• 제목/요약/키워드: Karp

검색결과 151건 처리시간 0.019초

대한방사선방어학회 회원수와 방어학회지에 게재된 년간 발표 논문 편수와의 관계 고찰 (Relation between KARP Membership and Articles Published in KARP Journal on the Yearly Basis)

  • 노성기;이재기
    • Journal of Radiation Protection and Research
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    • 제14권1호
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    • pp.71-74
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    • 1989
  • 최소자승법을 써서 우리 학회의 연차별 회원수와 학회지 게재 논문 편수와의 상관 관계식을 구하고, 그 결과로 부터 연간 4회까지 학회지를 발간코자 할 때의 필요 회원수를 추정한 바 최소한 650여명의 회원수를 확보해야만 할 것으로 보였다.

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Biochemical and Biophysical Characterizations of the Interaction between Two PDZ Adapter Proteins NHERF and E3KARP in vitro

  • Hwang, Eun-Young;Jeong, Mi-Suk;Jang, Se-Bok
    • Bulletin of the Korean Chemical Society
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    • 제31권11호
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    • pp.3241-3246
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    • 2010
  • NHERF ($Na^+/H^+$ exchanger regulatory factor) and E3KARP (NHE3 kinase A regulatory protein) play important roles in membrane targeting, trafficking and sorting of ion channels, transmembrane receptors and signaling proteins in many tissues. Each of these proteins contains two PDZ (PSD-95/Dlg-1/ZO-1) domains, which mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. The interaction between NHERF and E3KARP was investigated by surface plasmon resonance spectroscopy (BIAcore), fluorescence measurement, His-tagged pull-down experiment, and size-exclusion column (SEC) chromatography. BIAcore experiments revealed that NHERF bound to E3KARP with an apparent $K_D$ of 7 nM. Fluorescence emission spectra of the NHERF-E3KARP complex suggested that the tight interaction between these proteins was accompanied by significant conformational changes in one or both. The CD spectra of NHERF and E3KARP show that the conformational changes of these proteins were dependent on pH and temperature. These results implicate that the NHERF-E3KARP complex allows intracellular signaling complexes to form through PDZ-PDZ interactions.

Nested PCR을 이용한 사람 유래 태아 폐세포에서 배양된 Rickettsia tsutsugamushi의 혈청형 동정 (Use of Nested Polymerase Chain Reaction for Identification of Rickettsia tsutsugamushi Serotype Cultured in Human Embryonic Lung Cells)

  • 안창남;우규진;김태연;신광순;김철중;백락주
    • 대한바이러스학회지
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    • 제26권2호
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    • pp.235-244
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    • 1996
  • Rickettsia tsutsugamushi의 원형균주인 Karp주와 Gilliam주를 초대 배양된 사람 정상 2배체 폐세포(LuMA cell)를 이용하여 증식과 세포병변들의 속도를 비교할 수 있었고, 배양된 균주는 네스티드 프라이머를 사용하여 혈청형을 동정할 수 있었다. R. tsutsugamushi의 세포벽 외막에 존재하며 혈청형을 결정하는 주요항원은 54-56Kd 단백인 것으로 밝혀지고 있는데, 이 단백 유전자의 DNA 염기서열을 분석하여 Karp주와 Gilliam주의 공통서열로 첫번째 프라이머쌍을 만들었고 첫번째 프라이머쌍의 안쪽에 위치한 혈청형 사이에 차이가 있는 서열로 두번째 프라이머쌍을 만들었다. 네스티드 뉴클레오티드 프라이머는 중합효소 연쇄반응의 특이성을 증가시킬 수 있는데 이 실험 결과로 이 PCR 방법은 scrub typhus의 진단과 혈청형의 동정에 적용될 수 있을 것으로 보여진다.

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Longevity of Antibodies to Live Orientia tsutsugamushi Inoculated in Sprague Dawley Rats

  • An, Chang-Nam;Kim, Sung-Min;Kim, Tae-Yeon;Bae, Luck-Ju;Kim, Chul-Joong;Shin, Kwang-Soon;Park, Song-Yong
    • 대한바이러스학회지
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    • 제28권2호
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    • pp.193-201
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    • 1998
  • In Sprague Dawley (SD) rats, antibodies against strains of Orientia tsutsugamushi, Kato, Karp and Gilliam, were produced in order to investigate their longevity and cross-reactivities to their corresponding homologous and heterologous antigens. By immunofluorescence assay (IFA) of IgG and IgM, it was shown that the immunity to the homologous strains persisted at a higher level (longevity of at least 34 weeks with higher IFA titers). On the other hand, the immunity to the heterologous strains persisted at a lower level (longevity of 10 to 34 weeks with lower IFA titers). Since infection with one strain of O. tsutsugamushi does not preclude reinfection with other strains, understanding of the antigenic diversity of O. tsutsugamushi and duration of the immunity to both homologous and heterologous strain is very important in diagnosis of scrub typhus.

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한국산 다람쥐의 Orientia Tsutsugamushi에 대한 항체양성율 (Seropositive Rate of Orientia Tsutsugamushi in Tamias Sibiricus from Korea)

  • 송진원;이상원;고은영;정기모;이용주;송기준;백락주
    • 대한미생물학회지
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    • 제35권1호
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    • pp.61-68
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    • 2000
  • Among wild chipmunks, Tamias sibiricus, captured in Kyunggi and Kangwon province in Korea, 1997, seropositivity for Orientia tsutsugamushi was determined. Serological test for Orientia tsutsugamushi infection was performed using indirect immunofluorescent antibody technique (IFA). Of 243 wild chipmunks, 61 against Gilliam strain and 64 against Karp strain of Orientia tsutsugamushi were IFA positive. Seropositivity against Gilliam strain was shown 33.3% in Kyunggi and 23.5% in Kangwon province, and against Karp strain was shown 33.3% and 25.4%, respectively.

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TLU형 FPGA를 위한 새로운 다출력 함수 기술 매핑 알고리즘 (New Technology Mapping Algorithm of Multiple-Output Functions for TLU-Type FPGAs)

  • 박장현;김보관
    • 한국정보처리학회논문지
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    • 제4권11호
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    • pp.2923-2930
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    • 1997
  • 본 논문에서는 최근에 관심을 모으고 있는Table Look- Up형의 FPGA를 위한 다출력 함수로직 합성 알고리즘에 대해 기술한다. 본 고에서 제안하는 TLU형 FPGA를 위한 다출력 함수 로직 합성 방법은 기능적 분해 방법을 사용하였으며, 이 방법을 이용한 2가지의 새로운 알고리즘을 설명한다. 첫번째는 한 출력에 적용한 Rofh-Karp 알고리즘을 다출력에 웅용할 수 있도록 확장하였으며, 두 번째는 분해과정에서 공통 분해 함수를 찾는 효과적 인 알고리즘을 제안한다. 기술 매핑의 최적화 대상은 CLB 개수를 고려했으며, 벤치마크 테스트를 통한 일반적인 회로에 적용성 검증, 기존 알고리즘과의 성능 비교 및 개선에 대해 연구하였다. 논리 설계 합성기 구성 과정에서 새로운 알고리즘을 구현하여 실험한 결과를 기존의 다출력 함수 분해 방법과 비교하면 CLB 의 개수, 네트 수 등 성능과 수행 시간에서 매우 만족할 만한 결과를 얻었다.

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JHPS-KARP-ARPS Joint Program for Commemoration of 2021 Bo Lindell Medal to Discuss the Future of Radiation Protection among Young Scientists and the Award Recipient, Dr. Ogino

  • Fujibuchi, Toshioh;Ogino, Haruyuki;Taek, Han Ki;Tani, Kotaro;Emes, Daniel
    • Journal of Radiation Protection and Research
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    • 제46권2호
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    • pp.80-82
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    • 2021
  • Dr. Haruyuki Ogino, a member of the Japan Health Physics Society (JHPS), was awarded the 2021 Bo Lindell Medal by the International Commission on Radiological Protection (ICRP). To commemorate this, the "Joint JHPS-KARP-ARPS program for young radiation protection (RP) scientists to discuss the future of RP" was organized via a web meeting system. First, Dr. Ogino gave a lecture, and then young researchers selected from each academic society made presentations on the future of RP. After the presentations by the three researchers, a free talk was held-young researcher groups of each country being active is a great opportunity to collaborate and exchange information. It was emphasized that the low awareness of knowledge related to radiation has been common to all of the participating countries. Thus, it is necessary to utilize communication via web technology, as done for this program, effectively. One of the biggest advantages for Asia and Oceania is that we do not have a significant time difference. The round-table discussion was concluded by expressing the hope of active exchange and development of young researchers in the future.