• Applied Biological Chemistry
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• v.39 no.4
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• pp.260-264
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• 1996

Process of particle bombardment for efficient transformation of Cymbidium virescence rhizome microcross sections was investigated using Biolistic particle delivery system with pBI121 harboring the ${\beta}-glucuronidase$(GUS) and the neomycin phosphotransferaseII(nptII). The best result was obtained from the combination of $1.11{\;}{\mu}m$ tungsten particles coated with pBl121, $77.33kg/cm^2$ helium pressure, 6.35 mm gap distance, and 7.0 cm target distance. Transient expression of the reporter gene, GUS, bombarded into the rhizome microsections was observed by the histochemical assay. The marker gene, nptII, delivered by bombarding the tungsten particles coated with the plasmid DNA was identified in the transformed rhizome by polymerase chain reaction.

• International Journal of Naval Architecture and Ocean Engineering
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• v.5 no.2
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• pp.188-198
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• 2013

In constructing a collision avoidance system, it is important to determine the time for starting collision avoidance maneuver. Many researchers have attempted to formulate various indices by applying a range of techniques. Among these indices, collision risk obtained by combining Distance to the Closest Point of Approach (DCPA) and Time to the Closest Point of Approach (TCPA) information with fuzzy theory is mostly used. However, the collision risk has a limit, in that membership functions of DCPA and TCPA are empirically determined. In addition, the collision risk is not able to consider several critical collision conditions where the target ship fails to take appropriate actions. It is therefore necessary to design a new concept based on logical approaches. In this paper, a collision ratio is proposed, which is the expected ratio of unavoidable paths to total paths under suitably characterized operation conditions. Total paths are determined by considering categories such as action space and methodology of avoidance. The International Regulations for Preventing Collisions at Sea (1972) and collision avoidance rules (2001) are considered to solve the slower ship's dilemma. Different methods which are based on a constant speed model and simulated speed model are used to calculate the relative positions between own ship and target ship. In the simulated speed model, fuzzy control is applied to determination of command rudder angle. At various encounter situations, the time histories of the collision ratio based on the simulated speed model are compared with those based on the constant speed model.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.6
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• pp.943-948
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• 2022

Drones have been widely used in various fields due to the rapid development of done-related technologies, which causes various problems. The schemes which can track target drones by using signal transmitted by target drones have been investigated as a key technology for anti-drone systems to solve these problems. In this paper, we investigate an illegal drone tracking system based on swarming flight that consists of multiple small drones in order to resolve the limitations of a conventional system that consists of a single drone. In addition, we also propose a scheme with which we can adaptively adjust the separation distance between small drones in a swarm according to channel situations. We analyzed the performance of the proposed scheme in terms of success ratio and the number of movements. The proposed scheme can improve the success ratio and the number of movements by 170% and 63% respectively, compared to the conventional scheme.

• Journal of the Korean Society of Visualization
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• v.20 no.3
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• pp.136-145
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• 2022

Later flow immunoassay (LFIA) is a protein analytical method based on immunoreaction. On the LFIA based protein analytical method, bioreceptor molecule plays a key role, and so a system that evaluates and manages the binding affinity of bioreceptor is needed to secure detection reliability. In this study, Lateral Flow Immunoassay based rapid Bioreceptor Screening Method (rBSM) is presented that provide a simple and quick evaluating method for the binding affinity to the target protein of the antibody as model bioreceptor. To verify this evaluation method, Virus-like particles (VLP) and anti-VLP antibodies are selected as a model norovirus, which is target protein, and the candidate bioreceptors respectively. Among the 5 different candidate antibodies, appropriate antibody could be sorted out within 30 minutes through rBSM. In addition, selected antibodies were applied to two representative LFIA based techniques, sandwich assay and competitive assay. Among these methods, sandwich assay showed more effective VLP detection method. Through applying selected antibodies and techniques to the commercialized mass production lines, an VLP detecting LFIA kit was developed with a detection limit of 10¹² copies/g of VLPs in real samples. Since this proposed method in this study could be easily transformable into other combinations with bioreceptors, it is expected that this technique would be applied to LFIA kit development system and bioreceptor quality management.

• 재활복지
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• v.21 no.2
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• pp.1-27
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• 2017

The purpose of this study is to evaluate the level of implementation of the Incheon Strategy and to develop measures for effective implementation. This research method used literature review and monitoring method. The results of the study are summarized as follows: First, 9 key indicators and 7 supplementary indicators, which are classified into implementation and non-implementation, 3 key indicators have been implemented, one indicator has been partially implemented, 5 indicators have not yet been implemented, Also, In the case of supplementary indicators, five were implemented and two were not. Second, the ten target areas are lacking in implementation, but the objective of 7 is to ensure the comprehensive disaster risk reduction and management, the ratification and implementation of the Convention on the Rights of Persons with Disabilities and the harmonization of the Convention with the domestic law, Also, it is analyzed that the level of implementation is in the order of improving the reliability and comparability of the data of goal 8, ensuring gender equality of goal 6 and strengthening the capacity of women. Based on these results, we propose an improvement plan for implementation. First, it is necessary to formulate policy issues and implement measures for the implementation of Incheon Strategy. Second, it is necessary to establish a system to implement and monitor for Incheon strategy. Third, Korean standards for goals and targets, key indicators and supplementary indicators are needed. Fourth, it is necessary to prioritize the target implementation and to take preemptive action. Lastly, it is necessary to educate and publicize for the Incheon strategy.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.4
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• pp.505-512
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• 2002

Fast spreading of web made we get easily the vast amount of information all over the world, but quantity of great information on the Internet space is giving much troubles to recognize change of information that users are interested soon justly. That is, users must connect and examine one by one to relevant site to detect change of web documents that changes from time to time. Therefore, the development of Robot which accomplish Information change monitoring function that sense automatically changed contents and inform to user is required. In this paper, we designed and implemented Web site contents change monitoring system, which notify-automatically the change of Web documents to users through alarm or E-mail if user defines target URL to do monitoring, monitoring condition, monitoring period etc. And we presented the method that structure and classify Web Documents to semantic units using HTML Tag. Also, we introduced the concept of virtual key to manage position of word to watch some change efficiently.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.173.1-173.1
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• 2011

The high fuel flexibility of gas turbine power system has boosted their use in a wide variety of applications. Recently, the demand for biogas generated from the digestion of organic wastes and sewage waste water as a fuel for gas turbines has increased. We investigated the performance of high pressure biogas compression system and operating conditions for supplying biogas. The total flow per minute of biogas from food waste water digestion tank is $54Nm^3$. The main type of biogas compression system is the reciprocating system and screw type system. The target of biogas mechanical data is the as belows; inlet pressure 0.045bar, supplying biogas temperature is $30{\sim}60^{\circ}C$, and final pressure is above the 25 bar. Also, inlet conditions of biogas consist of CH4 48.5%~83%, $H_2S$ Max. 500ppm, $NH_3$ Max. 1,500ppm and Siloxane 2.7~4.6ppm. The boosting Blower system raises a pressure from 0.045bar to 1bar before main compressor. The main system lay out of reciprocating consisits of compressor driver, filter, cooling system, blowdown vessel, control system and ESD(Emergency Shut Down) system. And an enclosure package needs to be installed for reducing noise up to 75dB. The system driver is the electronic motor of explosion proof type. Forthe compressor system reliable operation, the cleaning system something like particulate filter needs to be set up in the inlet of compressor and Coalescing Filter in the outlet of compressor. Particulate Filter has to be removed above $10{\mu}m$ size of the particles in biogas. The coalescing filter(Micofine Borosilicate Glass Fibers Filter treated phenol acid) also removes moisture and oil of above $0.3{\mu}m$ to be involved in high pressure biogas up to 90%~98%.

• The Journal of the Convergence on Culture Technology
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• v.10 no.1
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• pp.411-417
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• 2024

In the event of a disaster, it is essential to establish a disaster recovery plan and disaster recovery system to minimize disruption to major IT infrastructure and provide continuous business services. In the process of building a disaster recovery system, data replication is a key element of data recovery to provide uninterrupted and continuous business services in the event of a disaster. The data replication method can be determined depending on the system configuration environment and disaster recovery goal level. In this paper, we present a method for determining a data replication method suitable for the configuration environment and disaster recovery target level when building a disaster recovery system. In addition, the replication method decision procedure is applied to build a disaster recovery system and analyze the construction results. After establishing the disaster recovery system, a test was conducted to determine whether the service was transferred to the disaster recovery center in a disaster situation and normal service was provided, and the results were analyzed. As a result, it was possible to systematically select the optimal data replication method during the disaster recovery system construction phase. The established disaster recovery system has an RTO of 3.7 hours for service conversion to the disaster recovery center to provide continuous business services, and the disaster recovery level, which was Tier 2, has been improved to the target level within 4 hours of RTO and RPO=0.

• Biomolecules & Therapeutics
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• v.15 no.1
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• pp.16-26
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• 2007

Tissue plasminogen activator (tPA) is a serine protease catalyzing the proteolytic conversion of plasminogen into plasmin, which is involved in thrombolysis. During last two decades, the role of tPA in brain physiology and pathology has been extensively investigated. tPA is expressed in brain regions such as cortex, hippocampus, amygdala and cerebellum, and major neural cell types such as neuron, astrocyte, microglia and endothelial cells express tPA in basal status. After strong neural stimulation such as seizure, tPA behaves as an immediate early gene increasing the expression level within an hour. Neural activity and/or postsynaptic stimulation increased the release of tPA from axonal terminal and presumably from dendritic compartment. Neuronal tPA regulates plastic changes in neuronal function and structure mediating key neurologic processes such as visual cortex plasticity, seizure spreading, cerebellar motor learning, long term potentiation and addictive or withdrawal behavior after morphine discontinuance. In addition to these physiological roles, tPA mediates excitotoxicity leading to the neurodegeneration in several pathological conditions including ischemic stroke. Increasing amount of evidence also suggest the role of tPA in neurodegenerative diseases such as Alzheimer's disease and multiple sclerosis even though beneficial effects was also reported in case of Alzheimer's disease based on the observation of tPA-induced degradation of $A{\beta}$ aggregates. Target proteins of tPA action include extracellular matrix protein laminin, proteoglycans and NMDA receptor. In addition, several receptors (or binding partners) for tPA has been reported such as low-density lipoprotein receptor-related protein (LRP) and annexin II, even though intracellular signaling mechanism underlying tPA action is not clear yet. Interestingly, the action of tPA comprises both proteolytic and non-proteolytic mechanism. In case of microglial activation, tPA showed non-proteolytic cytokine-like function. The search for exact target proteins and receptor molecules for tPA along with the identification of the mechanism regulating tPA expression and release in the nervous system will enable us to better understand several key neurological processes like teaming and memory as well as to obtain therapeutic tools against neurodegenerative diseases.

• Journal of the Korea Institute of Military Science and Technology
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• v.19 no.6
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• pp.698-702
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• 2016

Thermal imager is now regarded as one of the key observation devices for ISR activities and getting important more and more. As other detectors, however, the thermal detectors also have maximum input and therefore they will be saturated if the input IR energy exceeds the allowed range. The saturation in the thermal detector makes it impossible to distinguish the target from background, as a result, the thermal imager does not perform its own mission anymore. In order to get an insight related with the image saturation, this paper develops a saturation model for a thermal imaging system, not a thermal detector. The proposed modeling starts from analyzing the specification of a thermal imager. Coupled with the characteristic parameters of the object, the saturation model can be used to predict the distance on which the detector is saturated. The proposed saturation model prove its validity by applying it for the case of observing a flash-bang.