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Profiles of Bacillus spp. Isolated from the Rhizosphere of Suaeda glauca and Their Potential to Promote Plant Growth and Suppress Fungal Phytopathogens

  • Lu, Ping;Jiang, Ke;Hao, Ya-Qiao;Chu, Wan-Ying;Xu, Yu-Dong;Yang, Jia-Yao;Chen, Jia-Le;Zeng, Guo-Hong;Gu, Zhou-Hang;Zhao, Hong-Xin
    • Journal of Microbiology and Biotechnology
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    • v.31 no.9
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    • pp.1231-1240
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    • 2021
  • Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.

Exploratory Analysis of Consumer Responses to Korea-China Mobile Payment Service using Keyword Analysis -Focus on Kakao Pay and Alipay- (키워드 분석을 활용한 한·중 모바일 결제 서비스에 대한 소비자 반응 탐색적 분석 -카카오페이와 알리페이를 중심으로-)

  • Ke, Jung;Yoon, Donghwa;Ahn, Jinhyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.6
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    • pp.514-523
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    • 2021
  • Recently, the proliferation of mobile simple payment services has been increasingly affecting people's lives. In addition, the increase in research from both China and Korea shows that the continuous development of simple mobile payment services will be very important in the future. The blog posts mentioning Kakao Pay and Alipay were collected, and keyword analysis was performed to investigate differences in consumers' responses to Kakao Pay and Alipay on social media. The frequency of keywords for each part of speech and the frequency of co-occurred words mentioned in one sentence were analyzed. Specifically, common words that appear in both Kakao Pay and Alipay blogs were extracted. The cooccurred words were analyzed to examine how different reactions were made on the same subject. As a result of the analysis, there were concerns among consumers about the trust of Kakao Pay and Alipay's benefits. For a mobile payment service to become competitive, it is necessary to add various additional services or solve security problems.

The Study on Modern Neo-Confucianism in China : Accepting and Understanding Modern Neo-Confucianism in China (중국의 현대신유학 수용과 이해 - 1980년대 현대신유학 연구를 중심으로 -)

  • Park, Young-Mi
    • The Journal of Korean Philosophical History
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    • no.23
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    • pp.349-392
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    • 2008
  • Modern Neo-Confucianism was formed as a school by solving the modern problems in China through accepting western philosophies with Chinese basic philosophies since New Cultural Movement. Marxism, Liberalism, and Modern Neo-Confucianism are called three representatives of Chinese modern philosophies. Since the People's Republic of China was founded in 1949, Modern Neo-Confucianists have tried to keep their philosophy and cultural conservatism in Hong Kong and Taiwan. Modern Neo-Confucianism which had been prohibited before 1978 was brought again to people's attention in the middle of 1980s by their active lectures and writings. Furthermore, the study on Modern Neo-Confucianism was supported by the Chinese government in 1987. China was trying to find the way to enhance Chinese tradition and to develop China to a modern society at the same time through the study on Modern Neo-Confucianism. The purpose of Modern Neo-Confucianism is to keep Chinese tradition which was broken off, to develop China to a modern society, to control the problems caused by capitalism socially, and ultimately to strengthen socialism in China in the political aspect. The study on Modern Neo-Confucianism in the 1980s focused on introducing, organizing, and understanding Modern Neo-Confucianism as its early stage. This study was led by Marxists with their methods and viewpoints. Even though the acceptance and understanding of Modern Neo-Confucianism was limited in a short period, the study on Modern Neo-Confucianism in the 1980s propagated Modern Neo-Confucianism. Modern Neo-Confucianism also played an important role to grow the argument about the critical succession of Chinese tradition and to reconsider the fact that modernization does not mean only westernization.

Efficacy of Different Number of XELOX or SOX Chemotherapy Cycles After D2 Resection for Stage III Gastric Cancer

  • Yu, Yuanyuan;Zhang, Zicheng;Meng, Qianhao;Wang, Ke;Li, Qingwei;Ma, Yue;Yao, Yuanfei;Sun, Jie;Wang, Guangyu
    • Journal of Gastric Cancer
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    • v.22 no.2
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    • pp.107-119
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    • 2022
  • Purpose: We aimed to explore whether the prognosis of patients treated with capecitabine and oxaliplatin (XELOX) or S-1 and oxaliplatin (SOX) regimens who received fewer cycles of chemotherapy after D2 radical resection for gastric cancer (GC) would be non-inferior to that of patients who received the standard number of cycles of chemotherapy. Materials and Methods: Data on patients who received XELOX or SOX chemotherapy after undergoing D2 radical resection at Harbin Medical University Cancer Hospital between January 2011 and May 2016 were collected. Results: In patients who received 4, 6, and 8 cycles of chemotherapy, the 5-year overall survival (OS) rates were 59.4%, 64.8%, and 62.7%, respectively. Compared to patients who received 4 cycles of chemotherapy, those who received 6 cycles (hazard ratio [HR], 0.882; 95% confidence interval [CI], 0.599-1.299; P=0.52) or 8 cycles (HR, 0.882; 95% CI, 0.533-1.458; P=0.62) of chemotherapy did not exhibit significantly prolonged OS. The 3-year disease-free survival (DFS) rate of patients who received 4, 6, and 8 cycles of chemotherapy was 62.1%, 67.2%, and 60.8%, respectively. Compared to patients who received 4 cycles of chemotherapy, those who received 6 cycles (HR, 0.835; 95% CI, 0.572-1.221; P=0.35) or 8 cycles (HR, 0.972; 95% CI, 0.606-1.558; P=0.91) of chemotherapy did not show significantly prolonged DFS. However, the 3-year DFS and 5-year OS rates of patients who received 6 cycles of chemotherapy appeared to be superior to those of patients who received 4 and 8 cycles of chemotherapy. Conclusions: For patients with stage III GC, 4 to 6 cycles of XELOX or SOX chemotherapy may be a favorable option. This study provides a rationale for further randomized clinical trials.

A Study on the Factors Affecting the Intention of Chinese Users to Discriminate Against Fake News on Social Media - Focusing on attitude, social capital, and risk detection - (중국 이용자 소셜미디어 가짜뉴스 판별의도에 미치는 요인에 관한 연구 -태도, 사회자본, 위험감지를 중심으로-)

  • Tan, KeHong;Lee, Hwa Haeng
    • The Journal of the Korea Contents Association
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    • v.22 no.4
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    • pp.337-351
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    • 2022
  • With the full spread and rapid development of social media, the trend of decentralization of social media information propagation is becoming clearer day by day, and the segmentation of time by audiences using social media information is clearly progressing. Therefore, this study aims to study the influence relationship between social media attitudes toward fake news, social capital, risk perception, and discriminant intentions based on existing studies. Accordingly, the research model presented related research questions and organized a questionnaire to collect a total of 500 valid surveys. The SPSS 26.0 program and the AMOS 24.0 program were used to analyze the data. The research results are as follows. First, the more positive the user's attitude towards the fake news identification intention of social media, the more they want to use various methods or tools to identify the authenticity of online information. Second, the more positive the user's attitude towards social media fake news, the more aware of the potential threats social media fake news poses to their own physical, psychological, financial and so on. At the same time, by raising one's own awareness of the dangers, counterintelligence intentions against fake news on social media will also increase. Third, the richer the social capital the user has, the stronger the information literacy, and therefore the stronger the identification intention of social media fake news. Fourth, the higher the value of social capital Chinese users have, the greater the damage they have suffered from fake news, and the higher the risk awareness of fake news to protect their interests. Fifth, it means that Chinese users recognized information suspected of social media and took corresponding measures.

Measurement uncertainty analysis of radiophotoluminescent glass dosimeter reader system based on GD-352M for estimation of protection quantity

  • Kim, Jae Seok;Park, Byeong Ryong;Yoo, Jaeryong;Ha, Wi-Ho;Jang, Seongjae;Jang, Won Il;Cho, Gyu Seok;Kim, Hyun;Chang, Insu;Kim, Yong Kyun
    • Nuclear Engineering and Technology
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    • v.54 no.2
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    • pp.479-485
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    • 2022
  • At the Korea Institute of Radiological and Medical Sciences, physical human phantoms were developed to evaluate various radiation protection quantities, based on the mesh-type reference computational phantoms of the International Commission on Radiological Protection. The physical human phantoms were fabricated such that a radiophotoluminescent glass dosimeter (RPLGD) with a Tin filter, namely GD-352M, could be inserted into them. A Tin filter is used to eliminate the overestimated signals in low-energy photons below 100 keV. The measurement uncertainty of the RPLGD reader system based on GD-352M should be analyzed for obtaining reliable protection quantities before using it for practical applications. Generally, the measurement uncertainty of RPLGD systems without Tin filters is analyzed for quality assurance of radiotherapy units using a high-energy photon beam. However, in this study, the measurement uncertainty of GD-352M was analyzed for evaluating the protection quantities. The measurement uncertainty factors in the RPLGD include the reference irradiation, regression curve, reproducibility, uniformity, energy dependence, and angular dependence, as described by the International Organization for Standardization (ISO). These factors were calculated using the Guide to the Expression of Uncertainty in Measurement method, applying ISO/ASTM standards 51261(2013), 51707(2015), and SS-ISO 22127(2019). The measurement uncertainties of the RPLGD reader system with a coverage factor of k = 2 were calculated to be 9.26% from 0.005 to 1 Gy and 8.16% from 1 to 10 Gy. A blind test was conducted to validate the RPLGD reader system, which demonstrated that the readout doses included blind doses of 0.1, 1, 2, and 5 Gy. Overall, the En values were considered satisfactory.

Serum Resistance in Riemerella anatipestifer is Associated with Systemic Disease in Ducks

  • Wei, Bai;Seo, Hye-Suk;Shang, Ke;Zhang, Jun-feng;Park, Jong-Yeol;Lee, Yea-Jin;Choi, Yu-ri;Kim, Sang-Won;Cha, Se-Yeoun;Jang, Hyung-Kwan;Kang, Min
    • Korean Journal of Poultry Science
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    • v.48 no.4
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    • pp.327-335
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    • 2021
  • Riemerella anatipestifer (RA) can cause septicemia, polyserositis, and ataxia in ducks. It can also colonize the upper respiratory tract of healthy ducks. These differences in pathogenicity are probably the result of diverse mechanisms of virulence in different strains. Since serum resistance is a feature frequently found in systemic pathogens, 130 RA strains having different clinical origins were tested. A variety of serum susceptibility levels were detected. Pharynx strains from healthy ducks were mainly susceptible to the bactericidal effect of the serum, while systemic strains were serum resistant. Heat-treatment of the sera abolished the bactericidal activity, indicating that complement is a key factor in this effect. In an attempt to associate serum-resistance to surface determinant genes of the bacteria, we screened for six genes involved in lipopolysaccharide synthesis and membrane proteins in RA. Of these, three genes (AS87_09335, AS87_00480, and AS87_05195) encoding outer membrane proteins might be implicated in serum resistance statistically. The results indicate that serum resistance is a virulence mechanism in RA.

Effects of different culture systems on the culture of prepuberal buffalo (Bubalus bubalis) spermatogonial stem cell-like cells in vitro

  • Li, Ting-Ting;Geng, Shuang-Shuang;Xu, Hui-Yan;Luo, Ao-Lin;Zhao, Peng-Wei;Yang, Huan;Liang, Xing-Wei;Lu, Yang-Qing;Yang, Xiao-Gan;Lu, Ke-Huan
    • Journal of Veterinary Science
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    • v.21 no.1
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    • pp.13.1-13.14
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    • 2020
  • Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.

The Comparison of Quantitative Accuracy between Energy Window-Based and CT-Based Scatter Correction Method in SPECT/CT Images (SPECT/CT 영상에서 에너지창 기반 산란보정과 CT 기반 산란보정 방법의 정량적 정확성 비교)

  • Kim, Ji-Hyeon;Lee, Joo-Young
    • Journal of radiological science and technology
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    • v.45 no.2
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    • pp.135-143
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    • 2022
  • In SPECT image, scatter count is the cause of quantitative count error and image quality degradation. This study is to evaluate the accuracy of CT based SC(CTSC) and energy window based SC(EWSC) as the comparison with existing Non SC. SPECT/CT images were obtained after filling air in order to acquire a reference image without the influence of scatter count inside the Triple line insert phantom setting hot rod(99mTc 74.0 MBq) in the middle and each SPECT/CT image was obtained each separately after filling water instead of air in order to derive the influence of scatter count under the same conditions. For EWSC, 9 sub-energy windows were set additionally in addition to main energy window(140 keV, 20%) and then, images were acquired at the same time and five types of EWSC including DPW(dual photo-peak window)10%, DEW(dual energy window)20%, TEW(triple energy window)10%, TEW5.0%, TEW2.5% were used. Under the condition without fluctuations in primary count, total count was measured by drawing volume of interest (VOI) in the images of the two conditions and then, the ratio of scatter count of total counts was calculated as percent scatter fraction(%SF) and the count error with image filled with water was evaluated with percent normalized mean-square error(%NMSE) based on the image filled with air. Based on the image filled with air, %SF of images filled with water to which each SC method was applied is non scatter correction(NSC) 37.44, DPW 27.41, DEW 21.84, TEW10% 19.60, TEW5% 17.02, TEW2.5% 14.68, CTSC 5.57 and the scatter counts were removed the most in CTSC and %NMSE is NSC 35.80, DPW 14.28, DEW 7.81, TEW10% 5.94, TEW5% 4.21, TEW2.5% 2.96, CTSC 0.35 and the error in CTSC was found to be the lowest. In SPECT/CT images, the application of each scatter correction method used in the experiment could improve the quantitative count error caused by the influence of scatter count. In particular, CTSC showed the lowest %NMSE(=0.35) compared to existing EWSC methods, enabling relatively accurate scatter correction.

Factors Influencing Daily Time Use Types of Older Adults and Their Effect on Life Satisfaction: A Study using Latent Profile Analysis (LPA) (잠재프로파일(LPA) 분석을 활용한 노인의 생활시간 유형의 영향요인과 유형에 따른 생활만족도)

  • Nam, Seok In;Chae, Joosuk;Won, Hae Sol;Lee, Ju Young
    • 한국노년학
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    • v.40 no.4
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    • pp.781-800
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    • 2020
  • This study aims to categorize the daily time use of older adults into different types, examine the factors influencing them and the differences between them by type, and verify the effect of each type on life satisfaction. From the 6th wave of the Korea Retirement and Income Study (KeRIS), analysis was conducted on 3,963 older adults aged 65 (n=3,963). Using Latent Profile Analysis (LPA), three types of daily time use for older adults were identified: basic life maintenance type (16%), free-leisure oriented type (26%), and social activity type (58%). From the results of multinomial logistic regression analysis to identify the factors influencing each type of daily time use, it was found that the main factors influencing free-leisure oriented type, when compared with basic life maintenance type, were the presence of spouse (p<.01) and psychological health status (p<.001). The main factors influencing social activity type, when compared with basic life maintenance type, were age (p<.05), economic activity (p<.001), and chronic disease (p<.001). The life satisfaction seen during free-leisure oriented time and social activity time was higher than that during basic life maintenance time. From these findings, practical and policy proposals were suggested to improve life satisfaction of older adults during basic life maintenance time by pursuing changes in the use of that time.