• Title/Summary/Keyword: Km value

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Enzymatic Properties of Fast-migrating Cationic Peroxidase Isozyme from Rice Callus

  • Yoo, Kyung-A;Lee, Mi-Young
    • Journal of Plant Biotechnology
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    • v.4 no.1
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    • pp.39-44
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    • 2002
  • The fast-migrating cationic peroxidase isozyme, named RC3, was purified from rice (Oryza sativa cv. Nak-Dong) callus. Purification of the enzyme was accomplished by ammonium sulfate fractionation, CM-cellulose ionexchange chromatography, and Sephacryl S-100 gel filtration. The molecular mass of the enzyme was about 34 KDa as determined by SDS-PACE and 38 KDa by Sephacryl-100 gel filtration. The pI value of the enzyme was 8.9. Antiserum against RC3 was raised in rabbits, and anti RC3 antiserum reacted with RC3 isozyme by Ouchterlony double immunodiffusion. The optimum pHs and Km values of the enzyme for various substrates were determined. Kinetic studies with various substrates showed that RC3 had very low Km value of 0.01 mM for ferulic acid and ascorbic acid. However, the enzyme did not use esculetin as a substrate.

A Study on Evaluating Length Limit in Tangent Section of Highway Based on Driver's Workload (운전자 작업부하를 고려한 최장 허용 직선길이 결정에 관한 연구)

  • 정봉조;강정규;김주영;장명순
    • Journal of Korean Society of Transportation
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    • v.20 no.2
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    • pp.17-26
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    • 2002
  • Driver's psychophysiological load is one of the key measures for evaluating the safety of the highway. The purpose of this study is to propose and to test the methodology of evaluating the length limit of tangent section using driver's psychophysiological load. Driver's psychophysiological data is represented by the data acquire by frontal and occipital lobe. In order to compare the differences between tangent segments and the orders, real road driving experiments were performed. We collected psychophysiological data during the operation of vehicles. The experimental data were analyzed using FFT (Fast Fourier Transform) and relative power spectrum tools. These routine produces the beta value which is a major factor in consideration of driver's condition. The results in this study are summarized as follows: (1) A new methodology of evaluating the length limit in tangent section of highway using driver's psychophysiological load was proposed. (2) It was observed that driver's work load at tangent section was three times lower than that at the other section types. The beta value at tangent section is 2.219, while that at general section is 0.821. (3) It was observed that the driver's work load was significantly dropped to 0.428 after the continuous driving of 4.2km tangent section. (4) Based on the experimental subjects(from 27 Years to 31). we suggest that 30 times of design speed(3.0 km) could be acceptable as the length limit of tangent section in highway rather than the Previous limit which is 20 times of design speed(2.0km).

The Flexible Design of 0.96 Tbps WDM System over 1,000km NZ-DSF by Using Optimal Parameters of Optical Phase Conjugator (광 위상 공액기의 최적 파라미터를 이용한 1,000km NZ-DSF를 갖는 0.96 Tbps WDM 시스템의 유연한 설계)

  • Lee, Seong-Real;Doh, Kyu-Bong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.7A
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    • pp.657-665
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    • 2007
  • In this paper, it is investigated that the limitation due to the asymmetry of optical power and chromatic dispersion with respect to optical phase conjugator (OPC) for compensating optical signal distortion of WDM channels in mid-span spectral inversion (MSSI) technique is overcame by using OPC position offset and optimal dispersion coefficients of fiber sections, which depend on OPC position offset. It is confirmed that overall WDM channels are efficiently compensated by applying the optimal parameter values obtained from the proposed method into 24 channels ${\times}\;40\;Gbps$ WDM system with non zero - dispersion shifted fiber (NZ-DSF) of 1,000 km, such as power penalties of inter-channel are reduce to almost 3.5 dB from the infinite value. It is also confirmed that the flexible design of WDM system with OPC is possible by using the optimal parameters, in which OPC is placed at ${\pm}15\;km$ from 500 km for efficiently compensating overall channels.

Development of Methods for Estimating Sediment Yield Rate(II) - Development of Models - (비유사량(比流砂量) 추정방법의 개발(II) - 모형 개발 및 검토 -)

  • Kim, Chang Wan;Kim, Hyoung Seop;Yu, Kwon Kyu;Woo, Hyo Seop
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.1
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    • pp.131-140
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    • 1993
  • The major objective of this study is to develop practical methods for estimating sediment yield rates of medium size watersheds of which areas range from 200 to $2,000km^2$ In the first phase of the study that were presented in the companion paper followed by this paper, a methodology for estimating sediment yield rate was introduced and a total of 13 data points including eight sampled river-sediment data and five reservoir deposit data were collected. In this study, a three-parameter empirical model and a six-parameter rating model, both of which are based on empiricism, have been developed. By limited comparisons, the models developed in this study appear to be more reliable and applicable than the existing ones. According to the sediment yield data collected and the estimations by the models, meanwhile, the lowest value for the sediment yield rate of medium size watersheds in Korea is estimated to be about $100tons/km^2/yr$, and the maximum to be about $1,000tons/km^2/yr$.

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Utilization of Korean Medicine among Children with Cerebral Palsy - Qualitative Study by Grounded Theory (뇌성마비 아동의 한방의료 이용행태 - 근거이론에 의한 질적연구)

  • Lee, Hyunjoo;Kim, Buyoung;Yun, Youngju
    • Journal of Society of Preventive Korean Medicine
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    • v.23 no.2
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    • pp.25-42
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    • 2019
  • Objectives : The purpose of this study was to identify factors that affect the utilization of Korean medicine (KM) among children with cerebral palsy (CP) and explore a processing model of decision-making by their parents. Methods : We conducted an individual in-depth interview with 21 mothers of children with CP who were recruited during the observational study. A grounded theory of Strauss and Corbin was used for qualitative analysis. Thus, collected data were classified into open coding including 121 concepts, 27 sub-categories and 7 categories. Results : The central phenomenon of the process of using KM for children with CP is 'KM as an optional treatment not essential'. Therefore there are many obstacles to starting KM treatment and it is easily interrupted for various reasons, unlike general rehabilitation treatment. However, if the patient experiences the therapeutic effect, the parents want to continue KM treatment. They try to provide treatment as much as possible if they can afford it. The parents give priority to general rehabilitation treatment and value the information and experience provided by the caregivers in similar situation as well as expert opinions. Conclusions : To expand and generalize KM treatment for children with CP, efforts to change interventional conditions such as treatment effect, treatment cost, treatment compliance, and convenience of treatment based on the understanding of the strategy used by the parents in KM utilization.

A Study on the Improvement of Accuracy in Mapping the Distribution of the Emission Volume of Air Pollution Using GIS (GIS를 이용한 대기오염 배출량 분포도의 정확도 향상에 관한 연구)

  • 최진무
    • Spatial Information Research
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    • v.6 no.1
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    • pp.65-76
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    • 1998
  • Air contaminant density must be inferred exactly to manage air pollution. Each land use of air pollution source is duplicated in the existing air contaminant distribution because the resolution of the land use map is low. The purpose of this study is to understand how the land use map is used to determine effectively in the distribution calculation of the emission volume and the inference of air contaminant density, as it is made in a high resolution. The major findings are as follows : In this study, as to making a high resolution($28.5m{\times}28.5m$) map of land use with GIS, each air pollution source is not duplicated spatially and land use can be reflected effectively. In Seoul, each air contaminant density was inferred (using a TCM-2 model) with the existing distribution map of emission volume, whose resolution is $1km{\times}1km$, and the new distribution map of emission volume, whose resolution is $28.5km{\times}28.5km$. According to the result, the inference value of the new distribution map was more similar to the actual value of an automatic survey network.

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Comparison of link span dispersion compensation for optical 40 wavelength division multiplexing channels at 2000 km transmission (파장다중화 40 채널 광신호들의 2000 km 전송에서 링크구간 분산보상)

  • Choi, Bo-Hun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.7
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    • pp.1747-1753
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    • 2013
  • Signal performances according link span dispersion compensation conditions at the 2000 km long-haul transmission distance using 10 Gbps 40 wavelength division multiplexing channels were analyzed and compared. 95%, 97.5%, 100%, 102.5% and 105% compensation conditions were applied and 97.5% gave the best performance without post dispersion compensation. the effects of accumulated dispersion value and average dispersion value per transmission link for each link compensation condition were analyzed. When post dispersion compensation optimization was applied to five link span dispersion compensation conditions, 102.5% was given the best performance.

Analysis on the Actual Conditions of Wastewater Treatment Facilities in Chungcheongnam-do Province Industrial Complexes (충청남도 산업단지의 오·폐수처리실태 분석)

  • Lim, Bong-Su;Kim, Do-Young;Yi, Sang-Jin;Oh, Hye-Jung
    • Journal of Korean Society on Water Environment
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    • v.23 no.6
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    • pp.850-862
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    • 2007
  • This study was carried out to survey the actual conditions of wastewater treatment facilities to obtain basic data for the management of wastewater from industrial complexes in Chungcheongnam-do province. Wastewater production flow per site area by watersheds was $49.2m^3/km^2/d$ for Sapgyoho, $8.1m^3/km^2/d$ for Anseongcheon, $5.7m^3/km^2/d$ for Seohae, and $2.9m^3/km^2/d$ for Geumgang. Sapgyoho showed 75% of the total production flow, which was the highest value, Geumgang showed 4% of total flow, which was the lowest value. Average total extra rate as production flow/capacity flow in the wastewater treatment facilities for industrial complex is 49%. Considering by watersheds, the extra rates of Seohae, Geumgang, Anseongcheon, and Sapgyoho, are 73%, 65%, 62%, and 33% respectively. This means that the design of capacity flow in wastewater treatment facilities was too large. Effluent concentration of wastewater treatment facilities did not exceed discharge limit mostly. The removal efficiency rate for water quality item was 90% in BOD, 70% in COD, 80% in SS, 30 to 80% in TN, and 20 to 90% in TP, so the organic removal was good, but the nutrient removal was low and interval of variation was high. The removal efficiency rate of the agricultural was industrial complexes is lower than the national and local complexes. The construction cost of the wastewater treatment facilities in Chungcheongnam-do was $1,756Won\;per\;m^3$, treatment cost was $189Won\;per\;m^3$, and they were about two times and 1.2 times higher than the nation-wide cost, respectively. The treatment cost consists of 39% for man power, 21% for chemical, 16% for power, 11% for sludge treatment, and 13% for others.

Effect of Quizalofop-Ethyl on Glutathione-S-Transferases and Carboxylesterase Activity of Soybean and Corn Plants (Quizalofop-Ethyl이 콩과 옥수수의 Glutathione-S-Transferases와 Carboxylesterase의 활성에 미치는 영향)

  • Kim, Hee-Kwon;Kim, Myoung-Seok;Park, In-Jin;Shu, Yong-Tack
    • Korean Journal of Environmental Agriculture
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    • v.16 no.4
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    • pp.365-372
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    • 1997
  • Biochemical characteristics and activities of glutathione-S-transferases(GSTs) and carboxylesterase extracted from soybean and corn plants treated with quizalofop-ethyl were investigated. Km value and Vmax of GSTs extracted from soybean and corn plants were $6.7{\times}10^{-3}M$ nmole/mg/min, 50, 20 nmole/mg/min, respectively. Optimum pH of carboxylesterase from soybean and corn was 7.0. Km value and Vmax of carboxylesterase extracted from soybean and corn plants were $4.2{\times}10^{-4}M$, $2.5{\times}10^{-4}M$ nmole/mg/min, 33, 10 nmole/mg/min, respectively. GSTs and carboxylesterase activity were reduced by quizalofop-ethyl. GSTs and carboxylesterse activity of corn was more reduced than that of soybean. When soybean and corn were treated by 80 ppm of quizalofopethyl. Soybean recovered after 10 days elapsing, but corn withered after 3days elapsing.

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Properties of Kaolinite as a Raw Material for Porcelain

  • Kim, Jong-Young;Hwang, Kwang-Taek
    • Journal of the Korean Ceramic Society
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    • v.56 no.6
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    • pp.566-576
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    • 2019
  • In this work, we investigated kaolinite raw materials from China, Vietnam, and Seongju (Korea) used for the manufacture of porcelain products in Korea. According to chemical analysis results, for the kaolinites from Seongju, more feldspar components containing CaO, K2O, and Na2O are found than in those from China or Vietnam, of which the composition is close to that of pure kaolinite. Through X-ray Rietveld quantitative analysis, kaolinite and muscovite are found to be the main phases for all the raw materials. Halloysite, quartz, gibbsite, and andesine are found at more than 5% for KM-85 (China), LKC-60 (Vietnam), KC-009 (China), and WA (Seongju). Absorption rate is on order of LKC-60 < KC-009 < KM-85 < PA < WA, and the sample, sintered in reductive atmosphere, exhibits the lowest absorption rate. Comparing the color of the sintered samples, kaolinites of KM-85, LKC-60, and KC-009 (L*: 94 - 97%) show higher whiteness value than those of WA and PA (L*: 82 - 91%).