• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.471-477
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• 1998

Taq DNA polymerase from Thermus aquaticus is very useful in the polymerase chain reaction. Taq DNA polymerase is classified in the pol I family, represented by E. coli DNA polymerase I. The three-dimensional structural alignment of 3'-5'exonuclease domains from the pol I family DNA polymerases explains why Taq DNA polymerase does not carry out proofreading in polymerase chain reactions. Three sequence motifs, Exo I, II, and III, must exist to carry out 3'-5'exonuclease activity for proof- reading by a 3'-5'exonuclease reaction, but these are abolished in Taq DNA polymerase. The key catalytic module in 3'-5'exonuclease is two metal ions chelated by four active-site carboxylic amino acids. Taq DNA polymerase was mutagenized to construct the catalytic module in the active site. The circular dichroism technique supported the formation of the catalytic module, and the radioactive assay showed that the 3'-5'exonuclease activity doubled in the mutant Taq DNA polymerase.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.834-839
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• 2001

Calandria tube wrapping each pressure tube is one of the key structural components of CANDU reactor(Calandria) which is consisted of many pressure tubes containing nuclear fuel assemblies. As the Calandria tube(made of zirconium alloy) is sagging due to its thermal and irradiation creep during the plant operation, it possibly contacts with liquid injection nozzle crossing beneath the Calandria tube, which subsequently results in difficulties on the safe operation. It is therefore necessary to check the gap for the confirmation of no contacts between the two tubes, Calandria tube and liquid injection tube, with a proper measure during the life of plant. In this study, an ultrasonic measurement method was selected among several methods investigated. The ultrasonic device being developed for the measurement of the gap was introduced and its preliminary performance test results were presented here. The gap between LIN and CT at site was measured using by this ultrasonic device at site.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.265-273
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• 2011

Bacterial diversity based on the denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA gene sequences was determined for soil samples from two abandoned mine sites and the corresponding enrichment cultures using soil sample as key inoculum. Sequencing analysis of DGGE bands obtained from both the soil samples matched mostly with sequences of uncultured and newly described organisms, or organisms recently associated with the acid mine drainage environment. However, the enrichment of soil samples in ferrous sulfate and elemental sulfur media yielded sequences that were consistent with well-known iron- and sulfur-oxidizing acidophilic bacteria. Analysis of enrichment cultures of soil samples from Dalsung mine revealed abundant ${\gamma}$-$Proteobacteria$, whereas that of Gubong mine sample displayed acidophilic groups of ${\gamma}$-$Proteobacteria$, ${\alpha}$-$Proteobacteria$, $Actinobacteria$ and $Firmicutes$. Chemical elemental analysis of the mine samples indicated that the Dalsung site contained more iron and sulfate along with other toxic components as compared with those of the Gubong site. Biogeochemistry was believed to be the primary control on the acidophilic bacterial group in the enrichment samples.

• Journal of the Korea Computer Industry Society
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• v.4 no.10
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• pp.621-630
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• 2003

Web logs are the information recorded by a web server when users access the web sites, and due to a speedy rising of internet usage, the worth of their practical use has become increasingly important. Analyzing such logs can use to determine the patterns representing users' navigational behavior in a Web site and restructure a Web site to create a more effective organizational presence. For these applications, the generally used key methods in many studies are association rules and sequential patterns based by Apriori algorithms, which are widely used to extract correlation among patterns. But Apriori inhere inefficiency in computing cost when applied to large databases. In this paper, we develop a new algorithm for mining interesting patterns which is faster than Apriori algorithm and recommendation agents which could provide a system manager with valuable information that are accessed sequentially by many users.

• Proceedings of the Korea Society of Information Technology Applications Conference
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• 2007.05a
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• pp.321-349
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• 2007

The objective of this study is to investigate factors influencing trust on internet shopping mall and to examine whether these factors on trust have differences between Korean and Chinese users. Based on the literature reviews, this study employs six factors in two groups of influencing factors, web-site characteristics and individual characteristics as key determinants of trust in internet shopping mall. Analysis of 470 responses (Korean: 320 users, Chinese: 150 users) of survey questionnaire indicates the following: In terms of the relationship between web-site characteristics factors and the trust, the results showed clear differences between Korean and Chinese samples. For the Korean samples, transaction security and interactivity were the only significant influencing factors. For the Chinese samples, perceived reputation and perceived size were the only significant factors. In terms of individual characteristics factors, the influencing factors were found identical for the samples. Both propensity to trust and familiarity were significant factors in both Korean and Chinese samples. Based on the findings, managerial implications are discussed in building user trust in internet shopping mall in Korean and Chinese market.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.2
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• pp.127-132
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• 2002

All the countries in RA II (Asia Region in WMO) welcome the establishment of a Web site dedicated to agricultural meteorology, because it is believed that the best way to improve and speed up the flow of information is the use of the Internet and the establishment of a Web site. In providing recommendation for the promotion and improvement of the AgroMeteorological service in RA II, a couple of key suggestions were proposed: (a) Exchanges of data and AgroMeteorological knowledge between member countries and between RAs, (b) Exchanges of experts between member countries as a necessary way to share the knowledge, and (c) Joint research between member countries to solve common problems in AgroMeteorological affairs. In order to meet the above requirements for RA II, an AgroMeteorological information network will be the most critical and dynamic aspect in sustainable agriculture in this region. In addition, the establishment of a Core AgroMeteorological station, recommended by CAgM of WMO, will require its own information sharing systems for communication among member countries. Inevitable use of information technologies (IT) such as information networks, databases, simulation models, GIS, and RS for regional impact assessment of environmental change on AgroEcosystem will be enforced. Thus, the regional Internet-based Agrometeorological information network has been in place since 1999, though all contributions to it have been volunteered by individuals, institutes, universities, etc.

• Korean Institute of Interior Design Journal
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• v.20 no.3
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• pp.59-68
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• 2011

As society alters and building ages, many industrial facilities lost their function and abandoned in central city areas, and sometimes creating many problems. However, many European countries successfully transformed those urban decay to vivid cultural hub. The purpose of this study was to analyze a concept and methods of converting idle industrial facilities to successful cultural spaces. A case study of Tate Modern, Baltic Center for Contemporary Art, and Ruhr Museum was conducted using literature review, site visit, and interview methods. Findings indicated that converting historically significant idle industrial facilities to cultural center had not only reused abandoned site but also helped regenerating adjacent urban areas. This article demonstrates the key factors of successful conversion strategies as convenience, participation, placeness, historicity, and accessibly; and thus an effort to actively enhance the strategic factors were demanded in future conversion projects. A continuous studies on exploring extensive cases in various perspectives are required for further generalization in future studies.

• Korean Management Science Review
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• v.21 no.1
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• pp.1-17
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• 2004

A separate and distinct interaction with both the actual e-vendor and with its IT web site interface is at the heart of online shopping. Previous research has established, accordingly, that online purchase intention is the product of customer assessments of IT itself, specifically its perceived usefulness and ease-of-use(TAM). Since perceived usefulness and perceived ease of use are such a fundamental driver of usage intentions, it is important to understand the determinants of this construct and how their influence changes over the voluntariness towards the usage intention. The reason for voluntariness as a moderating variable is that it had the serious effect to the motivating in the making willingness of the internet shopping-mall usage. A better understanding of the determinants of perceived usefulness and perceived ease of use to the usage intention would enable us to design web site interventions that would increase user acceptance and usage of internet shopping-mall. Therefore, the goal of the present research is to extend TAM to include additional key determinants of TAM and to understand how the effects of these determinants change with the voluntariness of the usage intention of the internet shopping-mall.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1760-1772
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• 2006

Mature acetylcholinesterase (AChE) gene (gm, 1,836 bp) was cloned from the housefly and successfully expressed in the E. coli CodonPlus (DE3) RIL system (GM-E, 72 kDa) with a yield of 1,630 mU/g fresh cells. Using the gm, 10 kinds of mutants were constructed and expressed for enhancing sensitivity to insecticides. The sensitivity of these mutants to five kinds of organophosphate (OP) and three carbamate insecticides was investigated by measuring the apparent bimolecular inhibition constant ($k_i=k_2/K_d$). Surprisingly, the sensitivity of quadruple mutant IGFT was enhanced as much as 7-fold for acephate, 164-fold for demeton-S-methyl, 484-fold for dichlorvos, 523-fold for edifenphos, 30-fold for ethoprophos, 30-fold for benfuracarb, 404-fold for carbaryl, and 107-fold for furathiocarb, compared with that of GM-E, although the sensitivity of each single point mutant was slightly increased. These mutational studies indicated that (i) contradictory to Walsh et al. [39], the residue 327 is the important key residue for enhancing sensitivity as much as the residue 262, (ii) the residue 82 and additional residues of 234, 236, and 585 are also important, and (iii) sensitivity was cooperatively accelerated as the number of strategic mutations increased.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.1
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• pp.4-14
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• 2005

CarboKorea and HydroKorea are the domestic projects aiming to improve our understanding of carbon and water cycles in a typical Korean forest located in a complex terrain with a watershed connected to large rivers. The ultimate goal is to provide a nowcasting of these cycles for the whole Peninsula. The basic strategy to achieve such goal is through the inter- and multi-disciplinary studies that synthesize the in-situ field observation, modeling and remote sensing technology. The challenge is the fact that natural ecosystems are nonlinear and heterogeneous with a wide range of spatio-temporal scales causing the variations of mass and energy exchanges from a leaf to landscape scales. Our paradigm now shifts from temporal variation at a point to spatial patterns and from spatial homogeneity to complexity of water and carbon at multiple scales. Yet, a large portion of our knowledge about land-atmosphere interactions has been established based on tower observations, indicating that the development of scaling logics holds the key to the success of CarboKorea and HydroKorea. Here, we review the pioneering work of FIFE (First ISLSCP Field Experiment) on scaling issues in a temperate grassland and discuss the lessons from it for the application to Gwangneung forest site.