• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.337-345
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• 2014

Pichia pastoris is one of the most widely used expression systems for the secretory expression of recombinant proteins. The secretory expression in P. pastoris usually makes use of the prepro $MAT{\alpha}$ sequence from Saccharomyces cerevisiae, which has a dibasic amino acid cleavage site at the end of the signal sequence. This is efficiently processed by Kex2 protease, resulting in the secretion of high levels of proteins to the medium. However, the proteins that are having the internal accessible dibasic amino acids such as KR and RR in the coding region cannot be expressed using this signal sequence, as the protein will be fragmented. We have identified a new signal sequence of 18 amino acids from a P. pastoris protein that can secrete proteins to the medium efficiently. The PMT1-gene-inactivated P. pastoris strain secretes a ~30 kDa protein into the extracellular medium. We have identified this protein by determining its N-terminal amino acid sequence. The protein secreted has four DDDK concatameric internal repeats. This protein was not secreted in the wild-type P. pastoris under normal culture conditions. We show that the 18-amino-acid signal peptide at the N-terminal of this protein is useful for secretion of heterologous proteins in Pichia.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.1
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• pp.97-103
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• 2003

Numerical research has been done for the transverse jet behind a rearward-facings step in turbulent supersonic flow without chemical reaction. Purpose of transverse jet is to enhance mixing of the fuel in the combustor. Two-dimensional unsteady flowfields generated by slot injection into supersonic flow are numerically simulated with the Navier-Stokes equations with two-equation k-$\varepsilon$ turbulence model. Numerical method is used high-order upwind TVD scheme. Eight cases are computed for different slot momentum flux ratios and slot position at downstream of the step. The flow is very similar to the cavity flow, because the jet acts as an obstacle. The numerical results thus show the periodic phenomenon.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.1
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• pp.71-81
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• 2011

Due to increased human activities and intensive rainfall events in a watershed, soil erosion and sediment transport have been hot issues in many areas of the world. To evaluate soil erosion problems spatially and temporarily, many computer models have been developed and evaluated over the years. However, it would not be reasonable to apply the model to a watershed if topography and environment are different to some degrees. Also, source codes of these models are not always public for modification. The ArcGIS model builder provides ease-of-use interface to develop model by linking several processes and input/output data together. In addition, it would be much easier to modify/enhance the model developed by others. Thus, simple model was developed to decide soil erosion hot spot areas using ArcGIS model builder tool in this study. This tool was applied to a watershed to evaluate model performance. It was found that sediment yield was estimated to be 13.7 ton/ha/yr at the most severe soil erosion hot spot area in the study watershed. As shown in this study, the ArcGIS model builder is an efficient tool to develop simple models without professional programming abilities. The model, developed in this study, is available at http://www.EnvSys.co.kr/~sateec/toolbox for free download. This tool can be easily modified for further enhancement with simple operations within ArcGIS model builder interface. Although very simple soil erosion and sediment yield were developed using model builder and applied to study watershed for soil erosion hot spot area in this study. The approaches shown in this study provides insights for model development and code sharing for the researchers in the related areas.

• Korean Institute of Interior Design Journal
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• v.25 no.2
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• pp.123-130
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• 2016

Industrialization and Urbanization caused a great influx of rural population into the cities, which caused housing types to change from detached homes to more apartments homes. The official 2014's Statistics (http://kossir.kr) show that half (49.6%) of the Korean population live in apartment buildings and 37.5% live in detached houses. For this reason, the apartments have become the typical housing in Korea. So, the aim of this study is to compare and analyze the internal apartment space of Budang (New-Town) and Wirye (New-Town), since these Budang apartments are the first new-town housing and Wirye apartments are the last new-town housing in Korea. I have used the Convex Analysis of Space Syntax Program in analyzing the inside space of both of these locations' apartments. The results are as follows : First, the inside space of Wirye apartments have more convex space than that of the Budang apartments. Second, the common inside space of the Budang apartments were planned by integration space, on the other hand the inside space of Wirye apartments were planned by segregation space. Third, the master bedroom in both of the locations' apartments were analyzed for their strong integration space which they may have in common. The K (Kitchen) + D (Dining) space in Budang apartments are classified as integration space, on the other hand L (Living) + K + D space is also classified as integration space. The L+K+D space of the Wirye apartments is classified as integration space. Finally, the inside space of the Wirye apartments were planned more for various spaces than that of Budang apartments. The concept of master bedroom in both new-town was not planed for a couple privacy space but the heart of the family.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.12
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• pp.10-22
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• 2009

This paper presents an LED lighting system with an LED color control algorithm that can independently change its color temperature and illuminance. To show the validity of the proposed algorithm, it is proven that its solution always exists. The proposed algorithm was applied to the control of an LED module that is composed of red, green, blue, and white (RGBW) LEDs. Its color temperature variation ranged from 3,500~7,500[$^{\circ}K$], and its illuminance ranges from 500~1,500[lux]. Within these range, the color temperature and illuminance deviations are as low as $\pm0.8$[%] when the junction temperature of LEDs are maintained at 40[$^{\circ}C$]. In the range of 30~70[$^{\circ}C$], the measured illuminance and color temperature deviations are as low as 2.1[%] and 3.6[%], and the compensated ones are as low as 1[%] and 0.49[%], when the desired illuminance and color temperature are 1,000[lux] and 6,500[$^{\circ}K$], respectively.nyang.ac.kr).

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.2
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• pp.40-48
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• 2016

To observe the rail-slab interaction in continuous welded railway(CWR) bridge when earthquake occurs, additional axial rail stresses and relative longitudinal displacements between rail and bridge deck were calculated with input of various load combinations and 3 different types of seismic loads to an analytical model. As results of analysis, it can be found that standard response spectrum proposed by Korea Rail(KR) network authority for earthquake design showed less additional axial rail stresses than allowable levels, but greater relative longitudinal displacement between rail and bridge deck, which means that adjustment of relative longitudinal displacement within a standard level is much more difficult than axial train stress. Additionally, if a large-scaled earthquake as occurred at Kobe, Japan comes up, then both of additional axial rail stress and relative displacement in rail-bridge deck may exceed allowable levels, which indicates to make proper design guides against sudden earthquake occurrence.

• Resources Recycling
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• v.18 no.1
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• pp.58-67
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• 2009

The annual requirement of domestic waste photoresist stripper solution is about 1 trillion won level (50,000 tons) depends entirely on imports for its supplies. Nevertheless, there waste strippers which contain the impurities have been recycled or incinerated to a low level of recycle. These days the recycling technologies of the waste stripper solution has been widely studied from the view points of economy and efficiency. In this paper, the recycling technologies on patent of the waste stripper solution were analyzed. The range of search was limited within the open patents of USA (US), European Union (EP), Japan (JP), and Korea (KR) up to september, 2007. Patents have been collected using key-words searching and filtered by filtering criteria. The trends of the patents was analyzed by the years, countries, companies, and technologies.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.06a
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• pp.19-19
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• 2007

Excimer laser annealing (ELA) 방법을 이용하여 결정화하고 게이트 절연체로써 high-k 물질을 가지는 다결정 실리콘박막 트랜지스터의 전기적 특성을 평가하였다. 다결정 실리콘 박막 트랜지스터는 비결정질 실리콘 박막 트랜지스터 보다 높은 전계 효과 이동도와 운전 용이한 장점을 가진다. 기존의 결정화 방법으로는 다결정 실리콘 박막 트랜지스터의 높은 열 공급을 피할 수 없기 때문에, 매몰 산화막 위의 비결정질 박막은 저온에서 다결정 실리콘 결정화를 위해 KrF excimer laser (248nm)를 이용하여 가열 냉각 공정을 했다. 게다가 케이트 절연체로써 atomic layer deposition (ALD) 방법에 의해 저온에서 20 nm의 고 유전율을 가지는 $HfO_2$ 박막을 증착하였다. 알루미늄은 n-MOS 박막 트랜지스터의 게이트 전극으로 사용되었다. 금속 케이트 전극을 사용하여 게이트 공핍 효과와 관계되는 케이트 절연막 두께의 증가를 예방할 수 있고, 게이트 저항의 감소에 의해 소자 속도를 증가 시킬 수 있다. 추가적으로, 비결정질 실리콘 박막의 결정화 기술로써 사용된 ELA 방법은 SPC (solid phase crystallization) 방법과 SLS (sequential lateral solidification) 방법에 의해 비교되었다. 결과적으로, ELA 방법에 의해 결정화된 다결정 실리콘 박막의 결정도와 표면 거칠기는 SPC와 SLS 방법에 비해 개선되었다. 또한, 우리는 ELA 결정화 방법에 의한 다결정 실리콘 박막 트랜지스터로부터 우수한 소자 특성을 얻었다.

• Korean Journal of Applied Biomechanics
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• v.23 no.2
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• pp.117-123
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• 2013

The purpose of this study was to provide data to increase the success rate of penalty kicks through quantifying the shape of skilled kicks by performing a kinematic analysis on the change of movement during the kicking phase which the goalkeeper uses as a vital clue. Three high definition video cameras(GR-HD1KR, JVC, Japan) were used for the study and 18 reflective markers were attached to the body joints. Corners of the goal, difficult for goalkeepers to block, were set as aims and 1 m by 1.2 m targets were installed. Each subject had five sets of kicks at random, and the analysis was done on the movements that hit the target. Time, speed of the right lower limb's center of mass, joint angle, and angular velocity were chosen as factors and the results of the analysis showed statistical significance. The player taking a penalty kick should train to avoid leaning one's body towards the kicking direction and change the angle of the right foot right before the impact to decide the direction of the ball. The goalkeeper can increase the save success rate by studying the angle of the kicker's body and the right foot as well as the timing of the kick.

• Korean Journal of Applied Biomechanics
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• v.26 no.4
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• pp.333-341
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• 2016

Objective: The purpose of this study was to assess the consistency of the gliding and push-off motion for single leg skating from the first to fourteenth steps. We hypothesized that: 1) there would be no difference in stroke trajectory, step rate, and cycle rate between the left and right steps of gliding; and 2) there would be a difference in the resultant velocity of toe push-off and the horizontal velocity of the center of mass after six step push-offs. Method: The study included five male 500-m speed skaters (mean height, $1.80{\pm}0.02m$; mean weight, $76.8{\pm}3.96kg$; record, $35.83{\pm}0.30sec$; 100-m record, <9.97 sec). Data were collected from the first to fourteenth steps (40 m) and recorded using five digital JVC GR-HD1KR video cameras (Victor Co., Japan) operating at a sampling frequency of 60 fields/sec and shutter speed of 1/500 sec. For each film frame, the joint positions were digitized using the KWON3D motion analyzer. Position data were filtered with low-pass Butterworth $4^{th}$ order at the cut-off frequency of 7.4 Hz. Results: The right toe of the skating trajectories at $2^{nd}$, $5^{th}$, and $7^{th}$ strokes differed from those of the left toe. The angles of the right and left knee demonstrated unbalanced patterns from the flexion and extension legs. The step and cycle rates of the right and left leg differed from the start until 20 m. The resultant velocities of the toe at the push-off phase and of the body mass center diverged before the six push-offs. Conclusion: This study's findings indicate that the toe of skating trajectory on left and right sliding after push-off should maintain a symmetrical trajectory. The resultant velocity of toe push-off and horizontal velocity from the center of body need to be separated after about six step push-offs.