Monthly changes in the gonadosomatic index (GSI) and hepatosomatic index (HSI) of wild river puffer Takifugu obscurus, and water quality environment in spawning area during breeding season were investigated from March 1995 to February 1996. Monthly changes in GSI and HSI of T. obscurus, that was cultured in low salinity, were calculated. The external morphology of the gonads, germ cell differentiation during gametogenesis and the reproductive cycle with the gonad developmental phases were investigated by histological analysis. The optimum water quality environment in Ganggyung, Choongcheongnam-do, where is spawning ground of wild T. obscurus, was $15-20^{\circ}C$ (water temperature) and 0 psu (salinity). Monthly changes in the GSI in females and males reached a maximum in May, and then rapidly decreased. Therefore, it is assumed that in the natural condition the spawning period of wild T. obscurus is May to June. In females and males, it showed a negative correlationship between the GSI and HSI. The external morphology of the gonads in female and male T. obscurus, that was cultured in low salinity, is composed of a pair of saccular structure. Based on monthly changes in the GSI, it is assumed that in female T. obscurus, that was cultured in low salinity, spawn from March through May. Therefore, it showed a negative correlationship between changes in the GSI and HSI. On the whole, in females and males, it showed a similar pattern between wild and cultured T. obscurus. The reproductive cycle with the gonad developmental phases can be classified into successive five stages in females: the early growing stage, late growing stage, mature stage, ripe and spent stage, and recovery and resting stage. In males, that can be divided into successive four stages: the growing stage, mature stage, ripe and spent stage, and recovery and resting stage. In case of wild T. obscurus, the spawning period has once a year, however, those cultured in the high water temperature ($20-27^{\circ}C$) - low salinity (under 3.3 psu) condition have reproductive characteristics having possibilities of discharge of eggs and sperms year-round as a multiple spawner.
Lee Chung-Hee;Sung Young-Hee;Jung Yoen-Yi;Kim Jung-Suk
The Journal of Korean Academic Society of Nursing Education
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v.4
no.2
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pp.249-263
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1998
The competence of newly graduated nurses is based on various clinical expriences gained when they were students. Therefore, instructors of nursing students, professors in nursing schools or directors in hospitals must play a critical role in assisting them to obtain various knowledge and experienced nursing skills. The purpose of this study was to investigate nursing care activities and nursing care hours practiced by nursing students in a general hospital. The subject students were total 214 nursing students, 2nd graders(sophomores) and 3rd graders(juniors) from 5 Junior Nursing Colleges in Seoul and they practiced at S general hospital to gain clinical experience. The data were collected for 4 days. The tools for this study were the direct nursing care activity list consisted of 15 nursing areas and the indirect nursing care activity list consisted of 9 nursing areas. The subject students were supposed to record their own score. The results of this study are ; 1. The nursing care hours per nursing student 1) The average total nursing care hours a day per each nursing student are 362.65 mins(6.04hr), the direct nursing care hours per each nursing stuent are 202.09 mins(direct nursing care rate 56.0%) and it is higher than the indirect nursing care hours, 159.75mins(indirect nursing care rate 44.0%). The direct nursing care rate of each student by a team approach in the evening shift(56.3%) is higher than that in the day shift(55.8%). 2) The hours of checking vital signs are the longest(47.35mins) among the direct nursing care activites and next is in order of counseling 8l emotional support, nurse rounds, and accompaning a patient during examination. The hours of reporting are the longest(32.39mins) among the indirect nursing care activites, and next is the activities related to education such as reviewing chart, looking up references, etc. 3) The freqency of checking vital signs practiced by nursing student is the highest(the average of 55.7 times) among the direct nursing care activities and next is in order of nurse rounds, assistance of feeding, and counseling & emotional support. The required time for nursing students to accompany their patient during examination is the longest(20.7mins) and next are in order of restriction on patient' activity, orientated by a head nurse, skin care, sitz bath, bathing & hair shampoo, and assisting with patients' exercise. 2. The nursing care hours per grader 1) The average hours of total nursing care per a nursing student are 369.2mins(6.2hrs) to 2nd graders, 355.9mins(5.9 hrs) to third graders. The direct nursing care rate per each nursing student to 3rd graders(59.3%) was higher than that to End graders(52.8%). 2) For 2nd graders, the highly marked nursing activities are teaching associated with direct nursing care activities such as drawing up papers, looking up references, reviewing charts, and being orientated by staff nurses. For 3rd graders, measurments, observations, and nurse rounds in indirect nursing care activities are taken highly 3) The most frequent practice of the nursing care activites is checking vital signs : 65 times to 3rd graders and 46.5 times to 2nd graders. Our suggestions based on the results of this study are : 1. It is recommanded to repeat the same designed study in a variety of clinical fields for further study. 2. It is recommanded to collect data not by self-record method but by observated method. 3. It is needed for nursing instructors in Nursing Schools and in hospitals to develop the guidelines and check-list of clinical practice courses.
Recently, copy number variations (CNV) of genes or genomic segments have been intensively studied and various analysis methods have been developed. In this study, quantitative oligonucleotide ligation assay (qOLA) was applied to investigate CNV of KIT gene in the Landrace breed. A combined assay using qOLA and pyrosequencing, 6 genotype classes, I1/I1 or I3/i (IBe), I1/I2 or I3/IP, I1/I3, I1/IP or I2/i (IBe), I2/I2and I2/IP, were identified from 44 Landrace pigs. Genotype assignment using grouping features of measurements on a scatter plot showed 100% agreement with those using a statistical assignment by PROC FASTCLUS procedure implemented in the SAS package. Two versions (3100 and 3130) of ABI sequencers gave the same genotyping results, indicating there was no influence on qOLA by different versions of instrument, however, the means of standard deviation and coefficient of variation from the qOLA on a ABI 3130 (2.33 and 4.10) was lower than those from the qOLA on a ABI 3100 (2.67 and 4.81). Effect of proteinase K treatment on the PCR product followed by qOLA was very clear because noise peaks were disappeared and the observed ration fit better to the reference ratio corresponding to each genotype.
This study was conducted to investigate the changes in saponin content and antioxidant activity of crude ginseng and extruded ginseng by using different solvent extraction methods. Each of the fractions was first extracted by 80% ethanol followed by ether treatment to remove the lipid components. Water soluble components were separated by ethylacetate and water saturated butanol. Four fraction, including 80% ethanol, ethylacetate, butanol and water were obtained from crude and extruded ginsengs to analyze saponin content and antioxidant activity. Saponin content and antioxidant capacity of each of the four fractions were measured by LC/MS analysis and ORAC(Oxygen Radical Absorbance Capacity) assay, respectively. It was found that a major portion of saponin was present in ethyl acetate and water saturated butanol fractions. When extracted by 80% ethanol, ginsenoside Rb1 and Rg1 were mostly found in crude ginseng, while ginsenoside Re and Rb1 were detected in extruded ginseng. Even though Rh1 and Rg3 were found in a very small quantity in crude ginseng, there was a significant quantity of both in extruded ginseng when extracted by 80% ethanol. Similar tendency was also observed in extruded ginseng fraction when extracted with ethyl acetate and butanol. In crude ginseng, the level of Rg1 was the highest among other ginsenosides upon extraction by ethyl acetate, while Rh1 and Rg3 were predominantly found by employing similar solvent extraction in the extruded ginseng. Also, Rg1, Re and Rb1 were also found in the extruded ginseng with small quantity. Rg1, Re and Rb1 were found in crude ginseng by butanol extraction, while Rb1 and Re were extracted from the extruded ginseng. Overall, there was no difference in the saponin content between crude ginseng and extruded ginseng when extracted by butanol and water, but twice as much of saponin was obtained by 80% ethanol extraction and 6 times more saponin were obtained in ethyl acetate fraction in the extruded ginseng. Antioxidant capacity of crude ginseng as determined by ORAC assay was higher in 80% ethanol(high in many different kinds of biological compounds) and water saturated butanol(high in polar saponin) fractions than the ethyl acetate and water fractions. No difference in antioxidant capacity was observed between crude and extruded ginseng. However, antioxidant capacity of ethyl acetate and water fractions in extruded ginseng was significantly higher than crude ginseng($P$ >0.05). All the fractions in both, crude and extruded ginseng possessed antioxidant capacity and even water fractions that contained almost no saponin had some antioxidant capacity. While determining correlation coefficient between fractions in extruded ginseng by Pearson correlation, it was observed that 80% ethanol fraction was in correlation with ethyl acetate($P$ >0.01) and ethanol($P$ >0.001) and in the case of ethylacetate, correlation was observed only with butanol fraction($P$ >0.05).
Objectives : This study examined the selective deficits of executive function in patients with mild traumatic brain injury that in normal range of general intelligence level and aimed to analysis of the correlation between K-WAIS result and executive function. Methods : 59 subjects were included in this study, who were diagnosed as mild traumatic brain injury(MTBI) and they have visited in neuropsychiatric department of Wonkwang University Hospital during from March, 2005 to September, 2010. For measurement of general intelligence quotient, the Korean-Wechsler Adults Intelligence Scale(K-WAIS) was administered and for measurement of executive intelligence quotient(EIQ), Executive Intelligence Test(EXIT) was administered. Results : Of patients, 50.8% included at abnormal EIQ group. The patients of abnormal EIQ showed poorer full scale IQ(FIQ), performance IQ(PIQ) and in subscale that picture arrangement, digit symbol, digit span, block design, object assembly and comprehension were significantly different. In terms of relationships between K-WAIS and EIQ, FIQ and PIQ have positive correlation with EIQ. And in subscale, picture arrangement, digit symbol, digit span, block design, object assembly and comprehension show positive correlation with EIQ. Conclusion : This study suggest that MTBI patients with have normal range of general intelligence level may have deficit of executive function is common. The decline of FIQ, PIQ and some subscales of K-WAIS may suggest executive dysfunction in MTBI patients.
This experiment was carried out to investigate the effects of physico-chemical properties of soil on the growth of Chamaecyparis obtusa and to apply the results to such rational forest management as yield forecast and selection of suitable species for a given forest stand. The soil properties observed in this experiment were soil pH, exchangeable pH organic matter, total nitrogen, available $P_2O_5$, cation exchange capacity, exchangeable $H^+$, total base and base saturation. Diameter at breast height (DBH), height and volume growth of C. obtusa were observed at 78 sampling sites. Data were processed into the following series for the analysis of multivariates : inner correlation matrix, multiple correlation coefficients, regression coefficients and partial correlation coefficients. The results are summerized as follows : 1. Multiple correlation coefficients between soil properties and DBH of C. obtusa were .364 for 20-year trees, resulting less efficient for estimating the growth of C. obtusa. However, total base, soil pH and base saturation were considerable characters for better estimation. 2. More efficient multiple correlation coefficients were observed between soil properties and height growth than those between soil properties and DBH, showing .443 for 20-year trees and factors affecting the height growth were similar to those of DBH. 3. Extremely low values of multiple correlation coefficients were estimated between physico-chemical properties of soil and volume growth of C. obtusa resulting low efficient estimation. However, total base contributed highly to volume growth of C. obtusa. Accordingly the most contributable factor for estimating the growth of C. obtusa were total base, soil pH and base saturation. Consequently, these results can be effective for selecting of the reforesting site, and less effective for estimating the growth of C. obtusa.
Kim, Ji-Hye;Jeon, Jin-Yong;Heo, Yu-Ri;Son, Mee-Kyoung
The Journal of Korean Academy of Prosthodontics
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v.51
no.4
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pp.269-275
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2013
Purpose: The aim of this retrospective study was to evaluate the influence of implant diameter, length and placement to implant stability. Materials and methods: Total 90 implants (US II plus$^{TM}$, Osstem co, Busan, Korea) of 72 patients were determined as experimental samples. The factors of diameters(${\phi}$ 4 mm, ${\phi}$ 5 mm), lengths (10 mm, 11.5 mm, 13 mm), and implant placement (maxilla, mandible) were analyzed. The stability of the implants was measured by resonance frequency analysis (RFA) at the time of implant placement and impression taking. The difference of ISQ values according to patient's gender was evaluated by Independent t-test. ISQ values were compared between implant diameter, length and placement using one-way ANOVA and Tukey HSD test (${\alpha}=.05$). To compare ISQ values between at the time of surgery and impression taking, paired t-tests were used (${\alpha}=.05$). Results: The change of implant length did not show significant different on the ISQ value (P>.05). However, 5 mm diameter implants had higher ISQ values than 4 mm diameter implants (P<.05). Implants placed on the mandible showed significantly higher ISQ values than on the maxilla (P<.05). Conclusion: In order to increase implant stability, it is better to select the wider implant, and implants placed on mandible are possible to get higher stability than maxilla. ISQ values at impression taking showed higher implant stability than ISQ values at implant placement, it means that RFA is clinically effective method to evaluate the change of implant stability through the osseointegration. The consideration of the factors which may affect to the implant stability will help to determine the time of load applying and increase the implant success rate.
Korean Journal of Agricultural and Forest Meteorology
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v.15
no.3
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pp.171-177
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2013
The purpose of this study is to find a scheme to scale down the KMA (Korea Meteorological Administration) digital precipitation maps to the grid cell resolution comparable to the rural landscape scale in Korea. As a result, we suggest two steps procedure called RATER (Radar Assisted Topography and Elevation Revision) based on both radar echo data and a mountain precipitation model. In this scheme, the radar reflection intensity at the constant altitude of 1.5 km is applied first to the KMA local analysis and prediction system (KLAPS) 5 km grid cell to obtain 1 km resolution. For the second step the elevation and topography effect on the basis of 270 m digital elevation model (DEM) which represented by the Parameter-elevation Regressions on Independent Slopes Model (PRISM) is applied to the 1 km resolution data to produce the 270 m precipitation map. An experimental watershed with about $50km^2$ catchment area was selected for evaluating this scheme and automated rain gauges were deployed to 13 locations with the various elevations and slope aspects. 19 cases with 1 mm or more precipitation per day were collected from January to May in 2013 and the corresponding KLAPS daily precipitation data were treated with the second step procedure. For the first step, the 24-hour integrated radar echo data were applied to the KLAPS daily precipitation to produce the 1 km resolution data across the watershed. Estimated precipitation at each 1 km grid cell was then regarded as the real world precipitation observed at the center location of the grid cell in order to derive the elevation regressions in the PRISM step. We produced the digital precipitation maps for all the 19 cases by using RATER and extracted the grid cell values corresponding to 13 points from the maps to compare with the observed data. For the cases of 10 mm or more observed precipitation, significant improvement was found in the estimated precipitation at all 13 sites with RATER, compared with the untreated KLAPS 5 km data. Especially, reduction in RMSE was 35% on 30 mm or more observed precipitation.
Kim, Min-Kyeong;Kwon, Soon-Ik;Jung, Goo-Bok;Hong, Seong-Chang;Chae, Mi-Jin;Yun, Sun-Gang;So, Kyu-Ho
Korean Journal of Environmental Agriculture
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v.32
no.4
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pp.355-358
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2013
BACKGROUND: Water-born pollution loads by agricultural non-point source (NPS) pollution are expected to become intensified due to ongoing precipitation change. Therefore, it is essential to develop a best management practice (BMP) that is suitable to agricultural environments in Korea. This study aimed to develop an environmental-friendly BMP to reduce NPS pollution load by agricultural activities. An eco-friendly way, small drainage pond, was suggested in this study to avoid direct drainage of agricultural runoffs and eventually reduce the amount of pollutants discharged into the surrounding aqua-environment. METHODS AND RESULTS: A small pond ($12m^2$) was constructed at the corner of a rice paddy field ($1,715m^2$) located in Suwon, Korea. Water was allowed to drain only via a small drainage pond. Sampling was repeatedly made at two locations, one from an entrance and the other from an exit of a pond, during the rice cultivation period (May to October, 2012). Generally, sampling was made only when runoff water drained through a pond, such as during and/or after rain (irrigation). The water quality analysis showed that all quality parameters (SS, $COD_{Mn}$, T-N, and T-P) were improved as water passed through the pond. The amount of runoff water was reduced by 96~100%. Suspended solids and COD concentrations was reduced by 79.3% and 45.6%, respectively. In case of T-N and T-P concentrations, the reduction rates were 52.2% and 60.5%, respectively and the amount of T-N and T-P were reduced by 16.3~73.0% and 15.4~70.1%, respectively. CONCLUSION(S): Our data implies that agricultural NPS pollution from rice paddy fields can be effectively managed when an appropriate drainage water management practice is imposed. In this paper, it was suggested that an installation of a small drainage pond can be effective to prevent not only the nutrient loss from rice fields but also pollutant discharge to surrounding water environments.
The purpose of this study was to investigate the effect of alcoholic drinking on the dietary habits among university students. The data was collected from 355 students (male : 188, female : 167) living in Busan. The questionnaire composed of general information, person with the lunch meal time, skipping meal, meal type place overeating, snacking eating out, food intake pattern. For statistics analysis, SPSSWIN 12.0 was used. The results were summarized as follows. First, the rates of alcohol drinking in male and female students were 94.1% and 93.4% respectively and the rate of under 19 years old's drinking were 94.4%. Second, in the appearance of drinking, the difference in dietary habits wasn't shown to be significant. However in term of dinner, few female students sometimes haven't dinner (p<0.01). And female group showed frequently eating out (p<0.05) and overeating more than male group(p<0.001). Third, the alcohol drinking group and non-alcohol drinking group showed significantly similar consumption frequency of cereal, meat products, fruits vegetable, oil sugars. But the intakes of milk and dairy products in male students were significantly higher than in female students (p<0.001). These results indicated that more attention should be taken to university students, having habits of skipping meal, alcohol drinking and low intake for nutrition knowledge or attitude so as to improve their health.
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