• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.125-129
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• 2011

Environmental risk assessment (RA) is essential prior to the environmental release of GM crops. RA, however, costs at least 7 to 15 million US dollars and requires several years to complete field tests. Therefore, it is strongly suggested that developers of GM crops must consider all criteria for RA at the beginning stage of the development if it aims for commercialization. Previous review papers have pointed out that the "death valley" for the commercialization of GM crops is the screening stage of early GM events since many candidates are given up due to insufficient data on the molecular characterization of a GM event such as inserted gene's copy number, position of inserted site of a chromosome, flanking sequence of recombinant T-DNA, rearrangement of chromosome, and knock out of endogenous gene of host plant. Recently, Rural Development Administration (RDA) in South Korea has launched a Grand National Project named as "Next Generation of BioGreen 21 Project" from 2011 to 2020 and research funding for the development of global GM crops has been allocated to accelerate the commercialization of GM crops. In this regard, I strongly suggest that researchers involved in the development of GM crops for commercialization must conduct RA by themselves at the screening stage of pre-GM event based on the data for molecular characterization.

• Journal of the korean Society of Automotive Engineers
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• v.7 no.1
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• pp.48-54
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• 1985

One of the most probable reason of the apex seal damage in IR-2500 industrial Wankel (rotary) engine was believed to be the engine detonation. Both analytical and experimental studies were made with a view th find out engine detonation pressure. The stagnation detonation pressure $p_{03}$' was estimated based on the data from IR-2500 engine detonation tests, such as engine firing pressure, state of fresh charge at BDC and polytropic compression exponent. The estimated stagnation detonation pressure for the natural gas fueled IR-2500 engine was in excess of 3,700 psia. With natural gas liquid added to the natural gas the octane value of the fuel was lowered, thus, making the engine more prone to detonate. The estimated detonation pressure for the case with the mixed fuel was about 3,400 psia which was sufficiently high to break the apex seal. The subsequent engine lab tests performed on two identical engines with sole difference in the apex seal thickness between the two engines proved that the engine knock, in fact, was the villain of the apex seal failure.ilure.

• BMB Reports
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• v.36 no.1
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• pp.49-59
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• 2003

Platelet-derived growth factor (PDGF) is a critical regulator of mesenchymal cell migration and proliferation. The vital functions of PDGFs for angiogenesis, as well as development of kidney, brain, cardiovascular system and pulmonary alveoli during embryogenesis, have been well demonstrated by gene knock-out approaches. Clinical studies reveal that aberrant expression of PDGF and its receptor is often associated with a variety of disorders including atherosclerosis, fibroproliferative diseases of lungs, kidneys and joints, and neoplasia. PDGF contributes to cancer development and progression by both autocrine and paracrine signaling mechanisms. In this review article, important features of the PDGF isoforms and their cell surface receptor subunits are discussed, with regards to signal transduction, PDGF-isoform specific cellular response, and involvement in angiogenesis, and tumorstromal interactions.

• Childhood Kidney Diseases
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• v.19 no.2
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• pp.79-88
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• 2015

Neutrophil gelatinase-associated lipocalin (NGAL) has emerged as one of the most promising biomarkers of renal epithelial injury. Numerous studies have presented the diagnostic and prognostic utility of urinary and plasma NGAL in patients with acute kidney injury, chronic kidney disease, renal injury after kidney transplantation, and other renal diseases. NGAL is a member of the lipocalin family that is abundantly expressed in neutrophils and monocytes/macrophages and is a mediator of the innate immune response. The biological significance of NGAL to hamper bacterial growth by sequestering iron-binding siderophores has been studied in a knock-out mouse model. Besides neutrophils, NGAL is detectable in most tissues normally encountered by microorganisms, and its expression is upregulated in epithelial cells during inflammation. A growing number of studies have supported the clinical utility of NAGL for detecting invasive bacterial infections. Several investigators including our group have reported that measuring NGAL can be used to help predict and manage urinary tract infections and acute pyelonephritis. This article summarizes the biology and pathophysiology of NGAL and reviews studies on the implications of NGAL in various renal diseases from acute kidney injury to acute pyelonephritis.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.05a
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• pp.730-733
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• 2004

본 연구에서는 넉아웃 옵션(Knock-out option)이 내재된 주가연계상품(ELS)의 성과를 시뮬레이션을 이용하여 추정한다. 옵션과 기초자산을 결합하여 구성되는 ELS는 상품개발 시점에서 그 수익구조가 결정되며, 실현수익률은 미래의 시장흐름에 의하여 결정된다. 현재 ELS는 옵션가격의 결정, 수익구조의 결정, 그리고 수익률 추정이라는 개별 과정이 각각 옵션발행자, 상품개발자, 고객관리자 등에 의하여 별도로 이루어지고 있는 실정이다. 본 연구에서는 이러한 개별 과정을 통합한 시뮬레이션 모형을 구축한 후, 이 모형의 결과(옵션가격, 수익구조, 실현수익률)를 기존 관행의 결과와 비교하여 본 연구에서 제안한 시뮬레이션 모형의 유용성을 제안한다. 분석 대상은 국내 장외파생상품 및 ELS의 기준이 되는 KOSPI 200 지수로 1990년 1월 3일부터 2002년 12월 30일까지의 1일 자료를 이용한다.

• Journal of Microbiology and Biotechnology
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• v.20 no.6
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• pp.1006-1010
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• 2010

Pseudomonas putida JM37 metabolized glyoxylate at a specific rate of 55 g/g dry biomass/day. In order to investigate their role, three genes encoding enzymes that are potentially involved in the conversion of glyoxylate were disrupted; namely, tartronate semialdehyde synthase (gcl), malate synthase (glcB), and isocitrate lyase (aceA). Strains with transposon insertion in either of these genes were isolated from a 50,000 clone library employing a PCR-guided enrichment strategy. In addition, all three double mutants were constructed via targeted insertion of a knock-out plasmid. Neither mutation of gcl, glcB, and aceA nor any of the respective double mutations influenced glyoxylic acid conversion, indicating that P. putida JM37 may possess other enzymes and pathways for glyoxylate metabolism.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.2
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• pp.111-120
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• 2013

Prolyl 4 hydroxylases (P4H) are iron- and 2-oxoglutamate-dependent dioxygenase enzymes and hypoxia-inducible transcription factor (HIF)-P4Hs play a critical role in the regulating oxygen homeostasis in the local tissues as well in the systemic circulation. Over a period of time, a number of prolyl hydroxylase inhibitors and activators have been developed. By employing the pharmacological tools and transgenic knock out animals, the critical role of these enzymes has been established in the pathophysiology of number of diseases including myocardial infarction, congestive heart failure, stroke, neurodegeneration, inflammatory disease, respiratory diseases, retinopathy and others. The present review discusses the different aspects of these enzymes including their pathophysiological role in disease development.

• KSBB Journal
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• v.27 no.2
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• pp.127-130
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• 2012

In this study, the effects of pH, temperature, IPTG concentration and substrate (MSG) concentration on gamma-aminobutyric acid (GABA) production in engineered Escherichia coli were investigated. Glutamate decarboxylase and glutamate/GABA antiporter were overexpressed in GABA aminotransferase knock-out strain for GABA production. The result of optimization study showed the GABA bioconversion was optimized at pH 3.5, $30^{\circ}C$, 0.5 mM IPTG, 10 g/L MSG. At this condition, 5.23 g/L of final GABA concentration of was achieved from 10 g/L of MSG, which corresponded to a GABA yield of 85.77%.

• Proceedings of the Korea Society of Costume Conference
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• 2003.10a
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• pp.75-75
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• 2003

The distinctive design of apparel product is the crucial factor that provides the powerful competitive advantage and customer satisfaction. However, knock-offs or copycats easily copy the design of apparel product and its problem tends to be assented in real world. Design right is one of intellectual property rights that would be produced by intellectual activity and the appropriate means to establish the exclusive rights to the design should be established. Legal protection to the design right is the system that can prelude copycats from eroding an exclusive position in the market place. The present study was to analyze the trend of registration of design rights in apparel products and to point out problems of current protection law to design.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.797-801
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• 2003

The mechanical properties such as shear strength and the hardness of milli-size products that manufactured for various process parameters by forward extrusion using square dies are investigated. Shear strength test is implemented for the observation of relation between vickers hardness and shear strength in the interface of head and shaft part of a stepped pin. When the extrusion ratios of pure aluminum and pure copper billets increase, the hardness on both the surface and the center line of a pin also increase, especially the hardness on the surface is shown to be a little higher than on the center. The existence of knock-out pad in extrusion die caused hardness increase in the interface of a extruded pin. As compared shear strength with hardness of a pin, the approximated linear relations are suggested in this study.