• Title/Summary/Keyword: Key Target System

Search Result 348, Processing Time 0.029 seconds

MALDI-TOF MS System for the Identification of Microorganisms in Milk and Dairy Products (우유 및 유제품 중 미생물 동정을 위한 MALDI-TOFMS활용)

  • Kim, Hyoun Wook;Ham, Jun-Sang;Seol, Kuk-Hwan;Han, Sangha;Park, Beam Young;Oh, Mi-Hwa
    • Journal of Dairy Science and Biotechnology
    • /
    • v.30 no.2
    • /
    • pp.131-137
    • /
    • 2012
  • Rapid and reliable identification of microorganisms is a key for tracing the relationship between the target bacteria and related infectious diseases. Various identification methods such as classical phenotypic analysis, numerical taxonomic analysis, and DNA sequencing have been widely used to classify microorganisms in milk and dairy products. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) identifies targeted bacteria in milk and milk products. Several studies have demonstrated that MALDI-TOF MS identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. MALDI-TOF MS could provide accurate identification of bacteria in milk and milk products at the serotype or strain level and enable antibiotic resistance profiling within minutes.

  • PDF

Quantitative Analysis of Leuconostoc mesenteroides and Lactobacillus plantarum Populations by a Competitive Polymerase Chain Reaction

  • Koh, Young-Ho;Kim, Myoung-Dong;Han, Nam-Soo;Seo, Jin-Ho
    • Journal of Microbiology and Biotechnology
    • /
    • v.12 no.5
    • /
    • pp.801-806
    • /
    • 2002
  • A multiplex competitive polymerase chain reaction (PCR) method was developed for the rapid identification and quantification of Leuconostoc mesnteroides and Lactobacillus plantarum populations which are the key microorganisms in kimchi fermentation. The strain-specific primers were designed to selectively amplify the target genes encoding 165 rRNA of L. plantarum and dextransucrase of L. mesenteroides. There was a linear relationship between the band intensity of PCR products and the number of colony forming units of each model organism. The PCR quantification method was compared with a traditional plate-counting method f3r the enumeration of the two lactic acid bacteria in a mixed suspension culture and also applied to a real food system, namely, watery kimchi. The population dynamics of the two model organisms in the mixed culture were reliably predictable by the competitive PCR analysis.

Internet Banking Login with Multi-Factor Authentication

  • Boonkrong, Sirapat
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.11 no.1
    • /
    • pp.511-535
    • /
    • 2017
  • Internet banking is one of many services provided by financial institutions that have become very popular with an increasing trend. Due to the increased amount of usage of the service, Internet banking has become a target from adversaries. One of the points that are at risk of an attack is the login process. Therefore, it is necessary to have a security mechanism that can reduce this risk. This research designs and develops a multi-factor authentication protocol, starting from a registration system, which generates authentication factors, to an actual authentication mechanism. These factors can be categorised into two groups: short term and long term. For the authentication protocol, only three messages need to be exchanged between a client and a financial institution's server. Many cryptographic processes are incorporated into the protocol, such as symmetric and asymmetric cryptography, a symmetric key generation process, a method for generating and verifying digital signatures. All of the authentication messages have been proved and analysed by the logic of GNY and the criteria of OWASP-AT-009. Even though there are additional factors of authentication, users do not really feel any extra load on their part, as shown by the satisfactory survey.

Business Strategy of Fast Fashion -A Case Study of Zara- (패스트 패션의 비즈니스 전략 -자라의 사례 연구-)

  • Kim, Gihyung;Lee, Seunghee
    • Journal of the Korean Society of Clothing and Textiles
    • /
    • v.38 no.2
    • /
    • pp.175-190
    • /
    • 2014
  • This study analyzes the business strategy of fast fashion through Zara, a successful fashion brand from Inditex Spain. An in-depth case approach is adopted based on extensive secondary research that includes literature and press releases published in Korean as well as English. The findings of this research demonstrate a speedy and flexible process occurring in the supply chain of its fast fashion business and the cooperation between the company's headquarters and international subsidiaries. Zara's headquarters executes four representative strategies: competitive market research, an integrated organizational structure, small quantity batch production, and a specialized distribution system. Zara's international subsidiaries execute their own four representative strategies: differentiated international expansion, independent human resource management, small but fundamental IT, and maximization of store resources. These two core parts intimately work together to satisfy target customers all over the world by bringing competitive advantages to the fashion business and represent a key concept of Zara's business strategy. The main drawbacks of case studies are limited validity and representativeness restraining the potential for making generalizations. However this case is considered sufficient to provide valuable insight and improve the understanding of operation strategy in fast fashion.

HoneyThing: A New Honeypot Design for CPE Devices

  • Erdem, Omer;Pektas, Abdurrahman;Kara, Mehmet
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.12 no.9
    • /
    • pp.4512-4526
    • /
    • 2018
  • The Internet of Things (IoT) has become an emerging industry that is broadly used in many fields from industrial and agricultural manufacturing to home automation and hospitality industry. Because of the sheer number of connected devices transmitting valuable data, the IoT infrastructures have become a main target for cyber-criminals. One of the key challenges in protecting IoT devices is the lack of security measures by design. Although there are many hardware and software based security solutions (firewalls, honeypots, IPDS, anti-virus etc.) for information systems, most of these solutions cannot be applied to IoT devices because of the fact that IoT devices have limited computing resources (CPU, RAM,). In this paper, we propose a honeypot system called HoneyThing for modem/router devices (i.e. a kind of IoT device). HoneyThing emulates TR-069 protocol which is prevalent protocol used to remotely manage customer-premises equipment (CPE) devices, e.g. modems, routers. Honeything also serves an embedded web server simulating a few actual, critical vulnerabilities associated with the implementation of TR-069 protocol. To show effectiveness of the HoneyThing in capturing real world attacks, we have deployed it in the Internet. The obtained results are highly promising and facilitate to reveal network attacks targeting to CPE devices.

ADD's Test Facilities for National Defense Magnetic Research (국방 자기분야 연구를 위한 국방과학연구소 시험시설)

  • Yang, Chang-Seob;Chung, Hyun-Ju;Jung, Woo-Jin
    • Journal of the Korean Magnetics Society
    • /
    • v.24 no.4
    • /
    • pp.114-122
    • /
    • 2014
  • Since 1990, Agency for Defense Development (ADD) has been operating the nonmagnetic laboratory and the magnetic sensors laboratory to develop the key technology of the ship's magnetic silencing and to research the high performance magnetic sensors used in the weapon system and to perform the technical support for Republic of Korea Navy. Recently, the main test equipment in the laboratories has been upgraded to improve the measurement and analysis abilities against magnetic target and to evaluate the performance of the magnetic sensors. In this paper, the capability and the current state of magnetic test facilities of ADD are described.

Construction of Citrus Transgenic Plant with Fatty Aicd Desaturase Gene

  • Jin, Seong-Beom;Boo, Kyung-Hwan;Lee, Do-Seung;Chae, Hyun-Byung;Song, Seong-Jun;Riu, Key-Zung
    • Journal of Applied Biological Chemistry
    • /
    • v.42 no.3
    • /
    • pp.113-118
    • /
    • 1999
  • The transgenic plant of Citrus species (Citrus aurantium L.) was constructed with a fatty acid desaturase gene using microprojectile bombardment transformation system. The DNA of a fatty acid desaturase gene, fad7, constructed in pBI121 was coated onto tungsten particles ($1.1{\mu}m$) and introduced into callus cells by bombarding with 1100 psi of helium pressure, 1/4 in of gap distance, 7.0 cm of target distance and 27 in Hg of chamber vacuum. The bombarded cells were selected on the medium containing kanamycin. The selected cells were successfully regenerated into plantlets via somatic embryogenesis on the media containing plant growth regulators. The results of polymerase chain reaction analysis of genomic DNAs from the putative transformants showed that the introduced DNAs of fad7 were present in both the selected callus cells and the regenerated plantlets.

  • PDF

Experimental study on analysis of correlation between void fraction and drag reduction rate in air lubrication ship (공기윤활선 모사 실험에서의 공극률 및 마찰저항저감율 상관성 분석을 위한 실험적 연구)

  • Park, Seungchan;Lee, Inwon
    • Journal of the Korean Society of Visualization
    • /
    • v.18 no.1
    • /
    • pp.11-17
    • /
    • 2020
  • The reduction of CO2 emissions has been a key target in the marine industry since the IMO's MEPC published its findings in 2009. Air lubrication method is one of the mature technologies for commercialization to reduce the frictional resistance and enhance fuel efficiency of ships. Since the air lubrication pattern varies according to the ship's standing position and injection flow rate, in order to effectively control the air lubrication system, it is necessary to be able to judge the air layer development state based on the information collected from the monitoring sensor. In this study, we performed the air lubrication ship simulation experiment to measure the void fraction and the frictional resistance. The void fraction was measured to confirm the behavior of the air. Through the measurement of the frictional resistance, the change in frictional resistance reduction rate from the injection point to the longitudinal direction of the ship was confirmed. Based on the measurement results, correlation analysis was performed on void fraction and frictional resistance reduction rate.

Interaction Analysis of Dual-stage System during Seek Motion, and Control for Track Pull-in Enhancement (탐색 과정시 2단 액츄에이터의 상호 작용 분석 및 트랙 끌어들임 성능 향상을 위한 제어)

  • Choi, Jin-Young;Park, Tae-Wook;Yang, Hyun-Seok;Park, No-Cheol;Park, Young-Pil
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
    • /
    • 2004.11a
    • /
    • pp.236-241
    • /
    • 2004
  • In this paper, the dual stage interaction between the coarse actuator and the fine actuator of an optical disk drive and the control method to enhance the track pull-in performance are discussed. First, the interaction analyses for the dual stage, and the experiments to find the each actuator dynamics are studied. From the experiments results, some physical parameters was derived, then, some simulations are performed to find the residual vibration effect of the fine actuator during seek motion. Second, the fine actuator control method to reduce the relative velocity, which is a key factor in track pull-in performance, between the target track and beam spot is proposed. From simulations, we show that fine actuator control which has same frequency and same phase of the disturbance is effective to reduce the relative velocity, hence, the control method is good approach in the track pull-in enhancement. Finally, the some comments are discussed briefly.

  • PDF

Real-Time Object Recognition for Children Education Applications based on Augmented Reality (증강현실 기반 아동 학습 어플리케이션을 위한 실시간 영상 인식)

  • Park, Kang-Kyu;Yi, Kang
    • Journal of Korea Multimedia Society
    • /
    • v.20 no.1
    • /
    • pp.17-31
    • /
    • 2017
  • The aim of the paper is to present an object recognition method toward augmented reality system that utilizes existing education instruments that was designed without any consideration on image processing and recognition. The light reflection, sizes, shapes, and color range of the existing target education instruments are major hurdles to our object recognition. In addition, the real-time performance requirements on embedded devices and user experience constraints for children users are quite challenging issues to be solved for our image processing and object recognition approach. In order to meet these requirements we employed a method cascading light-weight weak classification methods that are complimentary each other to make a resultant complicated and highly accurate object classifier toward practically reasonable precision ratio. We implemented the proposed method and tested the performance by video with more than 11,700 frames of actual playing scenario. The experimental result showed 0.54% miss ratio and 1.35% false hit ratio.