• Asian Journal for Public Opinion Research
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• 제5권4호
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• pp.319-345
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• 2018

Effective higher educational management in undergraduate nursing programs is an important issue from the viewpoint of stakeholders. This qualitative research aimed to examine the characteristics of nursing students and curriculum development of undergraduate nursing education from the opinions of Boromarajonani College of Nursing Saraburi, Thailand stakeholders. The population included 4 groups: 1) the alumni who have graduated within the past 5 years and currently work in primary, secondary, and tertiary care units, 2) the supervisors and colleagues of the alumni, 3) nursing lecturers, and 4) the current nursing students. The respondents who are the alumni, nursing lecturers, and current nursing student were selected using a purposive sampling, for the supervisors and colleagues were selected using snowball techniques. Semi-structured interview questions were used for data collection. Group discussions were conducted until saturation on 55 key informants. The qualitative data was analyzed using content analysis. Results showed the viewpoints of stakeholders on the characteristics of future nurse graduates were comprised of four elements: knowledge that meets standards; essential skills for self-development and lifelong learning process; good morals and professional ethics in providing nursing care; and nurse competencies in teamwork, communication, language, research, management, IT, life skills, and global literacy. The viewpoints on the development of the nursing curriculum focus on four elements: the learner, teaching and learning, course content, and instructor tasks. For learners, the admission criteria should include a minimum not only of knowledge, but also positive attitude, science, and art skills, since the nursing profession is both a science and the art of caring. Teaching and learning elements should be authentic, including exposure to real situations, an integrated network, and activities that improve nursing care. Course content was comprised of an updated curriculum, humanized nursing care, student center, theory and practice with moral integration, case-based study, critical thinking, multidisciplinary work, and love for the nursing profession. Instructor tasks are to elicit student ideas, provide opportunities to learn, support infrastructure, support technology use, and extra-curricular activities to develop the competencies of nursing students. Recommendations were that the curriculum administration should review the selection process of student candidates and instructional management to achieve expected outcomes of nursing characteristics in the future. The nurse lecturer should provide authentic and integrated instruction, decrease lecturing, cultivate a lifelong learning process, and sustain the nursing characteristics.

• 원예과학기술지
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• 제33권6호
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• pp.923-931
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• 2015

본 연구는 일시적인 고온 스트레스 처리가 복색 장미 'Pinky Girl'의 화아 착색과 안토시아닌 생합성 관련 유전자들의 발현 양상에 미치는 영향을 알아보고자 수행되었다. 꽃잎에서 cyanin의 축적은 화아의 발육단계와 연관이 있으며 화아 발육 과정에서 꽃잎이 출현하는 4단계($S_4$)에서 급격하게 일어났다. 따라서 $S_4$ 단계가 꽃잎 착색에 가장 민감한 시기로 판단된다. $S_4$ 단계에서 3일간 고온 스트레스($39/18^{\circ}C$)를 받은 고온처리구에서 개화 당시 꽃잎의 cyanin 착색이 대조구와 비교하여 45.5% 감소하였다. 한편, 안토시아닌 생합성 관련 유전자인 CHS, CHI, F3'H, DFR, ANS, 3GT, 5GT의 발현은 고온처리구에서 오히려 촉진되었다. 예외적으로 F3H의 발현은 고온처리구에서 26.7% 감소하여 'Pinky Girl'의 복색 발현에 의미 있는 유전자로 확인되었다. 하지만 대부분 안토시아닌 생합성 관련 유전자들의 발현은 꽃잎에서의 cyanin 착색 경향과 비례적이지 않았다. 따라서 장미 꽃잎의 화색소 축적은 번역 이후 효과와 조절유전자와 같은 안토시아닌 생합성과 관련된 다른 복잡한 메커니즘이 연관되어 있을 것으로 판단되었다.

• Toxicological Research
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• 제11권1호
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• pp.1-8
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• 1995

Fumonisins are a family of mycotoxins produced by the fungus Fusarium moniliforme which is a common contaminant in corn. Fumonisins are potent inhibitors of sphingosine and sphinganine N-acyltransferase (ceramide synthase), key enzymes in sphingolipid metabolism. The purpose of this study was to provide the evidence that the elevated levels of free sphingoid bases (primarily sphinganine) and depletion of complex sphingolipids were closely related to the inhibition of cell growth in LLC-$PK_1$ cells exposed to fumonisin $B_1$$(\leq 35 {\mu}M)$. Concentrations of fumonisin $B_1$ between 10 and $35 {\mu}M$ were known to inhibit cell growth without cytotoxicity in $LLC-PK_1$ cells (Yoo et al. Toxicol. Appl. Pharmacol. 114, 9-15, 1992). Cells exposed to 35$\mu M$ fumonisin B$_1$ for 48 and 72 hr developed a fibroblast-like (elongated and spindle-shaped) appearance and were less confluent than normal cells. At between 24 and 48 hr after exposure to fumonisin $B_1$ cells were beginning to show the inhibition of cell growth and at 72 hr the number of viable cells in fumonisin-treated cultures was about 50% of concurrent control cultures. During the 24 hr lag period preceding inhibition of cell growth, the free sphinganine levels in cells exposed to $35 {\mu}M$ fumonisin $B_1$ were highly elevated (approximately 230 fold higher than normal cells). The elevated levels of free sphinganine were $435\pm14$$pmoles/{10^6}$ cells at 48 hr and approximately TEX>$333\pm11$$pmoles/{10^6}$ cells in cells exposed to $35{\mu}M$ fumonisin$B_1$ at 72 hr, while the levels of free sphinganine in normal cells were less than 2$pmoles/{10^6}$ cells. Under the same condition, depletion of intracellular complex sphingolipids as a consequence of fumonisin inhibition of de novo sphingolipid biosynthesis and turnover pathway was appeared. Content of free sphingold bases in dividing cells was more elevated than in confluent cells at 24-48 hr after cells were exposed to $20{\mu}M$ fumonisin $B_1$. The dividing cells were showing the inhibition of cell growth at 48-72 hr and $20{\mu}M$ fumonisin $B_1$. The results of this study support the hypothesis that the inhibition of cell growth is very well related to the disruption of sphingolipid metabolism in $LLC-PK_1$ cells.

• 동의신경정신과학회지
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• 제19권3호
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• pp.179-193
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• 2008

Objective: The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H202-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods: The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions: 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions. 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

• 한국인쇄학회지
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• 제31권1호
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• pp.39-49
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• 2013

Nowadays the printing market has shift from 2D(two dimension) industry to 3D(three dimension) industry, along with IT. With rapidly changing market, it's true that the effort to develop the printing materials and upgrade the printing qualities is produced actively in the country. Particularly, the differences in printing materials can often determine the quality of printings in the printability. In the case of the printability of offset, 9 items(paper, ink, order of printing, ink supply, blowing-up halftone, grey balance, key color(base color), overlap efficiency, quality at the halftone dot reproduction etc.) are usually considered as the important factors and used to estimate the printability at abroad. However, in domestic, ink-jet paper is not used for the printing/publishing by ink-jet printing for digital, but done mainly for offset printing. Because the paper of gloss coated type, which is the ink-jet paper, has high price, it's too hard to manufacture massively printing/publishing. Also, in the case of the paper of uncoated type, in spite of its reasonable price rather than the former, the type of uncoated one also has demerit that the ink-jet paper has a clear difference between the front and the back. Therefore, it's tough reality that Ink Jet paper is hardly taken advantage of as the printing/publishing. Thus, what is done in the first to print and/or publish on ink-jet paper is that the front and the back must be printed evenly, and appropriate to the printability of ink-jet. Although there're some kinds of ink-jet paper in the country, it has not been produced in true domestic production yet. In addition, thanks to the brightness of fluorescence brightening agent, the foreign product can offer the good-qualified one-time printing. But when it comes to the printing/publishing, there're difficulties that it may cause the Ocular Fatigue because of long-term exposure of the eyes to text. This research tries to find out the optimum ink-jet paper in domestic for the printing/publishing by studying on the printability of ink-jet on digital web-printing. And it also tries to look for the paper's brightness which can be reduce the possibility to cause the consumers' Ocular Fatigue.

• 한국지구과학회지
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• 제24권8호
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• pp.657-667
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• 2003

현세-후기 플라이스토세의 부정합적 경계면을 조사하기 위하여 한국 서해 경기만 조간대에서 심부시추와 탄성파 탐사를 실시하였다. 분석된 모든 시추 퇴적물에서 현세 퇴적층(Unit I) 하부에 놓이는 최대 4m두께의 산화되고, 반고화된 특징을 보이는 황갈색의 산화대층(oxidized-sedimentary layer)이 발견되었다. 이 세립질의 산화대층에서 나타나는 황갈색의 퇴적물 색, 높은 N 값과 낮은 함수율의 준고화된 상태, 동토구조, 스멕타이트 광물의 부재 그리고 높은 퇴적물 화학적 풍화지수(Ba/Sr 비) 등의 다양한 특성은 퇴적물의 대기중 노출과 풍화의 중요한 증거로 제시된다. 탄소동위원소 연대와 함께 이러한 여러 증거들을 고려할 때, Unit II의 상부 산화대층은 초기 현세까지 계속되는 저해수면 동안 대기중에 노출되어 풍화 및 산화작용으로 인하여 퇴적물의 특성이 변질되어 형성된 것으로 해석된다. 따라서 산화대층의 상부 경계면은 현세와 선현세(후기 플라이스토세) 퇴적층을 구분하는 부정합면으로 제시되며, 탄성파 자료에서 나타나는 강한 반사면(prominent near-surface reflector)과 잘 일치한다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1519-1529
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• 2016

Background: Matrix metalloproteinase -2 (gelatinase-A, Mr 72,000 type IV collagenase, MMP-2) and -9 (gelatinase-B, Mr 92,000 type IV collagenase, MMP-9) are key molecules that play roles in tumor growth, invasion, tissue remodeling, metastasis and stem-cell regulation by digesting extracellular matrix barriers. MMP-2 and -9 are well known to impact on solid cancer susceptibility, whereas, in hematological malignancies, a paucity of data is available to resolve the function of these regulatory molecules in bone marrow mononuclear cells (BM-MNCs) and stromal cells of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Objectives: The present study aimed to investigate mRNA expression and gelatinase A and B secretion from BM-MNCs in vitro and genotypic associations of MMP-2 (-1306 C/T; rs243865), MMP-9 (-1562 C/T; rs3918242), tissue inhibitor of metalloproteinase -1 (TIMP-1) (372T/C; rs4898, Exon 5) and TIMP-2 (-418G/C; rs8179090) in MDS and AML. Results: The study covered cases of confirmed MDS (n=50), AML (n=32) and healthy controls (n=110). MMP-9 mRNA expression revealed 2 fold increased expression in MDS-RAEB II and 2.5 fold in AML M-4 (60-70% blasts). Secretion of gelatinase-B also revealed the MMP-9 mRNA expression and ELISA data also supported these data. We noted that those patients having more blast crises presented with more secretion of MMP-9 and its mRNA expression. In contrast MMP-9 (-1562 C/T) showed significant polymorphic associations in MDS (p<0.02) and AML (p<0.02). MMP-9 mRNA expression of C/T and T/T genotypes were 1.5 and 2.5 fold increased in MDS and AML respectively. In AML, MMP-2 C/T and T/T genotypes showed 2.0 fold mRNA expression. Only MMP-9 (-1306 C/T) showed significant 4 fold (p<0.001) increased risk with chemical and x-ray exposed MDS, while tobacco and cigarette smokers have 3 fold (p<0.04) risk in AML. Conclusions: In view of our results, MMP-9 revealed synergistic secretion and expression in blast crises of MDS and AML with 'gene' polymorphic effects and is significantly associated with increased risk with tobacco, cigarette and environmental exposure. Release and secretion of these enzymes may influence hematopoietic cell behavior and may be important in the clinical point of view. It may offer valuable tools for diagnosis and prognosis, as well as possible targets for the treatments.

• Toxicological Research
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• 제22권1호
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• pp.23-30
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• 2006

Among poly aromatic hydrocarbons, dioxin and PCBs are the most controversial environmental pollutants in our modern life. These pollutants are known as human carcinogens, and liver is the most sensitive target in animal cancer models. Specific aims of the study were focused on the mechanism of carcinogenesis in hepatocytes and the structure-activity relation among these diverse environmental chemicals. Because key mechanisms of dioxin-induced carcinogenesis in human epithelial cell model are the alteration of signal transduction pathway and PKC isoforms, the alteration of the signal transduction pathways and other factors associated with carcinogenesis were studied. Rat hepatocytes cultured under the sandwich protocols were exposed with the various concentration of dioxins and PCBs, and signal transduction pathway, protein kinase C isoforms, oxidant stress, and apoptotic nuclei were evaluated. Since it is important to understand the structure-activity relation among these chemicals to properly assess the carcinogenic potentials, the study analyzed the parameters associated with carcinogenic processes, based on their structural characteristics. In addition, signal transduction pathways and PKC isoforms involved in inhibition of UV-induced apoptosis were also analyzed to elaborate the tumor promotion mechanism of these chemicals. Induction of apoptosis by UV irradiation was optimal at $60\;J/m^2$ in primary hepatocyte in culture. Compared to non coplanar PCBs such as PCB 114 and PCB 153, coplanar PCBs such as PCB 77 and PCB126 showed a stronger inhibition of apoptosis induced by UV irradiation. Production of reactive oxygen species (ROS) was more stimulated by non-coplanar PCBs than coplanar PCBs with the most potent induction of ROS by chlorinated non-coplanar PCB. As compared to the level of induction by PCB126, non-coplanar PCB153 showed a higher increase of intracellular concentrations. Besides the alteration of intracellular calcium concentration, translocation of PKC from cytosolic fraction to membrane fraction was clearly observed upon the exposure of non-coplanar PCB. Taken together, the present study demonstrated that there is a potent structure-activity relationship among PCB congeners and the mechanism of PAH-induced carcinogenesis is structure-specific. The study suggested that more diverse pathways of PAH-induced carcinogenesis should be taken into account beyond the boundary of Ah receptor dogma to assess the health impact of PAH with more accuracy.

• 한국강구조학회 논문집
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• 제27권2호
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• pp.143-153
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• 2015

본 연구에서는 매립형 합성보와 슬림플로어보의 내화성능평가를 위한 재하가열실험 및 수치해석연구를 수행하였다. 이를 통해 화재 시 합성보의 온도 및 처짐 변화를 분석하고 하중비, 철근, 화재노출면 등의 합성보 설계변수들이 내화성능에 미치는 영향을 비교하였다. 화재실험결과 매립형 합성보와 슬림플로어보의 경우 강재의 온도상승이 일반형 합성보에 비해 상당히 지연됨을 확인하였다. 그리고 매립콘크리트 내에 보강된 철근은 최소 90분까지는 상온강도를 발휘할 수 있는 온도이하를 유지하였다. 무보강 매립형 합성보, 철근을 보강한 매립형 합성보와 슬림플로어보의 경우 처짐량 제한 기준만으로도 2시간 이상의 내화성능을 달성하였다. 이는 무피복 매립형 합성보와 슬림플로어보가 강재보의 내화성능을 증진하는 합리적인 대안이 될 수 있음을 시사한다. 합성보 재하가열실험에 대하여 ABAQUS를 활용한 유한요소해석을 수행한 결과 전체적인 처짐 양상 및 내화시간의 예측에 있어서 해석결과가 실제 실험결과를 신뢰성 있게 구현하고 있음을 확인하였다.

• 과학기술학연구
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• 제18권1호
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• pp.129-175
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• 2018

이 논문은 한국석면운동의 지식 정치적 측면을 검토한다. 2008년 본격적으로 시작된 석면운동은 석면오염을 한국 사회의 중요한 안전보건의제로 만드는 데 앞장서 왔다. 석면오염 측정은 특히 석면오염이 광범위하고 심각한 환경보건문제라는 주요 주장을 펼치는 데 매우 중요했다. 이 활동의 특징은 공정시험방법이 아닌 '먼지 분석법'을 활용했다는 것이다. 이 논문에서는 석면운동가들의 오염 조사 보고서와 삼성 본관 주변 석면오염 논란을 통해 먼지 분석법이 대항 지식으로서 권력을 획득하는 과정을 분석한다. 석면운동가들은 먼지 분석으로 얻은 측정 데이터를 활용해 그들의 주장에 언론의 이목을 집중시키고, 정책적 변화에 필요한 자원을 동원할 수 있었다. 그러나 규제기관을 중심으로 한 전문가 집단은 먼지 분석법이 신뢰할 수 있는 석면오염 측정방법이라는 석면운동가들의 주장에 동의하지 않았다. 이와 같은 결론을 내리는 데 측정 과정에서 표준적 방법의 사용 여부와 측정 데이터의 해석적 맥락이 중요한 기준으로 작용하였다. 먼지 분석법의 권위는 부분적으로 획득되었다. 그러나 먼지 분석법은 규제과학이 내포하는 가정들을 드러내고 사회적 토론의 대상으로 전환시켰다는 점에서, 사회 운동과 과학을 결합하여 규제기관과 전문가 집단의 측정에 도전한 석면운동의 특성을 잘 보여준다.