• The Korean Journal of Physiology and Pharmacology
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• v.1 no.1
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• pp.55-63
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• 1997

The present study was designed to quantify the alterations of renal renin, angiotensin type I receptor ($AT_1$), $TGF-{\beta}1$, and fibronectin gene expression in rats with unilateral ureteral obstruction (UUO). We also investigated the change of $AT_1$ density during UUO. Reverse transcription-polymerase chain reaction (RT-PCR) technique and receptor binding assay were used to detect mRNA expression and receptor density, respectively. At one day after UUO, renin mRNA level of the obstructed kidneys was decreased transiently and then subsequently increased to the level of sham kidneys. In the contralateral kidneys of the same rats, on the contrary, renin mRNA level was gradually decreased. Then, at 9 days after UUO, it was significantly lower than that of sham kidneys. The expressions of both $AT_1$ subtypes, called $AT_{1A}$ and $AT_{1B}$, mRNAs did not change at any time. UUO led to a significant decrease in $AT_1$ density in the obstructed kidneys compared with the sham kidneys at 1 and 3 days $(66\;{\pm}\;11.6%\;(p<0.005)\;and\;73\;{\pm}\;4.0%$ (p<0.01), respectively). Thereafter, $AT_1$ density was gradually increased and at 9 days it showed a marked elevation in the obstructed kidneys compared to the sham kidneys. In contrast, in the contralateral kidneys $AT_1$ density was significantly reduced from 3 to 9 days after UUO. The $TGF-{\beta}$1 mRNA level of the obstructed kidneys was unexpectedly decreased at 6 days after UUO. Then, at 9 days it was followed by a significant increase in the obstructed kidneys, whereas it showed an obvious decrease in the contralateral kidneys. In addition, fibronectin mRNA level was also significantly increased in the obstructed kidneys after UUO compared to the sham or the contralateral kidneys of the same rats. These results suggest a differential regulation of renal renin, $AT_1$ receptor, $TGF-{\beta}$1 and fibronectin mRNA levels at different stages of UUO.

• Journal of Korean Medical classics
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• v.29 no.3
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• pp.101-111
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• 2016

Objectives : In this study, the meaning of Movement Qi between kidneys(腎間動氣) in Huangdineijingtaisu(黃帝內經太素) is researched for the purpose of understanding the differences between Huangdineijingtaisu and Nanjing(難經). Methods : The annotations related to Movement Qi between kidneys in Huangdineijingtaisu were selected and analyzed to overview the historical changes of the definition. Results : The meaning of Movement Qi between kidneys in Huangdineijingtaisu is not different from that of Nanjing basically, however, Yang Shangshan(楊上善) suggested additional new meanings including penetration meridian(衝脈), pass way for vital energy(氣街), visceral exhaustion pulse(眞藏脈) and so on. Conclusions : The reason that Yang Shangshan explained the circulation of primordial energy(原氣) focusing on penetration meridian is that he tried to connect the actual functions about Movement Qi between kidneys to clinical treatment than the ideal concept in Nanjing.

• Herbal Formula Science
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• v.32 no.3
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• pp.311-324
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• 2024

Objectives : Human body keeps balance through the interaction of various organs, especially the lungs and kidneys are closely connected in maintaining health and preventing disease. This study explores how the lungs and kidneys interact in terms of breathing and fluid balance and aims to find common ground between Eastern and Western medical practices. Methods : Similar explanations related to the interaction between the lungs and kidneys in the physiology and pathology of Traditional Korean Medicine(TKM) and biomedicine were compared. Results : In breathing, the lungs and kidneys work together by adjusting abdominal pressure with the diaphragm and maintaining acid-base balance, and hormones and enzymes secreted from the kidneys significantly affect lung function. This process corresponds to the concept of TKM that the kidneys control the reception of qi (腎主納氣). For fluid balance, the lungs help manage fluid levels through evaporation and sweating, interacting with the kidneys via the Renin-Angiotensin System (RAS), ACE, ACE2 enzymes, and antidiuretic hormone (ADH). This is similar to the theory in TKM that the lungs regulate human fluid (肺主通調水道). Conclusions : This research shows that by looking at the same physiological and pathological processes from different angles, we can reduce misunderstandings between Eastern and Western medicine. It helps improve the understanding of TKM's theories and supports building a unified framework for both medical traditions. Future work should focus on developing compatible theoretical systems across these fields.

• Journal of Korean Medical classics
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• v.26 no.4
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• pp.253-266
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• 2013

Objective : There was no attempt to understand Moving Energy between two kidneys(腎間動氣) and Kidney on Left & Life Gate on Right(左腎右命門) by integration progress. So I have faced to study based on two parts with concerning as clues. One is 'Life Right (左 右)' and the other is 'Between(間)'. Methods : Revealing the source of the origin, Nanjingbenyi(難經本義) is given on the basis. Take a close look at publications related to Nanjing(難經) which is about Kidney on Left & Life Gate on Right and Moving Energy between two kidneys. Take a close look at Kidney, the Life Gate and Moving Energy between two kidneys. Look see the three-dimensional uplift movement of Gi(氣). Results : In Neijing(內經) and Nanjing, the basic point of view for Kidney is the same. That is explained in line with attributes of convergence(收斂). 'Life Gate(命門)' is a term to express the divergence feature(發散機能) of kidney. Moving Energy between two kidneys is used to mean the mainspring of human body activity. The Gi in human body loses altitude turning left(左旋而下降) and gains height turning right(右旋而上升). Conclusion : Watching on functional aspect, there are two names for kidney. One is 'Kidney(腎)' which collects the losing altitude turning left and the other is 'Life Gate' which rises turning right. Moreover, the fundamental power that effectuate the uplift movement is Moving Energy between two kidneys. This kind model is a way that can be understood syntagmatically the Kidney on Left & Life Gate on Right and the Moving Energy between two kidneys without any gainsaying the original of Nanjing.

• The Journal of Korean Medicine
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• v.22 no.2
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• pp.94-101
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• 2001

Background and Purpose : Relationship between 17-KS.17-OHCS in 24hrs urine and Deficiency Syndrome of the Kidneys had been examined, but the study about 17-KS.17-OHCS in stroke patients was rare6'. In this study, we aimed to investigate the usefulness of 24hrs urine 17-KS.17-OHCS in stroke patients as an index for the Differentiation of Deficiency Syndrome of the Kidneys. Subjects : 66 stroke patients(male : female =2 9 : 37) were selected, they were admitted in the hospital of oriental medicine, Kyunghee university(from November 1 st, 1998 to May 30th, 2000). Their age was over 65 years. The patients who had renal malfunction, hyperthyroidism, hypothyroidism were excluded and who took chlorpromazine, spironolactone, digoxin, reserpine, hormonal agent were also excluded. Methods : After we selected the patients, we investigated the Differentiation of Syndrome by use of Diagnostic Paper and examined the level of 17-KS.17-OHCS in 24hrs urine. We compared Deficiency Syndrome with non-Deficiency Syndrome of the Kidneys using of 17-KS.17-OHCS in 24hrs urine. Results : 1. Stroke did not affect 17-KS.17-OHCS excretion in 24hrs urine. 2. In 24hrs urine, 17-KS of male stroke patients and 17-OHCS of female stroke patients were lower in patients diagnosed as a Deficiency Syndrome than non-Deficiency Syndrome of the Kidneys(p<0.05). 3. Among Deficiency Syndrome of Yin, Yang, Yang and Yin of the Kidneys group, there was no differentiation of 17-KS.17-OHCS in 24hrs urine(p>0.05).

• Journal of Korean Medical classics
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• v.29 no.3
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• pp.113-132
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• 2016

Objectives : In this study, the Abdominal Diagnostic Method of Kitao Shunpo(北尾春圃) about Movement Qi between kidneys in Sanghauidam(桑韓醫談) and Jeonggisinron(精氣神論) are investigated for the purpose of understanding the definite technique of the method and the position in the context of abdominal diagnosis in Japan. Methods : The materials related to the abdominal diagnosis to Movement Qi between kidneys were selected from Sanghauidam and Jeonggisinron written by Kitao Shunpo, and analyzed to know the characteristics of the method. Results : Kitao Shunpo suggested the theoretical background of the Abdominal Diagnostic Method that Original Qi(元氣) is made from Original Essence(元精) when a person is born, and mentality(神) and body essence(精), qi(氣) and blood(血) are formed from those. Movement Qi between kidneys(腎間動氣), that is to say innate Original Qi can be diagnosed in abdominal region by the method in Japanese Medicine. Kitao Shunpo decided deficiency and excess of Original Qi and prognosis in clinical treatment by the method, and suggested the standard pulse condition of the Movement Qi between kidneys. Conclusions : After the theory about Movement Qi between kidneys appeared in Nanjing(難經), many doctors have not made attention to diagnose that for a long time, until Kitao Shunpo with other Japanese doctors established the theoretical background of the Abdominal Diagnostic Method, and applied the theory to clinical treatment.

• Journal of Veterinary Clinics
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• v.18 no.4
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• pp.358-362
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• 2001

Brain dead (BD) patients remain the largest source of solid organs for transplantation. BD has shown to decrease graft function and survival in rodent models. The aim of this study was to evaluate how brain death affects graft viability in the donor and kidney tolerance to cold preservation as assessed by survival in a canine transplantation. 13 Beagle dogs were used for the study. Brain death was induced by the sudden inflation of a subdural balloon catheter with continuous monitoring of arterial blood pressure and eletroencephalographic activity (n=3). Sixteen hours after conformation of brain death, kidney graft were retrieved (n=6). Non-BD donors served as controls (n=4). All kidneys were flushed with University of Wisconsin (UW) solution and preserved for 24 hours at 4$^{\circ}C$ before transplantation. Recipient survival rates, serum creatinine level were analyzed. Brain death induced the well-known Cushing reaction with a severe increase in blood pressure and tachycardia. Thereafter, cardiac function returned progressively to baseline within 8 hours and remained stable until the end of the experiment. All of dogs in both group transplanted were survived until 7 days (100%), and the kidneys showed functional early rejection at 8.3$\pm$0.5 days and 8.5$\pm$0.5 days after transplantation, in BD and allograft group, respectively. BD kidneys were functionally similar to control kidneys for 7 days after transplantated. Brain death has no deleterious effect on preservation injury and survival of dog kidney transplantation, although it induces changes in hemodynamic parameters. This study reveals that kidneys from BD donors do not exhibit more ischemia reperfusion injury, and support good early function and survival.

• YAKHAK HOEJI
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• v.26 no.4
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• pp.253-256
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• 1982

The organ distribution of mercury was examined in the rat after oral administration of a single dose of red mercuric sulfide (15mg Hg/kg). The concentration of total mercury in the organs and blood after 2, 4, 6, 8, 12, 24 and 72 hours of administration was determined by Quartz Tube Combustion-Gold Amalgamation Method. It was found that the maximal concentration of total mercury was in the kidneys and muscle within 24 hours and in the brain, heart, liver and blood within 48 hours. The descending order of the maximal organ and blood concentration was: kidneys(1.08ppm)>blood> muscle>heart>liver>brain. The accumulation states of total mercury in the rat organs were investigated by continuous administration of red mercuric sulfide (5mg Hg/kg/day) for 15 days. The mercury concentration increased progressively throughout the experimental period and the descending order of the highest level of mercury after 15 days was: kidneys (1.55ppm)>blood>liver. The concentration of alkyl mercury in brain, liver and kidneys also was measured after 7 and 15 days of consecutive administration of red mercuric sulfide (5mg Hg/kg/day). The concentration in the Kidneys and the liver was very low, but was significantly different from control group. The concentration in the brain was extremely low and was not significantly different from control group.

• Journal of Environmental Health Sciences
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• v.13 no.1
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• pp.41-46
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• 1987

The purpose of this experiment was to investigate the leisons of mice produced by intraperitoneal injection of rubratoxin B. But the mice injected 3$\mu$g and 5$\mu$g of rubratoxin B showed retardation of growth, and slight swelling of kidneys. The hemorrhage in the renal cortex tubular dilation containing the desquamated epithelial cells, and adhesion of Bowman's spaces by proliferation of endothelial cells were histopathoglogically characterized in the kidneys of mice.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.10
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• pp.1433-1439
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• 2000

An experiment was carried out with 48 IVRI 2CQ rats 6-8 week old, weighing 50-100 g, to study the effect of lead exposure on antioxidant defense, lipid peroxide level, status of thiol groups and concentration of lead in the liver and kidneys at the end of the exposure and also after withdrawal of lead administration. Twenty four rats were given lead at a daily dose rate of 1 mg lead/2 ml of distilled water/kg body weight as lead acetate solution intraperitoneally for a period of 30 days. Another 24 control rats received 2 ml of sterile normal saline solution (0.85% NaCl)/kg body weight in an identical manner. A many-fold increase in concentration of lead was associated with a non-significant (p>0.05) decrease in the activities of superoxide dismutase (SOD) in the liver (27%) and kidneys (12%) and catalase in kidneys (22%). A significant (p<0.05) increase in lipid peroxide level was recorded in the liver (40%) compared with control values. There were significant (p<0.05) decreases in the total thiol and protein bound thiol contents in liver and an increase in non-protein bound thiol groups in the kidneys of lead exposed rats. During the 10 day observation period after withdrawal of lead administration, no significant change was observed with respect to any of the above parameters indicating that a 10 day withdrawal period was not enough for restoration of normality. It is concluded that the magnitude of response and the resultant changes in the lipid peroxide concentration, and the activities of SOD and catalase were not identical in the liver and kidneys of lead-exposed rats.