• 한국식품저장유통학회지
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• 제6권2호
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• pp.194-199
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• 1999

한국품종 및 중국품종 참깨를 한국 조치원 및 중국 길림성에서 각각 재배, 수확한 참깨 및 이를 볶아 제조한 참기름을 시료로 하여 화학성분을 분석 비교하였다. 참기름의 수율은 한국품종 한국산이 47.8%, 중국품종 중국산이 48.1%, 재배지 교환시는 한국품종 중국산이 48.6%, 중국품종 한국산이 49.3%로 나타났다. 참기름의 색도는 한국품종 한국산이 L값이 다소 낮아 참기름 고유의 암갈색을 띠었다. 참기름의 지방질 조성은 한국품종 한국산은 중성, 당, 인지질의 조성비가 93.1%, 6.6%, 0.2%였고, 중국품종 중국산은 94.7%, 4.6%, 0.8%로 조성면에서 큰 차이는 없었으며, 재배지를 교환시도 비슷한 경향을 보였다. 참기름의 지방산 조성은 모두 7개의 지방산을 확인하였으며, 불포화 지방산인 oleic acid 및 linoleic acid가 한국품종 한국산이 84.6%, 중국품종 중국산이 84.8%로 대부분을 차지하였고, 그 조성면에서는 oleic acid 및 linoleic acid가 한국품종 한국산은 47.1%, 37.5%인데 비해 중국품종 중국산은 40.5%, 44.3%로 다소 차이를 보였다. 참깨의 아미노산 함량은 생시료의 경우 한국품종 한국산이 713mg%로서 중국품종 중국산 238mg%보다 월등히 높았으며, 필수 아미노산 함량 또한 한국품종 한국산이 309mg%로서 중국품종 중국산 99mg%에 비해 월등히 높게 나타났다. 한편 재배지를 교환시 아미노산 함량은 한국품종 중국산은 한국 재배시보다 44% 정도 감소하였고 중국품종 한국산은 중국재배시 보다 63% 정도 증가하였으며, 시료를 볶음처리했을 경우 아미노산 함량은 한국산 및 중국산 모두 현저한 감소를 보였다.

• Archives of Pharmacal Research
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• 제29권10호
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• pp.834-839
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• 2006

Torilin was purified from Torilis japonica (Houtt.) DC., and its effects on a rapidly activating delayed rectifier $K^+$ channel (hKv1.5), cloned from human heart and stably expressed in Ltk cells, as well as the corresponding $K^+$ current (the ultrarapid delayed rectifier, $I_{KUR}$) were assessed in human atrial myocytes. Using the whole cell configuration of the patch-clamp technique, torilin was found to inhibit the hKv1.5 current in time and voltage-dependent manners, with an $IC_50$ value of $2.51{\pm}0.34\;{\mu}M$ at +60 mV. Torilin accelerated the inactivation kinetics of the hKv1.5 channel, and slowed the deactivation kinetics of the hKv1.5 current, resulting in a tail crossover phenomenon. Additionally, torilin inhibited the hKv1.5 current in a use dependent manner. These results strongly suggest that torilin is a type of open-channel blocker of the hKv1.5 channel.

• The Korean Journal of Physiology and Pharmacology
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• 제26권2호
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• pp.135-144
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• 2022

An antidiabetic drug, rosiglitazone is a member of the drug class of thiazolidinedione. Although restrictions on use due to the possibility of heart toxicity have been removed, it is still a drug that is concerned about side effects on the heart. We here examined, using Chinese hamster ovary cells, the action of rosiglitazone on Kv1.5 channels, which is a major determinant of the duration of cardiac action potential. Rosiglitazone rapidly and reversibly inhibited Kv1.5 currents in a concentrationdependent manner (IC₅₀ = 18.9 μM) and accelerated the decay of Kv1.5 currents without modifying the activation kinetics. In addition, the deactivation of Kv1.5 current, assayed with tail current, was slowed by the drug. All of the results as well as the usedependence of the rosiglitazone-mediated blockade indicate that rosiglitazone acts on Kv1.5 channels as an open channel blocker. This study suggests that the cardiac side effects of rosiglitazone might be mediated in part by suppression of Kv1.5 channels, and therefore, raises a concern of using the drug for diabetic therapeutics.

• The Korean Journal of Physiology and Pharmacology
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• 제27권1호
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• pp.95-103
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• 2023

Rosiglitazone is a thiazolidinedione-class antidiabetic drug that reduces blood glucose and glycated hemoglobin levels. We here investigated the interaction of rosiglitazone with Kv3.1 expressed in Chinese hamster ovary cells using the wholecell patch-clamp technique. Rosiglitazone rapidly and reversibly inhibited Kv3.1 currents in a concentration-dependent manner (IC₅₀ = 29.8 µM) and accelerated the decay of Kv3.1 currents without modifying the activation kinetics. The rosiglitazonemediated inhibition of Kv3.1 channels increased steeply in a sigmoidal pattern over the voltage range of -20 to +30 mV, whereas it was voltage-independent in the voltage range above +30 mV, where the channels were fully activated. The deactivation of Kv3.1 current, measured along with tail currents, was also slowed by the drug. In addition, the steady-state inactivation curve of Kv3.1 by rosiglitazone shifts to a negative potential without significant change in the slope value. All the results with the use dependence of the rosiglitazone-mediated blockade suggest that rosiglitazone acts on Kv3.1 channels as an open channel blocker.

• 대한수의학회지
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• 제48권4호
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• pp.423-429
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• 2008

Throughout the world, rotavirus infections cause extensive morbidity in human infants and diarrhea in animals such as white scour caused by bovine rotavirus in calves. We isolated three rotavirus strains designated KV0407, KV0418, and KV0426 from 103 fecal samples of diarrheic calves. The genes coding for proteins VP4, VP6, VP7, and NSP4 from strain KV0407 were sequenced and compared with the nucleotide sequences of other known strains of rotavirus. The KV0407 VP4 gene was highly homologous to the OSU (99.4%) and JL94 (99.4%), but not the B223 (62.4%) and K33 (62.4%) VP4 genes. The KV0407 and KV0418 VP7 genes were most similar to the OSU and super-short type VMRI VP7 genes. Based on nucleotide sequence analysis, the KV0407 strain was tentatively assigned to A serogroup (SG I), G5P[7], NSP4 genotype B and the KV0418 and KV0426 strains were assigned to A serogroup (SG I), G6P[5], NSP4 genotype A. The genetic characterization of these bovine rotavirus isolates could be useful for the diagnosis and prevention of diarrhea in calves.

• Clinical and Experimental Reproductive Medicine
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• 제18권1호
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• pp.35-40
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• 1991

To find out the suitable method for blastomeres fusion of mouse 2-cell embryo using electric stimuli, these studies were carried out with various voltages (1.0 KV, 1.2 KV, 1.5 KV, 1.7 KV and 2.0KV), pulse duration times($50{\mu}\;sec$, $75/{\mu}\;sec$, $100{\mu}\;sec$) and different fusion solutions. In addition, the fused embryos were cultured for 72-80hr to observe their subsequent development. These results were summarized as follows: 1. The proportion of the fused embryos were 50.8%(34/67), 60.7%(34/56), 70.6%(48/68), 66.7% (48/72) and 85.3% (58/68) after stimuli of 1.0KV, 1.2KV, 1.5KV, 1.7KV and 2.0KV for $100{\mu}\;sec$ with 2 times, and the electric stimulation at 2.0KV(85.3%) was the most effective voltage on the blastomere fusion. 2. For in vitro development, blastocysts of the fused embryos were cultured for 72-80hrs in $M_{16}$ medium. The group(52.1%) treated with 1.5KV for $100{\mu}\;sec$ with 2 times showd higher development rates than those any other group. However, these results were not corresponded to those of the rates of blastomere fusion. 3. There were no significant differences among the rates of blastomeres fusion to 50(70.6%), 75(71.9%), and 100(78.0%) ${\mu}sec$ stimulation at 1.5KV with two times. However, the development rates of the fused embryo in vitro were 52.1%(25/48), 28.3%(13/46) and 9.4%(3/32) at the above conditions, and the development rates of fused embryo increased as the pulse duration times increased. 4. The rates of the blastomeres fusion were 38.9% (28/72) or 70.6% (48/68) in electrolyte (PBS) or non-electrolyte(0.3M mannitol) solution. The development rates of the fused embryo were 32.1% (9/28) or 52.1%(25/48) in the above fusion solutions, and non-electrolyte-treated group showed higher development rates of embryo than that of electrolyte-treated group.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1992년도 하계학술대회 논문집 B
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• pp.903-906
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• 1992

At present the XLPE insulated power Cables have installed until 154KV level in KOREA. Therefore, our company has developed 275KV XLPE cable as a means 345KV level-up of CV cable as well as exploitation of overseas markets. In this paper, the outline of design standards, manufacturing processes and test performance of 275KV CV cable are described.

• 한국안전학회지
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• 제8권3호
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• pp.34-43
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• 1993

This paper presents a study on the characteristics and safety criteria for human body in ELF (Extremely Low Frequency : 50-60Hz) electric and magnetic fields. Many researches for ELF electric and magnetic fields, which are developed in the past, are studied and analyzed In this paper. In order to estabilish the safety criteria for human body in the field, the field intensity, induced current and voltage are calculated by the electrostatic field approach which is far simpler than the electromagnetic field one based on Maxwell equation. The method is applied to the 345 KV transmission line system In operation and 765 KV system under consideration. According to the results, the maximum value of field intensity, 6.8627KV/m, is evaluated at the location which is 14m away from transmission line. As the safety criteria value by the abroad researches asserting that the human can detect the Induced current in 6KV/m and above, 5KV/m and 7KV/m are recommended at residence area and nonresidence area, respectively.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1988년도 전기.전자공학 학술대회 논문집
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• pp.774-777
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• 1988

This paper covers the insulation coordination of 765 KV transmission line in Korea Electric Power Corporation. The design for the lightning, switching surge, and contamination was conducted to increase the reliability of 765 KV line. The authors have used the result of a limited Westinghouse ANACOM swiching surge study of the Korea Electric Corporation's 765 KV Expansion plan. The study investigated the switching surge overvoltage distribution and maximum switching overvoltage when relosing 765 KV lines. They used the technical data of the suspension insulator for the contamination design, which was available from a foreign insulator manufacture. From this paper, the authors find out that the contamination design dominates to determine the number of insulator and the SOV design dominates the air clearance in the tower. Afterwards the authors will confirm this design by Demonstration Test with Korea Electro-technology Research Institute.

• The Korean Journal of Physiology and Pharmacology
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• 제21권4호
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• pp.415-421
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• 2017

We investigated the inhibitory effect of escitalopram, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent $K^+$ (Kv) channels in freshly separated from rabbit coronary arterial smooth muscle cells. The application of escitalopram rapidly inhibited vascular Kv channels. Kv currents were progressively inhibited by an increase in the concentrations of escitalopram, suggesting that escitalopram inhibited vascular Kv currents in a concentration-dependent manner. The $IC_{50}$ value and Hill coefficient for escitalopram-induced inhibition of Kv channels were $9.54{\pm}1.33{\mu}M$ and $0.75{\pm}0.10$, respectively. Addition of escitalopram did not alter the steady-state activation and inactivation curves, suggesting that the voltage sensors of the channels were not affected. Pretreatment with inhibitors of Kv1.5 and/or Kv2.1 did not affect the inhibitory action of escitalopram on vascular Kv channels. From these results, we concluded that escitalopram decreased the vascular Kv current in a concentration-dependent manner, independent of serotonin reuptake inhibition.