• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.401-408
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• 2005

Recently shield TBM tunnellings are being applied to subway construction in Korean cities. Generally these kinds of tunnellings have the problems in the stability of ground such as subsidence because urban subway is constructed in the shallow depth. A sinkhole occurred on the road just above the tunnel during tunneling in Kwangju, so a survey for upper layer of the tunnel was needed. But conventional Ground Probing Radar can't be applicable due to the presence of steel-mesh screen in the shield segment, so no existent geophysical method is applicable in this site. Because the outer surface of each shield segment is electrically insulated, dipole-dipole resistivity method which is popular in engineering site investigation, was tried to this survey for the first time. Specially manufactured flexible ring-type electrodes were installed into the grouting holes at an interval of 2.4 m on the ceiling. The K-Ohm II system which has been developed by KIGAM and tested successfully in many sites, was used in this site. The system consists of 1000Volt-1Ampere constant-current transmitter, optically isolated 24 bit sigma-delta A/D conversion receiver - maximum 12 channel simultaneous measurements, and graphical automatic acquisition software for easy data quality check in real time. Borehole camera logging with circular white LED lighting was also done to investigate the state of the layer. Measured resistivity data lack of some stations due to failing opening lids of holes, shows general high-low trend well. The dipole-dipole resistivity inversion results discriminate (1) one approximately 4 meter diameter cavity (grouted but incompletely hardened, so low resistivity - less than $30{\Omega}m$), (2) weak zone (100-200${\Omega}m$), and (3) hard zone (high resistivity - more than 1000${\Omega}m$) very well for the distance of 320 meters. The 2-D inversion neglects slight absolute 3-D effect, but we can get satisfactory and useful information. Acquired resistivity section and video tapes by borehole camera logging will be reserved and reused if some problem occurs in this site in the future.

• Archives of Pharmacal Research
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• v.28 no.8
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• pp.956-962
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• 2005

Leishmania virus (LRV)1-4 has been reported to produce a fusion of ORF2 and ORF3 via a programmed +1 frameshift in the region where ORF2 and ORF3 overlap (Lee et a/., 1996). However, the exact frameshift site has not been identified. In this study, we compared the frameshift efficiency of a 259bp (nt. 2565-2823), frameshift region of LRV1-4, and the 71 bp (nt. 2605-2678) sub-region where ORF2 and ORF3 overlap. We then predicted the frameshift site using a new computer program (Pseudoviewer), and finally identified the specific region associated with the mechanism of the LRV1-4's+1 frameshift by means of a mutational analysis based on the predicted structure of LRV1-4 RNA. The predicted structure was confirmed by biochemical analysis. In order to measure the frameshift efficiency, constructs that generate luciferase without a frameshift or with a+1 frameshift, were generated and in vitro transcription/translation analysis was performed. Measurements of the luciferase activity generated, showed that the frameshift efficiency was about $1\%$ for both the 259bp (LRV1-4 259FS) and 71 bp region (LRV1-4 71FS). Luciferase activity was strongly reduced in a mutant (LRV1-4 NH: nt. 2635-2670) with the entire hairpin deleted and in a mutant (LRV1-4 NUS: nt. 2644-2659) with the upper stem of the hairpin deleted. These results indicate that the frameshift site in LRV1-4's is in the 71 bp region where ORF2 and ORF3 overlap, and that nt. 2644-2659 (the upward hairpin stem) playa key role in generating the +1 frameshift.

• Asian Journal of Turfgrass Science
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• v.10 no.3
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• pp.213-230
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• 1996

A study of occurrence and distribution of cellular slime molds(CSMs) in forest soils of central areas of Korea was carried out. Samples for CSMs isolation were collected from 4 study sites; Mt.Kyeryong, Mt. Taebaek, Mt. Sobaek and Mt. Sokri. In Mt. Kyeryong, six species were found. These were Dictyostelium fasciculatum, D.firmibasis, D. mucoroides, D. minutum, D. brefeldianum and Polysphondylium pallidum. The average number of species isolated at one site was 0.75, and average density(clones /g soil) was 292. The results of soil sample analyses were that the concentration of Pb was higher than any other areas. In Mt. Taebaek, seven species were found; D. fasciculatum, D.firmibasis, D. mucoroides, D. miuutum, P.pallidum. P. violaceum, P. candidum. The average number of species isolated at one site was 2.3,and average density was 1,108. Based on importance values calculated from study sites within each of three elevation ranges, several of the more widely distributed and abundant species have distribution patterns that show a response to elevation. In Mt. Sobaek, eleven species were found. These were D. fasciculatum. D.firmibasis, D. mucoroides, D. miuutum, D. brefeldianum, D. crassicaule,D. deminutivum, D. implicatum, P. pallidum. P. violaceum and P. candidum. The average number of species isolated at one site was 3, and average density was 793. Species diversity appeared to be the highest in this area. In Mt. Sokri, six species were found. These were D. fasciculatum. D. mucoroides, D. minutum, D. purpureum, P. pallidum. and P. violaceum. The average number of species isolated at one site was 2.4 and average density was 858. It was noticeable that D.purpureum were much more comrnonly found in this pinus evergreen forest. Key words: Cellular slime molds(CSMs), Importance value, Elevation, Average number of species, Average density.

• Journal of Digital Convergence
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• v.15 no.1
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• pp.117-127
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• 2017

Even well trained personnel may fall victim to panic, and a wrong assessment of the situation can turn a small accident into a huge disaster. At the disaster site, the event is commonly broadcasted in real time and is unfiltered. Thus, the commander of the site must be able to accurately assess the scene while not missing out on key considerations. Despite the necessity of good situational assessment, there is barely any research on this topic. This study utilizes METT-TC(Mission, Enemy, Troops, Terrain and weather, Time available, and Civil considerations) which is used by militaries to assess combat situations, and proposes MORT-TAC(Mission, Object, Resources available, Terrain and weather, Time available, Ability, and Civil considerations) as factors to consider in effectively controlling the disaster site. The standard model is used for assessing disaster scenes by applying the Delphi analytical method on each sub-factor of MORT-TAC.

• Nutrition Research and Practice
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• v.2 no.2
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• pp.114-120
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• 2008

A diet habit, which is developed in childhood, lasts for a life time. In this sense, nutrition education and early exposure to healthy menus in childhood is important. Children these days have easy access to the internet. Thus, a web-based nutrition education program for children is an effective tool for nutrition education of children. This site provides the material of the nutrition education for children with characters which are personified nutrients. The 151 menus are stored in the site together with video script of the cooking process. The menus are classified by the criteria based on age, menu type and the ethnic origin of the menu. The site provides a search function. There are three kinds of search conditions which are key words, menu type and "between" expression of nutrients such as calorie and other nutrients. The site is developed with the operating system Windows 2003 Server, the web server ZEUS 5, development language JSP, and database management system Oracle 10 g.

• Journal of architectural history
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• v.30 no.3
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• pp.55-66
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• 2021

The purpose of this study is to review the research on Gangbang Palace and to presume the location of the palace through the study of feng shui. when the Mongols invaded, Ganghwado was the capital, moved from Gaegyeong and it was maintained for 39 years. At that time, the palace construction followed the palace of Gaegyeong, and the names such as Gujeong毬庭, Palace, and Sasa寺社 were the same as those of Gaegyeong. However, despite several archaeological excavations at Goryeo Palace Site, Historic Site No. 133, no actual remains of the Goryeo period were found. After the negative opinions on the current Goryeo Palace site became public, there have been several previous studies on the estimation of the location of the Goryeo Palace Site, and although there have been achievements, the location of the Goryeo Palace is still not confirmed. The key to presuming the location of the GangdoPalace江都宮闕 is first, whether the palace was located in the south of Songaksan, and second, at what point it was located in the south of Songaksan. As to whether the palace was located in the south of Songaksan, it was presumed that the Chiso治所 of Ganghwabu was located in Gukhwari, referring to the record in 『Shinjeungdongguknyeojiseungnam新增東國輿地勝覽』 that Goryeosan, a guardian mountain鎭山, was 'five ri west of Bu府西五里'. Accordingly, the location of the palace of 'Budongsimni 府東十里' in the old literature was estimated to be south of Songaksan Mountain. Also, the location of the palace on the south side of Songaksan was approached from a feng shui theory. Three places for the palace to be located are presumed to be 'Ganghwa Girls' High School', 'Seonggwang Church', or 'Above the Ganghwa-gun library', and the central axis of the palace is estimated to be 'Above the Ganghwa-gun library' where the corridor-type building relics were found among them, and the other two areas were presumed to be other buildings within the palace.

• Advances in environmental research
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• v.9 no.4
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• pp.275-284
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• 2020

We provide the first study on the occurrence of microplastics in Dal lake, Kashmir, India. Microplastics act as catastrophe that trigger many environmental problems. The key origins of microplastics are larger plastics, which split into smaller plastics after UV light disintegration. There is relatively little work carried out on the existence of microplastics. The present work has been undertaken on the occurrence of microplastics at four pre-selected sites (surface water) in Dal lake, Kashmir. The samples were taken to the laboratory to dissolve organic matter by using H2O2 (6%). To speed up the organic digestion; the treated mixture was heated on a hot plate at 70℃. The mixture was then subjected to density separation. The supernatant obtained was observed under the microscope (10X) and measurements were taken. At site-I, the microplastics ranged from 2-3 mm, site-II 5-6 mm, site-III 3-4 mm, site-IV 4-5 mm in thickness, indicating the presence of microplastics in the lake. The presence of microplastics indicated that the lake has undergone an anthropogenic change over a period of time. Our research highlights the value of enhancing the quality of the drainage system and sewage disposal. This work can be helpful to recognize successful microplastic control management techniques and possible threats associated with the Dal lake. So far, no such data on the presence of microplastics in Kashmir lakes is available.

• Journal of Information Technology Services
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• v.20 no.5
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• pp.11-28
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• 2021

As untact travel has become a new trend in leisure culture due to the spread of COVID-19, car camping market is rapidly increasing. The sales of car camping-related goods increased by up to 600 percent, and the sales of SUV in Korea also increased by about four times. Despite the growth of the car camping market, there is a lack of research on the actual condition of the car camping market or research on the user's perspective. Therefore, in this study, a survey of actual camping users was conducted to derive factors that they consider important in camping, and through this, a car camping map was produced. As a result, two types of maps were produced: a map about the car camping site and convenience facilities closest to the car camping site in Gangwon-do, and a hash tag themed map based on keywords for each car camping site. We gathered data on portal sites and social media to obtain information related to camping sites and proceeded with analysis using text mining. In addition, we extracted keywords using network analysis techniques and selected key themes that represent them. This allows the user to choose a car camping site by selecting keywords that suit their taste. We hope that this research will help car camping researchers as a prior study and provide a foundation for leading a clean camping culture through clean camping campaign. Also, we hope that car camping users will be able to do quality trip.

• Earthquakes and Structures
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• v.25 no.1
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• pp.27-41
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• 2023

This study focused on nonlinear effective stress site response analysis using two coupled constitutive models, that is, the DM model (Dafalias and Manzari 2004), which incorporated a simple plasticity sand model accounting for fabric change effects, and the PMDY03 model (Khosravifar et al. 2018), that is, a 3D model for earthquake-induced liquefaction triggering and postliquefaction response. A detailed parametric study was conducted to validate the effectiveness of nonlinear site response analysis and porewater pressure (PWP) generation through a true coupled formulation for assessing the initiation of liquefaction at ground level. The coupled models demonstrated accurate prediction of liquefaction triggering, which was in line with established empirical liquefaction triggering relations in published databases. Several limitations were identified in the evaluation of liquefaction using the cyclic stress method, despite its widespread implementation for calculating liquefaction triggering. Variations in shear stiffness, represented by changes in shear wave velocity (Vs1), exerted the most significant influence on site response. The study further indicated that substantial differences in response spectra between nonlinear total stress and nonlinear effective stress analyses primarily occurred when liquefaction was triggered or on the verge of being triggered, as shown by excess PWP ratios approaching unity. These differences diminished when liquefaction occurred towards the later stages of intense shaking. The soil response was predominantly influenced by the higher stiffness values present prior to liquefaction. A key contribution of this study was to validate the criteria used to assess the triggering of level-ground liquefaction using true coupled effective-stress constitutive models, while also confirming the reliability of numerical approximations including the PDMY03 and DM models. These models effectively captured the principal characteristics of liquefaction observed in field tests and laboratory experiments.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1085-1091
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• 2009

A phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. Therefore, this study used a degenerate PCR and TAIL PCR to clone a phytase gene from Erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. The full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kDa with a 50% amino acid identity to the Klebsiella pneumoniae phytase. The phytase contained the active site RHGXRXP and HD sequence motifs that are typical of histidine acid phosphatases. The enzyme was expressed in Escherichia coli, purified, and displayed the following characteristics: a high catalytic activity at low temperatures (retaining over 24% activity at $5^{\circ}C$) and remarkably thermal lability (losing >96% activity after incubation at $60^{\circ}C$ for 2 min). The optimal phytase activity occurred at pH 5.5 and ${\sim}49^{\circ}C$, and the enzyme activity rapidly decreased above $40^{\circ}C$. When compared with mesophilic counterparts, the phytase not only exhibited a high activity at a low temperature, but also had a low $K_m$ and high $k_{cat}$. These temperature characteristics and kinetic parameters are consistent with low-temperature-active enzymes. To our knowledge, this would appear to be the first report of a low-temperature-active phytase and its heterogeneous expression.