• Journal of Life Science
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• v.17 no.6 s.86
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• pp.822-824
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• 2007

The efficient system for plant regeneration from cotyledon and primary feat explants of lettuce was established. Plant regeneration efficiency was shown 91.3% from cotyledon and 85.9% from primary leaf explants of variety 'Jungtongpogi' in KN medium. Plant regeneration efficiency was also estimated with various plant regeneration media in variety' Chungchima', which was lowest plant regeneration efficient showing 35.4% from cotyledon and 30.3% from prima leaf explants in KN medium. Kl medium increased 77.9% and 80.7% of plant regeneration efficiencies from cotyledon and primary leaf explants of variety 'Jungtongpogi' were cultured on KN medium. In case of varie쇼 ‘Chungchima', efficient plant regeneration was shown when primary leaf explants were cultured on SH and KI media.

• Journal of Broadcast Engineering
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• v.23 no.6
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• pp.768-779
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• 2018

In this paper, we propose a novel video quality function for video processing based on MSSSIM to select an appropriate video processing filter and to accommodate multiple processing filters to each pixel block in a picture frame by a mathematical selection law so as to maintain video quality and to reduce the bitrate of compressed video. In viewpoint of video compression, since the properties of video quality and bitrate is different for each picture of video frames and for each areas in the same frame, it is difficult for the video filter with single property to satisfy the object of increasing video quality and decreasing bitrate. Consequently, to maintain the subjective video quality in spite of decreasing bitrate, we propose the methodology about the MSSSIM as the measure of subjective video quality, the KL-Divergence as the measure of bitrate, and the combination method of those two measurements. Moreover, using the proposed combinatorial measurement, when we use the multiple image filters with mutually different properties as a pre-processing filter for video, we can verify that it is possible to compress video with maintaining the video quality under decreasing the bitrate, as possible.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.386-395
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• 2007

A total of 27 strains of Vibrio parahaemolyticus (18 strains isolated from Korea and 9 strains from Japan) were serotyped and examined for biochemical characteristics, antimicrobial susceptibility patterns, cytotoxicity assay, thermostable direct hemolysin (TDH) production and molecular epidemiology. Using polymerase chain reaction (PCR) method and DNA probe hybridization method, the strains were tested for toxR, tdh, trh and ORF 8 genes. The V. parahaemolyticus isolated from patients were belonged to 8 different serotypes : O3:K6, O1:K38, O3:K57, O4:K9, O4:Kl2, O4:K68, O5:Kl5 and O6:K46. Urease-positive strain possessed the trh gene, and conversely, urease-negative strains lacked the gene, indicating that urease production by V. parahemolyticus strains strongly correlates with the possession of the trh gene. Most strains showed multiple resistant to more than three antibiotics and the antibiogram could be classified into 6 group (I to VI). All of the O3:K6 strains isolated in South Korea and Japan producted TDH at high levels. The TDH titers ranged between 256 and 2.048, and the average titer was 1009. To distinguish the new and increasingly common V. parahaemolyticus strains from clinical isolates, ORF 8 is a useful genetic marker. After Southern hybridization, the HindIII restriction fragment patterns of the tdh gene were grouped one type, respectively. One type showed two bands one of which was 4.3kb and the other was 11.5kb in size. Variation between the O3:K6 serotype are minor when compared to the differences seen with the non O3:K6 strains. The migration patterns of Not I -digested of the total DNA of the O3:K6 strains were similar, and only slight variations were observed between the serotypes. By contrast, the O3:K6 strains and non O3:K6 had markedly different profiles. In conclusion, Random amplified polymorphic DNA (RAPD) profile using appropriate primers was an effective epidemiological marker.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.225-230
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• 2004

A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer concentration in various culture conditions including batch and high-cell-density fed-batch cultures. In the fed-batch culture, the induction around 6 h after initiation of the DO-stat fed-batch culture resulted in the highest expression level of rKI-3 among the induction points examined. The highest demand of oxygen at this point was crucial for the maximum expression level of rKI-3. As the lactose concentration increased, the expression level also increased, though the expression level showed a plateau above a concentration of 14 mM of lactose. Lactose acted less specifically than IPTG since most of it was hydrolyzed to glucose and galactose. However, using lactose, the cell growth and the maximum expression level of rKl-3 increased by 20% and 24%, respectively, compared with those using IPTG in the fed-batch culture. The lactose seemed to be hydrolyzed by intracellular and extracellular $\beta$-galactosidase liberated by cell lysis at the same time. Residual concentration of glucose was maintained to a a limit of detection by high performance liquid chromatography, and galactose was not consumed by the host strain Escherichia coli BL2l(DE3).

• Korean Journal of Microbiology
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• v.44 no.2
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• pp.156-163
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• 2008

In this study, we isolated 32 strains of kerosene degrading bacteria from oil contaminated soil by enrichment culture. Isolates were screened for kerosene degradation efficiencies and K14 were selected which had the highest removal efficiency for 1,000 mg/L of kerosene. K14 were identified as Pseudomonas aeruginosa by morphological, biochemical test and 16S rDNA analysis. The optimal culture condition were determined as initial inoculated cell concentration, 1.0 g/L; substrate concentration, 1,000 mg/L; temperature $30^{\circ}C$; pH 7. When we enforced batch test in this condition, K14 degraded 72% of kerosene with 1,000 mg/L during 72 hr. And, at low concentration (200 mg/L), K14 degraded 95.8% of kerosene during 48 hr. As a result, kerosene biodegradation by Pseudomonas aeruginosa K14 could be useful for clean up of groundwater and soil contaminated with crude oil.

• Journal of the Korean Mathematical Society
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• v.43 no.6
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• pp.1199-1217
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• 2006

The main purpose of this paper is using the properties of Gauss sums, primitive characters and the mean value theorems of Dirichlet L-functions to study the hybrid mean value of the T-th hyper-Kloosterman sums Kl(h, k+1, r;q) and the hyper Cochrane sums C(h, q; m, k), and give an interesting mean value formula.

• Korean Journal of Poultry Science
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• v.25 no.4
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• pp.161-167
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• 1998

This experiment was carried out to develope the production of specific yolk antibody from laying hens immunized with antigens from Salmonella enteritidis. Antigenic protein isolated from the flagella of Salmonella enteritidis, determined by SDS-PAGE, was pure and has a molecular mass of approximately 54.6 kDa. It was observed that the antibody titers both in egg yolk and serum were performed at 2 weeks after immunization with flagella antigen to the laying hen. And the level was increased gradually to 6 weeks after immunization. At the time of 6 weeks, the antibody titer of yolk showed higher than that of serum. According to the results of specificity test(ELISA), the yolk antibody did not react with different bacterial strains(S. choleraesuis, ETEC Kl2:K99, K88,987P), but reacted only with S. enteritidis strain. The contents of immunoglobulin(IgY) in an egg yolk was 106mg approximately. By the isolation procedure of IgY from the egg yolk, 88.3 percent of IgY content was recovered in this study.

• Food Science and Preservation
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• v.5 no.3
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• pp.292-298
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• 1998

The isolates from putrefying soybean curd were identified as Acinetobacter calcoaceticus, Bacillus cereus, Bacillus sp., Cardiobacterium sp., Escherichia coli, Klebsiella pneumoniae, Pantoea sp., Salmonella typhimurium, Staphylococcus aureus, Xenorhabdus luminescens, Yersinia sp.. The existence percentages of the bacteria from putrefying soybean curd at room temperature storage were Bacillus cereus J55 23.57%, Xenorhabdus luminescens J48 22.73%, Acinetobacter calcoaceticus J61 22.26%, Klebsiella pneumoniae J62 21.25%, Salmonella typhimurium J51 2.87%, Pantoea sp. J57 2.65%, Bacillus sp. J58 1.43%, Cardiobacterium sp. J54 1.26%, Escherichia coli J53 1.20%, Staphvlococcus aureus J6O 0.93%, Yersinia sp. J50 0.05%, respectively. Four out of eleven bacteria as B. cereu J55, X. luminescens J48, Ac. calcoaceticus J61, Kl. pneumoniae J62 putrefied soybean curd and those bacteria produce amylase or proteinase as a extracellular enzyme. But S. typhimurium J51, Pantoea sp. J57, Bacillus sp. J58, Cardiobacterium sp. J54, E. coli 153, St. aureus J60, Yersinia sp. J50 were not putrefied soybean curd. The isolates detected to resistant on various antimicrobial agents. The majority were resistant to aminoside antiboitics as amicacin, gentamicin, tobramycin and were susceptible to ${\beta}$-lactamine antibiotics as penicillin G, oxacillin, cephalothin cefazolin, cefamandole.

• YAKHAK HOEJI
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• v.56 no.3
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• pp.186-190
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• 2012

Nuclear factor-${\kappa}B$ (NF-${\kappa}B$) has been considered as one of the major targets for therapeutic agents of diverse human diseases. In the previous studies, 6-hydroxy-7-methoxychroman-2-carboxylic acid N-phenylamide (KL-1156) and chroman-2-carboxylic acid N-(4-chlorophenyl)amide were identified as good inhibitors of NF-${\kappa}B$ activation. In this continuous study, we describe the synthesis and NF-${\kappa}B$ inhibitory activities of chroman derivatives containing N-heteroaryl groups for exploration of SAR (structure-activity relationship). In addition, inhibitory effects of cell proliferation are evaluated against human cancer cell lines (NCI-H23 and PC-3).

• Journal of the Korean Society of Food Culture
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• v.4 no.3
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• pp.287-292
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• 1989

우리나라는 예전부터 쌀 생산의 명승지였으며 국민은 벼농사를 주요생업으로 삼아 왔기 때문에 자연히 쌀을 원료로 한 술이 나오게 된 것은 당연한 이치이다. 탁주는 일반인이 아는 바와 같이 쌀과 누룩으로 제조한 것이 전통 민속주라 할 수 있겠다. 일제시대에 들어와 1920년경에 일본에서 종국을 들여와 청주제조 및 조미료 제조에 약간씩 이용되었고 해방을 전 후하여 탁 약주에 널리 보급되기 시작하였다. 근대의 탁 약주 출고 및 원료의 변천을 보면 1. 1960년대부터 매년 출고량이 신장되어 왔으며 1973년도 출고량 1,613,147kl가 출고되어 전주류 구성비율은 78.6% 이상에 이르게 되었으나, 2. 1974년부터 1975년(2년간) 사이에 원료변화가 4차례나 바뀌어짐에 따라 1974년도에 소맥 50%, 대맥(보리쌀)50%를 사용하면서부터 출고량이 감소되기 시작하였다. 3. 1988년말 현재에 이르러 출고량은 761,284kl로 53%가 감소되었고 주류 구성비율은 30%내로 이르게 되었다. 우리나라 국민은 누구나 민속주 하면 탁주를 연상한다. 그러나 정부의 식량난 정책에 따라 탁 약주 제조원료 변화는 갖은 수난을 거쳐 오늘에 이르게 되었으나 이제는 경제가 발전되어 국민의 의 식 주도 많이 변화되어지고 있는 바 지난날과 같은 원료변화는 다시는 없어야겠다. 끝으로 탁 약주 제조자의 바램이라면 제조원료를 자유롭게 선택하여 상품을 다양화하고 소비자의 선택에 따라 전통 민속주의 맛을 음미할 수 있는 때가 오기를 바란다.