they want to eat it' in elementary school students, 'they want to be healthier' in middle school students, and 'they want to be taller' in high school students. Their nutrition knowledge score showed a significant increase(p<0.05). As a results. milk nutrition education has improved nutrition knowledge and recognition on milk in elementary, middle, and high school students. To improve their milk eating behaviors, nutrition education programs will have to be continued.
Internet websites are excellent resources for younger people to gain information related to breastfeeding. So in this study, we evaluated the breastfeeding knowledge of university students, as well as the currently available website information on breastfeeding. Among the criteria, the six most important evaluation categories for the breastfeeding information on websites were selected and identified. The study participants completed the modified Williams and Hammer's tool, in order to evaluate their breastfeeding knowledge. An additional tool took into consideration the website breastfeeding information criteria, to evaluate the Korean websites. This 34 item tool was categorized into milk supply, comfort during breastfeeding, health of the baby and mother, social support, presentation of message, and products or financial motivation. Thirty-seven websites related to breastfeeding were evaluated, and the results were then discussed by nursing students. The students in this study were not knowledgeable on breastfeeding management with regard to the expression and storage of breast milk, the prevention and care of sore nipples, or use of breastfeeding aids. The breastfeeding information found on the Korean websites received positive evaluations for milk supply, comfort during breastfeeding, and health of the baby and mother. However, the educational content on social support, the message presentation, and the product areas on websites were rare or required additional information from breastfeeding specialists. In conclusion, based on our empirical survey, we need to design websites and evaluation tools for accurate website information regarding the social and economic benefits of breastfeeding, so that Korean women are better motivated to breastfeed by the information they obtain through the Internet.
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