• The Korean Journal of Physiology and Pharmacology
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• v.20 no.2
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• pp.193-200
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• 2016

Sertraline, a selective serotonin reuptake inhibitor (SSRI), has been reported to lead to cardiac toxicity even at therapeutic doses including sudden cardiac death and ventricular arrhythmia. And in a SSRI-independent manner, sertraline has been known to inhibit various voltage-dependent channels, which play an important role in regulation of cardiovascular system. In the present study, we investigated the action of sertraline on Kv1.5, which is one of cardiac ion channels. The effect of sertraline on the cloned neuronal rat Kv1.5 channels stably expressed in Chinese hamster ovary cells was investigated using the whole-cell patch-clamp technique. Sertraline reduced Kv1.5 whole-cell currents in a reversible concentration-dependent manner, with an $IC_{50}$ value and a Hill coefficient of $0.71{\mu}M$ and 1.29, respectively. Sertraline accelerated the decay rate of inactivation of Kv1.5 currents without modifying the kinetics of current activation. The inhibition increased steeply between -20 and 0 mV, which corresponded with the voltage range for channel opening. In the voltage range positive to +10 mV, inhibition displayed a weak voltage dependence, consistent with an electrical distance ${\delta}$ of 0.16. Sertraline slowed the deactivation time course, resulting in a tail crossover phenomenon when the tail currents, recorded in the presence and absence of sertraline, were superimposed. Inhibition of Kv1.5 by sertraline was use-dependent. The present results suggest that sertraline acts on Kv1.5 currents as an open-channel blocker.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.6
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• pp.545-553
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• 2020

Aripiprazole is a quinolinone derivative approved as an atypical antipsychotic drug for the treatment of schizophrenia and bipolar disorder. It acts as with partial agonist activities at the dopamine D2 receptors. Although it is known to be relatively safe for patients with cardiac ailments, less is known about the effect of aripiprazole on voltage-gated ion channels such as transient A-type K⁺ channels, which are important for the repolarization of cardiac and neuronal action potentials. Here, we investigated the effects of aripiprazole on Kv1.4 currents expressed in HEK293 cells using a whole-cell patch-clamp technique. Aripiprazole blocked Kv1.4 channels in a concentration-dependent manner with an IC₅₀ value of 4.4 μM and a Hill coefficient of 2.5. Aripiprazole also accelerated the activation (time-to-peak) and inactivation kinetics. Aripiprazole induced a voltage-dependent (δ = 0.17) inhibition, which was use-dependent with successive pulses on Kv1.4 currents without altering the time course of recovery from inactivation. Dehydroaripiprazole, an active metabolite of aripiprazole, inhibited Kv1.4 with an IC₅₀ value of 6.3 μM (p < 0.05 compared with aripiprazole) with a Hill coefficient of 2.0. Furthermore, aripiprazole inhibited Kv4.3 currents to a similar extent in a concentration-dependent manner with an IC₅₀ value of 4.9 μM and a Hill coefficient of 2.3. Thus, our results indicate that aripiprazole blocked Kv1.4 by preferentially binding to the open state of the channels.

• 전기의세계
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• v.16 no.5
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• pp.51-62
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• 1967

Relay Protector의 자체특성시험을 한 다음 대전-이리, 대구-상주, 대구-진영 간의 154KV 송전선과 이를 인접역행하는 통신선에 Relay Protector를 취부하고 이 구간의 154KV 송전선을 직접접지한 후에 실제로 인공고장을 이르켜 Relay Protector의 성능실험을 하였다.

• 전기의세계
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• v.17 no.3
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• pp.57-61
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• 1968

Limitation to Peterson coil grounded operation of 154KV system began to develop with the growth of system as to voltage and system cost. Therefore, 154KV system is going to be grounded solidly from November this year. This study shows the insulation coordination of solidly ground system, such as the selection of a suitable lightening arrester, determination of transmission line insulation and Basic Insulation Level of station equipment.

• 전기의세계
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• v.15 no.4
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• pp.27-38
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• 1966

본 고에서는 앞으로 신설된 배전계통에서의 고저압혼독시 전위상승에 대해 알아보고자 한다. A항의 검토에서 고목계통의 실측치와 D.C. board에 의한 계산치 사이에 약 10-20%의 오차는 있으나 계산치로써 전위상승을 추정할 수 있음을 알았음으로 신설될 23kv Y및 11.4kv Y배전방식에 대하여 계산한다.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.32 no.3
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• pp.84-89
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• 1983

In this paper, theoretical equations have been obtained on scattering of television waves fromthe multi-conductor of 345KV power lines. Intensities of the scattered fields are calculated for a single conductor,

• 전기의세계
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• v.25 no.1
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• pp.61-63
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• 1976

전력수요가 증가하고 전원개발의 진보에 따라 전력의 수송거리가 길어지고 수송전력은 커지므로써 경제적인 송전전압은 높아져서 세계 각국에서는 초고압 송전이 증가하고 있다. 우리나라에서도 345KV계통이 건설되어 아직은 154KV운전중이나 머지않아 345KV로 운전되므로써 전력격통에 크게 공헌할 것으로 기대되고 있다. 이에 세계주요국의 초고압송전의 현황과 전망에 대하여 살펴보기로 한다.

• Korean Journal of Animal Reproduction
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• v.27 no.1
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• pp.87-93
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• 2003

This study was conducted to investigate the effects of embryo development by fusion condition on the nuclear transfer with brindle coated cow's ear cells. Ear cells were transferred into an enucleated oocyte and fused with cytoplasm in the fusion condition with 1.9kv/cm, 2.0kv/cm, 2.1kv/cm each 10 and 20ug duration Nuclear transfer embryo were activeted with a combination of 5ug/ml and 1.9mM 6-DMAP (4min, 4h). Fusion rate was 51∼68% range among fusion condition (1.9, 2.0, 2.1kv/cm; 10, 20us). But, cytoplasm lysis rate was increased by higher electric condition (0∼51.8% range). Each parameter's cleavage and blastocyst formation rate were 1.9kv/cm for 10us (75.8 and 19.5%), 20us (69.8 and 48.6%), 2.0kv/cm for 10us (76.9 and 20.0%), 20 us (68.5 and 40.9%), 2.1kv/cm for 10us (70.5 and 44.2%), 20 us (68.5 and 27.0%). We compared the effectiveness of cloning for between brindle coated cow's ear cells and Hanwoo fetal fibroblast cells. There was no significant differences in the fusion rate and developmental rate to the blastocyst stage. After transfer of blastocysts derived from nuclear transfer embryos, pregnancy rates of the Hanwoo fetal fibroblast cells and brindle coated cow's ear cells were checked pregnant on day 60 as assessed by ultrasonography, 40% (2/5) and 15.8% (3/19), respectively. This studies conclude that brindle coated cow's ear cells have the developmental potentiality to term by nuclear transfer. These results demonstrate that the increased the field strength was to be profitable for development of blastocyst or reduce of cytoplasm's damage than increasing the pulse duration.

• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.125-132
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• 2002

This study was conducted to examine the effects of electric stimulation conditions on in vitro developmental ability of procine embryos after somatic cell nuclear transfer, The porcine ear cell was cultured in vifro for confluency in serum-starvation condition (TCM-199＋0.5% FBS) for cell confluency. The zona pellucida of IVM oocytes were partially drilled using laser system. Single somatic cell was individually transferred into the enucleated oocyte. The reconstructed embryos were electrically fused with 0.3M mannitol. After electric fusion, the embryos were activated and cultured in NCSU-23 medium containing 10% FBS at 39$^{\circ}C$, 5% $CO_2$ in air for 6 to 8 days. Nuclear transferred(NT) oocytes which fused at a field strength of 1.90kv/cm showed a higher (P<0.05) fusion rate(49.5%, 50/101) compared to 2.10 kv/cm(25.8%, 24/93) or 2.50kv/cm(30.3%, 27/89). After electric activation, the cleavage rate of NT embryos was 48.0(24/50), 66.6(16/24) and 70.3% (19/27), respectively and these were not different. There was no significant difference in fusion rate by duration and pulse of electric stimulation. In cleavage rate, however, more NT embryos(76.3%, 45/59) cleaved at 60 $\mu$sec twice than other embryos(49.1 to 56.5%) with different conditions of electric stimulation(P<0.05). NT embryos activated at a field strength of 1.50kv/cm showed a higher developmental rate(9.8%, 5/51) than those embryos activated at 1.25kv/cm(0%) or parthenotes(6.4%, 7/109). These results suggest that some factors such as field strength, duration and pulse of electric stimulation could be affected to in vitro developmental ability of nuclear transplanted porcine embryos.

• 전기의세계
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• v.28 no.10
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• pp.9-16
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• 1979

근래 우리나라의 국민소득이 높아짐에 따라 가전제품의 수요가 늘고, 중화학공업의 발달에 따라 전력수요가 급격히 증가하고 있다. 이에 반하여 석유가는 매년 증가하고 있으므로 기력발전소를 지양하고 대용량의 원자력, 양수발전소, 석탄발전소가 건설되고 있는 경향이 있다. 발전소는 입지조건상 해안 또는 강변에 위치하고 있으며 수요지와는 거리가 멀고 또는 계통의 안전도(Stability), 신뢰도(Reliability)를 높이기 위하여 154KV와 345KV 송전선으로 전계통을 연계하고 있다. 송전선의 전력운송능력을 증대시키기 위하여 복도체 또는 사도체로 하고 있으며 전선도 ACSR에서 TACSR로 하는 나라도 있다. 현재 미국에서는 765KV Canada에서는 735KV 송전선으로 운전되고 있으며 장차 1500KV 송전선도 곧 실용화된다고 한다. 이에 따라 송전선의 지지물인 철탑도 대형화되어가고 높이도 증가하게 되어 이의 제작및 건설비가 막대하게 들게되며 앞으로는 용지확보도 더욱 어렵게 되어 송전선의 회전수를 2 또는 4회선으로 건설하여야 될것이다. 송전선철탑설계는 종래에는 Cremona도식해법에 의하여 설계하였으나 이의 작도및 하중조건이 많아지면 부재의 정확한 설계하중을 산출하는 데는 많은 시간과 노력이 필요하다. 이러한 불편을 헤소하기 위하여 Ritter의 수치해석법(moment 방법)을 이용한 전산화 및 설계이론에 대하여 소개하고자 한다.