Journal of Dairy Science and Biotechnology
- Volume 30 Issue 2
- /
- Pages.131-137
- /
- 2012
- /
- 2733-4554(pISSN)
- /
- 2733-4562(eISSN)
MALDI-TOF MS System for the Identification of Microorganisms in Milk and Dairy Products
우유 및 유제품 중 미생물 동정을 위한 MALDI-TOFMS활용
-
Kim, Hyoun Wook
(National Institute of Animal Science, RDA)
;
-
Ham, Jun-Sang
(National Institute of Animal Science, RDA)
;
- Seol, Kuk-Hwan (National Institute of Animal Science, RDA) ;
- Han, Sangha (National Institute of Animal Science, RDA) ;
-
Park, Beam Young
(National Institute of Animal Science, RDA)
;
-
Oh, Mi-Hwa
(National Institute of Animal Science, RDA)
-
김현욱
(농촌진흥청 국립축산과학원)
;
-
함준상
(농촌진흥청 국립축산과학원)
;
- 설국환 (농촌진흥청 국립축산과학원) ;
- 한상하 (농촌진흥청 국립축산과학원) ;
-
박범영
(농촌진흥청 국립축산과학원)
;
-
오미화
(농촌진흥청 국립축산과학원)
- Received : 2012.10.30
- Accepted : 2012.11.20
- Published : 2012.11.30
Abstract
Rapid and reliable identification of microorganisms is a key for tracing the relationship between the target bacteria and related infectious diseases. Various identification methods such as classical phenotypic analysis, numerical taxonomic analysis, and DNA sequencing have been widely used to classify microorganisms in milk and dairy products. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) identifies targeted bacteria in milk and milk products. Several studies have demonstrated that MALDI-TOF MS identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. MALDI-TOF MS could provide accurate identification of bacteria in milk and milk products at the serotype or strain level and enable antibiotic resistance profiling within minutes.