• Wind and Structures
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• v.17 no.1
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• pp.43-68
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• 2013

The fluctuating wind induced vibration is one of the most important factors which has been taken into account in the design of long-span bridge due to the low stiffness and low natural frequency. Field measurement characteristics of sustained wind on structure site can provide accurate wind load parameters for wind field simulation and structural wind resistance design. As a suspension bridge with 1490 m main span, the Runyang Suspension Bridge (RSB) has high sensitivity to fluctuating wind. The simultaneous and continuously wind environment field measurement both in mid-span and on tower top is executed from 2005 up to now by the structural health monitoring system installed on this bridge. Based on the recorded data, the wind characteristic parameters, including mean wind speed, wind direction, the turbulence intensity, the gust factors, the turbulence integral length, power spectrum and spatial correlation, are analyzed in detail and the coherence functions of those parameters are evaluated using statistical method in this paper. The results indicate that, the turbulence component of sustain wind is larger than extremely strong winds although its mean wind speed is smaller; the correlation between turbulence parameters is obvious; the power spectrum is special and not accord with the Simiu spectrum and von Karman spectrum. Results obtained in this study can be used to evaluate the long term reliability of the Runyang Suspension Bridge and provide reference values for wind resistant design of other structures in this region.

• Journal of Electrical Engineering and Technology
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• v.9 no.4
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• pp.1385-1393
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• 2014

Gas insulated switchgear (GIS) is widely used in the power systems, however, the contacts overheating of the inside circuit breaker or disconnector may be a potential cause of developing accidents. As the temperature of the contacts cannot be directly acquired due to existence of the metallic shield, an infrared sensor is adopted to directly measure the temperature of the shield and then the contacts temperature can be indirectly obtained by data fitting, based on which the on-line temperature monitoring technology specifically for GIS contacts based on infrared sensing is proposed in this paper. A real GIS test platform is constructed and experimental studies are carried out to account for the influential factors that affect the accuracy of the infrared temperature measurement. A heat transfer model of the GIS module is also developed, together with experimental studies, the nonlinear temperature relationship among the contacts, the metallic shield and the environment based on a neural network algorithm is established. Finally, an integrated on-line temperature monitoring system for the GIS contacts is developed for on-site applications.

• Geomechanics and Engineering
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• v.13 no.5
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• pp.861-879
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• 2017

Coal and gas outburst is a serious dynamic disaster that occurs during coal mining and threatens the lives of coal miners. Currently, coal and gas outburst is commonly predicted using single indicator and its critical value. However, single indicator is unable to fully reflect all of the factors impacting outburst risk and has poor prediction accuracy. Therefore, a more accurate prediction method is necessary. In this work, we first analyzed on-site impacting factors and precursors of coal and gas outburst; then, we constructed a Fisher discriminant analysis (FDA) index system using the gas adsorption index of drilling cutting ${\Delta}h_2$, the drilling cutting weight S, the initial velocity of gas emission from borehole q, the thickness of soft coal h, and the maximum ratio of post-blasting gas emission peak to pre-blasting gas emission $B_{max}$; finally, we studied an FDA-based multiple indicators discriminant model of coal and gas outburst, and applied the discriminant model to predict coal and gas outburst. The results showed that the discriminant model has 100% prediction accuracy, even when some conventional indexes are lower than the warning criteria. The FDA method has a broad application prospects in coal and gas outburst prediction.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.167-179
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• 2023

The rifampicin-resistant strain E9-302 of Edwardsiella ictaluri strain 669 (WT) was generated by continuous passage on BHI agar plates containing increasing concentrations of rifampicin. E9-302 was attenuated significantly by 119 times to zebrafish Danio rerio compared to WT in terms of the 50% lethal dose (LD₅₀). Zebrafish vaccinated with E9-302 via intraperitoneal (IP) injection at a dose of 1 × 10³ CFU/fish had relative percentage survival (RPS) rates of 85.7% when challenged with wild-type E. ictaluri via IP 14 days post-vaccination (dpv). After 14 days of primary vaccination with E9-302 via immersion (IM) at a dose of 4 × 10⁷ CFU/ml, a booster IM vaccination with E9-302 at a dose of 2 × 10⁷ CFU/ml exhibited 65.2% RPS against challenge with wild-type E. ictaluri via IP 7 days later. These results indicated that the rifampicin-resistant attenuated strain E9-302 had potential as a live vaccine against E. ictaluri infection. A previously unreported amino acid site change at position 142 of the RNA polymerase (RNAP) β subunit encoded by the gene rpoB associated with rifampicin resistance was identified. Analysis of the whole-genome sequencing results revealed multiple missense mutations in the virulence-related genes esrB and sspH2 in E9-302 compared with WT, and a 189 bp mismatch in one gene, whose coding product was highly homologous to glycosyltransferase family 39 protein. This study preliminarily explored the molecular mechanism underlying the virulence attenuation of rifampicin-resistant strain E9-302 and provided a new target for the subsequent study of the pathogenic mechanism of E. ictaluri.

• International conference on construction engineering and project management
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• 2020.12a
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• pp.343-352
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• 2020

Modular integrated construction (MiC) is an innovative construction method where components of a building are manufactured in an offsite factory, trucked to the job site in sections, set in place with cranes, and assembled together to form a whole building. Where circumstances merit, favorable conditions exist and implemented effectively; MiC improves project performance. However, several key factors need to converge during implementation to realize the full benefits of MiC. Thus, a thorough understanding of the factors which are critical to the success of MiC projects is imperative. Drawing on a systematic review of 47 empirical studies, this research identified 25 key success factors (KSFs) for MiC projects. Of these, the five topmost cited KSFs for MiC projects include effective working collaboration and communication among project participants; standardization, optimization, automation and benchmarking of best practices; effective supply chain management; early design freeze and completion; and efficient procurement method and contracting. The study further proposed a conceptual model of the KSFs, highlighting the interdependences of people, processes, and technology-related KSFs for the effective accomplishment of MiC projects. The set of KSFs is practically relevant as they constitute a checklist of items for management to address and deal with during the planning and execution of MiC projects. They also provide a useful basis for future empirical studies tailored towards measuring the performance and success of MiC projects. MiC project participants and stakeholders will find this research useful in reducing failure risks and achieving more desired performance outcomes. One potential impact of the study is that it may inform, guide, and improve the successful implementation of MiC projects in the construction industry. However, the rigor of the analysis and relative importance ranking of the KSFs were limited due to the absence of data.

• Journal of Korean Society of Forest Science
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• v.90 no.6
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• pp.774-780
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• 2001

Aboveground carbon storage and increment of a 31-year-old pitch pine (Pinus rigida) stand were measured for five years (1997~2001) in the Jungbu Forest Experiment Station, Gyeonggi-do, Korea. The carbon concentration in each component of aboveground and soil depth decreased in the order of needle>branch>stembark>stemwood>forest floor>0-15cm soil depth>15-30cm soil depth. The carbon storage except for root carbon was 140,600kgC/ha and the tree accounted for 61%, soil 31% and forest floor 8% of the stand carbon storage. Due to high tree mortality by Fusarium subglutinans infection and spring drought in 2001, carbon increment except for 2001 data was 3,233kgC/ha/yr and was in the order of stemwood>branch>stembark>needle. Carbon storage and increment were attributed to stand density and site quality. Carbon storage and increment were higher in the high site quality than in the lower site quality plot on similar tree density. Also, the high tree density site on similar site quality showed more carbon storage and increment compared with the lower tree density. The results suggest that site quality and tree density are a key factor determining carbon storage and increment in this pitch pine stand.

• Korean Journal of Construction Engineering and Management
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• v.22 no.2
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• pp.21-30
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• 2021

The existing construction industry is classified into industries with low productivity compared to other industries. In order to solve the decline in productivity in the construction industry, the world is focusing on off-site construction (OSC), which is about 40% more productive than existing construction methods. This high productivity is possible because the three stages of production, transport to the site, and site assembly are consistently maintained in a continuous flow. This study conducted a functional survey through literature review, preliminary field survey, questionnaire, and expert interview. The surveyed function were classified into five categories: convenience, flexibility, manageability, communication, and safety, and the functional characteristics of flexibility, manageability, and communication were high. Because of the change according to the construction progress, the production schedule of the factory, and the variety of transportation time required. Finally, A functional block diagram was developed based on the 15 functions corresponding with an average score of 4 or more in the surveyed function score result. If the OSC transport system is established through this study, It can contribute to a successful construction OSC project and increase productivity.

• Journal of Ecology and Environment
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• v.47 no.4
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• pp.228-240
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• 2023

Growing complexity in ecosystem structure and functions, under impacts of climate and land-use changes, requires interdisciplinary understandings of processes and the whole-system, and accurate estimates of the changing functions. In the last three decades, observation networks for biodiversity, ecosystems, and ecosystem functions under climate change, have been developed by interested scientists, research institutions and universities. In this paper we will review (1) the development and on-going activities of those observation networks, (2) some outcomes from forest carbon cycle studies at our super-site "Takayama site" in Japan, and (3) a few ideas how we connect in-situ and satellite observations as well as fill observation gaps in the Asia-Oceania region. There have been many intensive research and networking efforts to promote investigations for ecosystem change and functions (e.g., Long-Term Ecological Research Network), measurements of greenhouse gas, heat, and water fluxes (flux network), and biodiversity from genetic to ecosystem level (Biodiversity Observation Network). Combining those in-situ field research data with modeling analysis and satellite remote sensing allows the research communities to up-scale spatially from local to global, and temporally from the past to future. These observation networks oftern use different methodologies and target different scientific disciplines. However growing needs for comprehensive observations to understand the response of biodiversity and ecosystem functions to climate and societal changes at local, national, regional, and global scales are providing opportunities and expectations to network these networks. Among the challenges to produce and share integrated knowledge on climate, ecosystem functions and biodiversity, filling scale-gaps in space and time among the phenomena is crucial. To showcase such efforts, interdisciplinary research at 'Takayama super-site' was reviewed by focusing on studies on forest carbon cycle and phenology. A key approach to respond to multidisciplinary questions is to integrate in-situ field research, ecosystem modeling, and satellite remote sensing by developing cross-scale methodologies at long-term observation field sites called "super-sites". The research approach at 'Takayama site' in Japan showcases this response to the needs of multidisciplinary questions and further development of terrestrial ecosystem research to address environmental change issues from local to national, regional and global scales.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.9
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• pp.1256-1266
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• 2009

Effects of particle size and physical effective fibre (peNDF) of rice straw in diets on chewing activities, feed intake, flow, site and extent of digestion and rumen fermentation in goats were investigated. A 4${\times}$4 Latin square design was employed using 4 mature Liuyang black goats fitted with permanent ruminal, duodenal, and terminal ileal fistulae. During each of the 4 periods, goats were offered 1 of 4 diets that were similar in nutritional content but varied in particle sizes and peNDF through alteration of the theoretical cut length of rice straw (10, 20, 40, and 80 mm, respectively). Dietary peNDF contents were determined using a sieve for particle separation above 8 mm, and were 17.4, 20.9, 22.5 and 25.4%, respectively. Results showed that increasing the particle size and peNDF significantly (p<0.05) increased the time spent on rumination and chewing activities, duodenal starch digestibility and ruminal pH, and decreased ruminal starch digestibility and $NH_{3}$-N concentration. Intake and total tract digestibility of nutrients (i.e. dry matter, organic matter, and starch) and ruminal fermentation were not affected by the dietary particle size and peNDF. Increased particle size and peNDF did not affect ruminal fibre digestibility, but had a great impact on the intestinal and total tract fibre digestibility. The study suggested that rice straw particle size or dietary peNDF was the important influential factor for chewing activity, intestinal fibre and starch digestibility, and ruminal pH, but had minimal impact on feed intake, duodenal and ileal flow, ruminal and total tract digestibility, and ruminal fermentation.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.324-328
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• 2006

Carbonic anhydrase III (CA3) is a member of a multigene family that encode carbonic anhydrase isozymes. In this study, a complete coding sequence of the pig CA3 gene which encodes a 260 amino-acid protein was determined. The amino acid comparison showed high sequence similarities with previously identified human (86.5%) CA3 gene and mouse (91.5%) Car3 gene. The partial genomic DNA sequences were also investigated. The length of intron 1 was 727 bp. Comparative sequencing of three pig breeds revealed that there was a T${\rightarrow}$C substitution at position 363 within intron 1. The substitution was situated within a NcoI recognition site and was developed as a PCR-restriction fragment length polymorphism (RFLP) marker for further use in population variation investigations and association analysis. Two alleles (A and B) were identified, and 617 bp fragments were observed for the AA genotype and 236 bp and 381 bp fragments for the BB genotype. The polymorphism of CA3 was detected in 8 pig breeds. Allele B was predominant in the Western pig breeds. In addition, association studies of the CA3 polymorphism with carcass traits in 140 $Yorkshire{\times}Meishan$ $F_2$ offspring showed that the NcoI PCR- RFLP genotype may be associated with variation in several carcass traits of interest for pig breeding. Allele B was associated with increases in lean meat percentage, loin eye height and loin eye area. Statistically significant association with backfat thickness was also found; pigs with the AB genotype had much less backfat thickness than AA or BB genotypes.