• Title/Summary/Keyword: KiSS-1

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Chromium(VI) Adsorption Behavior of Silk Sericin Beads

  • Kwak, Hyo Won;Yang, Ye Sol;Kim, Moo Kon;Lee, Jeong Yun;Yun, Haesung;Kim, Min Hwa;Lee, Ki Hoon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.26 no.1
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    • pp.47-53
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    • 2013
  • Silk sericin (SS) has been fabricated into beads using a 1 M LiCl/DMSO solvent and utilized as a heavy metal adsorbent. Among the various heavy metals, we targeted Cr(VI) for adsorption using SS beads and found that its adsorption depended on the coagulant used for the fabrication of the SS beads. When methanol was used as a coagulant, the beads had a better adsorption capacity than when ethanol was used except at pH 1. The adsorption behavior of Cr(VI) on the SS beads followed the BET isotherm. The maximum adsorption capacity was 33.76 mg/g at pH 2. The adsorption of Cr(VI) was confirmed by FT-IR and EDS analyses. Finally, the desorption was carried out using NaOH solution, and it was found that 73.19% of the adsorbed Cr(VI) could be detached.

Enhanced Electrochemical Properties of Dye-sensitized Solar Cells Using Flexible Stainless Steel Mesh Electrodes with Ti Protective Layer (Ti 보호층이 형성된 스테인레스 스틸 메쉬 전극을 이용한 염료감응형 태양전지의 전기 화학적 특성 개선)

  • Jung, Haeng-Yun;Ki, Hyun-Chul;Gu, Hal-Bon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.28 no.3
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    • pp.180-184
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    • 2015
  • Stainless steel (SS) mesh was used to fabricate photoelectrode for flexible dye-seisitzed solar cells (DSSCs) in order to evaluate them as replacements for more expensive transparent conductive oxide(TCO). We fabricated the DSSCs with new type of photoelectrode, which consisted of flexible SS mesh coated with 100 nm thickness titanium (Ti) protective layer deposited using electron-beam deposition system. SS mesh DSSCs with protective layer showed higher efficiency than those without a protective layer. The best cell property in the present study showed the open circuit voltage (Voc) of 0.608 V, short-circuit current density (Jsc) of $5.73mA\;cm^{-2}$, fill factor (FF) of 65.13%, and efficiency (${\eta}$) of 2.44%. Compared with SS mesh based on DSSCs (1.66%), solar conversion of SS mesh based on DSSCs with protective layer improved about 47%.

Advanced Onset of Puberty in High-Fat Diet-Fed Immature Female Rats - Activation of KiSS-1 and GnRH Expression in the Hypothalamus -

  • Lee, Song-Yi;Jang, Yeon-Seok;Lee, Yong-Hyun;Seo, Hyang-Hee;Noh, Kum-Hee;Lee, Sung-Ho
    • Development and Reproduction
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    • v.13 no.3
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    • pp.183-190
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    • 2009
  • In mammals, puberty is a dynamic transition process from infertile immature state to fertile adult state. The neuroendocrine aspect of puberty is started with functional activation of hypothalamus-pituitary-gonadal hormone axis. The timing of puberty can be altered by many factors including hormones and/or hormone-like materials, social cues and metabolic signals. For a long time, attainment of a particular body weight or percentage of body fat has been thought as crucial determinant of puberty onset. However, the precise effect of high-fat (HF) diet on the regulation of hypothalamic GnRH neuron during prepubertal period has not been fully elucidated yet. The present study was undertaken to test the effect of a HF diet on the puberty onset and hypothalamic gene expressions in immature female rats. The HF diet (45% energy from fat, HF group) was applied to female rats from weaning to around puberty onset (postnatal days, PND 22-40). Body weight and vaginal opening (VO) were checked daily during the entire feeding period. In the second experiment, all animals were sacrificed on PND 36 to measure the weights of reproductive tissues. Histological studies were performed to assess the effect of HF diet feeding on the structural alterations in the reproductive tissues. To determine the transcriptional changes of reproductive hormone-related genes in hypothalamus, total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Body weights of HF group animals tend to be higher than those of control animals between PND 22 and PND 31, and significant differences were observed PND 32, PND 34, PND 35 and PND 36 (p<0.05). Advanced VO was shown in the HF group (PND $32.8{\pm}0.37$ p<0.001) compared to the control (PND $38.25{\pm}0.25$). The weight of ovaries (p<0.01) and uteri (p<0.05) from HF group animals significantly increased when compared to those from control animals. Corpora lutea were observed in the ovaries from the HF group animals but not in control ovaries. Similarly, hypertrophy of luminal and glandular uterine epithelia was found only in the HF group animals. In the semi-quantitative RT-PCR studies, the transcriptional activities of KiSS-1 in HF group animals were significantly higher than those from the control animals (p<0.001). Likewise, the mRNA levels of GnRH (p<0.05) were significantly elevated in HF group animals. The present study indicated that the feeding HF diet during the post-weaning period activates the upstream modulators of gonadotropin such as GnRH and KiSS-1 in hypothalamus, resulting early onset of puberty in immature female rats.

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Performance Evaluation of Vortex Screen for Treatment of Fine Particles in Storm Runoff (Vortex Screen장치를 이용한 강우유출수내 미세입자 처리특성 분석)

  • Lee, Jun-Ho;Jung, Yun-Hee;Bang, Ki-Woong
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.4
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    • pp.256-262
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    • 2009
  • The use of hydrodynamic separator is becoming increasingly popular for suspended solids reduction in urban storm runoff. This study is a laboratory investigation of the use of Vortex Screen to reduce the solids concentration of synthesized storm runoff. The synthesized storm runoff was made with water and addition of particles; manhole sediment, road sediment, fly ash, and ployvinyl chloride powder. Vortex Screen was made of acryl resin with 250 mm of diameter and height of 700 mm. To determine the removal efficiency for various influent concentrations of suspended solids (SS) and chemical oxygen demand (COD), tests were performed with different operational conditions. The samples were taken simultaneously at the influent storage tank and effluent tank, and measured SS and COD concentrations. The ranges of surface loading rate were 110 to 1,550 $m^3/m^2$/day, and influent SS concentrations were varied from 141 to 1,986 mg/L. This paper was intended to evaluate the effect of inlet baffle and the ratio of underflow to overflow ($Q_U/Q_O$) on particle separation efficiency for various particle size using Vortex Screen. It was found that when increase of $Q_U/Q_O$ from 10% to 20%, SS removal efficiency was increased about 6%. The range of SS and COD removal efficiencies of road sediment particle size 125<$d_p$<300 ${\mu}m$ were 68.0~81.0%, 53.1~71.9%, respectively. Results showed that SS removal efficiency with inlet baffle improved by about 10~20% compared without inlet baffle.

Waste Treatment by an Anaerobic Filter (혐기성(嫌氣性) Filter Media 반응조(反應槽)에 의한 폐수처리(廢水處理))

  • Lee, Chan Ki;Rim, Jae Myung
    • Journal of Industrial Technology
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    • v.2
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    • pp.33-44
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    • 1982
  • This study mainly concentrated on the treatment of nightsoil by an anaerobic filter media process. Nightsoil has been one of the major water pollutants in this couotry. It represents high ROD and SS concentrations. A comprehensive laboratory study conducted at Kangwoon National University indicate: (1) In the case of anaerobic filter system, COD and BOD removal efficiencies were greatly influenced by hydraulic retention time(HRT), but SS removal efficiencies dropped slowly when HRT were 2~13 days. (2) COD removal efficiencies were 19% when HRT were 15 days, 14% when HRT were 8 days, 10% when HRT were 6 days, 9% when HRT were 4 days, 2% when HRT were 2 days. (3) BOD removal efficiencies were 23% when HRT were 15 days, 14% when HRT were 8 days, 18% when HRT were 6 days, 7% when HRT were 4 days, 5.8% when HRT were 2days. (4) SS removal efficiencies were 21%~25%(2~15 days HRT).

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Regulation of L-type Calcium Channel Current by Somatostatin in Guinea-Pig Gastric Myocytes

  • Kim, Young-Chul;Sim, Jae-Hoon;Lee, Sang-Jin;Kang, Tong-Mook;Kim, Sung-Joon;Kim, Seung-Ryul;Youn, Sei-Jin;Lee, Sang-Jeon;Xu, Wen Xie;So, In-Suk;Kim, Ki-Whan
    • The Korean Journal of Physiology and Pharmacology
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    • v.9 no.2
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    • pp.103-108
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    • 2005
  • To study the direct effect of somatostatin (SS) on calcium channel current ($I_{Ba}$) in guinea-pig gastric myocytes, $I_{Ba}$ was recorded by using whole-cell patch clamp technique in single smooth muscle cells. Nicardipine ($1{\mu}M$), a L-type $Ca^{2+}$ channel blocker, inhibited $I_{Ba}$ by $98{\pm}1.9$% (n=5), however $I_{Ba}$ was decreased in a reversible manner by application of SS. The peak $I_{Ba}$ at 0 mV were decreased to $95{\pm}1.5$, $92{\pm}1.9$, $82{\pm}4.0$, $66{\pm}5.8$, $10{\pm}2.9$% at $10^{-10}$, $10^{-9}$, $10^{-8}$, $10^{-7}$, $10^{-5}$ M of SS, respectively (n=3∼6; $mean{\pm}SEM$). The steady-state activation and inactivation curves of $I_{Ba}$ as a function of membrane potentials were well fitted by a Boltzmann equation. Voltage of half-activation ($V_{0.5}$) was $-12{\pm}0.5$ mV in control and $-11{\pm}1.9$ mV in SS treated groups (respectively, n=5). The same values of half-inactivation were $-35{\pm}1.4$ mV and $-35{\pm}1.9$ mV (respectively, n=5). There was no significant difference in activation and inactivation kinetics of $I_{Ba}$ by SS. Inhibitory effect of SS on $I_{Ba}$ was significantly reduced by either dialysis of intracellular solution with $GDP_{\beta}S$, a non-hydrolysable G protein inhibitor, or pretreatment with pertussis toxin (PTX). SS also decreased contraction of guinea-pig gastric antral smooth muscle. In conclusion, SS decreases voltage-dependent L-type calcium channel current ($VDCC_L$) via PTXsensitive signaling pathways in guinea-pig antral circular myocytes.

Effects of Sahyangsohap-won on Cerebral Hemodynamics in Healthy Subjects (사향소합원(麝香蘇合元)이 정상인의 뇌혈류역학에 미치는 영향)

  • Koo, Bon-Soo;Kim, Sung-Hwan;Moon, Sang-Kwan;Cho, Ki-Ho;Kim, Young-Suk;Bae, Hyung-Sup;Lee, Kyung-Sup;Ryu, Soon-Hyun
    • The Journal of Internal Korean Medicine
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    • v.22 no.2
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    • pp.199-205
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    • 2001
  • Background and Purpose : Transcranial doppler ultrasonography(TCD) is a noninvasive and nonradioactive technique for evaluation of the hemodynamics in large cerebral vessels. Sahyangsohap-won(SS) has been considered to be effective for the treatment of various disease, especially cerebrovascular, cardiovascular, and psychosomatoform disorders. But, there is no study about the effect of SS on the cerebral hemodynamics in humans. The aim of this study was to assess the effect of SS on the changes in cerebral hemodynamics and the dose-dependant effect by using TCD. Subjects and Methods : 30 healthy subjects were randomly divided into three group: group 1 took no drug, group 2 took SS one pill, and group 3 took SS 2 pills. Changes in the mean blood flow velocity(MBFV) and pulsatility index(PI) in the middle cerebral artery were evaluated by means of TCD. We obtained hypercapnia with breath-holding and evaluated cerebrovascular reactivity with the breath-holding index(BHI). Systolic blood pressure(SBP), diastolic blood pressure(DBP), and heart rate(HR) were measured by means of ambulatory blood pressure monitoring. In group 2 and group 3, the evaluations were performed during the baseline and were repeated at 20, 40, and 60 minutes after SS administration. In group 1, the evaluation was performed at corresponding time intervals. Results : In mean values of MSFV, PI, SSP, DBP, and HR, no stastically significant differences were found between the 3 groups. However, BHI values were significantly lower in groups 2 and 3 than in group 1 at 40 minutes after SS administration(P<0.05, group 1 vs group 2, group 1 vs group 3 by post-hoc analysis: Scheffe's test) but in dose-dependant effect, there was no difference between group 2 and group 3. Conclusion : These results suggest that SS can decrease vascular resistance in cerebral small arteries or arterioles and enhance their distensibility. Further studies on larger numbers of subjects are needed to confirm these effects and the dose-dependant effects.

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A Study on the Fatigue Crack Growth Behavior in Residual Stress Field of the SS41 Welding Material (SS41 용접재의 잔류응력장내에서의 피로균열 성장거동에 관한 연구)

  • Choi, Byung-Ki
    • Journal of the Korean Society of Safety
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    • v.17 no.1
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    • pp.33-38
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    • 2002
  • SS41 material is welded automatically and is investigated some effects of the welding residual stress on the growth and propagation of fatigue crack, so as to study the fatigue behaviour in the welding residual stress field. The summarized results are as follows; 1) In case of the load amplitude is constant, as the stress ration is changing to 0.1, 0.33 and 0.5 the propagation life is constant but the initiation life decreases. And than, when maximum load or minimum load is constant, s the stress ration increases the initiation life and propagation life. 2) It was shown that the fatigue crack propagation ratio da/dn was almost constant regardless of the stress ratio change at constant load amplitude and that the larger stress ratio, the slower was the fatigue crack propagation ratio. 3) The opening ratio U is influenced by $K_{max}$ but it isn't only the function of $K_{max}$ because data range is very large. 4) The fatigue life of the specimens on tensile compressive residual stress field was decreased and increased respectably more than that of the base metal.

Investigating the Causes and Control Measures for Precipitated Suspended Solids in the Underground Reservoir Tank in an Apartment (공동주택 지하저수조 내 침전된 부유성 고형물의 발생원인 및 제어방안)

  • JunYoung Jang;JooWon Kim;KiPal Kim;HyunSang Shin;ByungRan Lim
    • Journal of Korean Society on Water Environment
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    • v.39 no.2
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    • pp.153-161
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    • 2023
  • The reservoir tank in an apartment is crucial for maintaining the quality of drinking water after it has undergone treatment. Investigating the water quality and potential contaminants in the reservoir tank is essential to ensure the safety of the drinking water. This study examined the water quality and precipitated suspended solids that accumulate at the bottom of the reservoir tanks in four apartments located in Gyeonggi province. As a result of the water quality investigation, turbidity increased proportionally to the distance from the water treatment plant (WTP) to the household. Heavy metals were also detected in the reservoir tank inlet but not in the water supplied from the WTP. The precipitated suspended solids (SS) in the reservoir tank contain high levels of heavy metals and total organic carbon (TOC). The precipitated SS mainly consists of Al, Mn, and Fe, which are expected to be a combination with turbidity-inducing substances. The X-ray diffraction (XRD) analysis revealed the presence of γ-FeO(OH), MnO2, and β-Fe2O3 in the SS. Additionally, F-EEM analysis indicates that the dissolved organic matter in the SS is mainly derived from a natural water source and microorganism activities, including metal-oxidizing bacteria and biofilms that can absorb metal ions. Based on these findings, several countermeasures can be taken to prevent the inflow of SS into the household, including regularly cleaning the reservoir tank, replacing or cleaning old pipes in the water supply system, and implementing monitoring and filtering systems to manage the SS.

Numerical Simulations for Dispersion of the Suspended Sediments Near Daesan Coastal Areas (대산항 해역의 부유사 확산 수치모사)

  • Kim, Jin-Hyuk;Park, Gun-Hyung;Kim, Ki-Chul;Suh, Kyung-Suk
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.1
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    • pp.16-24
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    • 2011
  • EFDC model was applied to reproduce velocity fields and to evaluate the dispersion characteristics of suspended sediments (SS) around a Daesan port. Numerical results using two-dimensional hydrodynamic model of EFDC showed good agreements through comparison with the time series and harmonic analysis of the tidal elevations. The dispersion patterns of the suspended sediments using the calculated velocity fields were calculated to move from east to northeast direction in flood tide and from west to southwest in ebb tide for dredging of sea route, respectively. Also, the suspended sediments were widely dispersed into the front areas of a Daesan port, Nanji-do and Garorim bay in the long-term. Therefore, it was inferred that the environmental problems for sea pollution would be occurred seriously if the dredging for sea route would be continued in the long-term.