• Title/Summary/Keyword: Kagawa

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Effect of Aminoglycoside Antibiotics on in-Vitro Morphogenesis from Cultured Cells of Chrysanthemum and Tobacco

  • Teixeira da Silva, Jaime A.;Fukai, Seiichi
    • Journal of Plant Biotechnology
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    • v.6 no.1
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    • pp.25-37
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    • 2004
  • Successful genetic transformation of plants requires non-chimeric selection of transformed tissues and their subsequent regeneration. With rare exceptions, most transformation protocols still rely heavily on antibiotics for selecting transgenic cells that contain an antibiotic-degrading selectable marker gene. Here, the morphogenic capacity of in-vitro explants of chrysanthemnum and tobacco stems and leaves (control and transgenic) changed with the addition of aminoglycoside antibiotics (AAs), In a test of 6 AAs, phytotoxicity occurred at concentrations of 10 to 25 and 50 to 100$\mu\textrm{g}$ $mL^{-1}$ in chrysanthemum and tobacco explants, respectively. Light conditions as well as explant source and size also had significant effects. The use of transverse thin cell layers (tTCLs), in conjunction with high initial AA selection levels, supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow-cytometric analyses revealed no endodu-plication in chrysanthemum, even at high AA levels. However, this phenomenon was observed in tobacco calli(8C or more), even at low AA concentrations (i.e., 5 to 10 $\mu\textrm{g}$ mL$^{-1}$ ).

Automatic Vowel Sequence Reproduction for a Talking Robot Based on PARCOR Coefficient Template Matching

  • Vo, Nhu Thanh;Sawada, Hideyuki
    • IEIE Transactions on Smart Processing and Computing
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    • v.5 no.3
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    • pp.215-221
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    • 2016
  • This paper describes an automatic vowel sequence reproduction system for a talking robot built to reproduce the human voice based on the working behavior of the human articulatory system. A sound analysis system is developed to record a sentence spoken by a human (mainly vowel sequences in the Japanese language) and to then analyze that sentence to give the correct command packet so the talking robot can repeat it. An algorithm based on a short-time energy method is developed to separate and count sound phonemes. A matching template using partial correlation coefficients (PARCOR) is applied to detect a voice in the talking robot's database similar to the spoken voice. Combining the sound separation and counting the result with the detection of vowels in human speech, the talking robot can reproduce a vowel sequence similar to the one spoken by the human. Two tests to verify the working behavior of the robot are performed. The results of the tests indicate that the robot can repeat a sequence of vowels spoken by a human with an average success rate of more than 60%.

Dynamic Analysis of Lateral Pile under Seismic Loading (지진하중을 받는 파일기초의 횡방향 동적 거동해석)

  • 이인모;이현종
    • Geotechnical Engineering
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    • v.4 no.1
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    • pp.29-36
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    • 1988
  • Dynamic analysis of lateral pile under seismic loading is performed in this paper. As an analytical model, the Bean-on-twinkler Foundation Model is used for this study because of its simplicity and acceptible accuracy . The method suggested by Kagawa and Kraft, which can account for non-linear effects, is used for the dynamic P-y relationship This relationship is found to be the most important factor in analysis . Group pile effects are also considered approximately The results of dynamic analysis show that a pile without supporting mass follows the soil movement ; in the case of a pile with supporting mass, the relative displacement between the soil and the pile occurs . When designing piles, it must be considered that piles have to resist the curvatures originated by the soil movement.

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A mixture of Salacia oblonga extract and IP-PA1 reduces fasting plasma glucose (FPG) and low-density lipoprotein (LDL) cholesterol levels

  • Nakata, Kazue;Taniguchi, Yoshie;Yoshioka, Noriko;Yoshida, Aya;Inagawa, Hiroyuki;Nakamoto, Takeru;Yoshimura, Hiroshi;Miyake, Shin-Ichiro;Kohchi, Chie;Kuroki, Masahide;Soma, Gen-Ichiro
    • Nutrition Research and Practice
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    • v.5 no.5
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    • pp.435-442
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    • 2011
  • At present, lifestyle-related diseases are one of the most critical health issues worldwide. It has been reported that lipopolysaccharide derived from a Gram-negative bacteria (IP-PA1) symbiotic with wheat exhibited several advantageous biological effects, such as the reduction of plasma glucose levels in NOD mice and low-density lipoprotein (LDL) levels in WHHL rabbits. In this study, the beneficial effects on plasma glucose and lipids of a tea (SI tea) consisting of IP-PA1 and Salacia (which contains an inhibitor of ${\alpha}$-glucosidase) were investigated in the KK-Ay/TaJcl type 2 diabetic model mice and in human subjects with premetabolic syndrome in a double-blind, randomized study. S1 tea significantly decreased plasma glucose levels in KK-Ay/TaJcl mice. A clinical trial of SI tea was performed with 41 subjects between the ages of 40 and 69, who belonged either to a high plasma glucose group (HG: FPG 100-125 mg/dl) or to a hyperlipidemia group (HL: TG ${\geq}$ 150 mg/dl, or LDL ${\geq}$ 120 mg/dl, or HDL <40 mg/dl). These subjects ingested either Salacia without IP-PA1 (the control) or SI tea. Blood samples were collected at 0, 30, and 60 days after initiating SI tea treatment, and were measured for FPG, HbA1c, TG, LDL, and HDL. These results showed that SI tea reduced FPG and HbA1c more rapidly than the control in the HL group, and also significantly improved LDL and HDL levels in the HG group. Thus, SI tea may be helpful in preventing lifestyle-related diseases.

Modeling and designing a power assist circuit using artificial muscle

  • Kagawa, Toshiharu;Fujita, Toshinori;Kawashima, Kenji
    • 제어로봇시스템학회:학술대회논문집
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    • 1993.10b
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    • pp.121-126
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    • 1993
  • Artificial muscle actuators are used in various fields. Especially, they are applied to the power assist circuit to make use of their characteristics. The purpose of this paper is to and analyze the power assist circuit using an artificial muscle actuator. As a result, it is found that the operating feeling of the power assist circuit depends mainly on the flow gain of the pneumatic servo valve. The required flow gain is calculated from the proposed model, and the experimental results agreed with the calculated results.

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Fault diagnosis based on likelihood decomposition

  • Uosaki, Katsuji;Kagawa, Tetsuo
    • 제어로봇시스템학회:학술대회논문집
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    • 1992.10b
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    • pp.272-275
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    • 1992
  • A novel fault diagnosis method based on likelihood decomposition is proposed for linear stochastic systems described by autoregressive (AR) model. Assuming that at some time instant .tau. the fault of one of the following two types is occurs: innovation fault (actuator fault); and observation fault (sensor fault), the log-likelihood function is decomposed into two components based on the observations before and after .tau., respectively, Then, the type of the fault is determined by comparing the log-likelihoods corresponding two types of faults. Numerical examples demonstrate the usefulness of the proposed diagnosis method.

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Oscillatory modes generated by Hopf bifurcations in coupled four oscillators

  • Kitajima, Hiroyuki;Kawakami, Hiroshi
    • Proceedings of the IEEK Conference
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    • 2002.07c
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    • pp.1634-1637
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    • 2002
  • We examine the oscillatory modes generated by the Hopf bifurcations of non-origin equilibrium points in the four-coupled oscillator system. The Hopf bifurcations of the equilibrium points and the generated oscillatory modes are classified. By numerical bifurcation analysis we observe various interesting synchronized states caused by symmetry-breaking bifurcations.

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